Hydrogel Drug Delivery Systems for Bone Regeneration

Pharmaceutics, Год журнала: 2023, Номер 15(5), С. 1334 - 1334

Опубликована: Апрель 25, 2023

With the in-depth understanding of bone regeneration mechanisms and development tissue engineering, a variety scaffold carrier materials with desirable physicochemical properties biological functions have recently emerged in field regeneration. Hydrogels are being increasingly used engineering because their biocompatibility, unique swelling properties, relative ease fabrication. Hydrogel drug delivery systems comprise cells, cytokines, an extracellular matrix, small molecule nucleotides, which different depending on chemical or physical cross-linking. Additionally, hydrogels can be designed for types specific applications. In this paper, we summarize recent research using as carriers, detail application defect diseases mechanisms, discuss future directions hydrogel engineering.

Язык: Английский

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Engineered biochemical cues of regenerative biomaterials to enhance endogenous stem/progenitor cells (ESPCs)-mediated articular cartilage repair

Bioactive Materials, Год журнала: 2023, Номер 26, С. 490 - 512

Опубликована: Май 2, 2023

As a highly specialized shock-absorbing connective tissue, articular cartilage (AC) has very limited self-repair capacity after traumatic injuries, posing heavy socioeconomic burden. Common clinical therapies for small- to medium-size focal AC defects are well-developed endogenous repair and cell-based strategies, including microfracture, mosaicplasty, autologous chondrocyte implantation (ACI), matrix-induced ACI (MACI). However, these treatments frequently result in mechanically inferior fibrocartilage, low cost-effectiveness, donor site morbidity, short-term durability. It prompts an urgent need innovative approaches pattern pro-regenerative microenvironment yield hyaline-like with similar biomechanical biochemical properties as healthy native AC. Acellular regenerative biomaterials can create favorable local environment without causing relevant regulatory scientific concerns from treatments. A deeper understanding of the mechanism healing is furthering (bio)design application scaffolds. Currently, utilization magnify repairing effect joint-resident stem/progenitor cells (ESPCs) presents evolving improvement repair. This review starts by briefly summarizing current vital roles ESPCs chemoattractants regeneration. Then several intrinsic hurdles biomaterials-based discussed. The recent advances novel regarding cues provide instructive extracellular guide (e.g. adhesion, migration, proliferation, differentiation, matrix production, remodeling) summarized. Finally, this outlines future directions engineering next-generation toward ultimate translation.

Язык: Английский

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3D Bioprintable Hypoxia-Mimicking PEG-Based Nano Bioink for Cartilage Tissue Engineering

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(16), С. 19921 - 19936

Опубликована: Апрель 14, 2023

As hypoxia plays a significant role in the formation and maintenance of cartilage tissue, aiming to develop native hypoxia-mimicking tissue engineering scaffolds is an efficient method treat articular (AC) defects. Cobalt (Co) documented for its hypoxic-inducing effects vitro by stabilizing hypoxia-inducible factor-1α (HIF-1α), chief regulator stem cell fate. Considering this, we developed novel three-dimensional (3D) bioprintable nano bioink wherein cobalt nanowires (Co NWs) were incorporated into poly(ethylene glycol) diacrylate (PEGDA) hydrogel system as hypoxia-inducing agent encapsulated with umbilical cord-derived mesenchymal cells (UMSCs). In current study, investigated impact Co NWs on chondrogenic differentiation UMSCs PEGDA system. Herein, (PEGDA+Co NW) was rheologically optimized bioprint geometrically stable cartilaginous constructs. The bioprinted 3D constructs evaluated their physicochemical characterization, swelling-degradation behavior, mechanical properties, proliferation, expression markers histological, immunofluorescence, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) methods. results disclosed that, compared control group, group outperformed print fidelity properties. Furthermore, live/dead staining, double-stranded DNA (dsDNA) content, glycosaminoglycans (GAGs) content demonstrated that adding low amounts (<20 ppm) supported UMSC adhesion, differentiation. Histological immunofluorescence staining PEGDA+Co NW structures revealed production type 2 collagen (COL2) sulfated GAGs, rendering it feasible option repair. It further corroborated upregulation hypoxia-mediated downregulation hypertrophic/osteogenic marker expression. conclusion, system, including Co2+ ions, synergistically directs toward chondrocyte lineage without using expensive growth factors provides alternative strategy translational applications field.

Язык: Английский

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Tissue regeneration properties of hydrogels derived from biological macromolecules: A review

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 271, С. 132280 - 132280

Опубликована: Май 13, 2024

Язык: Английский

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Synergistic large segmental bone repair by 3D printed bionic scaffolds and engineered ADSC nanovesicles: Towards an optimized regenerative microenvironment

