PubMed,
Journal Year:
2023,
Volume and Issue:
25(2), P. 140 - 146
Published: Feb. 15, 2023
To
study
new
biomarkers
for
the
early
diagnosis
of
retinopathy
prematurity
(ROP)
by
analyzing
differences
in
blood
metabolites
based
on
liquid
chromatography-tandem
mass
spectrometry
(LC-MS/MS)
and
metabolomics.Dried
spots
were
collected
from
21
infants
with
ROP
(ROP
group)
without
(non-ROP
who
hospitalized
Sixth
Affiliated
Hospital
Sun
Yat-sen
University
January
2013
to
December
2016.
LC-MS/MS
was
used
measure
metabolites,
orthogonal
partial
least
squares-discriminant
analysis
search
differentially
expressed
biomarkers.There
a
significant
difference
metabolic
profiles
between
non-ROP
groups.
The
pattern
recognition
analysis,
Score-plot,
weight
obtained
10
amino
acids
relatively
large
difference.
Further
statistical
showed
that
group
had
increases
levels
glutamic
acid,
leucine,
aspartic
ornithine,
glycine
compared
(P<0.05).
receiver
operating
characteristic
curve
acid
ornithine
highest
value
diagnosing
ROP.Blood
preterm
are
different
those
ROP.
Glutamic
markers
combined
metabolomics
has
potential
application
identification
ROP.目的:
应用液相色谱-串联质谱技术（liquid
chromatography
tandem
spectrometry，LC-MS/MS）和代谢组学方法探讨早产儿视网膜病（retinopathy
prematurity，ROP）患儿出生血代谢产物差异，寻找ROP早期诊断的新的生物标记物。方法:
收集2013年1月—2016年12月中山大学附属第六医院住院的21例ROP患儿（ROP组）及同期21例非ROP患儿（非ROP组）干血片标本，利用LC-MS/MS进行代谢产物测定，运用正交偏最小二乘判别分析法（orthogonal
squares
discriminant
analysis，OPLS-DA）寻找差异物质和生物标记物。结果:
ROP组和非ROP组患儿血代谢谱有明显差异，经模式识别分析、代谢物得分图（Score-plot，S-plot）、权重分析初步得出10个差异较大的氨基酸。进一步统计分析发现ROP组患儿血谷氨酸、亮氨酸、天冬氨酸、鸟氨酸和甘氨酸水平明显高于非ROP组，差异有统计学意义（P<0.05）。受试者工作特征曲线分析显示，谷氨酸及鸟氨酸对ROP诊断价值最高。结论:
ROP患儿与非ROP患儿比较血代谢产物具有明显差异，谷氨酸及鸟氨酸是诊断ROP的代谢标志物。LC-MS/MS结合代谢组学分析方法在ROP早期识别与诊断中具有潜在的应用价值。.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(10), P. 5503 - 5503
Published: May 18, 2024
Appropriate
nutrients
are
essential
for
cellular
function.
Dietary
components
can
alter
the
risk
of
systemic
metabolic
diseases,
including
cardiovascular
cancer,
diabetes,
and
obesity,
also
affect
retinal
age-related
macular
degeneration,
diabetic
retinopathy,
glaucoma.
have
been
assessed
prevention
or
treatment
ischemic
diseases
aging.
In
this
article,
we
review
clinical
experimental
evidence
concerning
potential
some
nutritional
supplements
to
prevent
treat
provide
further
insights
into
therapeutic
effects
supplementation
on
retinopathies.
We
will
roles
in
preventing
protecting
against
diseases.
Redox Biology,
Journal Year:
2023,
Volume and Issue:
66, P. 102865 - 102865
Published: Aug. 29, 2023
Oxygen
therapy
is
common
during
the
neonatal
period
to
improve
survival,
but
it
can
increase
risk
of
oxygen
toxicity.
Hyperoxia
damage
multiple
organs
and
systems
in
newborns,
commonly
causing
lung
conditions
such
as
bronchopulmonary
dysplasia
pulmonary
hypertension,
well
other
organs,
including
brain,
gut,
eyes.
These
are
collectively
referred
newborn
radical
disease
indicate
multi-system
caused
by
hyperoxia.
also
lead
changes
metabolic
pathways
production
abnormal
metabolites
through
a
process
called
reprogramming.
Currently,
some
studies
have
analyzed
mechanism
reprogramming
induced
The
focus
has
been
on
mitochondrial
oxidative
stress,
dynamics,
multi-organ
interactions,
lung–gut,
lung–brain,
brain–gut
axes.
In
this
article,
we
provide
an
overview
major
pathway
reported
hyperoxia-associated
diseases
explore
potential
mechanisms
Metabolic
hyperoxia
cause
disorders
glucose,
lipid,
amino
acid
metabolism.
Moreover,
may
predict
occurrence
disease,
suggesting
their
therapeutic
targets.
Although
requires
further
elucidation,
mitochondria
gut–lung–brain
axis
play
key
role
Journal of Personalized Medicine,
Journal Year:
2024,
Volume and Issue:
14(4), P. 379 - 379
Published: March 31, 2024
Aim:
The
temporal
avascular
area
of
the
retina
and
duration
mechanical
ventilation
(DMV)
may
predict
need
to
treat
retinopathy
prematurity
(ROP).
