Forced Abstinence from Volitional Ethanol Intake Drives a Vulnerable Period of Hyperexcitability in BNST-Projecting Insular Cortex Neurons DOI Creative Commons
Anne Taylor, Danielle N. Adank,

Phoebe A. Young

et al.

Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 44(4), P. e1121232023 - e1121232023

Published: Nov. 22, 2023

The insular cortex (IC) integrates sensory and interoceptive cues to inform downstream circuitry executing adaptive behavioral responses. IC communicates with areas involved canonically in stress motivation. projections govern ethanol recruitment of bed nucleus the stria terminalis (BNST) activity necessary for emergence negative affective behaviors during alcohol abstinence. Here, we assess impact chronic drinking forced abstinence (CDFA) volitional home cage intake paradigm on synaptic excitable properties neurons that project BNST (IC →BNST ). Using whole-cell patch-clamp electrophysiology, investigated 24 h or 2 weeks following (FA) female C57BL6/J mice. We find cells are transiently more acute withdrawal. In contrast, vivo exposure via intraperitoneal injection, ex wash, FA from a natural reward (sucrose) all failed alter excitability. situ hybridization studies revealed at post BK channel mRNA expression is reduced IC. Further, pharmacological inhibition channels mimicked phenotype, while activation was able decrease AP firing control subjects. All together these data suggest novel mechanism homeostatic plasticity occurs drinking.

Language: Английский

Sex-dependent, lateralized engagement of anterior insular cortex inputs to the dorsolateral striatum in binge alcohol drinking DOI Open Access
David L. Haggerty, Brady K. Atwood

Published: Aug. 20, 2024

How does alcohol consumption alter synaptic transmission across time, and do these alcohol-induced neuroadaptations occur similarly in both male female mice? Previous work shows that anterior insular cortex (AIC) projections to the dorsolateral striatum (DLS) are uniquely sensitive male, but not mice, play a role governing binge mice. Here, by using high-resolution behavior data paired with in-vivo fiber photometry, we show how similar levels of intake achieved via different behavioral strategies sex, inter-drinking session thirst states predict future intakes females, males. Further, presynaptic calcium activity recorded from AIC inputs DLS 3 weeks water followed change across, fluid, brain circuit lateralization. By time-locking peri-initiation drinking events also into left robustly encode behaviors relative right males, females. These findings suggest fluid-, sex- lateralization-dependent for engagement further contextualize at DLS.

Language: Английский

Citations

1
Drinking history dependent functionality of the dorsolateral striatum on gating alcohol and quinine-adulterated alcohol front-loading and binge drinking DOI Creative Commons
Meredith R. Bauer,

Megan M. McVey,

Stephen L. Boehm

et al.

Alcohol, Journal Year: 2022, Volume and Issue: 105, P. 43 - 51

Published: Oct. 12, 2022

Language: Английский

Citations

6
Sex-dependent, lateralized engagement of anterior insular cortex inputs to the dorsolateral striatum in binge alcohol drinking DOI Open Access
David L. Haggerty, Brady K. Atwood

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 24, 2023

Abstract How does alcohol consumption alter synaptic transmission across time, and do these alcohol-induced neuroadaptations occur similarly in both male female mice? Previous work shows that anterior insular cortex (AIC) projections to the dorsolateral striatum (DLS) are uniquely sensitive male, but not mice, play a role governing binge mice. Here, by using high-resolution behavior data paired with in-vivo fiber photometry, we show how similar levels of intake achieved via different behavioral strategies sex, inter-drinking session thirst states predict future intakes females, males. Further, presynaptic calcium activity recorded from AIC inputs DLS 3 weeks water followed change across, fluid, brain circuit lateralization. By time-locking peri-initiation drinking events also into left robustly encode behaviors relative right males, females. These findings suggest fluid-, sex- lateralization-dependent for engagement further contextualize at DLS.

Language: Английский

Citations

3
Circuit dysfunctions of associative and sensorimotor basal ganglia loops in alcohol use disorder: insights from animal models DOI Creative Commons
Giacomo Sitzia, David M. Lovinger

Addiction Neuroscience, Journal Year: 2022, Volume and Issue: 5, P. 100056 - 100056

Published: Dec. 8, 2022

Persons that develop Alcohol Use Disorder (AUD) experience behavioral changes include compulsion to seek and take alcohol despite its negative consequences on the person's psychosocial, health economic spheres, inability limit intake a emotional/ motivational state emerges during withdrawal. During all stages of AUD executive functions, i.e. ability direct their behavior towards goal, working memory cognitive flexibility are eroded. Animal models recapitulate aspects action selection impairment offer opportunity benchmark underlying circuit mechanisms. Here we propose circuit-based approach research focusing recent advances in analysis, neuroanatomy, genetics, physiology guide future field.

