Chemical Science,
Год журнала:
2023,
Номер
14(11), С. 2928 - 2934
Опубликована: Янв. 1, 2023
Small-molecule
probes
emitting
in
the
second
near-infrared
window
(NIR-II)
are
attracting
great
attention
because
of
their
deep-tissue
imaging
ability.
However,
developing
NIR-II
fluorogenic
(off-on)
with
good
water
solubility
remains
a
challenge
due
to
lack
facile
approach.
Herein
we
first
report
combination
changeable
π-conjugation
and
hydrophilic
groups
as
an
effective
strategy
for
water-soluble
probes.
With
strategy,
new
fluorophores
prepared,
among
which
NIR-II-F2
NIR-II-F3
show
superior
stability
bright
fluorescence
aqueous
media,
thus
used
design
two
leucine
aminopeptidase
(LAP).
The
excellent
performance
real
bio-environments
is
demonstrated
by
mouse
vasculatures
organs
NIR-II-F2,
LAP
drug-induced
liver
injury
mice
one
enzymatic
probes;
however,
water-insoluble
dyes
cannot
achieve
such
vivo
under
same
conditions.
Our
may
be
helpful
further
organic
other
analytes.
Advanced Materials,
Год журнала:
2023,
Номер
35(20)
Опубликована: Март 3, 2023
Molecular
fluorophores
with
the
second
near-infrared
(NIR-II)
emission
hold
great
potential
for
deep-tissue
bioimaging
owing
to
their
excellent
biocompatibility
and
high
resolution.
Recently,
J-aggregates
are
used
construct
long-wavelength
NIR-II
emitters
as
optical
bands
show
remarkable
red
shifts
upon
forming
water-dispersible
nano-aggregates.
However,
wide
applications
in
fluorescence
imaging
impeded
by
limited
varieties
of
J-type
backbone
serious
quenching.
Herein,
a
bright
benzo[c]thiophene
(BT)
J-aggregate
fluorophore
(BT6)
anti-quenching
effect
is
reported
highly
efficient
phototheranostics.
The
BT
manipulated
have
Stokes
shift
over
400
nm
aggregation-induced
(AIE)
property
conquering
self-quenching
issue
fluorophores.
Upon
BT6
assemblies
an
aqueous
environment,
absorption
800
1000
boosted
more
than
41
26
folds,
respectively.
In
vivo
visualization
whole-body
blood
vessel
imaging-guided
phototherapy
results
verify
that
NPs
agent
cancer
This
work
develops
strategy
precisely
properties
biomedical
applications.
Proceedings of the National Academy of Sciences,
Год журнала:
2022,
Номер
119(52)
Опубликована: Дек. 19, 2022
Capturing
mitochondria's
intricate
and
dynamic
structure
poses
a
daunting
challenge
for
optical
nanoscopy.
Different
labeling
strategies
have
been
demonstrated
live-cell
stimulated
emission
depletion
(STED)
microscopy
of
mitochondria,
but
orthogonal
are
yet
to
be
established,
image
acquisition
has
suffered
either
from
photodamage
the
organelles
or
rapid
photobleaching.
Therefore,
nanoscopy
mitochondria
largely
restricted
two-dimensional
(2D)
single-color
recordings
cancer
cells.
Here,
by
conjugation
cyclooctatetraene
(COT)
benzo-fused
cyanine
dye,
we
report
mitochondrial
inner
membrane
(IM)
fluorescent
marker,
PK
Mito
Orange
(PKMO),
featuring
efficient
STED
at
775
nm,
strong
photostability,
markedly
reduced
phototoxicity.
PKMO
enables
super-resolution
(SR)
IM
dynamics
extended
periods
in
immortalized
mammalian
cell
lines,
primary
cells,
organoids.
Photostability
phototoxicity
open
door
three-dimensional
(3D)
3D
analysis
convoluted
IM.
is
optically
with
green
far-red
markers,
allowing
multiplexed
using
commercial
microscopes.
