Recent Advances in 3D Printing of Smart Scaffolds for Bone Tissue Engineering and Regeneration

Advanced Materials, Год журнала: 2024, Номер 36(34)

Опубликована: Июнь 11, 2024

The repair and functional reconstruction of bone defects resulting from severe trauma, surgical resection, degenerative disease, congenital malformation pose significant clinical challenges. Bone tissue engineering (BTE) holds immense potential in treating these defects, without incurring prevalent complications associated with conventional autologous or allogeneic grafts. 3D printing technology enables control over architectural structures at multiple length scales has been extensively employed to process biomimetic scaffolds for BTE. In contrast inert grafts, next-generation smart possess a remarkable ability mimic the dynamic nature native extracellular matrix (ECM), thereby facilitating regeneration. Additionally, they can generate tailored controllable therapeutic effects, such as antibacterial antitumor properties, response exogenous and/or endogenous stimuli. This review provides comprehensive assessment progress 3D-printed BTE applications. It begins an introduction physiology, followed by overview technologies utilized scaffolds. Notable advances various stimuli-responsive strategies, efficacy, applications are discussed. Finally, highlights existing challenges development implementation scaffolds, well emerging this field.

Язык: Английский

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Engineering Large‐Scale Self‐Mineralizing Bone Organoids with Bone Matrix‐Inspired Hydroxyapatite Hybrid Bioinks

Advanced Materials, Год журнала: 2024, Номер 36(30)

Опубликована: Апрель 20, 2024

Abstract Addressing large bone defects remains a significant challenge owing to the inherent limitations in self‐healing capabilities, resulting prolonged recovery and suboptimal regeneration. Although current clinical solutions are available, they have notable shortcomings, necessitating more efficacious approaches Organoids derived from stem cells show great potential this field; however, development of organoids has been hindered by specific demands, including need for robust mechanical support provided scaffolds hybrid extracellular matrices (ECM). In context, bioprinting technologies emerged as powerful means replicating complex architecture tissue. The research focused on fabrication highly intricate ECM analog using novel bioink composed gelatin methacrylate/alginate methacrylate/hydroxyapatite (GelMA/AlgMA/HAP). Bioprinted facilitate long‐term cultivation progressive maturation extensive bioprinted organoids, foster multicellular differentiation, offer valuable insights into initial stages formation. intrinsic self‐mineralizing quality closely emulates properties natural bone, empowering with enhanced repair both vitro vivo applications. This trailblazing investigation propels field tissue engineering holds promise its translation practical

Язык: Английский

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AI energized hydrogel design, optimization and application in biomedicine

Materials Today Bio, Год журнала: 2024, Номер 25, С. 101014 - 101014

Опубликована: Фев. 29, 2024

Traditional hydrogel design and optimization methods usually rely on repeated experiments, which is time-consuming expensive, resulting in a slow-moving of advanced development. With the rapid development artificial intelligence (AI) technology increasing material data, AI-energized hydrogels for biomedical applications has emerged as revolutionary breakthrough materials science. This review begins by outlining history AI potential advantages using hydrogels, such prediction properties, multi-attribute optimization, high-throughput screening, automated discovery, optimizing experimental design, etc. Then, we focus various supported biomedicine, including drug delivery, bio-inks manufacturing, tissue repair, biosensors, so to provide clear comprehensive understanding researchers this field. Finally, discuss future directions prospects, new perspective research novel applications.

Язык: Английский

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Synthetic biology‐based bacterial extracellular vesicles displaying BMP‐2 and CXCR4 to ameliorate osteoporosis

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(4)

Опубликована: Апрель 1, 2024

Osteoporosis (OP) is a systematic bone disease characterized by low mass and fragile microarchitecture. Conventional treatment for OP has limited efficacy long-term toxicity. Synthetic biology makes bacterial extracellular vesicle (BEVs)-based therapeutic strategies promising alternative the of OP. Here, we constructed recombinant probiotics Escherichia coli Nissle 1917-pET28a-ClyA-BMP-2-CXCR4 (ECN-pClyA-BMP-2-CXCR4), in which BMP-2 CXCR4 were overexpressed fusion with BEVs surface protein ClyA. Subsequently, isolated engineered BEVs-BMP-2-CXCR4 (BEVs-BC) therapy. The BEVs-BC exhibited great targeting vivo. In addition, had good biocompatibility remarkable ability to promote osteogenic differentiation BMSCs. Finally, synthetic biology-based significantly prevented an ovariectomized (OVX) mouse model. conclusion, both bone-targeting bone-forming one-step using biology, provides effective strategy potential industrialization.

