Microbial Biotechnology,
Год журнала:
2025,
Номер
18(1)
Опубликована: Янв. 1, 2025
Using
synthetic
biology
techniques,
bacteria
have
been
engineered
to
serve
as
microrobots
for
diagnosing
diseases
and
delivering
treatments.
These
can
be
used
individually
or
in
combination
microbial
consortia.
The
components
within
these
consortia
complement
each
other,
enhancing
diagnostic
accuracy
providing
synergistic
effects
that
improve
treatment
efficacy.
application
of
therapies
cancer,
intestinal
diseases,
metabolic
disorders
underscores
their
significant
potential.
impact
on
the
host's
native
microbiota
is
crucial,
microbes
modulate
interact
with
environment,
influencing
outcomes
overall
health.
Despite
numerous
advancements,
challenges
remain.
include
ensuring
long-term
survival
safety
bacteria,
developing
new
chassis
gene
editing
techniques
non-model
strains,
minimising
potential
toxicity,
understanding
bacterial
interactions
host
microbiota.
This
mini-review
examines
current
state
disease
diagnosis
treatment,
highlighting
challenges,
future
directions
this
promising
field.
Bioactive Materials,
Год журнала:
2021,
Номер
14, С. 169 - 181
Опубликована: Дек. 17, 2021
Nanosized
extracellular
vesicles
derived
from
bacteria
contain
diverse
cargo
and
transfer
intercellular
bioactive
molecules
to
cells.
Due
their
favorable
interactions,
cell
membrane-derived
bacterial
(BEVs)
have
great
potential
become
novel
drug
delivery
platforms.
In
this
review,
we
summarize
the
biogenesis
mechanism
compositions
of
various
BEVs.
addition,
an
overview
effective
isolation
purification
techniques
BEVs
is
provided.
particular,
focus
on
application
as
nanocarriers
for
delivery.
Finally,
advances
challenges
after
providing
a
comprehensive
discussion
in
each
section.
We
believe
that
deeper
understanding
will
open
new
avenues
exploitation
applications.
Advanced Materials,
Год журнала:
2022,
Номер
34(26)
Опубликована: Март 4, 2022
Living
biological
systems,
ranging
from
single
cells
to
whole
organisms,
can
sense,
process
information,
and
actuate
in
response
changing
environmental
conditions.
Inspired
by
living
engineered
nonliving
matrices
are
brought
together,
which
gives
rise
the
technology
of
materials.
By
designing
functionalities
structures
matrices,
materials
be
created
detect
variability
surrounding
environment
adjust
their
functions
accordingly,
thereby
enabling
applications
health
monitoring,
disease
treatment,
remediation.
Hydrogels,
a
class
soft,
wet,
biocompatible
materials,
have
been
widely
used
as
for
cells,
leading
nascent
field
hydrogels.
Here,
interactions
between
hydrogel
described,
focusing
on
how
hydrogels
influence
cell
behaviors
affect
properties.
The
environments,
these
enable
versatile
applications,
also
discussed.
Finally,
current
challenges
facing
clinical
settings
highlighted.
Chemical Reviews,
Год журнала:
2022,
Номер
123(5), С. 2349 - 2419
Опубликована: Дек. 13, 2022
Recent
advances
in
synthetic
biology
and
materials
science
have
given
rise
to
a
new
form
of
materials,
namely
engineered
living
(ELMs),
which
are
composed
matter
or
cell
communities
embedded
self-regenerating
matrices
their
own
artificial
scaffolds.
Like
natural
such
as
bone,
wood,
skin,
ELMs,
possess
the
functional
capabilities
organisms,
can
grow,
self-organize,
self-repair
when
needed.
They
also
spontaneously
perform
programmed
biological
functions
upon
sensing
external
cues.
Currently,
ELMs
show
promise
for
green
energy
production,
bioremediation,
disease
treatment,
fabricating
advanced
smart
materials.
This
review
first
introduces
dynamic
features
systems
potential
developing
novel
We
then
summarize
recent
research
progress
on
emerging
design
strategies
from
both
perspectives.
Finally,
we
discuss
positive
impacts
promoting
sustainability
key
future
directions.
Infertility
is
a
rising
global
health
issue
with
far-reaching
impact
on
the
socioeconomic
livelihoods.
As
there
are
highly
complex
causes
of
male
and
female
infertility,
it
desired
to
promote
maintain
reproductive
by
integration
advanced
technologies.
Biomedical
engineering,
mature
technology
applied
in
fields
biology
care,
has
emerged
as
powerful
tool
diagnosis
treatment
infertility.
