Descending control of motor sequences in

Current Opinion in Neurobiology, Journal Year: 2023, Volume and Issue: 84, P. 102822 - 102822

Published: Dec. 13, 2023

The descending neurons connecting the fly's brain to its ventral nerve cord respond sensory stimuli and evoke motor programs of varying complexity. Anatomical characterization their synaptic connections suggests how these circuits organize movements, while optogenetic manipulation activity reveals what behaviors they can induce. Monitoring responses or during behavior performance indicates information may encode. Recent advances in all three approaches make an excellent place better understand sensorimotor integration transformation required for nervous systems govern sequences that constitute animal behavior.

Language: Английский

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Building and integrating brain-wide maps of nervous system function in invertebrates

Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 86, P. 102868 - 102868

Published: April 3, 2024

The selection and execution of context-appropriate behaviors is controlled by the integrated action neural circuits throughout brain. However, how activity coordinated across brain regions, nervous system structure enables these functional interactions, remain open questions. Recent technical advances have made it feasible to build brain-wide maps function, such as maps, connectomes, cell atlases. Here, we review recent progress in this area, focusing on C. elegans D. melanogaster, work has produced global systems. We also describe circuit motifs elucidated studies specific networks, which highlight complexities that must be captured accurate models whole-brain function.

Language: Английский

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Evolutionarily conserved roles of cholecystokinin signaling

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 21 - 70

Published: Jan. 1, 2025

Language: Английский

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Cholecystokinin in nociception and pain

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 557 - 581

Published: Jan. 1, 2025

Language: Английский

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Feedback inhibition by a descending GABAergic neuron regulates timing of escape behavior in Drosophila larvae

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Jan. 26, 2024

Escape behaviors help animals avoid harm from predators and other threats in the environment. Successful escape relies on integrating information multiple stimulus modalities (of external or internal origin) to compute trajectories toward safe locations, choose between actions that satisfy competing motivations, execute strategies ensure survival. To this end, must be adaptive. When a Drosophila melanogaster larva encounters noxious stimulus, such as focal pressure parasitic wasp applies larval cuticle via its ovipositor, it initiates characteristic response. The sequence consists of an initial abrupt bending, lateral rolling, finally rapid crawling. Previous work has shown detection stimuli primarily class IV multi-dendritic arborization neurons (Class neurons) located beneath body wall, more recent studies have identified several important components nociceptive neural circuitry involved rolling. However, mechanisms underlie rolling-escape remain unclear. Here, we present both functional anatomical evidence suggesting bilateral descending within subesophageal zone D. play crucial role regulating termination rolling subsequent transition We demonstrate these (designated SeIN128) are inhibitory receive inputs second-order interneuron upstream (Basin-2) ascending neuron downstream Basin-2 (A00c). Together with optogenetic experiments showing co-activation SeIN128 influence temporal dynamics our findings collectively suggest ensemble SeIN128, Basin-2, A00c forms GABAergic feedback loop onto which inhibits thereby facilitates shift

Language: Английский

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Activation of the G protein‐coupled sulfakinin receptor inhibits blood meal intake in the mosquito Aedes aegypti

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(15)

Published: Aug. 7, 2024

Abstract Little is known about the blood‐feeding physiology of arbovirus vector Aedes aegypti although this type mosquito to transmit infectious diseases dengue, Zika, yellow fever, and chikungunya. Blood feeding in female A. essential for egg maturation transmission disease agents between human subjects. Here, we identify sulfakinin receptor gene SKR from genome show that expressed at different developmental stages varied anatomical localizations adult (at three days after eclosion), with particularly high expression CNS. Knockingdown results increased blood meal intake, but microinjection thorax peptide 1 2 both inhibits dose dependently intake (and delays time course intake), which reversible antagonist. Sulfakinin ectopically mammalian cells CHO‐K1 responds stimulation persistent calcium spikes, blockable These data together suggest activation Gq protein‐coupled (i.e., calcium‐mobilizing) mosquitoes could serve as a strategic node future control reproduction/population transmission.

Language: Английский

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Nociception in fruit fly larvae

Frontiers in Pain Research, Journal Year: 2023, Volume and Issue: 4

Published: March 17, 2023

Nociception, the process of encoding and processing noxious or painful stimuli, allows animals to detect avoid escape from potentially life-threatening stimuli. Here, we provide a brief overview recent technical developments studies that have advanced our understanding Drosophila larval nociceptive circuit demonstrated its potential as model system elucidate mechanistic basis nociception. The nervous larva contains roughly 15,000 neurons, which for reconstructing connectivity among them directly by transmission electron microscopy. In addition, availability genetic tools manipulating activity individual neurons advances in computational high-throughput behavior analysis methods facilitated identification neural underlying characteristic nocifensive behavior. We also discuss how neuromodulators may play key role modulating behavioral output. A detailed structure function could insights into organization operation pain circuits mammals generate new knowledge advance development treatment options humans.