Using
multi-color
microscopy,
demonstrate
that
imaging
can
capture
interactions
different
cellular
components
such
as
endoplasmic
reticulum
(ER)
cytoskeleton,
Bcl-2-associated
X
protein
(BAX)-induced
apoptotic
process,
crista
phenotypes
genetically
modified
all
sub-100
nm
resolution.
Thereby,
this
work
offers
versatile
tool
studying
architecture
manner.
Chemical Society Reviews,
Год журнала:
2022,
Номер
51(18), С. 7692 - 7714
Опубликована: Янв. 1, 2022
This
paper
reviews
organic
and
inorganic
species
as
building
blocks
various
design
strategies
of
NIR-II
nanohybrids
for
intensity-based
lifetime-based
bioimaging
biosensing.
Chemical Science,
Год журнала:
2022,
Номер
13(38), С. 11280 - 11293
Опубликована: Янв. 1, 2022
Polymethine
cyanine
dyes,
as
the
most
important
class
of
organic
near-infrared-II
(NIR-II)
fluorophores,
recently
received
increasing
attention
due
to
their
high
molar
extinction
coefficients,
intensive
fluorescence
brightness,
and
flexible
wavelength
tunability
for
fluorescent
bioimaging
applications.
Very
recently,
remarkable
advances
have
been
made
in
development
NIR-II
polymethine
fluorophores
with
improved
optical
performance,
mainly
including
tunable
fluorescence,
water
solubility
stability.
In
this
review,
we
summarize
recent
research
molecular
tailoring
design
strategies
then
emphasize
representative
biosensing
The
potential
challenges
perspectives
emerging
field
are
also
discussed.
This
review
may
provide
guidance
reference
further
high-performance
boost
clinical
translation
future.
Journal of the American Chemical Society,
Год журнала:
2022,
Номер
144(46), С. 21010 - 21015
Опубликована: Окт. 25, 2022
Visualizing
biomolecules
such
as
enzymes
in
the
deep
tissue
of
living
organisms
via
molecular
ratiometric
fluorescent
probes
second
near-infrared
window
(NIR-II)
with
a
built-in
self-calibration
function
can
provide
reliable
information
about
relevant
pathophysiological
processes
directly
but
so
far
is
not
feasible
due
to
lack
fluorescence
modulation
strategy
NIR-II
window.
Here
we
present
platform
Py-2
by
integrating
rhodamine
6G
scaffold
and
polymethine.
The
maximal
emission
wavelength
was
1010
nm
blue-shifted
945
when
its
secondary
amine
acylated.
Based
on
Py-2,
two
probes,
nitroreductase-responsive
Rap-N
ROS-responsive
Rap-R,
were
constructed
successfully
demonstrated
vitro
vivo.
Overall,
this
report
presents
unique
approach
developing
for
vivo
biosensing.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(22), С. 12013 - 12022
Опубликована: Май 22, 2023
The
shortwave
infrared
(SWIR)
spectral
region
beyond
1200
nm
offers
optimal
tissue
penetration
depth
and
has
broad
potential
in
diagnosis,
therapy,
surgery.
Here,
we
devised
a
novel
class
of
fluorochromic
scaffold,
i.e.,
tetra-benzannulated
xanthenoid
(EC7).
EC7
absorbs/emits
maximally
at
1204/1290
CH2Cl2
exhibits
an
unparalleled
molar
absorptivity
3.91
×
105
cm–1
M–1
high
transparency
to
light
400–900
nm.
It
also
exhibited
resistance
toward
both
photobleaching
symmetry
breaking
due
its
unique
structural
rigidity.
is
feasible
for
vivo
bioimaging
particularly
suitable
couple
with
the
shorter-wavelength
analogues
high-contrast
multiplexing.
High-contrast
dual-channel
intraoperative
imaging
hepatobiliary
system
three-channel
intestine,
stomach,
vasculature
were
showcased.
benchmark
fluorochrome
facile
biomedical
exploitation
SWIR
