Orchestrated Strategies for Developing Fluorophores for NIR‐II Imaging DOI

Xin Feng,

Wei Long, Yanlin Liu

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(24)

Published: May 11, 2023

Abstract Fluorescence imaging (FLI), a non‐invasive, real‐time, and highly sensitive modality, allows for investigating the molecular/cellular level activities to understand physiological functions diseases. The emergence of second near‐infrared window (NIR‐II, 1000–1700 nm) has endowed fluorescence with deeper tissue penetration unprecedented clarity. Among various NIR‐II fluorophores, organic fluorescent probes have occupied pivotal position in bioimaging due their higher biocompatibility, safety, potential clinical applications compared those inorganic probes. To obtain high‐quality dyes, diverse strategies been taken. In this review, different optimizing fluorophores are summarized, including traditional chemical modifications, emerging bioengineering operations, which not previously elaborated on summarized. Moreover, highlighted using endogenous serum proteins even exogenous gene‐editing proteins, would provide fresh insights design good‐performance dyes help develop translation future. A critical perspective direction disease is also proposed.

Language: Английский

Near-infrared luminescence high-contrast in vivo biomedical imaging DOI Open Access
Ying Chen, Shangfeng Wang, Fan Zhang

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(1), P. 60 - 78

Published: Jan. 19, 2023

Language: Английский

Citations

277
Fluorescence-amplified nanocrystals in the second near-infrared window for in vivo real-time dynamic multiplexed imaging DOI
Yiwei Yang, Ying Chen, Peng Pei

et al.

Nature Nanotechnology, Journal Year: 2023, Volume and Issue: 18(10), P. 1195 - 1204

Published: June 22, 2023

Language: Английский

Citations

131
Fluorescence image-guided tumour surgery DOI Open Access
Kun Wang, Yang Du, Zeyu Zhang

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(3), P. 161 - 179

Published: Feb. 3, 2023

Language: Английский

Citations

109
Anti‐Quenching NIR‐II J‐Aggregates of Benzo[c]thiophene Fluorophore for Highly Efficient Bioimaging and Phototheranostics DOI
Ka‐Wai Lee,

Yijian Gao,

Wei‐Chih Wei

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(20)

Published: March 3, 2023

Molecular fluorophores with the second near-infrared (NIR-II) emission hold great potential for deep-tissue bioimaging owing to their excellent biocompatibility and high resolution. Recently, J-aggregates are used construct long-wavelength NIR-II emitters as optical bands show remarkable red shifts upon forming water-dispersible nano-aggregates. However, wide applications in fluorescence imaging impeded by limited varieties of J-type backbone serious quenching. Herein, a bright benzo[c]thiophene (BT) J-aggregate fluorophore (BT6) anti-quenching effect is reported highly efficient phototheranostics. The BT manipulated have Stokes shift over 400 nm aggregation-induced (AIE) property conquering self-quenching issue fluorophores. Upon BT6 assemblies an aqueous environment, absorption 800 1000 boosted more than 41 26 folds, respectively. In vivo visualization whole-body blood vessel imaging-guided phototherapy results verify that NPs agent cancer This work develops strategy precisely properties biomedical applications.

Language: Английский

Citations

108
Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain DOI Creative Commons
Tianyan Liu, Till Stephan, Peng Chen

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(52)

Published: Dec. 19, 2022

Capturing mitochondria's intricate and dynamic structure poses a daunting challenge for optical nanoscopy. Different labeling strategies have been demonstrated live-cell stimulated emission depletion (STED) microscopy of mitochondria, but orthogonal are yet to be established, image acquisition has suffered either from photodamage the organelles or rapid photobleaching. Therefore, nanoscopy mitochondria largely restricted two-dimensional (2D) single-color recordings cancer cells. Here, by conjugation cyclooctatetraene (COT) benzo-fused cyanine dye, we report mitochondrial inner membrane (IM) fluorescent marker, PK Mito Orange (PKMO), featuring efficient STED at 775 nm, strong photostability, markedly reduced phototoxicity. PKMO enables super-resolution (SR) IM dynamics extended periods in immortalized mammalian cell lines, primary cells, organoids. Photostability phototoxicity open door three-dimensional (3D) 3D analysis convoluted IM. is optically with green far-red markers, allowing multiplexed using commercial microscopes. Using multi-color microscopy, demonstrate that imaging can capture interactions different cellular components such as endoplasmic reticulum (ER) cytoskeleton, Bcl-2-associated X protein (BAX)-induced apoptotic process, crista phenotypes genetically modified all sub-100 nm resolution. Thereby, this work offers versatile tool studying architecture manner.