Language: Английский

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Feedback inhibition by a descending GABAergic neuron regulates timing of escape behavior in Drosophila larvae

Published: Jan. 26, 2024

Escape behaviors help animals avoid harm from predators and other threats in the environment. Successful escape relies on integrating information multiple stimulus modalities (of external or internal origin) to compute trajectories toward safe locations, choose between actions that satisfy competing motivations, execute strategies ensure survival. To this end, must be adaptive. When a Drosophila melanogaster larva encounters noxious stimulus, such as focal pressure parasitic wasp applies larval cuticle via its ovipositor, it initiates characteristic response. The sequence consists of an initial abrupt bending, corkscrew-like rolling, finally rapid crawling. Previous work has shown detection stimuli primarily class IV multi dendritic arborization neurons (Class neurons) located beneath body wall, more recent studies have identified several important components nociceptive neural circuitry involved rolling. However, mechanisms underlie rolling-escape remain unclear. Here we present both functional anatomical evidence suggesting bilateral descending within subesophageal zone D. play crucial role regulating termination rolling subsequent transition We demonstrate these (designated SeIN128) are inhibitory receive inputs second-order interneuron upstream (Basin-2) ascending neuron downstream Basin-2 (A00c). Together with optogenetic experiments showing joint stimulation SeIN128 influence temporal dynamics our findings collectively suggest ensemble SeIN128, Basin-2, A00c forms GABAergic feedback loop onto which inhibits thereby facilitates shift

Language: Английский

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Drosulfakinin signaling encodes early-life memory for adaptive social plasticity

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Dec. 18, 2024

Drosophila establishes social clusters in groups, yet the underlying principles remain poorly understood. Here, we performed a systemic analysis of network behavior (SNB) that quantifies individual distance (SD) group over time. The SNB assessment 175 inbred strains from Genetics Reference Panel showed tight association short SD with long developmental time, low food intake, and hypoactivity. inferiority short-SD individuals was compensated by their culturing. By contrast, isolation silenced beneficial effects interactions adults blunted plasticity under physiological challenges. Transcriptome analyses revealed genetic diversity for traits, whereas reprogrammed select pathways, regardless phenotypes. In particular, deprivation suppressed expression neuropeptide Drosulfakinin ( Dsk ) three pairs adult brain neurons. Male-specific DSK signaling to cholecystokinin-like receptor 17D1 mediated plasticity. fact, transgenic manipulations neuron activity were sufficient imitate state experience. Given functional conservation mammalian homologs, propose animals may have evolved dedicated neural mechanism encode early-life experience transform properties adaptively.

Language: Английский

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Reviewer #3 (Public Review): Feedback inhibition by a descending GABAergic neuron regulates timing of escape behavior in Drosophila larvae

Published: Jan. 26, 2024

Escape behaviors help animals avoid harm from predators and other threats in the environment. Successful escape relies on integrating information multiple stimulus modalities (of external or internal origin) to compute trajectories toward safe locations, choose between actions that satisfy competing motivations, execute strategies ensure survival. To this end, must be adaptive. When a Drosophila melanogaster larva encounters noxious stimulus, such as focal pressure parasitic wasp applies larval cuticle via its ovipositor, it initiates characteristic response. The sequence consists of an initial abrupt bending, corkscrew-like rolling, finally rapid crawling. Previous work has shown detection stimuli primarily class IV multi dendritic arborization neurons (Class neurons) located beneath body wall, more recent studies have identified several important components nociceptive neural circuitry involved rolling. However, mechanisms underlie rolling-escape remain unclear. Here we present both functional anatomical evidence suggesting bilateral descending within subesophageal zone D. play crucial role regulating termination rolling subsequent transition We demonstrate these (designated SeIN128) are inhibitory receive inputs second-order interneuron upstream (Basin-2) ascending neuron downstream Basin-2 (A00c). Together with optogenetic experiments showing joint stimulation SeIN128 influence temporal dynamics our findings collectively suggest ensemble SeIN128, Basin-2, A00c forms GABAergic feedback loop onto which inhibits thereby facilitates shift

Language: Английский

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