Burns, Год журнала: 2024, Номер unknown, С. 107194 - 107194
Опубликована: Июнь 1, 2024
Язык: Английский
Advanced Drug Delivery Reviews, Год журнала: 2024, Номер 208, С. 115237 - 115237
Опубликована: Март 5, 2024
Язык: Английский
Процитировано
27
Microbial Genomics, Год журнала: 2024, Номер 10(2)
Опубликована: Фев. 29, 2024
Molecular profiling techniques such as metagenomics, metatranscriptomics or metabolomics offer important insights into the functional diversity of microbiome. In contrast, 16S rRNA gene sequencing, a widespread and cost-effective technique to measure microbial diversity, only allows for indirect estimation function. To mitigate this, tools PICRUSt2, Tax4Fun2, PanFP MetGEM infer profiles from sequencing data using different algorithms. Prior studies have cast doubts on quality these predictions, motivating us systematically evaluate matched metagenomic datasets, simulated data. Our contribution is threefold: (i) data, we investigate if technical biases could explain discordance between inferred expected results; (ii) considering human cohorts type two diabetes, colorectal cancer obesity, test health-related differential abundance measures categories are concordant gene-inferred metagenome-derived and; (iii) since copy number an confounder in inference, customised normalisation with rrnDB database improve results. results show that gene-based inference generally do not necessary sensitivity delineate changes microbiome should thus be used care. Furthermore, outline differences individual tested recommendations tool selection.
Язык: Английский
Процитировано
24
Cureus, Год журнала: 2023, Номер unknown
Опубликована: Июль 8, 2023
The gut microbiota has been studied and continues to be a developing area in the pathognomic development of metabolic diseases like diabetes. Treatment with diet changes, addition supplements prebiotics/probiotics, impact fecal microbial transplantation can correlated targeting changes dysbiosis. Understanding impacts various anti-hyperglycemic agents such as metformin implications post-bariatric surgery on diversity emerged. These areas study are crucial understanding aspects diabetes disease progression at level inflammatory mechanisms, which may give more insight into focusing role prebiotic/probiotic potential forms prospective management that target microbiota, harbors low-grade inflammation. Intestinal dysbiosis was consistently observed mechanism change diabetic individuals, contributing reduced insulin sensitivity poor glycemic control. This systematic review carried out using Preferred Reporting Items for Systematic Reviews Meta-analyses (PRISMA) 2020 checklist. We performed literature search PubMed, Google Scholar Science Direct databases accordance eligibility criteria ultimately selected 14 articles final analysis. Scale Assessment Narrative Review Articles (SANRA) PRISMA checklist were used assess quality cross-sectional studies, traditional reviews, respectively. collected papers from 2012 2022 this review. gathered databases, study, show there is strong connection between appears exist. objective identify any dietary therapeutic alter potentially modulate sensitivity. article will discuss pathophysiological effects biodiversity therapeutics aid reversing
Язык: Английский
Процитировано
23
Experimental Hematology and Oncology, Год журнала: 2023, Номер 12(1)
Опубликована: Авг. 3, 2023
Organoids are established through in vitro 3D culture, and they can mimic the structure physiological functions of organs or tissues vivo. have attracted much attention recent years. They provide a reliable technology platform for cancer research treatment valuable preclinical model academic personalized medicine. A number studies confirmed that organoids great application prospects new drug development, screening, tumour mechanism research, precision In this review, we mainly focus on advances research. We also discussed opportunities challenges facing organoids, hoping to indicate directions development future.
Язык: Английский
Процитировано
23
Nature Reviews Endocrinology, Год журнала: 2024, Номер unknown
Опубликована: Окт. 24, 2024
Язык: Английский
Процитировано
14
Advanced Healthcare Materials, Год журнала: 2024, Номер 13(13)
Опубликована: Янв. 15, 2024
Abstract Type 2 diabetes mellitus (T2DM), obesity, and metabolic dysfunction‐associated steatotic liver disease (MASLD) are epidemiologically correlated disorders with a worldwide growing prevalence. While the mechanisms leading to onset development of these conditions not fully understood, predictive tissue representations for studying coordinated interactions between central organs that regulate energy metabolism, particularly pancreatic islets, needed. Here, dual pump‐less recirculating organ‐on‐chip platform combines human pluripotent stem cell (sc)‐derived sc‐liver sc‐islet organoids is presented. The reproduces key aspects cross‐talk both organs, including glucose levels selected hormones, supports viability functionality while preserving reduced release pro‐inflammatory cytokines. In model disruption in response treatment high lipids fructose, exhibit hallmarks steatosis insulin resistance, sc‐islets produce cytokines on‐chip. Finally, known effects anti‐diabetic drugs Taken together, provides basis functional studies T2DM, MASLD on‐chip, as well testing potential therapeutic interventions.
