Plant‐derived extracellular vesicles: Recent advancements and current challenges on their use for biomedical applications

Journal of Extracellular Vesicles, Год журнала: 2022, Номер 11(12)

Опубликована: Дек. 1, 2022

Abstract Extracellular vesicles (EVs) represent a diverse class of lipid bilayer membrane released by both animal and plant cells. These ubiquitous are involved in intercellular communication transport various biological cargos, including proteins, lipids, nucleic acids. In recent years, interest plant‐derived EVs has increased tremendously, as they serve scalable sustainable alternative to derived from mammalian sources. vitro vivo findings have demonstrated that these (PDVs) possess intrinsic therapeutic activities can potentially treat diseases improve human health. addition, PDVs also act efficient biocompatible drug carriers. While preclinical studies shown promising results, there still several challenges knowledge gaps be addressed for the successful translation into clinical applications, especially view lack standardised protocols material handling PDV isolation This review provides readers with quick overview current understanding research on PDVs, critically analysing highlighting immense potential novel therapeutics diseases. It is expected this work will guide scientists address currently associated promote new advances plant‐based solutions.

Язык: Английский

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Plant exosome nanovesicles (PENs): green delivery platforms

Materials Horizons, Год журнала: 2023, Номер 10(10), С. 3879 - 3894

Опубликована: Янв. 1, 2023

Structure of plant-derived vesicles and their bioactive applications. Created with BioRender.com

Язык: Английский

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Therapeutic potential of plant-derived extracellular vesicles as nanocarriers for exogenous miRNAs

Pharmacological Research, Год журнала: 2023, Номер 198, С. 106999 - 106999

Опубликована: Ноя. 19, 2023

Cell-to-cell communication strategies include extracellular vesicles (EVs) in plants and animals. The bioactive molecules a diet rich vegetables fruits are associated with disease-preventive effects. Plant-derived EVs (PDEVs) biogenetically morphologically comparable to mammalian transport molecules, including miRNAs. However, the biological functions of PDEVs not fully understood, standard isolation protocols lacking. Here, were isolated from four foods combination ultracentrifugation size exclusion chromatography, evaluated as vehicles for enhanced synthetic In addition, role food-derived carriers dietary (poly)phenols other secondary metabolites was investigated. broccoli, pomegranate, apple, orange efficiently characterized. all sources, 4 miRNA families present tissues EVs. miRNAs broccoli fruit-derived showed reduced RNase degradation ferried inside exposed cells. transfected ath-miR159a, ath-miR162a-3p, ath-miR166b-3p, ath-miR396b-5p toxic effects on human cells, did natural alone. trace amounts phytochemicals, flavonoids, anthocyanidins, phenolic acids, or glucosinolates. Thus, can act nanocarriers functional that could be used RNA-based therapy.

Язык: Английский

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Turmeric‐Derived Nanoparticles Functionalized Aerogel Regulates Multicellular Networks to Promote Diabetic Wound Healing

Advanced Science, Год журнала: 2024, Номер 11(18)

Опубликована: Март 5, 2024

Abstract Regulation of excessive inflammation and impaired cell proliferation is crucial for healing diabetic wounds. Although plant‐to‐mammalian regulation offers effective approaches chronic wound management, the development a potent plant‐based therapeutic presents challenges. This study aims to validate efficacy turmeric‐derived nanoparticles (TDNPs) loaded with natural bioactive compounds. TDNPs can alleviate oxidative stress, promote fibroblast migration, reprogram macrophage polarization. Restoration fibroblast–macrophage communication network by stimulates cellular regeneration, in turn enhancing healing. To address are an ultralight‐weight, high swelling ratio, breathable aerogel (AG) constructed cellulose nanofibers sodium alginate backbones obtain TDNPs@AG (TAG). TAG features shape‐customized accessibility, water‐adaptable tissue adhesiveness, capacity sustained release TDNPs, exhibiting outstanding performance facilitating vivo highlights potential regenerative medicine their applicability as promising solution clinical settings.

Язык: Английский

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Research status and challenges of plant-derived exosome-like nanoparticles

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 174, С. 116543 - 116543

Опубликована: Апрель 11, 2024

In recent years, there has been an increasing number of related studies on exosomes. Most have focused exosomes derived from mammals, confirming the important role that play in cell communication. Plants, as a natural ingredient, plant-derived confirmed to similar structures and functions mammalian-derived Plant-derived exosome-like nanoparticles (PELNs) are lipid bilayer membrane nanovesicles containing bioactive constituents such miRNA, mRNA, protein, lipids obtained plant cells, can participate intercellular communication mediate transboundary communication, high bioavailability low immunogenicity, relatively safe, shown maintaining homeostasis preventing, treating variety diseases. this review, we describe biogenesis, isolation purification methods, structural composition, stability, safety, function PELNs challenges. The anti-inflammatory, antioxidant, antitumor drug delivery mainly described, status research exosome Chinese herbal medicines is outlined. Overall, summarized importance latest results field provided theoretical basis for future clinical application PELNs.

