Clade-specific extracellular vesicles from Akkermansia muciniphila mediate competitive colonization via direct inhibition and immune stimulation

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 19, 2025

Akkermansia muciniphila, a promising candidate for next-generation probiotics, exhibits significant genomic diversity, classified into several distinct clades (AmI to AmIV). Notably, single clade tends predominate within individual hosts, with co-occurrence of different being rare. The mechanisms driving such clade-specific exclusion remain unclear. Here, we show that extracellular vesicles (EVs) derived from AmII inhibit the growth I (AmI), conferring competitive advantage AmII. Moreover, observe immunoglobulin A (IgA) responses, where IgAs, induced by EVs AmII, facilitate niche occupancy and AmI. These findings provide insights dynamics A. muciniphila suggest future personalized microbiome interventions could be optimized considering composition in hosts.

Язык: Английский

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Isoxanthohumol improves obesity and glucose metabolism via inhibiting intestinal lipid absorption with a bloom of Akkermansia muciniphila in mice

Molecular Metabolism, Год журнала: 2023, Номер 77, С. 101797 - 101797

Опубликована: Сен. 12, 2023

Polyphenols have health-promoting effects, such as improving insulin resistance. Isoxanthohumol (IX), a prenylated flavonoid found in beer hops, has been suggested to reduce obesity and resistance; however, the mechanism remains unknown.High-fat diet-fed mice were administered IX. We analyzed glucose metabolism, gene expression profiles histology of liver, epididymal adipose tissue colon. Lipase activity, fecal lipid plasma metabolomic analysis assessed. Fecal 16s rRNA sequencing was obtained selected bacterial species used for vitro studies. microbiota transplantation monocolonization conducted antibiotic-treated or germ-free (GF) mice.The administration IX lowered weight gain, decreased steatohepatitis improved metabolism. Mechanistically, inhibited pancreatic lipase activity absorption by decreasing fatty acid transporter CD36 small intestine, which confirmed increased excretion feces. markers intestinal barrier function, including thickening mucin layer increasing caludin-1, tight-junction related protein In contrast, effects nullified antibiotics. As revealed using 16S sequencing, microbial community structure changed with significant increase abundance Akkermansia muciniphila IX-treated group. An anaerobic chamber study showed that selectively promoted growth A. while exhibiting antimicrobial against some Bacteroides Clostridium species. To further explore direct effect on we monocolonized either thetaiotaomicron GF mice. jejunum levels multiple classes acids determined analysis.Our demonstrated prevents enhances metabolism inhibiting dietary fat absorption. This is linked suppressing shifts composition, notably an muciniphila. These highlight new treatment strategies preventing metabolic syndrome boosting gut food components.

Язык: Английский

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Multi-strain probiotics alleviate loperamide-induced constipation by adjusting the microbiome, serotonin, and short-chain fatty acids in rats

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Июнь 2, 2023

Constipation is one of the most common gastrointestinal (GI) disorders worldwide. The use probiotics to improve constipation well known. In this study, effect on loperamide-induced by intragastric administration Consti-Biome mixed with SynBalance® SmilinGut (Lactobacillus plantarum PBS067, Lactobacillus rhamnosus LRH020, Bifidobacterium animalis subsp. lactis BL050; Roelmi HPC), L. UALp-05 (Chr. Hansen), acidophilus DDS-1 and Streptococcus thermophilus CKDB027 (Chong Kun Dang Bio) rats was evaluated. To induce constipation, 5 mg/kg loperamide intraperitoneally administered twice a day for 7 days all groups except normal control group. After inducing Dulcolax-S tablets multi-strain were orally once 14 days. 0.5 mL at concentrations 2 × 108 CFU/mL (G1), 109 (G2), 1010 (G3). Compared group (LOP), not only significantly increased number fecal pellets but also improved GI transit rate. mRNA expression levels serotonin- mucin-related genes in colons that treated compared LOP addition, an increase serotonin observed colon. cecum metabolites showed different pattern between probiotics-treated group, short-chain fatty acids probiotic-treated groups. abundances phylum Verrucomicrobia, family Erysipelotrichaceae genus Akkermansia samples Therefore, used experiment thought help alleviate LOP-induced altering acids, serotonin, mucin through improvement intestinal microflora.

