Chemical Science, Год журнала: 2024, Номер 15(19), С. 7324 - 7331
Опубликована: Янв. 1, 2024
This work reports an acceptor engineering-boosted near-infrared fluorescent probe for high-fidelity imaging of tumour-associated β-galactosidase specifically in lysosomes and vivo .
Язык: Английский
Chemical Society Reviews, Год журнала: 2022, Номер 51(5), С. 1795 - 1835
Опубликована: Янв. 1, 2022
The discovery of a near-infrared (NIR, 650-900 nm) fluorescent chromophore hemicyanine dye with high structural tailorability is great significance in the field detection, bioimaging, and medical therapeutic applications. It exhibits many outstanding advantages including absorption emission NIR region, tunable spectral properties, photostability as well large Stokes shift. These properties are superior to those conventional fluorogens, such coumarin, fluorescein, naphthalimides, rhodamine, cyanine. Researchers have made remarkable progress developing activity-based multifunctional probes based on skeletons for monitoring vital biomolecules living systems through output fluorescence/photoacoustic signals, integration diagnosis treatment diseases using chemotherapy or photothermal/photodynamic therapy combination therapy. achievements prompted researchers develop more smart fluorogen template. In this review, we begin by describing brief history dyes, synthetic approaches, design strategies functional probes. Then, selected hemicyanine-based that can detect ions, small biomolecules, overexpressed enzymes diagnostic reagents systematically highlighted. Finally, potential drawbacks outlook future investigation clinical medicine transformation activatable also discussed.
Язык: Английский
Процитировано
356
Coordination Chemistry Reviews, Год журнала: 2022, Номер 473, С. 214818 - 214818
Опубликована: Сен. 21, 2022
Язык: Английский
Процитировано
95
Coordination Chemistry Reviews, Год журнала: 2022, Номер 458, С. 214438 - 214438
Опубликована: Фев. 3, 2022
Язык: Английский
Процитировано
90
Chemical Society Reviews, Год журнала: 2023, Номер 52(3), С. 1024 - 1067
Опубликована: Янв. 1, 2023
Schematic diagram of noncancerous disease-targeting AIEgens.
Язык: Английский
Процитировано
66
ACS Nano, Год журнала: 2024, Номер 18(5), С. 3916 - 3968
Опубликована: Янв. 23, 2024
Dynamic variations in the concentration and abnormal distribution of endogenous biomarkers are strongly associated with multiple physiological pathological states. Therefore, it is crucial to design imaging systems capable real-time detection dynamic changes for accurate diagnosis effective treatment diseases. Recently, ratiometric has emerged as a widely used technique sensing due its advantage circumventing limitations inherent conventional intensity-dependent signal readout methods while also providing built-in self-calibration correction. Here, recent progress probes their applications outlined. Ratiometric classified according mechanisms, photoacoustic imaging, optical including photoluminescence self-luminescence magnetic resonance dual-modal discussed. The such pH, reactive oxygen species (ROS), nitrogen (RNS), glutathione (GSH), gas molecules, enzymes, metal ions, hypoxia discussed detail. Additionally, this Review presents an overview challenges faced field along future research directions.
Язык: Английский
Процитировано
34
Advanced Functional Materials, Год журнала: 2025, Номер unknown
Опубликована: Янв. 10, 2025
Abstract Acetylcholinesterase (AChE) exhibits profound association with the underlying pathological mechanisms of aging‐related disorders. However, revealing spatial distribution AChE among whole‐brain remains an uncharted territory, posing a substantial challenge. Herein, dual regulation strategy to obtain aggregation‐induced emission (AIE)‐activable probe for high‐fidility mapping in brain is proposed. The well‐tailored (AChE‐QM‐2) consists four components: AChE‐specific cleavage unit (dimethylcarbamate), self‐immolative linker ( p ‐hydroxybenzyl alcohol), AIE framework (Py‐QM), and electron‐donor group (EDG). hydrophilic pyridine salt AChE‐QM‐2 improves its dispersity, collaborating EDG lower Δ E S1‐T1 HOMO‐LUMO energy gap, resulting reduced fluorescence quantum yield Φ f ) initial off‐fluorescence state. Moreover, optimizing EDGs further enhance probe's responsive performance extend wavelength. Upon AChE‐activization, undergoes deionization, molecule aggregation increased , triggering amplified signal. has successfully realized monitoring endogenous PC12 cells, tissues living mice. Furthermore, utilizing light‐sheet microscopy, intracranial AChE, providing highly specialized visualization localization within brain, achieved first time. This study satisfactorily demonstrates valuable designing ultra‐sensitive enzymatic probes, highlighting their potential precise analysis fundamental life science research.
Язык: Английский
Процитировано
4
Coordination Chemistry Reviews, Год журнала: 2022, Номер 477, С. 214946 - 214946
Опубликована: Дек. 9, 2022
Язык: Английский
Процитировано
71
Journal of the American Chemical Society, Год журнала: 2022, Номер 144(23), С. 10647 - 10658
Опубликована: Май 31, 2022
Delivery of functional proteins into the intracellular space has been a challenging task that could lead to myriad therapeutic applications. We report herein novel bioconjugation strategy for enzyme modification and selective delivery cancer cells lock-and-key-type activation photosensitizers. Using bifunctional linker containing bis(bromomethyl)phenyl group an o-phthalaldehyde moiety, it induce cyclization peptide sequence Ac-NH-CRGDfC-CONH2 through site-specific dibenzylation with two cysteine residues further coupling β-galactosidase via phthalaldehyde-amine capture reaction. This facile two-step one-pot procedure enabled preparation cyclic RGD-modified readily, which be internalized selectively αvβ3 integrin-overexpressed cells. Upon encountering intrinsically quenched distyryl boron dipyrromethene-based photosensitizer conjugated galactose moiety self-immolative inside cells, extrinsic induced specific cleavage β-galactosidic bond followed by self-immolation release activated derivative, thereby restoring photodynamic activities causing cell death effectively. The high specificity this enzyme-activated photosensitizing system was also demonstrated in vivo using nude mice bearing integrin-positive U87-MG tumor. at tumor site resulted lighting up complete eradication upon laser irradiation, while native β-galactosidase, effects were largely reduced. In contrast conventional intrinsic enzymes, activatable approach can minimize nonspecific toward precisive therapy.
Язык: Английский
Процитировано
55
Biomaterials, Год журнала: 2023, Номер 303, С. 122391 - 122391
Опубликована: Ноя. 15, 2023
Язык: Английский
Процитировано
42
Chemical & Biomedical Imaging, Год журнала: 2023, Номер 1(7), С. 590 - 619
Опубликована: Май 30, 2023
Among the recent development of click chemistry and bioorthogonal chemistry, fluorogenic reactions occupy a unique place in that fluorescence is generated from nonfluorescent reactants, thereby rendering them highly useful convenient no-wash live-cell imaging. This topic was extensively reviewed 2010 by Wang et al. ( Chem. Soc. Rev. 2010, 39, 1233−1239) 2014 Lin Curr. Opin. Biol. 2014, 21, 89−95). review presents comprehensive up-to-date overview on past decade. The are classified into four major categories basis mechanisms generation. Representative examples each type discussed briefly terms structure, mechanism, advantages. We describe latest applications chemical biology. In end, future opportunities challenges this field tentatively proposed.
Язык: Английский
Процитировано
39