Biomaterials, Год журнала: 2024, Номер 308, С. 122566 - 122566

Опубликована: Апрель 8, 2024

Achieving sufficient bone regeneration in large segmental defects is challenging, with the structure of repair scaffolds and their loaded bioactive substances crucial for modulating local osteogenic microenvironment. This study utilized digital laser processing (DLP)-based 3D printing technology to successfully fabricate high-precision methacryloylated polycaprolactone (PCLMA) bionic scaffold structures. Adipose-derived stem cell-engineered nanovesicles (ADSC-ENs) were uniformly stably modified onto surface using a perfusion device, constructing conducive microenvironment tissue long defect through scaffold's structural design vesicles' biological functions. Scanning electron microscopy (SEM) examination confirmed efficient loading ADSC-ENs. The material group vesicles (PCLMA-BAS-ENs) demonstrated good cell compatibility potential when analyzed adhesion osteogenesis primary rabbit marrow mesenchymal cells (BMSCs) on surface. Tested 15 mm critical radial model, PCLMA-BAS-ENs facilitated near-complete after 12 weeks. Immunofluorescence proteomic results indicated that significantly improved at site vivo, promoted angiogenesis, enhanced polarization macrophages towards M2 phenotype, recruitment BMSCs. Thus, was proven promote defects. Overall, this strategy combining engineered highly biomimetic large-segment holds great orthopedic other regenerative medicine applications.

Язык: Английский

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Enhanced ·OH‐Scavenging Activity of Cu‐CeO_x Nanozyme via Resurrecting Macrophage Nrf2 Transcriptional Activity Facilitates Diabetic Wound Healing

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(12)

Опубликована: Фев. 1, 2024

Diabetic wounds are a prevalent and devastating complication of diabetes, which may impede their healing regeneration. In diabetic wounds, excess reactive oxygen species (ROS) activate the nuclear factor kappa-B pathway, leading to transcriptional silencing erythroid 2-related 2 (Nrf2), resulting in vicious cycle oxidative stress inflammation. Conventional nanozymes have limitations preventing continuous production ROS, including most oxidizing hydroxyl radical (·OH), although they can remove pre-existing ROS. Herein, novel antioxidant nanoplatform addresses this challenge by incorporating JSH-23 into mesoporous cupric-doped cerium oxide nanozymes. Additionally, for rapid wound adaptability durable tissue adhesion, nanozyme hydrogel spray consisting oxidized sodium alginate methacrylate gelatin is constructed, named OG@CCJs. This platform resurrects Nrf2 activity macrophages vitro, curbing ROS at its source, particularly ·OH, while enabling scavenge previously generated OG@CCJs significantly alleviate vivo, promoting healing. Overall, proposed nanozyme-hydrogel with enhanced ·OH-scavenging uses "two-track" strategy rebuild defense barrier macrophages. pioneering approach highlights tremendous potential facilitating

Язык: Английский

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Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Biomedical Materials, Год журнала: 2024, Номер 19(4), С. 042008 - 042008

Опубликована: Май 20, 2024

Gelatin methacryloyl (GelMA) hydrogels have gained significant recognition as versatile biomaterials in the biomedical domain. GelMA emulate vital characteristics of innate extracellular matrix by integrating cell-adhering and metalloproteinase-responsive peptide motifs. These features enable cellular proliferation spreading within GelMA-based hydrogel scaffolds. Moreover, displays flexibility processing, it experiences crosslinking when exposed to light irradiation, supporting development with adjustable mechanical characteristics. The drug delivery landscape has been reshaped hydrogels, offering a favorable platform for controlled sustained release therapeutic actives. tunable physicochemical precise modulation kinetics release, ensuring optimal effectiveness. In tissue engineering, perform an essential role design scaffold, providing biomimetic environment conducive cell adhesion, proliferation, differentiation. Incorporating three-dimensional printing further improves its applicability developing complicated constructs spatial precision. Wound healing applications showcase bioactive dressings, fostering microenvironment regeneration. inherent biocompatibility provide efficiency closure wounds repair. stand at forefront innovation, addressing diverse challenges delivery, wound healing. This review provides comprehensive overview, in-depth understanding hydrogel's potential impact on progressing sciences.

Язык: Английский

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Identification and function of periosteal skeletal stem cells in skeletal development, homeostasis, and disease

Journal of Orthopaedic Translation, Год журнала: 2025, Номер 51, С. 177 - 186

Опубликована: Март 1, 2025

Язык: Английский

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Advancing drug delivery to articular cartilage: From single to multiple strategies

Acta Pharmaceutica Sinica B, Год журнала: 2022, Номер 13(10), С. 4127 - 4148

Опубликована: Ноя. 25, 2022

Язык: Английский

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3D bioprinted autologous bone particle scaffolds for cranioplasty promote bone regeneration with both implanted and native BMSCs

Biofabrication, Год журнала: 2023, Номер 15(2), С. 025016 - 025016

Опубликована: Фев. 22, 2023

Although autologous bone (AB) grafting is considered to be the gold standard for cranioplasty, unresolved problems remain, such as surgical-site infections and flap absorption. In this study, an AB scaffold was constructed via three-dimensional (3D) bedside-bioprinting technology used cranioplasty. To simulate skull structure, a polycaprolactone shell designed external lamina, 3D-printed marrow-derived mesenchymal stem cell (BMSC) hydrogel mimic cancellous regeneration. Ourin vitroresults showed that exhibited excellent cellular affinity promoted osteogenic differentiation of BMSCs in both two-dimensional 3D culture systems. The implanted beagle dog cranial defects up 9 months, new osteoid formation. Furtherin vivostudies indicated transplanted differentiated into vascular endothelium, cartilage, tissues, whereas native were recruited defect. results study provide method bedside bioprinting cranioplasty regeneration, which opens another window clinical applications printing future.

Язык: Английский

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