TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 167, С. 117269 - 117269
Опубликована: Сен. 7, 2023
Язык: Английский
Analytical Chemistry, Год журнала: 2024, Номер 96(6), С. 2676 - 2683
Опубликована: Янв. 30, 2024
Sepsis is an extremely dangerous medical condition that emanates from the body's response to a pre-existing infection. Early detection of sepsis-inducing bacterial infections can greatly enhance treatment process and potentially prevent onset sepsis. However, current point-of-care (POC) sensors are often complex costly or lack ideal sensitivity for effective detection. Therefore, it crucial develop rapid sensitive biosensors on-site bacteria. Herein, we developed graphene oxide CRISPR-Cas12a (GO-CRISPR) biosensor bacteria in human serum. In this strategy, single-stranded (ssDNA) FAM probes were quenched with single-layer (GO). Target-activated Cas12a trans-cleavage was utilized degradation ssDNA probes, detaching short GO recovering fluorescent signals. Under optimal conditions, employed our GO-CRISPR system Salmonella Typhimurium (S. Typhimurium) as low 3 × 103 CFU/mL serum, well good specificity toward other competing addition, exhibited excellent S. spiked The offers superior rapidity has potential early resource-limited settings, expediting patients at risk
Язык: Английский
Процитировано
20
Biosensors and Bioelectronics, Год журнала: 2022, Номер 212, С. 114428 - 114428
Опубликована: Май 27, 2022
Язык: Английский
Процитировано
59
Analytical Science Advances, Год журнала: 2022, Номер 3(3-4), С. 113 - 145
Опубликована: Март 23, 2022
As the human population grows, anthropogenic impacts from various agricultural and industrial processes produce unwanted contaminants in environment. The accurate, sensitive rapid detection of such is vital for health safety. Surface-enhanced Raman spectroscopy (SERS) a valuable analytical tool with wide applications environmental contaminant monitoring. aim this review to summarize recent advancements within SERS research as it applies detection, focus on published or accessible January 2021 through December including early-access publications. Our goal provide breadth information that can be used background knowledge field, well inform encourage further development techniques protecting quality Specifically, we highlight characteristics effective nanosubstrates, explore methods inorganic, organic, biological heavy metals, pharmaceuticals, plastic particles, synthetic dyes, pesticides, viruses, bacteria mycotoxins. We also discuss current limitations technologies propose several avenues future investigation. researchers fill identified gaps so implemented real-world environment more effectively efficiently, ultimately providing reliable timely data help make science-based strategies policies protect safety public health.
Язык: Английский
Процитировано
58
Microorganisms, Год журнала: 2022, Номер 10(6), С. 1193 - 1193
Опубликована: Июнь 10, 2022
COVID-19 emerged in late 2019 China and quickly spread across the globe, causing over 521 million cases of infection 6.26 deaths to date. After 2 years, numerous advances have been made. First all, preventive vaccine, which has implemented record time, is effective more than 95% cases. Additionally, diagnostic field, there are molecular antigenic kits that equipped with high sensitivity specificity. Real Time-PCR-based assays for detection viral RNA currently considered gold-standard method SARS-CoV-2 diagnosis can be used efficiently on pooled nasopharyngeal, or oropharyngeal samples widespread screening. Moreover, additional, advanced methods such as droplet-digital PCR (ddPCR), clustered regularly interspaced short palindromic repeats (CRISPR) next-generation sequencing (NGS), under development detect RNA. However, number subjects infected continuously increases globally, health care systems being placed increased stress. Thus, clinical laboratory plays an important role, helping select especially asymptomatic individuals who actively carrying live replicating virus, fast non-invasive technologies. Recent strategies, other methods, adopted either antigens, i.e., antigen-based immunoassays, human anti-SARS-CoV-2 antibodies, antibody-based nasal swabs, well blood saliva samples. role mucosal sIgAs, essential control viruses entering body through surfaces, remains elucidated, particular immune response counteracting infection, primarily at site(s) virus entry appears promising.
Язык: Английский
Процитировано
53
Frontiers in Microbiology, Год журнала: 2022, Номер 13
Опубликована: Дек. 8, 2022
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a natural adaptive immune of prokaryotes. CRISPR-Cas currently divided into two classes and six types: types I, III, IV in class 1 systems II, V, VI 2 systems. Among the type systems, CRISPR/Cas13a has been most widely characterized for its application molecular diagnostics, gene therapy, editing, RNA imaging. Moreover, because trans-cleavage activity Cas13a high specificity CRISPR RNA, enormous potential field diagnostics. Herein, we summarize applications detection pathogens, including viruses, bacteria, parasites, chlamydia, fungus; biomarkers, such as microRNAs, lncRNAs, circRNAs; some non-nucleic acid targets, proteins, ions, methyl groups. Meanwhile, highlight working principles novel Cas13a-based methods, Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK) improved versions, nucleic amplification-free biosensors, biosensors target detection. Finally, focus on issues that need to be solved development prospects system.