This
study
considers
whether
rate
retinal
vascularisation
related
risk
factors
should
be
included
in
a
predictive
model
for
ROP
treatment.
Methods:
single-centre,
observational
retrospective
case–control
was
conducted
on
276
preterm
infants
an
screening
programme.
All
had
undergone
at
least
three
examinations
fundus.
main
outcome
measures
considered
were
DMV
(in
days
treatment),
disc
diameters,
DD)
(DD/week).
Results:
multivariate
logistic
that
best
explains
treatment
(R2
=
63.1%)
has
significant
factors:
each
additional
day
(OR,
1.05
[95%
CI,
1.02–1.09];
p
0.001);
DD
2.2
1.7–2.9];
<
0.001)
<0.5
DD/week
19.0
6.5–55.5];
0.001).
Two
tables
are
presented
calculating
expected
according
these
factors.
Conclusions:
A
greater
DMV,
broad
first
binocular
slow
strongly
we
describe
must
validated
externally
other
centres.
Angiogenesis,
Journal Year:
2023,
Volume and Issue:
26(3), P. 409 - 421
Published: March 21, 2023
Abstract
Hyperglycemia
in
early
postnatal
life
of
preterm
infants
with
incompletely
vascularized
retinas
is
associated
increased
risk
potentially
blinding
neovascular
retinopathy
prematurity
(ROP).
Neovascular
ROP
(Phase
II
ROP)
a
compensatory
but
ultimately
pathological
response
to
the
suppression
physiological
retinal
vascular
development
I
ROP).
neonatal
mice
which
suppresses
growth
decreased
expression
systemic
and
fibroblast
factor
21
(FGF21).
FGF21
administration
promoted
deficiency
suppressed
vessel
growth.
serum
adiponectin
(APN)
levels
loss
APN
abolished
promotion
development.
Blocking
mitochondrial
fatty
acid
oxidation
also
protection
against
delayed
Clinically,
developing
severe
(versus
non-severe
had
lower
total
lipid
intake
more
parenteral
less
enteral
during
first
4
weeks
life.
Our
data
suggest
that
increasing
presence
adequate
lipids
may
help
prevent
Phase
(and
therefore
disease).
Diagnostics,
Journal Year:
2023,
Volume and Issue:
13(12), P. 2105 - 2105
Published: June 18, 2023
Retinopathy
of
Prematurity
(ROP)
is
a
major
cause
blindness
in
premature
infants.
This
study
aimed
to
evaluate
the
association
between
inflammatory
markers
and
ROP
development
extremely
very
neonates
identify
potential
biomarkers
for
risk
prediction.
prospective
was
conducted
from
January
2021
2023
two
clinical
hospitals
associated
with
"Victor
Babes"
University
Medicine
Pharmacy
Timisoara.
The
population
comprised
gestational
age
less
than
32
weeks.
Various
markers,
including
total
white
blood
cell
count,
polymorphonuclear
leukocytes,
C-reactive
protein,
interleukin-6,
lactate
dehydrogenase,
were
analyzed
samples
collected
at
birth
three
days
postnatally.
diagnosed
classified
following
International
Classification
Prematurity.
included
48
neonates,
12
Extremely
Premature
Infants
(EPI),
36
Very
(VPI).
EPI
group
had
significantly
higher
mean
interleukin-6
dehydrogenase
levels
postnatally
VPI
group.
protein
Umbilical
cord
inflammation
severity
found
have
statistically
significant
positive
correlation.
Half
EPIs
moderate
severe
ROP,
more
duration
oxygen
supplementation,
mechanical
ventilation,
Continuous
Positive
Airway
Pressure
(CPAP),
28
weeks,
umbilical
or
above
stage
3
factors
developing
2
above.
Elevated
CRP
IL-6
also
an
increased
above,
highlighting
their
as
suggests
neonates.
These
findings
could
contribute
identification
prediction,
improving
early
diagnosis
intervention
strategies
this
condition.
Angiogenesis,
Journal Year:
2024,
Volume and Issue:
27(4), P. 691 - 699
Published: Aug. 3, 2024
Abstract
Objective
Pathological
retinal
neovascularization
is
vision-threatening.
In
mouse
oxygen-induced
retinopathy
(OIR)
we
sought
to
define
mitochondrial
respiration
changes
longitudinally
during
hyperoxia-induced
vessel
loss
and
hypoxia-induced
neovascularization,
test
interventions
addressing
those
prevent
neovascularization.
Methods
OIR
was
induced
in
C57BL/6J
mice
vasculature
examined
at
maximum
neovessel
formation.
We
assessed
total
proteome
the
ratio
of
nuclear
DNA
copy
numbers
(mtDNA/nDNA)
vs.
control
retinas,
oxygen
consumption
rates
(OCR)
ex
vivo
retinas
(BaroFuse).
Pyruvate
vehicle
supplemented
either
prior
or
Results
global
proteomics
showed
decreased
peak
OCR
mtDNA/nDNA
were
also
suggesting
impaired
respiration.
pyruvate
administration
but
not
formation
(in
line
with
activity
time
course)
suppressed
NV.
Conclusions
Mitochondrial
energetics
NV
OIR.
Appropriately
timed
supplementation
may
be
a
novel
approach
neovascular
diseases.