Язык: Английский
Процитировано
10
Biofabrication, Год журнала: 2024, Номер 16(4), С. 045029 - 045029
Опубликована: Авг. 12, 2024
Abstract Multicellular spheroids such as microtissues and organoids have demonstrated great potential for tissue engineering applications in recent years these 3D cellular units enable improved cell–cell cell–matrix interactions. Current bioprinting processes that use multicellular building blocks limited control on post printing distribution of cell or moderate throughput efficiency. In this work, we presented a laser-assisted approach able to transfer larger structures. Cartilaginous formed by human periosteum derived cells (hPDCs) were successfully bioprinted possessing high viability the capacity undergo chondrogenic differentiation printing. Smaller hPDC with diameters ranging from ∼100 150 µ m through laser-induced forward method (LIFT) however constituted challenge. For reason novel alternative was developed termed laser induced propulsion mesoscopic objects (LIPMO) whereby bioprint up 300 m. Moreover, combined process computer aided image analysis demonstrating ‘target shoot’, automated selection, multiple large single sequence. By taking advantage target shoot system, multilayered constructs containing density fabricated.
Язык: Английский
Процитировано
8
Natural Products and Bioprospecting, Год журнала: 2024, Номер 14(1)
Опубликована: Авг. 20, 2024
Arctium lappa, widely recognized as burdock, is a perennial plant that employed in the realm of traditional Chinese medicine for wide range medicinal applications. The herb rich bioactive metabolites with therapeutic potential, encompassing polyphenolic antioxidants its leaves, and flavonoids fructo-oligosaccharides underground parts. Nutraceuticals originating from botanical sources such lappa provide supplementary health advantages alongside their nutritional content have demonstrated effectiveness prevention management specific ailments. utilization root extract has exhibited encouraging outcomes addressing hepatotoxicity induced by cadmium, lead, chromium, acetaminophen, ameliorating liver damage oxidative stress. Additionally, displays properties antidiabetic, hypolipidemic, aphrodisiac, anti-rheumatic, anti-Alzheimer, various other pharmacological actions.
Язык: Английский
Процитировано
8
APL Bioengineering, Год журнала: 2024, Номер 8(1)
Опубликована: Фев. 27, 2024
Organoids significantly advanced our comprehension of organ development, function, and disease modeling. This Perspective underscores the potential heart–kidney-connected organoids in understanding intricate relationship between these vital organs, notably cardiorenal syndrome, where dysfunction one can negatively impact other. Conventional models fall short replicating this complexity, necessitating an integrated approach. By co-culturing heart kidney organoids, combined with microfluidic 3D bioprinting technologies, a more accurate representation vivo conditions be achieved. Such interconnected systems could revolutionize grasp multi-organ diseases, drive drug discovery by evaluating therapeutic agents on both organs simultaneously, reduce need for animal models. In essence, present promising avenue to delve deeper into pathophysiology underlying disorders, bridging existing knowledge gaps, advancing biomedical research.
Язык: Английский
Процитировано
7
Metabolism, Год журнала: 2024, Номер 152, С. 155786 - 155786
Опубликована: Янв. 10, 2024
Diabetes presents a pressing healthcare crisis, necessitating innovative solutions. Organoid technologies have rapidly advanced, leading to the emergence of bioengineering islet organoids as an unlimited source insulin-producing cells for treating insulin-dependent diabetes. This advancement surpasses need cadaveric transplantation. However, clinical translation this approach faces two major limitations: immature endocrine function and absence perfusable vasculature compared primary human islets. In review, we summarize latest developments in functional vitro promoting vascularization organoid grafts before after We highlight crucial roles ensuring long-term survival, maturation, functionality organoids. Additionally, discuss key considerations that must be addressed organoid-based therapy, including immaturity, undesired heterogeneity, potential tumorigenic risks.
Язык: Английский
Процитировано
6