Язык: Английский

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The role and applications of extracellular vesicles in osteoporosis

Bone Research, Год журнала: 2024, Номер 12(1)

Опубликована: Янв. 23, 2024

Abstract Osteoporosis is a widely observed condition characterized by the systemic deterioration of bone mass and microarchitecture, which increases patient susceptibility to fragile fractures. The intricate mechanisms governing homeostasis are substantially impacted extracellular vesicles (EVs), play crucial roles in both pathological physiological contexts. EVs derived from various sources exert distinct effects on osteoporosis. Specifically, released osteoblasts, endothelial cells, myocytes, mesenchymal stem cells contribute formation due their unique cargo proteins, miRNAs, cytokines. Conversely, secreted osteoclasts immune promote resorption inhibit formation. Furthermore, use as therapeutic modalities or biomaterials for diagnosing managing osteoporosis promising. Here, we review current understanding impact homeostasis, including classification biogenesis regulatory present an overview latest research progress treating using EVs. Finally, discuss challenges prospects translational

Язык: Английский

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Research Progress in Hydrogels for Cartilage Organoids

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(22)

Опубликована: Май 21, 2024

The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development

Язык: Английский

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MMP13-targeted siRNA-loaded micelles for diagnosis and treatment of posttraumatic osteoarthritis

Bioactive Materials, Год журнала: 2024, Номер 37, С. 378 - 392

Опубликована: Апрель 23, 2024

Posttraumatic osteoarthritis (PTOA) patients are often diagnosed by X-ray imaging at a middle-late stage when drug interventions less effective. Early PTOA is characterized overexpressed matrix metalloprotease 13 (MMP13). Herein, we constructed an integrated diagnosis and treatment micelle modified with MMP13 enzyme-detachable, cyanine 5 (Cy5)-containing PEG, black hole quencher-3 (BHQ3), cRGD ligands loaded siRNA silencing (siM13), namely ERMs@siM13. ERMs@siM13 could be cleaved in the diseased cartilage tissues to detach PEG shell, causing exposure. Accordingly, ligand exposure promoted uptake chondrocytes binding cell surface αvβ3 integrin, increasing intracellular siM13 delivery for on-demand downregulation. Meanwhile, Cy5 fluorescence was restored detaching from BHQ3-containing micelle, precisely reflecting state. In particular, intensity of generated that hinged on levels reflect severity, enabling physicians adjust therapeutic regimen. Finally, murine model, diagnose early-stage PTOA, perform timely interventions, monitor OA progression level during through real-time detection MMP13. Therefore, represents appealing approach theranostics.

Язык: Английский

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Neddylation suppression by a macrophage membrane-coated nanoparticle promotes dual immunomodulatory repair of diabetic wounds

Bioactive Materials, Год журнала: 2024, Номер 34, С. 366 - 380

Опубликована: Янв. 6, 2024

Oxidative stress, infection, and vasculopathy caused by hyperglycemia are the main barriers for rapid repair of foot ulcers in patients with diabetes mellitus (DM). In recent times, discovery neddylation, a new type post-translational modification, has been found to regulate various crucial biological processes including cell metabolism cycle. Nevertheless, its capacity control healing wounds diabetic remains unknown. This study shows that MLN49224, compound inhibits neddylation at low concentrations, enhances inhibiting polarization M1 macrophages reducing secretion inflammatory factors. Moreover, it concurrently stimulates growth, movement, formation blood vessel endothelial cells, leading expedited individuals diabetes. The drug is loaded into biomimetic macrophage-membrane-coated PLGA nanoparticles (M-NPs/MLN4924). membrane shields from being eliminated reticuloendothelial system counteracts proinflammatory cytokines alleviate inflammation surrounding area. extended discharge MLN4924 M-NPs/MLN4924 growth cells tubes, along towards anti-inflammatory M2 phenotype. By loading hydrogel, final formulation able meaningfully wound, suggesting promising engineered nanoplatform tissue engineering.

Язык: Английский

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Engineered extracellular vesicles with sequential cell recruitment and osteogenic functions to effectively promote senescent bone repair

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 12, 2025

Senescent mandibular bone repair poses a formidable challenge without completely satisfactory strategy. Endogenous cell recruitment and osteogenic differentiation are two sequential stages in regeneration, disruptions these processes present significant obstacles to senescent repair. To address issues, engineered extracellular vesicles (EV) with stem functions were developed. This study demonstrated that Apt19s-engineered (Apt19s-EV) recognize recruit marrow mesenchymal cells derived from old rats (O-BMSCs) specifically effectively. MiR-376b-5p, identified by RNA sequencing transfection, was significantly decreased O-BMSCs, it selected construct miR-376b-5p-engineered (376b-EV). 376b-EV could promote osteogenesis alleviate senescence of O-BMSCs targeting Camsap1. combine the advantages Apt19s miR-376b-5p, dual (Apt-376b-EV) comprising both miR-376b-5p modifications constructed. further validate its function, Gelatin methacryloyl (GelMA) hydrogel used as carrier Apt-376b-EV@GelMA delivery system. The vitro results have sequentially. Notably, vivo also showed sequentially endogenous enhance new formation fracture critical-sized defect models. In summary, vesicles, Apt-376b-EV, offer an appealing solution for recruiting promoting microenvironment, which may broaden clinical applications EV provide valuable strategies treating bone-related diseases future work.

Язык: Английский

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Exosomes from young plasma alleviate osteoporosis through miR-217-5p-regulated osteogenesis of bone marrow mesenchymal stem cell

Composites Part B Engineering, Год журнала: 2024, Номер 276, С. 111358 - 111358

Опубликована: Март 4, 2024

Язык: Английский

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