High-fidelity imaging of a tumour-associated lysosomal enzyme with an acceptor engineering-boosted near-infrared fluorescent probe DOI Creative Commons
Feng Bin,

Feiyi Chu,

Yanpeng Fang

и другие.

Chemical Science, Год журнала: 2024, Номер 15(19), С. 7324 - 7331

Опубликована: Янв. 1, 2024

This work reports an acceptor engineering-boosted near-infrared fluorescent probe for high-fidelity imaging of tumour-associated β-galactosidase specifically in lysosomes and vivo .

Язык: Английский

Activity-based NIR fluorescent probes based on the versatile hemicyanine scaffold: design strategy, biomedical applications, and outlook DOI
Haidong Li, Heejeong Kim, Feng Xu

и другие.

Chemical Society Reviews, Год журнала: 2022, Номер 51(5), С. 1795 - 1835

Опубликована: Янв. 1, 2022

The discovery of a near-infrared (NIR, 650-900 nm) fluorescent chromophore hemicyanine dye with high structural tailorability is great significance in the field detection, bioimaging, and medical therapeutic applications. It exhibits many outstanding advantages including absorption emission NIR region, tunable spectral properties, photostability as well large Stokes shift. These properties are superior to those conventional fluorogens, such coumarin, fluorescein, naphthalimides, rhodamine, cyanine. Researchers have made remarkable progress developing activity-based multifunctional probes based on skeletons for monitoring vital biomolecules living systems through output fluorescence/photoacoustic signals, integration diagnosis treatment diseases using chemotherapy or photothermal/photodynamic therapy combination therapy. achievements prompted researchers develop more smart fluorogen template. In this review, we begin by describing brief history dyes, synthetic approaches, design strategies functional probes. Then, selected hemicyanine-based that can detect ions, small biomolecules, overexpressed enzymes diagnostic reagents systematically highlighted. Finally, potential drawbacks outlook future investigation clinical medicine transformation activatable also discussed.

Язык: Английский

Процитировано

356

Mitochondria-targeted smart AIEgens: Imaging and therapeutics DOI
Haidong Li, Heejeong Kim, Cheng Zhang

и другие.

Coordination Chemistry Reviews, Год журнала: 2022, Номер 473, С. 214818 - 214818

Опубликована: Сен. 21, 2022

Язык: Английский

Процитировано

95

Biomarker-activatable probes based on smart AIEgens for fluorescence and optoacoustic imaging DOI
Juan Ouyang, Lihe Sun, Fang Zeng

и другие.

Coordination Chemistry Reviews, Год журнала: 2022, Номер 458, С. 214438 - 214438

Опубликована: Фев. 3, 2022

Язык: Английский

Процитировано

90

Noncancerous disease-targeting AIEgens DOI Creative Commons
Yanhong Duo, Guanghong Luo, Wentao Zhang

и другие.

Chemical Society Reviews, Год журнала: 2023, Номер 52(3), С. 1024 - 1067

Опубликована: Янв. 1, 2023

Schematic diagram of noncancerous disease-targeting AIEgens.

Язык: Английский

Процитировано

66

Activatable Probes for Ratiometric Imaging of Endogenous Biomarkers In Vivo DOI
Qinrui Fu, Xiao Yang, Mengzhen Wang

и другие.

ACS Nano, Год журнала: 2024, Номер 18(5), С. 3916 - 3968

Опубликована: Янв. 23, 2024

Dynamic variations in the concentration and abnormal distribution of endogenous biomarkers are strongly associated with multiple physiological pathological states. Therefore, it is crucial to design imaging systems capable real-time detection dynamic changes for accurate diagnosis effective treatment diseases. Recently, ratiometric has emerged as a widely used technique sensing due its advantage circumventing limitations inherent conventional intensity-dependent signal readout methods while also providing built-in self-calibration correction. Here, recent progress probes their applications outlined. Ratiometric classified according mechanisms, photoacoustic imaging, optical including photoluminescence self-luminescence magnetic resonance dual-modal discussed. The such pH, reactive oxygen species (ROS), nitrogen (RNS), glutathione (GSH), gas molecules, enzymes, metal ions, hypoxia discussed detail. Additionally, this Review presents an overview challenges faced field along future research directions.

