Molecular Medicine,
Год журнала:
2022,
Номер
28(1)
Опубликована: Июнь 17, 2022
At
present,
the
molecular
mechanisms
underlying
inflammation
remain
unclear.
In
recent
years,
research
on
has
focused
stimulating
cell
by
using
exogenous
pro-inflammatory
substances
such
as
lipopolysaccharide
(LPS)
or
inflammatory
factors.
To
investigate
mechanism
of
from
a
new
perspective,
we
designed
nucleic
acid
nanoflowers
(NFs)
complex
to
directly
activate
genes
study
response
without
need
for
external
microbial
factors
trigger
an
response.
An
RNAa-type
target
gene-activated
NFs
was
designed.
Human
umbilical
vein
endothelial
cells
(HUVECs)
were
transfected
with
carrying
small
activating
RNA
(saRNAs)
co-activate
microRNA
(miR)-155
and
SHIP1
genes.
After
activation
(RNAa)-type
transferred
into
HUVECs,
expression
miR-155
cancer-related
increased,
anti-inflammatory
reduced,
proliferation
migration
promoted.
IL-1β
protein
levels
decreased
downregulated.
When
its
both
activated,
unaltered,
maintaining
homeostasis.
This
points
towards
overexpression
can
inflammation,
that
act
switch
role
in
development
inflammation.
Translational Oncology,
Год журнала:
2023,
Номер
31, С. 101653 - 101653
Опубликована: Март 11, 2023
Triple
negative
breast
cancer
(TNBC)
is
known
as
hot
immunogenic
tumor.
Yet,
it
one
of
the
most
aggressive
BC
subtypes.
TNBC
evolve
several
tactics
to
evade
immune
surveillance
phenomena,
which
shedding
natural
killer
(NK)
cells
activating
ligands
such
MICA/B
and/or
by
inducing
expression
checkpoints
PD-L1
and
B7-H4.
MALAT-1
an
oncogenic
lncRNA.
profile
not
well
investigated.
The
study
aims
at
exploring
role
in
patients
cell
lines
identify
its
molecular
mechanism
altering
both
innate
adaptive
present
tumor
microenvironment
(n
=
35)
were
recruited.
Primary
NK
cytotoxic
T
lymphocytes
isolated
from
normal
individuals
using
selection
method.
MDA-MB-231
cultured
transfected
oligonucleotides
lipofection
technique.
Screening
ncRNAs
was
performed
q-RT-PCR.
Immunological
functional
analysis
experiments
upon
co-culturing
primary
LDH
assay.
Bioinformatics
potential
microRNAs
targeted
MALAT-1.
significantly
upregulated
patinets
with
a
profound
compared
their
counterparts.
Correlation
revealed
positive
correlation
between
MALAT-1,
size
lymph
node
metastasis.
Knocking
down
resulted
significant
induction
MICA/B,
repression
B7-H4
levels.
Enhancement
activity
co-cultured
CD8+
siRNAs
cells.
In
silico
that
miR-34a
miR-17–5p
are
targets
MALAT-1;
accordingly,
they
found
be
downregulated
patients.
Forcing
Ectopic
repressed
checkpoints.
Validations
MALAT-1/miR-34a"
"MALAT-1/miR-17–5p
axes
series
co-transfections
assessment
This
proposes
novel
epigenetic
alteration
exerted
mainly
mediates
suppression
events
partially
via
targeting
miR-34a/MICA/B
miR-175p/PD-L1/B7-H4
lines.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(4), С. 3694 - 3694
Опубликована: Фев. 12, 2023
MicroRNAs
are
non-coding
RNAs
that
serve
as
regulatory
molecules
in
a
variety
of
pathways
such
inflammation,
metabolism,
homeostasis,
cell
machinery,
and
development.
With
the
progression
sequencing
methods
modern
bioinformatics
tools,
novel
roles
microRNAs
mechanisms
pathophysiological
states
continue
to
expand.
Advances
detection
have
further
enabled
larger
adoption
studies
utilizing
minimal
sample
volumes,
allowing
analysis
low-volume
biofluids,
aqueous
humor
tear
fluid.
The
reported
abundance
extracellular
these
biofluids
has
prompted
explore
their
biomarker
potential.
This
review
compiles
current
literature
reporting
human
fluid
association
with
ocular
diseases
including
dry
eye
disease,
Sjögren’s
syndrome,
keratitis,
vernal
keratoconjunctivitis,
glaucoma,
diabetic
macular
edema,
retinopathy,
well
non-ocular
diseases,
Alzheimer’s
breast
cancer.
We
also
summarize
known
shed
light
on
future
this
field.