Язык: Английский

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Heat-killed Akkermansia muciniphila ameliorates allergic airway inflammation in mice

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Май 9, 2024

Allergic asthma (AA) is a common inflammatory airway disease characterized by increased hyper-responsiveness (AHR), inflammation, and remodeling. Akkermansia muciniphila strictly anaerobic bacterium residing in the gut promising next-generation probiotic to improve metabolic syndrome. A recent study suggested beneficial effect of live A. on allergic inflammation (AAI) mice. However, whether heat-killed form can AAI requires further investigation. Mice sensitized challenged with house dust mites (HDM) develop AA hallmarks including cell infiltration, goblet hyperplasia, subepithelial collagen deposition lungs. These phenomena were reversed oral administration strain EB-AMDK19 (AMDK19-HK) isolated from feces healthy Koreans. Furthermore, AMDK19-HK diminished HDM-induced AHR inhaled methacholine, lung mast accumulation, serum HDM-specific IgE levels. It also led overall suppression IL-4, IL-13, eotaxin production bronchoalveolar lavage fluids, Il4 , Il5 Il13 Ccl17 gene expression tissues. Moreover, suppressed Th2-associated cytokine splenocytes HDM-sensitized mice vitro . Additionally, combination 16S rRNA sequencing short-chain fatty acid (SCFA) analysis cecal samples revealed that modulated relative abundance circulating SCFA-associated genera, positive correlation Lachnospiraceae_ NK4A136_group negative Lachnoclostridium significantly SCFA concentrations. Finally, improved intestinal mucosal barrier function. results suggest ameliorates suppressing Th2-mediated immune responses could have protective against development.

Язык: Английский

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A history of repeated antibiotic usage leads to microbiota-dependent mucus defects

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Июль 21, 2024

Recent evidence indicates that repeated antibiotic usage lowers microbial diversity and ultimately changes the gut microbiota community. However, physiological effects of - but not recent on microbiota-mediated mucosal barrier function are largely unknown. By selecting human individuals from deeply phenotyped Estonian Microbiome Cohort (EstMB), we here utilized human-to-mouse fecal transplantation to explore long-term impacts use intestinal mucus function. While a healthy layer protects epithelium against infection inflammation, using

Язык: Английский

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Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the “microbiota-gut-joint” axis

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Апрель 8, 2024

Abstract Osteoarthritis (OA) is a common degenerative joint disease that can cause severe pain, motor dysfunction, and even disability. A growing body of research indicates gut microbiota their associated metabolites are key players in maintaining bone health the progression OA. Short-chain fatty acids (SCFAs) series active widely participate homeostasis. Gold nanoparticles (GNPs) with outstanding anti-bacterial anti-inflammatory properties, have been demonstrated to ameliorate excessive loss during osteoporosis (OP) rheumatoid arthritis (RA). However, protective effects GNPs on OA not clear. Here, we observed significantly alleviated anterior cruciate ligament transection (ACLT)-induced microbiota-dependent manner. 16S rDNA gene sequencing showed changed microbial diversity structure, which manifested as an increase abundance Akkermansia Lactobacillus . Additionally, increased levels SCFAs (such butyric acid), could improved destruction by reducing inflammatory response. Notably, modulated dynamic balance M1/M2 macrophages, serum cytokines such IL-10. To sum up, our study indicated exhibited anti-osteoarthritis via modulating interaction “microbiota-gut-joint” axis, might provide promising therapeutic strategies for

Язык: Английский

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YTHDF1 Attenuates TBI-Induced Brain-Gut Axis Dysfunction in Mice

