Chemical Science,
Год журнала:
2024,
Номер
15(19), С. 7324 - 7331
Опубликована: Янв. 1, 2024
This
work
reports
an
acceptor
engineering-boosted
near-infrared
fluorescent
probe
for
high-fidelity
imaging
of
tumour-associated
β-galactosidase
specifically
in
lysosomes
and
vivo
.
Chemical Society Reviews,
Год журнала:
2022,
Номер
51(5), С. 1795 - 1835
Опубликована: Янв. 1, 2022
The
discovery
of
a
near-infrared
(NIR,
650-900
nm)
fluorescent
chromophore
hemicyanine
dye
with
high
structural
tailorability
is
great
significance
in
the
field
detection,
bioimaging,
and
medical
therapeutic
applications.
It
exhibits
many
outstanding
advantages
including
absorption
emission
NIR
region,
tunable
spectral
properties,
photostability
as
well
large
Stokes
shift.
These
properties
are
superior
to
those
conventional
fluorogens,
such
coumarin,
fluorescein,
naphthalimides,
rhodamine,
cyanine.
Researchers
have
made
remarkable
progress
developing
activity-based
multifunctional
probes
based
on
skeletons
for
monitoring
vital
biomolecules
living
systems
through
output
fluorescence/photoacoustic
signals,
integration
diagnosis
treatment
diseases
using
chemotherapy
or
photothermal/photodynamic
therapy
combination
therapy.
achievements
prompted
researchers
develop
more
smart
fluorogen
template.
In
this
review,
we
begin
by
describing
brief
history
dyes,
synthetic
approaches,
design
strategies
functional
probes.
Then,
selected
hemicyanine-based
that
can
detect
ions,
small
biomolecules,
overexpressed
enzymes
diagnostic
reagents
systematically
highlighted.
Finally,
potential
drawbacks
outlook
future
investigation
clinical
medicine
transformation
activatable
also
discussed.
ACS Nano,
Год журнала:
2024,
Номер
18(5), С. 3916 - 3968
Опубликована: Янв. 23, 2024
Dynamic
variations
in
the
concentration
and
abnormal
distribution
of
endogenous
biomarkers
are
strongly
associated
with
multiple
physiological
pathological
states.
Therefore,
it
is
crucial
to
design
imaging
systems
capable
real-time
detection
dynamic
changes
for
accurate
diagnosis
effective
treatment
diseases.
Recently,
ratiometric
has
emerged
as
a
widely
used
technique
sensing
due
its
advantage
circumventing
limitations
inherent
conventional
intensity-dependent
signal
readout
methods
while
also
providing
built-in
self-calibration
correction.
Here,
recent
progress
probes
their
applications
outlined.
Ratiometric
classified
according
mechanisms,
photoacoustic
imaging,
optical
including
photoluminescence
self-luminescence
magnetic
resonance
dual-modal
discussed.
The
such
pH,
reactive
oxygen
species
(ROS),
nitrogen
(RNS),
glutathione
(GSH),
gas
molecules,
enzymes,
metal
ions,
hypoxia
discussed
detail.
Additionally,
this
Review
presents
an
overview
challenges
faced
field
along
future
research
directions.
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 10, 2025
Abstract
Acetylcholinesterase
(AChE)
exhibits
profound
association
with
the
underlying
pathological
mechanisms
of
aging‐related
disorders.
However,
revealing
spatial
distribution
AChE
among
whole‐brain
remains
an
uncharted
territory,
posing
a
substantial
challenge.
Herein,
dual
regulation
strategy
to
obtain
aggregation‐induced
emission
(AIE)‐activable
probe
for
high‐fidility
mapping
in
brain
is
proposed.