Immunity Inflammation and Disease,
Год журнала:
2024,
Номер
12(3)
Опубликована: Март 1, 2024
Abstract
Background
Immunosenescence
is
a
multifactorial
stress
response
to
different
intrinsic
and
extrinsic
insults
that
cause
immune
deterioration
accompanied
by
genomic
or
epigenomic
perturbations.
It
now
widely
recognized
genes
proteins
contributing
in
the
process
of
immunosenescence
are
regulated
various
noncoding
(nc)
RNAs,
including
microRNAs
(miRNAs),
long
ncRNAs,
circular
RNAs.
Aims
This
review
article
aimed
evaluate
regulatore
RNAs
roles
immunosenescence.
Methods
We
analyzed
publications
were
focusing
on
regulatory
several
aspects
Results
In
setting,
ncRNAs
have
been
found
play
at
both
transcriptional
post‐transcriptional
levels.
These
factors
cooperate
regulate
initiation
gene
expression
programs
sustaining
senescence
phenotype
proinflammatory
responses.
Conclusion
complex
with
pivotal
alterations
function
occurring
age.
The
extensive
network
drive
immunosenescence‐related
features
mainly
directed
variety
such
as
miRNAs,
lncRNAs,
circRNAs.
Latest
findings
about
regulation
innate
adaptive
cells
well
their
role
pathways,
provide
better
understanding
Gastric
cancer
(GC)
continues
to
pose
a
major
health
issue
worldwide,
and
diagnoses
made
at
advanced
stages
frequently
result
in
unfavorable
outcomes.
The
metastatic
spread
of
GC
presents
substantial
therapeutic
challenges,
necessitating
deeper
understanding
the
tumor
microenvironment
(TME)
its
interactions
with
cells.
MicroRNAs
(miRNAs)
are
small
non-coding
RNA
molecules
that
regulate
gene
expression
have
been
identified
as
key
factors
metastasis
GC.
This
review
explores
biogenesis
functional
mechanisms
miRNAs,
highlighting
their
dual
roles
oncogenes
suppressors
metastasis.
We
discuss
specific
miRNAs
facilitate
progression
by
influencing
pathways
related
invasion,
angiogenesis,
immune
evasion.
Furthermore,
we
examine
potential
diagnostic
prognostic
biomarkers,
offering
insights
into
utility
timely
identification
tailored
approaches.
By
elucidating
complex
regulatory
networks
involving
this
underscores
significance
both
targets
biomarkers
management
gastric
cancer.
Genes,
Год журнала:
2020,
Номер
11(11), С. 1327 - 1327
Опубликована: Ноя. 10, 2020
Sepsis
is
a
dysregulated
immune
response
disease
affecting
millions
worldwide.
Delayed
diagnosis,
poor
prognosis,
and
heterogeneity
make
its
treatment
ineffective.
miRNAs
are
imposingly
involved
in
personalized
medicine
such
as
therapeutics,
due
to
their
high
sensitivity
accuracy.
Our
study
aimed
reveal
the
biomarkers
that
may
be
sepsis
lung
injury
using
computational
approach
vivo
validation
studies.
A
miRNA
Gene
Expression
Omnibus
(GEO)
dataset
based
on
former
analysis
of
blood
samples
was
used
identify
differentially
expressed
(DEMs)
associated
hub
genes.
Sepsis-associated
genes
from
Comparative
Toxicogenomics
Database
(CTD)
overlapped
with
identified
DEM
targets
were
utilized
for
network
construction.
In
total,
317
found
regulated
by
10
DEMs
(three
upregulated,
namely
miR-4634,
miR-4638-5p,
miR-4769-5p,
seven
downregulated,
miR-4299,
miR-451a,
miR181a-2-3p,
miR-16-5p,
miR-5704,
miR-144-3p,
miR-1290).
Overall
(HIP1,
GJC1,
MDM4,
IL6R,
ERC1)
miR-16-5p
(SYNRG,
TNRC6B,
LAMTOR3)
centrality
measures
(degree,
betweenness,
closeness).
tissue
showed
significantly
downregulated
expression
corroborating
our
findings,
whereas
miR-181a-2-3p
miR-451a
upregulated
contrast
approach.
conclusion,
differential
pattern
reported
this
help
unravel
many
unexplored
regulatory
pathways,
leading
identification
critical
molecular
increased
drug
efficacy
organ
injuries.
Annals of Clinical and Translational Neurology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 29, 2024
Abstract
Objective
Patients
with
ovarian
cancer
(OC)
may
develop
anti‐Yo‐associated
paraneoplastic
cerebellar
degeneration
(PCD)—a
ataxia
associated
tumor‐induced
autoimmunity
against
CDR2
and
CDR2L
proteins.
Dysregulation
of
circulating
exosomal
microRNAs
(miRNAs)
occur
in
OC.
Here,
we
investigated
whether
PCD
is
changes
the
miRNA
profiles
OC
patients.