Язык: Английский

Процитировано

34

Enzyme‐Mediated In Situ Self‐Assembly AIE Probes: Mapping the Distribution of Acetylcholinesterase in Aging Mice Brain DOI Open Access
Zhirong Zhu, Shanshan Hu, Youheng Zhang

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

Abstract Acetylcholinesterase (AChE) exhibits profound association with the underlying pathological mechanisms of aging‐related disorders. However, revealing spatial distribution AChE among whole‐brain remains an uncharted territory, posing a substantial challenge. Herein, dual regulation strategy to obtain aggregation‐induced emission (AIE)‐activable probe for high‐fidility mapping in brain is proposed. The well‐tailored (AChE‐QM‐2) consists four components: AChE‐specific cleavage unit (dimethylcarbamate), self‐immolative linker ( p ‐hydroxybenzyl alcohol), AIE framework (Py‐QM), and electron‐donor group (EDG). hydrophilic pyridine salt AChE‐QM‐2 improves its dispersity, collaborating EDG lower Δ E S1‐T1 HOMO‐LUMO energy gap, resulting reduced fluorescence quantum yield Φ f ) initial off‐fluorescence state. Moreover, optimizing EDGs further enhance probe's responsive performance extend wavelength. Upon AChE‐activization, undergoes deionization, molecule aggregation increased , triggering amplified signal. has successfully realized monitoring endogenous PC12 cells, tissues living mice. Furthermore, utilizing light‐sheet microscopy, intracranial AChE, providing highly specialized visualization localization within brain, achieved first time. This study satisfactorily demonstrates valuable designing ultra‐sensitive enzymatic probes, highlighting their potential precise analysis fundamental life science research.

Язык: Английский

Процитировано

4

Recent advances in small-molecule fluorescent probes for diagnosis of cancer cells/tissues DOI
Kun Wang, Caiyun Liu,

Hanchuang Zhu

и другие.

Coordination Chemistry Reviews, Год журнала: 2022, Номер 477, С. 214946 - 214946

Опубликована: Дек. 9, 2022

Язык: Английский

Процитировано

71

Specific Activation of Photosensitizer with Extrinsic Enzyme for Precisive Photodynamic Therapy DOI
Junlong Xiong, Jacky C. H. Chu, Wing‐Ping Fong

и другие.

Journal of the American Chemical Society, Год журнала: 2022, Номер 144(23), С. 10647 - 10658

Опубликована: Май 31, 2022

Delivery of functional proteins into the intracellular space has been a challenging task that could lead to myriad therapeutic applications. We report herein novel bioconjugation strategy for enzyme modification and selective delivery cancer cells lock-and-key-type activation photosensitizers. Using bifunctional linker containing bis(bromomethyl)phenyl group an o-phthalaldehyde moiety, it induce cyclization peptide sequence Ac-NH-CRGDfC-CONH2 through site-specific dibenzylation with two cysteine residues further coupling β-galactosidase via phthalaldehyde-amine capture reaction. This facile two-step one-pot procedure enabled preparation cyclic RGD-modified readily, which be internalized selectively αvβ3 integrin-overexpressed cells. Upon encountering intrinsically quenched distyryl boron dipyrromethene-based photosensitizer conjugated galactose moiety self-immolative inside cells, extrinsic induced specific cleavage β-galactosidic bond followed by self-immolation release activated derivative, thereby restoring photodynamic activities causing cell death effectively. The high specificity this enzyme-activated photosensitizing system was also demonstrated in vivo using nude mice bearing integrin-positive U87-MG tumor. at tumor site resulted lighting up complete eradication upon laser irradiation, while native β-galactosidase, effects were largely reduced. In contrast conventional intrinsic enzymes, activatable approach can minimize nonspecific toward precisive therapy.

Язык: Английский

Процитировано

55

Optimized strategies of ROS-based nanodynamic therapies for tumor theranostics DOI

Yifan Di,

Ruizhu Deng,

Zhu Liu

и другие.

Biomaterials, Год журнала: 2023, Номер 303, С. 122391 - 122391

Опубликована: Ноя. 15, 2023

Язык: Английский

Процитировано

42

Fluorogenic Reactions in Chemical Biology: Seeing Chemistry in Cells DOI Creative Commons
Yanyan Chen, Hao Jiang, Tingting Hao

и другие.

Chemical & Biomedical Imaging, Год журнала: 2023, Номер 1(7), С. 590 - 619

Опубликована: Май 30, 2023

Among the recent development of click chemistry and bioorthogonal chemistry, fluorogenic reactions occupy a unique place in that fluorescence is generated from nonfluorescent reactants, thereby rendering them highly useful convenient no-wash live-cell imaging. This topic was extensively reviewed 2010 by Wang et al. ( Chem. Soc. Rev. 2010, 39, 1233−1239) 2014 Lin Curr. Opin. Biol. 2014, 21, 89−95). review presents comprehensive up-to-date overview on past decade. The are classified into four major categories basis mechanisms generation. Representative examples each type discussed briefly terms structure, mechanism, advantages. We describe latest applications chemical biology. In end, future opportunities challenges this field tentatively proposed.

Язык: Английский

Процитировано

39