Language: Английский

Citations

92
Management of fluorescent organic/inorganic nanohybrids for biomedical applications in the NIR-II region DOI Creative Commons

Benhao Li,

Mengyao Zhao, Jing Lin

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(18), P. 7692 - 7714

Published: Jan. 1, 2022

This paper reviews organic and inorganic species as building blocks various design strategies of NIR-II nanohybrids for intensity-based lifetime-based bioimaging biosensing.

Language: Английский

Citations

85
The pursuit of polymethine fluorophores with NIR-II emission and high brightness forin vivoapplications DOI Creative Commons
Xuan Zhao, Fan Zhang, Zuhai Lei

et al.

Chemical Science, Journal Year: 2022, Volume and Issue: 13(38), P. 11280 - 11293

Published: Jan. 1, 2022

Polymethine cyanine dyes, as the most important class of organic near-infrared-II (NIR-II) fluorophores, recently received increasing attention due to their high molar extinction coefficients, intensive fluorescence brightness, and flexible wavelength tunability for fluorescent bioimaging applications. Very recently, remarkable advances have been made in development NIR-II polymethine fluorophores with improved optical performance, mainly including tunable fluorescence, water solubility stability. In this review, we summarize recent research molecular tailoring design strategies then emphasize representative biosensing The potential challenges perspectives emerging field are also discussed. This review may provide guidance reference further high-performance boost clinical translation future.

Language: Английский

Citations

78
Polymethine Molecular Platform for Ratiometric Fluorescent Probes in the Second near-Infrared Window DOI
Qingchun Lan, Peng Yu,

Kui Yan

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(46), P. 21010 - 21015

Published: Oct. 25, 2022

Visualizing biomolecules such as enzymes in the deep tissue of living organisms via molecular ratiometric fluorescent probes second near-infrared window (NIR-II) with a built-in self-calibration function can provide reliable information about relevant pathophysiological processes directly but so far is not feasible due to lack fluorescence modulation strategy NIR-II window. Here we present platform Py-2 by integrating rhodamine 6G scaffold and polymethine. The maximal emission wavelength was 1010 nm blue-shifted 945 when its secondary amine acylated. Based on Py-2, two probes, nitroreductase-responsive Rap-N ROS-responsive Rap-R, were constructed successfully demonstrated vitro vivo. Overall, this report presents unique approach developing for vivo biosensing.

Language: Английский

Citations

75
Near-infrared II fluorescence imaging DOI
E. Schmidt, Zihao Ou, Erving Ximendes

et al.

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: April 4, 2024

Language: Английский

Citations

73
Rigid and Photostable Shortwave Infrared Dye Absorbing/Emitting beyond 1200 nm for High-Contrast Multiplexed Imaging DOI

Ruwei Wei,

Yan Dong, Xueli Wang

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(22), P. 12013 - 12022

Published: May 22, 2023

The shortwave infrared (SWIR) spectral region beyond 1200 nm offers optimal tissue penetration depth and has broad potential in diagnosis, therapy, surgery. Here, we devised a novel class of fluorochromic scaffold, i.e., tetra-benzannulated xanthenoid (EC7). EC7 absorbs/emits maximally at 1204/1290 CH2Cl2 exhibits an unparalleled molar absorptivity 3.91 × 105 cm–1 M–1 high transparency to light 400–900 nm. It also exhibited resistance toward both photobleaching symmetry breaking due its unique structural rigidity. is feasible for vivo bioimaging particularly suitable couple with the shorter-wavelength analogues high-contrast multiplexing. High-contrast dual-channel intraoperative imaging hepatobiliary system three-channel intestine, stomach, vasculature were showcased. benchmark fluorochrome facile biomedical exploitation SWIR

Language: Английский

Citations

58