Methods
Serum
exosomes
were
isolated
from
patients
(
n
=
15
),
14
)
healthy
controls
(HC,
).
Small
RNA
sequencing
was
used
to
identify
differentially
expressed
miRNAs.
Receiver
operating
characteristic
curves
evaluate
biomarker
sensitivity
specificity,
target
prediction
analysis
employed
elucidate
gene
targets.
Results
exhibited
a
distinct
expression
profile.
We
detected
103
miRNAs
compared
without
139
controls.
Particularly
miR‐486‐5p,
miR‐4732‐5p,
miR‐98‐5p
miR‐21‐5p
notable
specificity
for
discriminating
both
showed
that
several
targeted
genes.
Interpretation
Our
results
demonstrate
exhibit
profile
PCD.
Several
diagnostic
potential
hold
relevance
understanding
pathogenesis
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Июнь 2, 2023
Background
Most
commercial
foot-and-mouth
disease
(FMD)
vaccines
have
various
disadvantages,
such
as
low
antibody
titers,
short-lived
effects,
compromised
host
defense,
and
questionable
safety.
Objectives
To
address
these
shortcomings,
we
present
a
novel
FMD
vaccine
containing
Dectin-1
agonist,
β-D-glucan,
an
immunomodulatory
adjuvant.
The
proposed
was
developed
to
effectively
coordinate
innate
adaptive
immunity
for
potent
defense
against
viral
infection.
Methods
We
demonstrated
β-D-glucan
mediated
immune
responses
in
mice
pigs
vitro
vivo
.
expressions
of
pattern
recognition
receptors,
cytokines,
transcription
factors,
co-stimulatory
molecules
were
promoted
via
β-D-glucan.
Results
elicited
robust
cellular
response
early,
mid-,
long-term
immunity.
Moreover,
it
exhibited
by
modulating
host’s
Conclusion
Our
study
provides
promising
approach
overcoming
the
limitations
conventional
vaccines.
Based
on
vaccine’s
safety
efficacy,
represents
breakthrough
among
next-generation
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(18), С. 10536 - 10536
Опубликована: Сен. 11, 2022
Over
time,
more
and
is
becoming
known
about
micro-players
of
great
significance.
This
particularly
the
case
for
microRNAs
(miRNAs;
miR),
which
have
been
found
to
participate
in
regulation
many
physiological
pathological
processes
both
humans
animals.
One
such
process
viral
infection
animals,
host
miRNAs—alone
or
conjunction
with
virus—interact
on
two
levels:
viruses
may
regulate
host’s
miRNAs
evade
its
immune
system,
while
can
play
anti-
pro-viral
roles.
The
purpose
this
comprehensive
review
present
key
involved
infections
We
summarize
data
available
literature,
indicating
that
signature
human
mainly
include
12
(i.e.,
miR-155,
miR-223,
miR-146a,
miR-122,
miR-125b,
miR-132,
miR-34a,
miR
-21,
miR-16,
miR-181
family,
let-7
miR-10a),
10
are
commonly
animals
miR-145,
miR-21,
miR-15a/miR-16
cluster,
miR-122)
context.
Knowledge
different
biological
functions
they
help
understanding
pathogenesis
diseases,
facilitating
future
development
therapeutic
agents
The
2019
pandemic,
caused
by
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
has
marked
the
spread
of
a
novel
human
coronavirus.
SARS-CoV-2
exhibited
increased
disease
severity
and
immune
evasion
across
its
variants,
molecular
mechanisms
behind
these
phenomena
remain
largely
unknown.
Conserved
elements
viral
genome,
such
as
secondary
structures
within
3′-untranslated
region
(UTR),
could
prove
crucial
in
furthering
our
understanding
host–virus
interface.
Analysis
genome
3′-UTR
revealed
potential
for
host
microRNA
(miR)
binding
sites,
allowing
sequence-specific
interactions.
In
this
study,
we
demonstrate
that
binds
cellular
miRs
miR-34a-5p,
miR-34b-5p,
miR-760-3p
vitro.
Native
gel
electrophoresis
steady-state
fluorescence
spectroscopy
were
utilized
to
biophysically
characterize
their
predicted
sites
3′-UTR.
Additionally,
investigated
2′-fluoro-d-arabinonucleic
acid
(FANA)
analogs
competitive
inhibitors
These
modulate
translation
granulin
(GRN),
interleukin-6
(IL-6),
IL-6
receptor
(IL-6R),
all
which
are
key
modulators
activators
JAK/STAT3
signaling
implicated
regulation
response.
Thus,
propose
hijacking
identify
mechanism
modulation
virus.
detailed
study
have
drive
development
antiviral
treatments
SARS-CoV-2,
through
direct
targeting
virus–host
