Enhancing the bioconversion rate and end products of black soldier fly (BSF) treatment – A comprehensive review

Environment Development and Sustainability, Год журнала: 2024, Номер unknown

Опубликована: Янв. 4, 2024

Abstract Food security remains a pressing concern in the face of an increasing world population and environmental challenges. As climate change, biodiversity loss, water scarcity continue to impact agricultural productivity, traditional livestock farming faces limitations meeting growing global demand for meat dairy products. In this context, black soldier fly larvae (BSFL) have emerged as promising alternative sustainable food production. BSFL possess several advantages over conventional livestock, including their rapid growth, adaptability various organic waste substrates, low impact. Their bioconversion rate, ability transform into valuable products, final product optimization are key factors that enhance potential nutrient-rich protein source, fertilizer, biofuel. This review explores strategies rate improve end products derived from BSF treatment. It highlights benefits using other interventions underscores significance optimizing meet challenges sustainably. Despite prospects BSF-derived consumer acceptance regulatory hurdles remain critical aspects address realizing full market potential. The utilization source feed can contribute management, reduce pollution, issue environmentally responsible manner. However, there is need further research innovation ensure safety, quality, economic viability BSF-based both animal human consumption.

Язык: Английский

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Bioconversion of Different Waste Streams of Animal and Vegetal Origin and Manure by Black Soldier Fly Larvae Hermetia illucens L. (Diptera: Stratiomyidae)

Insects, Год журнала: 2023, Номер 14(2), С. 204 - 204

Опубликована: Фев. 17, 2023

Black soldier fly larvae (BSFL) are considered a commercially viable solution for global organic waste problems. The objective of this study was to assess the feasibility rearing BSFL on wide range low-value streams and its potential transform them into high-quality animal feed fertilizer. Six different origins were selected each tested in triplicate. Several parameters analysed: growth performance, reduction index (WRI), conversion efficiency (ECI) larval composition. Frass composition also analysed. Larvae reared fast food (FFW) had highest ECI WRI lowest values when pig manure slurry mixed with silage grass (PMLSG) slaughter (SW). protein content found mushroom stems (MS) although substrate content. Moreover, frass nutritional profile proportionally related substrate's profile: protein-rich (SW) resulted low-protein protein-poor frass. same true lipid In conclusion, showed that can be successfully affect chemical compositions.

Язык: Английский

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In Vitro Evaluation of the Antibacterial Activity of the Peptide Fractions Extracted from the Hemolymph of Hermetia illucens (Diptera: Stratiomyidae)

Insects, Год журнала: 2023, Номер 14(5), С. 464 - 464

Опубликована: Май 15, 2023

Antimicrobial peptides (AMPs) are a chemically and structurally heterogeneous family of molecules produced by large variety living organisms, whose expression is predominant in the sites most exposed to microbial invasion. One richest natural sources AMPs insects which, over course their very long evolutionary history, have adapted numerous different habitats developing powerful innate immune system that has allowed them survive but also assert themselves new environment. Recently, due increase antibiotic-resistant bacterial strains, interest risen. In this work, we detected hemolymph Hermetia illucens (Diptera, Stratiomyidae) larvae, following infection with Escherichia coli (Gram negative) or Micrococcus flavus positive) from uninfected larvae. Peptide component, isolated via organic solvent precipitation, was analyzed microbiological techniques. Subsequent mass spectrometry analysis us specifically identify expressed basal condition differentially after challenge. We identified 33 all samples, which 13 stimulated Gram negative and/or positive mostly challenge could be responsible for more specific activity.