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 4240 - 4240

Опубликована: Фев. 20, 2023

The brain-gut axis (BGA) is a significant bidirectional communication pathway between the brain and gut. Traumatic injury (TBI) induced neurotoxicity neuroinflammation can affect gut functions through BGA. N6-methyladenosine (m6A), as most popular posttranscriptional modification of eukaryotic mRNA, has recently been identified playing important roles in both However, whether m6A RNA methylation involved TBI-induced BGA dysfunction not clear. Here, we showed that YTHDF1 knockout reduced histopathological lesions decreased levels apoptosis, inflammation, oedema proteins tissues mice after TBI. We also found improved fungal mycobiome abundance probiotic (particularly Akkermansia) colonization at 3 days post-CCI. Then, differentially expressed genes (DEGs) cortex YTHDF1-knockout WT mice. These were primarily enriched regulation neurotransmitter-related neuronal signalling pathways, inflammatory apoptotic pathways. This study reveals ITGA6-mediated cell adhesion molecule may be key feature dysfunction. Our results suggest could attenuate

Язык: Английский

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Possible role of nutrition in the prevention of inflammatory bowel disease–related colorectal cancer: A focus on human studies

Nutrition, Год журнала: 2023, Номер 110, С. 111980 - 111980

Опубликована: Фев. 3, 2023

Patients with inflammatory bowel disease (IBD) are at substantially high risk for colorectal cancer (CRC). IBD-associated CRC accounts roughly 10% to 15% of the annual mortality in patients IBD. IBD-related also affects younger compared sporadic CRC, a 5-y survival rate 50%. Regardless medical therapies, persistent state characterizing IBD raises precancerous changes and additional input from several elements, including genetic environmental factors, comorbidities, intestinal barrier dysfunction, gut microbiota modifications. It is well known that nutritional habits dietary bioactive compounds can influence inflammation, microbiome abundance composition, oxidative stress balance, permeability. Additionally, recent years, results broad epidemiologic experimental studies have associated certain foods or patterns neoplasia. The present study aimed review possible role nutrition preventing focusing specifically on human studies. emerges interventions based healthy, nutrient-dense characterized by intake fiber, vegetables, fruit, ω-3 polyunsaturated fatty acids, low amount animal proteins, processed foods, alcohol, combined probiotic supplementation potential reducing IBD-activity through different mechanisms, suggesting targeted may represent novel promising approach prevention management CRC.

Язык: Английский

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The interplay between the microbiota, diet and T regulatory cells in the preservation of the gut barrier in inflammatory bowel disease

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Дек. 1, 2023

Inflammatory bowel disease (IBD) is becoming more common in the Western world due to changes diet-related microbial dysbiosis, genetics and lifestyle. Incidences of gut permeability can predate IBD continued barrier disruptions increase exposure bacterial antigens immune system thereby perpetuating chronic inflammation. Currently, most approved therapies target individual pro-inflammatory cytokines pathways. However, they fail approximately 50% patients their inability overcome redundant pro inflammatory responses. There increasing interest therapeutic potential T regulatory cells (Tregs) conditions widespread capability dampen inflammation, promote tolerance intestinal bacteria, facilitate healing mucosal ability be engineered for targeted therapy. Intestinal Treg populations are inherently shaped by dietary molecules microbiota-derived metabolites. Thus, understanding how these influence Treg-mediated preservation will provide insights into tolerance-mediated homeostasis. This review comprehensively explores interplay between diet, microbiota, influencing function attenuate progression colitis.

Язык: Английский

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Distinct protective effects of a novel Akkermansia sp. BCRC 18949 against DSS-Induced colitis in mice

Journal of Functional Foods, Год журнала: 2024, Номер 115, С. 106110 - 106110

Опубликована: Март 9, 2024

The emergence of next-generation probiotics (NGPs) has illuminated new possibilities for improving various diseases through food-grade supplements. In this study, a gut bacterial species, designated as Akkermansia sp. BCRC 18949, was isolated from the feces healthy Taiwanese adult. phylogenetic tree and whole genome sequencing analyses confirmed that human commensal is novel species belonging to genus Akkermansia. Our results reveals 18949 exhibits distinct mucin utilization abilities compared ATCC BAA-835. Importantly, shows enhanced protection against DSS-induced colitis, while both contribute differentially restoring barrier integrity in DSS-treated mice. Furthermore, significantly restores expression colonic rhythmic genes, suggesting its potential role maintaining circadian rhythm colon during colitis. findings provide valuable insights into functional diversity their therapeutic applications

Язык: Английский

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