Язык: Английский

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Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy

Bioactive Materials, Год журнала: 2024, Номер 38, С. 1 - 30

Опубликована: Апрель 23, 2024

Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.

Язык: Английский

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Exosome-like nanoparticles derived from fruits, vegetables, and herbs: innovative strategies of therapeutic and drug delivery

Theranostics, Год журнала: 2024, Номер 14(12), С. 4598 - 4621

Опубликована: Янв. 1, 2024

Over the past ten years, significant advancements have been made in exploring plant-derived exosome-like nanoparticles (PELNs) for disease therapeutics and drug delivery. PELNs, as inherent nanoscale particles comprised of proteins, lipids, nucleic acids, secondary metabolites, exhibit capacity cellular uptake by human cells. This intercellular interaction transcends biological boundaries, effectively influencing functions animals. PELNs outstanding biocompatibility, low immunogenicity, enhanced safety, environmentally friendly sustainability. article summarized preparation methods characteristics PELNs. It provided a systematic review varied roles derived from fruits, vegetables, herbs The challenges their production application were discussed, future prospects this rapidly evolving field explored.

Язык: Английский

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Bioactive compounds and biological functions of medicinal plant-derived extracellular vesicles

Pharmacological Research, Год журнала: 2024, Номер 200, С. 107062 - 107062

Опубликована: Янв. 9, 2024

Extracellular vesicles (EVs) are tiny lipid bilayer-enclosed membrane particles released from a variety of cell types into the surrounding environment. These EVs have massive participated in cell-to-cell communication and interspecies communication. In recent years, plant-derived extracellular (PDEVs) "exosome-like" populations found distinct plants attracted widespread attention. Especially, research on medicinal (MPDEVs) increasing, which considered kind promising natural compound. This review summarizes current knowledge MPDEVs terms bioactive compounds, including small RNA, protein, lipid, metabolite, been surface and/or lumen MPDEVs. Moreover, both vitro vivo experiments shown that exert broad biomedical functions, such as anti-inflammatory, anticancer, antioxidant, modulate microbiota, etc. may be better substitute than animal-derived (ADEVs) because safety biocompatibility future.

Язык: Английский

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Gut‐liver axis: Potential mechanisms of action of food‐derived extracellular vesicles

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(6)

Опубликована: Июнь 1, 2024

Abstract Food‐derived extracellular vesicles (FEVs) are nanoscale membrane obtained from dietary materials such as breast milk, plants and probiotics. Distinct other EVs, FEVs can survive the harsh degrading conditions in gastrointestinal tract reach intestines. This unique feature allows to be promising prebiotics health oral nanomedicine for gut disorders, inflammatory bowel disease. Interestingly, therapeutic effects of have recently also been observed non‐gastrointestinal diseases. However, mechanisms remain unclear or even mysterious. It is speculated that orally administered could enter bloodstream, remote organs, thus exert therein. emerging evidence suggests amount reaching organs beyond marginal may insufficient account significant achieved regarding diseases involving liver. Thus, we herein propose primarily act locally intestine by modulating intestinal microenvironments barrier integrity microbiota, thereby eliciting impact remotely on liver via gut‐liver axis. Likewise, drugs delivered system through As main metabolic hub, microenvironment implicated In fact, many patients with non‐alcoholic fatty disease, obesity, diabetes cardiovascular disease suffer a leaky dysbiosis. this review, provide an overview recent progress discuss their biomedical applications agents drug delivery systems, highlighting pivotal role axis action treatment disorders

Язык: Английский

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Plant-Derived Extracellular Vesicles: A New Revolutionization of Modern Healthy Diets and Biomedical Applications

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(6), С. 2853 - 2878

Опубликована: Фев. 1, 2024

Plant-derived extracellular vesicles (PDEVs) have recently emerged as a promising area of research due to their potential health benefits and biomedical applications. Produced by various plant species, these EVs contain diverse bioactive molecules, including proteins, lipids, nucleic acids. Increasing in vitro vivo studies shown that PDEVs inherent pharmacological activities affect cellular processes, exerting anti-inflammatory, antioxidant, anticancer activities, which can potentially contribute disease therapy improve human health. Additionally, efficient biocompatible drug delivery vehicles treating diseases. However, while serve rising star modern healthy diets applications, further is needed address underlying knowledge gaps, especially the lack standardized protocols for isolation, identification, large-scale production. Furthermore, safety efficacy clinical applications must be thoroughly evaluated. In this review, we concisely discuss current PDEV field, characteristics, isolation methods, provide an overview state research. Finally, challenges regarding prospective issues PDEVs. This review expected new insights into vegetables fruits, inspiring advances natural food-based science technology.

Язык: Английский

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