The
well‐tailored
(AChE‐QM‐2)
consists
four
components:
AChE‐specific
cleavage
unit
(dimethylcarbamate),
self‐immolative
linker
(
p
‐hydroxybenzyl
alcohol),
AIE
framework
(Py‐QM),
and
electron‐donor
group
(EDG).
hydrophilic
pyridine
salt
AChE‐QM‐2
improves
its
dispersity,
collaborating
EDG
lower
Δ
E
S1‐T1
HOMO‐LUMO
energy
gap,
resulting
reduced
fluorescence
quantum
yield
Φ
f
)
initial
off‐fluorescence
state.
Moreover,
optimizing
EDGs
further
enhance
probe's
responsive
performance
extend
wavelength.
Upon
AChE‐activization,
undergoes
deionization,
molecule
aggregation
increased
,
triggering
amplified
signal.
has
successfully
realized
monitoring
endogenous
PC12
cells,
tissues
living
mice.
Furthermore,
utilizing
light‐sheet
microscopy,
intracranial
AChE,
providing
highly
specialized
visualization
localization
within
brain,
achieved
first
time.
This
study
satisfactorily
demonstrates
valuable
designing
ultra‐sensitive
enzymatic
probes,
highlighting
their
potential
precise
analysis
fundamental
life
science
research.
Journal of the American Chemical Society,
Год журнала:
2022,
Номер
144(23), С. 10647 - 10658
Опубликована: Май 31, 2022
Delivery
of
functional
proteins
into
the
intracellular
space
has
been
a
challenging
task
that
could
lead
to
myriad
therapeutic
applications.
We
report
herein
novel
bioconjugation
strategy
for
enzyme
modification
and
selective
delivery
cancer
cells
lock-and-key-type
activation
photosensitizers.
Using
bifunctional
linker
containing
bis(bromomethyl)phenyl
group
an
o-phthalaldehyde
moiety,
it
induce
cyclization
peptide
sequence
Ac-NH-CRGDfC-CONH2
through
site-specific
dibenzylation
with
two
cysteine
residues
further
coupling
β-galactosidase
via
phthalaldehyde-amine
capture
reaction.
This
facile
two-step
one-pot
procedure
enabled
preparation
cyclic
RGD-modified
readily,
which
be
internalized
selectively
αvβ3
integrin-overexpressed
cells.
Upon
encountering
intrinsically
quenched
distyryl
boron
dipyrromethene-based
photosensitizer
conjugated
galactose
moiety
self-immolative
inside
cells,
extrinsic
induced
specific
cleavage
β-galactosidic
bond
followed
by
self-immolation
release
activated
derivative,
thereby
restoring
photodynamic
activities
causing
cell
death
effectively.
The
high
specificity
this
enzyme-activated
photosensitizing
system
was
also
demonstrated
in
vivo
using
nude
mice
bearing
integrin-positive
U87-MG
tumor.
at
tumor
site
resulted
lighting
up
complete
eradication
upon
laser
irradiation,
while
native
β-galactosidase,
effects
were
largely
reduced.
In
contrast
conventional
intrinsic
enzymes,
activatable
approach
can
minimize
nonspecific
toward
precisive
therapy.
Chemical & Biomedical Imaging,
Год журнала:
2023,
Номер
1(7), С. 590 - 619
Опубликована: Май 30, 2023
Among
the
recent
development
of
click
chemistry
and
bioorthogonal
chemistry,
fluorogenic
reactions
occupy
a
unique
place
in
that
fluorescence
is
generated
from
nonfluorescent
reactants,
thereby
rendering
them
highly
useful
convenient
no-wash
live-cell
imaging.
This
topic
was
extensively
reviewed
2010
by
Wang
et
al.
(
Chem.
Soc.
Rev.
2010,
39,
1233−1239)
2014
Lin
Curr.
Opin.
Biol.
2014,
21,
89−95).
review
presents
comprehensive
up-to-date
overview
on
past
decade.
The
are
classified
into
four
major
categories
basis
mechanisms
generation.
Representative
examples
each
type
discussed
briefly
terms
structure,
mechanism,
advantages.
We
describe
latest
applications
chemical
biology.
In
end,
future
opportunities
challenges
this
field
tentatively
proposed.