Language: Английский

Citations

5
A novel inhibitory corticostriatal circuit that expresses mu opioid receptor-mediated synaptic plasticity DOI Creative Commons
Braulio Muñoz, Brady K. Atwood

Neuropharmacology, Journal Year: 2023, Volume and Issue: 240, P. 109696 - 109696

Published: Sept. 1, 2023

Language: Английский

Citations

2
Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder DOI
Tiange Xiao, Yueyi Chen,

Alyssa Boisvert

et al.

Journal of Visualized Experiments, Journal Year: 2023, Volume and Issue: 196

Published: June 23, 2023

Alcohol use disorder (AUD) is a chronic alcohol-related that typically presents as uncontrolled drinking and preoccupation with alcohol. A key component of AUD research using translationally relevant preclinical models. Over the past several decades, variety animal models have been used to study AUD. One prominent model intermittent ethanol vapor exposure (CIE) model, which well-established approach for inducing alcohol dependence in rodents through repeated cycles via inhalation. To mice, CIE paired voluntary two-bottle choice (2BC) water measure escalation drinking. The 2BC/CIE procedure involves alternating weeks 2BC CIE, repeat until achieved. In present study, we outline procedures performing 2BC/CIE, including daily chamber, provide an example escalated C57BL/6J mice this approach.

Language: Английский

Citations

2
Dorsomedial Striatal Glutamatergic Transmission Inhibits Binge Drinking in Selectively Bred Crossed High Alcohol Preferring Mice DOI Creative Commons
Meredith R. Bauer,

Megan M. McVey,

Yanping Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 17, 2024

Abstract Crossed high alcohol preferring (cHAP) mice have been selectively bred to consume considerable amounts of resulting in binge drinking. The dorsal striatum (DS) is a brain region involved action selection where the dorsomedial (DMS) goal-directed and dorsolateral (DLS) habitual selection. Alcohol use disorder (AUD) may involve disruption balance between DMS DLS. While DLS drinking, reliance on drinking has not investigated cHAP mice. We previously demonstrated that glutamatergic activity necessary for binge-like C57BL/6J mice, another mouse. Because this, we hypothesized would gate underwent bilateral cannulation into or were allowed free-access 20% two-hours each day 11 days. Mice microinjected with AMPA receptor (AMPAR) antagonist, NBQX, immediately prior access. AMPAR protein expression was also assessed separate group animals DS subregions following an 11-day history. found intra-DMS (but intra-DLS) alters NBQX increasing consumption. ratio GluA1 GluA2 differs across subregions. Together, these findings suggest serve limit

Language: Английский

Citations

0
Alcohol consumption does not impact delta and kappa opioid receptor-mediated synaptic depression in dorsolateral striatum of adult male mice. DOI
Braulio Muñoz, Brady K. Atwood

Alcohol, Journal Year: 2024, Volume and Issue: 119, P. 89 - 95

Published: June 12, 2024

Language: Английский

Citations

0
Chronic alcohol induces subcircuit-specific striatonigral plasticity enhancing the sensorimotor basal ganglia role in action execution DOI Creative Commons
Giacomo Sitzia, Sebastiano Bariselli, Alexa L. Gracias

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(26)

Published: June 28, 2024

Functional deficits in basal ganglia (BG) circuits contribute to cognitive and motor dysfunctions alcohol use disorder. Chronic exposure alters synaptic function neuronal excitability the dorsal striatum, but it remains unclear how affects BG output that is mediated by substantia nigra pars reticulata (SNr). Here, we describe a subpopulation-specific organization of striatal subthalamic (STN) inputs medial lateral SNr. (CIE) potentiated dorsolateral striatum (DLS) did not change dorsomedial STN Chemogenetic inhibition DLS direct pathway neurons revealed an enhanced role for execution instrumental lever-pressing task. Overall, reveal subregion-specific onto SNr find DLS-SNr are accompanied altered control action following CIE.

Language: Английский

Citations

0
Sex-dependent, lateralized engagement of anterior insular cortex inputs to the dorsolateral striatum in binge alcohol drinking DOI Open Access
David L. Haggerty, Brady K. Atwood

Published: June 28, 2024

How does alcohol consumption alter synaptic transmission across time, and do these alcohol-induced neuroadaptations occur similarly in both male female mice? Previous work shows that anterior insular cortex (AIC) projections to the dorsolateral striatum (DLS) are uniquely sensitive male, but not mice, play a role governing binge mice. Here, by using high-resolution behavior data paired with in-vivo fiber photometry, we show how similar levels of intake achieved via different behavioral strategies sex, inter-drinking session thirst states predict future intakes females, males. Further, presynaptic calcium activity recorded from AIC inputs DLS 3 weeks water followed change across, fluid, brain circuit lateralization. By time-locking peri-initiation drinking events also into left robustly encode behaviors relative right males, females. These findings suggest fluid-, sex- lateralization-dependent for engagement further contextualize at DLS.

Language: Английский

Citations

0