Nowadays,
various
promising
biomedical
engineering
approaches
under
investigation
address
human
can
not
only
improve
our
fundamental
understanding
sperm
follicle
development
bioengineered
devices
combined
microfabrication,
biomaterials,
relevant
cells,
but
also
be
repair
uterine,
ovary,
cervicovaginal
tissues
restore
tissue
function.
Here,
we
introduce
infertility
provide
comprehensive
summary
technologies
their
applications
medicine.
Also,
challenges
prospects
for
clinical
transformation
discussed.
We
believe
that
this
review
will
communications
between
engineers,
biologists,
clinicians
potentially
contribute
these
innovative
research
works
immediate
future.
Bioengineering,
Год журнала:
2023,
Номер
10(2), С. 204 - 204
Опубликована: Фев. 3, 2023
Over
the
last
few
years,
biopolymers
have
attracted
great
interest
in
tissue
engineering
and
regenerative
medicine
due
to
diversity
of
their
chemical,
mechanical,
physical
properties
for
fabrication
3D
scaffolds.
This
review
is
devoted
recent
advances
synthetic
natural
polymeric
scaffolds
bone
(BTE)
therapies.
The
comprehensively
discusses
implications
biological
macromolecules,
structure,
composition
used
BTE.
Various
approaches
fabricating
BTE
are
discussed,
including
solvent
casting
particle
leaching,
freeze-drying,
thermally
induced
phase
separation,
gas
foaming,
electrospinning,
sol–gel
techniques.
Rapid
prototyping
technologies
such
as
stereolithography,
fused
deposition
modeling,
selective
laser
sintering,
bioprinting
also
covered.
immunomodulatory
roles
utilized
applications
discussed.
In
addition,
features
challenges
polymer
fabricated
using
advanced
additive
manufacturing
(rapid
prototyping)
addressed
compared
conventional
subtractive
Finally,
applying
scaffold-based
treatments
practice
discussed
in-depth.
Bioactive Materials,
Год журнала:
2022,
Номер
19, С. 292 - 327
Опубликована: Апрель 23, 2022
Fabrication
of
functional
scaffolds
for
tissue
engineering
and
regenerative
medicine
applications
requires
material
systems
with
precise
control
over
cellular
performance.
3D
printing
is
a
powerful
technique
to
create
highly
complex
multicomponent
structures
well-defined
architecture
composition.
In
this
review
paper,
we
explore
extrusion-based
methods
(EBP,
i.e.,
Near
Field
Electrospinning
(NFES),
Melt
Electrowriting
(MEW),
Fused
Deposition
Modeling
(FDM),
extrusion
bioprinting)
in
terms
their
ability
produce
bio-instructive
properties.
These
provide
spatio-temporal
guidance
cells,
allowing
controlled
regeneration
maturation.
Multiple
physical
biochemical
cues
introduced
the
EBP
are
evaluated
direct
cell
alignment,
proliferation,
differentiation,
specific
ECM
production,
We
indicate
that
have
different
impacts
depending
on
system,
type
used,
or
coexistence
multiple
cues.
Therefore,
they
must
be
carefully
chosen
based
targeted
application.
propose
future
directions
materials
development,
including
such
concepts
as
metamaterials,
hybrid
living
materials,
4D
printing.
The
gathers
knowledge
essential
designing
new
response,
fabrication
advanced
engineered
tissue,
developing
better
understanding
biology,
especially
response
biomaterial.
Advanced Functional Materials,
Год журнала:
2022,
Номер
32(26)
Опубликована: Март 22, 2022
Abstract
The
use
of
cell‐mediated
chemistry
is
an
emerging
strategy
that
exploits
the
metabolic
processes
living
cells
to
develop
biomimetic
materials
with
advanced
functionalities
and
enhanced
biocompatibility.
Here,
a
concept
catalytic
process
for
forming
protective
nano‐shells
on
individual
probiotic
demonstrated.
This
leveraged
by
cell
environment
induce
oxidative
polymerization
phenolic
compounds,
simultaneously
these
polymers
assemble
form
nano‐coatings
around
surfaces.
detailed
analysis
reveals
oxidation
triggered
essential
nutrient
(manganese)
cells,
which
significantly
increases
rate
compounds.
coatings,
encapsulating
each
in
nanometre
scale,
demonstrate
excellent
biocompatibility
biodegradability.
Additionally,
situ
encapsulated
display
improved
gastric
tolerance
up
≈1.4
times
higher
than
native
adhesion
as
high
≈1.6
onto
model
intestinal
epithelial
cells.
Finally,
coated
exhibit
antioxidant
activity
feature.
Overall,
this
method
provides
unique
approach
improve
delivery
using
machinery
engineer
nanocoatings
benefits
new
functionalities.