Язык: Английский

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Antimicrobial activity of lipids extracted from Hermetia illucens reared on different substrates

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Янв. 23, 2024

Abstract As the problem of antimicrobial resistance is constantly increasing, there a renewed interest in products derived from natural sources, particularly obtained innovative and eco-friendly materials. Insect lipids, due to their fatty acid composition, can be classified as compounds. In order assess antibacterial efficacy Hermetia illucens we extracted this component larval stage, fed on different substrates characterized it. Moreover, analyzed composition feeding substrate, determine if how it could affect activity lipid component. The was evaluated against Gram-positive Micrococcus flavus Gram-negative bacteria Escherichia coli . Analyzing profiles lipids that showed two bacterial strains, detected significant differences for C4:0, C10:0, C16:1, C18:3 n3 (ALA), C20:1. strongest verified by larvae reared strawberry, tangerine, fresh manure substrates, with growth inhibition zones ranged 1.38 1.51 mm, while only rearing effect Notably, profile H. seems not really influenced substrate profile, except C18:0 C18:2 CIS n6 (LA). This implies other factors, such conditions, development stages, nutrients carbohydrates, amount acids insects. Key points • Feeding influence (FA) Generally, no direct correlation between FAs same Specific more BSF Graphical abstract

Язык: Английский

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Larval Frass of Hermetia illucens as Organic Fertilizer: Composition and Beneficial Effects on Different Crops

Insects, Год журнала: 2024, Номер 15(4), С. 293 - 293

Опубликована: Апрель 20, 2024

Hermetia illucens has received a lot of attention as its larval stage can grow on organic substrates, even those that are decomposing. Black soldier fly breeding provides variety valuable products, including frass, mixture excrements, exuviae, and leftover feedstock, be used fertilizer in agriculture. Organic fertilizers, such bringing beneficial bacteria materials into the soil, improves health fertility. This comprehensive review delves comparative analysis frass derived from larvae fed different substrates. The composition micro- macro-nutrients, pH levels, matter content, electrical conductivity, moisture proportion dry under consideration. effect feeding substrates presence potentially for plant growth within is also reported. A critical feature examined this post-application impacts crops, highlighting agricultural benefits drawbacks introducing cultivation operations. One notable categorization crops studied distinct groups, which useful to simplify comparisons future research.

Язык: Английский

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Bioconversion of organic waste by insects – A comprehensive review

Process Safety and Environmental Protection, Год журнала: 2024, Номер 187, С. 1 - 25

Опубликована: Апрель 28, 2024

The ever-growing human population has forced people to intensify agriculture meet the demand for food and feed. However, increase in global agricultural production also means an waste generation, which is mostly improperly managed, especially low- middle-income countries where adequate management infrastructure limited inefficient. Consequently, organic become a burden due its negative impact on environment health. In this sense, insect-based bioconversion could be economically viable, environmentally friendly, technically feasible approach management. Based review, wastes from agriculture, industry, household, municipalities, starch industry are considered as suitable substrates bioconverter insects. Commonly used insects include cricket, yellow mealworm, superworm, black soldier fly, house flesh fly blow fly. Rearing facilities must adapted provide optimal environmental conditions growth development of reared insect maximize rate efficiency. Bioconverter their by-products can industrially processed into animal feed, fertilizer biodiesel or extracted isolate bioactive compounds. challenges associated with social acceptance, method-, infrastructure- technology-related problems, availability substrates, political legal regulations. summary, potential reduce waste, create jobs, novel products, offer sustainable source protein feed world's growing population.

Язык: Английский

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A novel family of defensin-like peptides from Hermetia illucens with antibacterial properties

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Май 16, 2024

Abstract Background The world faces a major infectious disease challenge. Interest in the discovery, design, or development of antimicrobial peptides (AMPs) as an alternative approach for treatment bacterial infections has increased. Insects are good source AMPs which main effector molecules their innate immune system. Black Soldier Fly Larvae (BSFL) being developed large-scale rearing food sustainability, waste reduction and sustainable animal fish feed. Bioinformatic studies have suggested that BSFL largest number identified insects. However, most BSF not yet undergone evaluation but promising leads to treat critical infections. Results Jg7197.t1, Jg7902.t1 Jg7904.t1 were expressed into haemolymph larvae following infection with Salmonella enterica serovar Typhimurium predicted be using computational tool ampir. genes encoding these proteins within 2 distinct clusters chromosome 1 genome. Following removal signal peptides, structures mature superimposed, highlighting high degree structural conservation. 3 share primary sequences contain Kunitz-binding domain; characterised inhibitory action against proteases, activities. An vitro screen indicated heterologously SUMO-Jg7197.t1 SUMO-Jg7902.t1 did show activity 12 strains. While recombinant SUMO-Jg7904.t1 had range Gram-negative Gram-positive bacteria, including serious pathogen Pseudomonas aeruginosa . Conclusions We cloned purified putative from performed initial experiments evaluate activity. In doing so, we novel defensin-like AMP, Jg7904.t1, encoded paralogous gene cluster, P. aeruginosa.