Язык: Английский
Процитировано
52
Biomedicine & Pharmacotherapy, Год журнала: 2022, Номер 153, С. 113538 - 113538
Опубликована: Авг. 12, 2022
The World Health Organizations declaration of the COVID-19 pandemic was a milestone for scientific community. high transmission rate and huge number deaths, along with lack knowledge about virus evolution disease, stimulated relentless search diagnostic tests, treatments, vaccines. main challenges were differential diagnosis development specific, rapid, sensitive tests that could reach all people. RT-PCR remains gold standard diagnosing COVID-19. However, new methods, such as other molecular techniques immunoassays emerged. Also, need accessible quick results boosted point care (POCT) are fast, automated, precision accuracy. This assay reduces dependence on laboratory conditions mass testing population, dispersing pressure regarding screening detection. review summarizes advances in field since started, emphasizing various detecting We reviewed existing well POCT under development, starting detection, but also exploring nucleic acid techniques, digital PCR, loop-mediated isothermal amplification-based (RT-LAMP), clustered regularly interspaced short palindromic repeats (CRISPR), next-generation sequencing (NGS), immunoassay nanoparticle-based biosensors, developed portable instruments rapid
Язык: Английский
Процитировано
48
Trends in biotechnology, Год журнала: 2022, Номер 41(4), С. 557 - 574
Опубликована: Авг. 18, 2022
Язык: Английский
Процитировано
47
ACS Sensors, Год журнала: 2023, Номер 8(7), С. 2852 - 2858
Опубликована: Июль 4, 2023
Rapid and accurate detection of biomarkers was very important for early screening treatment diseases. Herein, a sensitive amplification-free electrochemiluminescence (ECL) biosensor based on CRISPR/Cas12a DNA tetrahedron nanostructures (TDNs) constructed. Briefly, 3D TDN self-assembled the Au nanoparticle-deposited glassy carbon electrode surface to construct biosensing interface. The presence target would activate trans-cleavage activity Cas12a-crRNA duplex cleave single-stranded signal probe vertex TDN, causing Ru(bpy)32+ fall from weakened ECL signal. Thus, system transduced change concentration into an enabling HPV-16. specific recognition HPV-16 made have good selectivity, while TDN-modified sensing interface could reduce cleaving steric resistance improve performance CRISPR/Cas12a. In addition, pretreated complete sample within 100 min with limit 8.86 fM, indicating that developed possesses potential application prospect fast nucleic acid detection.
Язык: Английский
Процитировано
43
Frontiers in Microbiology, Год журнала: 2023, Номер 14
Опубликована: Ноя. 23, 2023
The World Health Organization (WHO) estimated that pathogens like Escherichia coli, primarily linked to food and water contamination, are associated with 485,000 deaths from diarrheal diseases annually, translating a staggering worldwide economic loss of nearly 12 billion USD per annum. International organizations the WHO United Nations Children’s Fund (UNICEF) have established related guidelines criteria for pathogenic detection technologies driving search innovative efficient methods. This comprehensive review examines trajectory waterborne bacteria traditional techniques, i.e., culture-based methods, current methods including various forms polymerase chain reaction (PCR) techniques [qualitative real-time PCR, digital ELISA, loop-mediated isothermal amplification, next-generation sequencing (NGS)] emerging biosensors artificial intelligence (AI). scope paper focuses on recognized as human pathogens, posing tangible threats public health through waterborne. techniques’ merits, constraints, research gaps future perspectives critically discussed. Advancements in droplet NGS significantly improved sensitivity specificity, revolutionizing pathogen detection. Additionally, integration (AI) these has enhanced accuracy, enabling analysis large datasets. Molecular-based show promise quality monitoring, especially resource-constrained settings, but on-site practical implementation remains challenge. pairwise comparison metrics used this also offer valuable insights into quick evaluation advantages, limitations focusing their applicability field settings timely analyses. Future efforts should focus developing robust, cost-effective user-friendly routine ultimately safeguarding global supplies health, AI data playing crucial role advancing safer environment.
Язык: Английский
Процитировано
43
Biosensors and Bioelectronics, Год журнала: 2023, Номер 228, С. 115179 - 115179
Опубликована: Март 1, 2023
Язык: Английский
Процитировано
41