Язык: Английский

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Peptide Fractions Extracted from the Hemolymph of Hermetia illucens Inhibit Growth and Motility and Enhance the Effects of Traditional Chemotherapeutics in Human Colorectal Cancer Cells

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1891 - 1891

Опубликована: Фев. 22, 2025

Cancer is a leading cause of death worldwide, characterized by uncontrolled cell growth and multiple mutations. Chemotherapy often associated with harmful side effects, cancer cells may become resistant through various mechanisms. New approaches, which are able to address both the toxicity resistance issues chemotherapy, primary importance in research. Antimicrobial peptides (AMPs), naturally occurring molecules innate immune system all living organisms, have wide spectrum cytotoxic activities against could be promising alternative actual chemotherapeutics. Here, we tested peptide fractions, rich AMPs, extracted from hemolymph larvae insect Hermetia illucens on HT29 HCT116 human colorectal cells, observing inhibition accumulation G2/M phase increased apoptosis. Furthermore, extract induced significant cytoskeleton reorganization, resulting reduced motility. These effects were more evident fractions obtained Escherichia coli-infected larvae. The also enhanced traditional Overall, results suggest presence biologically active H. larvae, confirming that insect-derived research area oncology.

Язык: Английский

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Deciphering the functional diversity of the gut microbiota of the black soldier fly (Hermetia illucens): recent advances and future challenges

Animal Microbiome, Год журнала: 2023, Номер 5(1)

Опубликована: Авг. 31, 2023

Bioconversion using insects is a promising strategy to convert organic waste (catering leftovers, harvest waste, food processing byproducts, etc.) into biomass that can be used for multiple applications, turned high added-value products, and address environmental, societal economic concerns. Due its ability feed on tremendous variety of wastes, the black soldier fly (Hermetia illucens) has recently emerged as insect bioconversion wastes an industrial scale. A growing number studies have highlighted pivotal role gut microbiota in performance health this species. This review aims provide critical overview current knowledge regarding functional diversity H. illucens, highlighting importance bioconversion, safety development new biotechnological tools. After providing different strategies been outline microbial communities we discuss these microbes beneficial services they their host. Emphasis placed technical aspects host biology require special attention near future research. We also argue singular digestive capabilities complex illucens make species valuable model addressing fundamental questions interactions evolved with microorganisms. By proposing avenues research, stimulate research challenges but bacterial symbiosis insects.

Язык: Английский

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Preliminary investigation on the effect of insect-based chitosan on preservation of coated fresh cherry tomatoes

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Апрель 29, 2023

Abstract Chitosan was produced from Hermetia illucens pupal exuviae by heterogeneous and homogeneous deacetylation. Tomato fruits ( Solanum lycopersicum ), that are one of the most grown consumed food throughout world, were coated with 0.5 1% chitosan, applied dipping or spraying, stored at room temperature 4 °C, for a storage period 30 days. Statistical analysis give different results depending on analysed parameters: indeed, had better effect than homogenous in maintaining more stable physico-chemical parameters, while chitosan improved total phenols, flavonoids antioxidant activity. coatings spraying effective all analyses. derived H. always performed similarly to commercial chitosan. However, general performance insect-derived concentration phenolics flavonoids, activity observed as compared one. coating has already been successfully used preservation fresh fruits, alternative synthetic polymers, but this is first investigation an insect application. These preliminary encouraging regarding validation source

Язык: Английский

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