Potential Antimicrobial and Antibiofilm Properties of Copper Oxide Nanoparticles: Time-Kill Kinetic Essay and Ultrastructure of Pathogenic Bacterial Cells

Applied Biochemistry and Biotechnology, Год журнала: 2022, Номер 195(1), С. 467 - 485

Опубликована: Сен. 10, 2022

Abstract Mycosynthesis of nanoparticle (NP) production is a potential ecofriendly technology for large scale production. In the present study, copper oxide nanoparticles (CuONPs) have been synthesized from live cell filtrate fungus Penicillium chrysogenum . The created CuONPs were characterized via several techniques, namely Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscope (TEM), scanning (SEM), and energy-dispersive spectroscopy (EDX). Furthermore, biosynthesized performed against biofilm forming Klebsiella oxytoca ATCC 51,983, Escherichia coli 35,218, Staphylococcus aureus 25,923, Bacillus cereus 11,778. anti-bacterial activity result was shown with zone inhibition determined to be 14 ± 0.31 mm, 16 0.53 11 0.57 10 mm respectively. more susceptible minimal inhibitory concentration (MIC) values 6.25 3.12 µg/mL, respectively, while , MIC value 12.5 25 μg/mL, minimum (MBIC) evident, that excellent anti-biofilm at sub-MIC levels reducing formation by 49% 59% results indicated MBIC on higher than 200 μg/mL 256 suggesting these could not inhibit mature formatted in vitro. Overall, all clearly confirmed ability prepared offer smart approach biomedical therapy resistant microorganisms because its promoted antimicrobial action, but only specified purposes.

Язык: Английский

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Synthesis of Silver Nanocomposite Based on Carboxymethyl Cellulose: Antibacterial, Antifungal and Anticancer Activities

Polymers, Год журнала: 2022, Номер 14(16), С. 3352 - 3352

Опубликована: Авг. 17, 2022

Traditional cancer treatments include surgery, radiation, and chemotherapy. According to medical sources, chemotherapy is still the primary method for curing or treating today has been a major contributor recent decline in mortality. Nanocomposites based on polymers metal nanoparticles have recently received attention of researchers. In current study, nanocomposite was fabricated carboxymethyl cellulose silver (CMC-AgNPs) their antibacterial, antifungal, anticancer activities were evaluated. The antibacterial results revealed that CMC-AgNPs promising activity against Gram-negative (Klebsiella oxytoca Escherichia coli) Gram-positive bacteria (Bacillus cereus Staphylococcus aureus). Moreover, exhibited antifungal filamentous fungi such as Aspergillus fumigatus, A. niger, terreus. Concerning HepG2 hepatocellular cell line, lowest IC50 values (7.9 ± 0.41 µg/mL) recorded CMC-AgNPs, suggesting strong cytotoxic effect liver cells. As result, our findings suggest antitumor these CMC-Ag due induction apoptosis necrosis hepatic cells via increased caspase-8 -9 diminished levels VEGFR-2. conclusion, activities, which can be used pharmaceutical fields.

Язык: Английский

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Biosynthesis of Silver and Gold Nanoparticles and Their Efficacy Towards Antibacterial, Antibiofilm, Cytotoxicity, and Antioxidant Activities

Applied Biochemistry and Biotechnology, Год журнала: 2022, Номер 195(2), С. 1158 - 1183

Опубликована: Ноя. 7, 2022

The World Health Organization (WHO) reports that the emergence of multidrug-resistant and slow advent novel more potent antitumor antimicrobial chemotherapeutics continue to be highest concern for human health. Additionally, stability, low solubility, negative effects existing drugs make them ineffective. Studies into alternative tactics tackle such tenacious diseases was sparked by anticancer antibacterial. Silver (Ag) gold (Au) nanoparticles (NPs) were created from Trichoderma saturnisporum, much productive fungal strain. Functional extracellular enzymes proteins carried out activities synthesis capping generated nano-metals. Characterization done on obtained Ag-NPs Au-NPs through UV-vis, FTIR, XRD, TEM, SEM. versus methicillin-sensitive Staphylococcus aureus (MSSA) methicillin-resistant (MRSA), Pseudomonas aeruginosa, Klebsiella pneumoniae, antibacterial assessed. In particular, effective against pathogenic bacteria than Au-NPs. Furthermore, antibiofilm study shown had activity Ag-NPs. Interestingly, applying DPPH procedure, these noble metallic NPs antioxidant activity, in which IC50 73.5 μg/mL 190.0 μg/mL, respectively. According cytotoxicity evaluation results, alteration cells as loss their typical shape, partial or complete monolayer, granulation, shrinking, cell rounding with normal Vero 693.68 661.24 Au-NPs, While cancer (Mcf7) 370.56 394.79 produced via green have potential employed medical industry beneficial nanocompounds.

Язык: Английский

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Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria

Life, Год журнала: 2022, Номер 12(6), С. 893 - 893

Опубликована: Июнь 15, 2022

There is an increase of pathogenic multidrug-resistant bacteria globally due to the misuse antibiotics. Recently, more scientists used metal nanoparticles counteract antibacterial resistance. In this study, orange peel waste (OPW) was for selenium nanoparticles' (Se-NPs) biosynthesis through green and ecofriendly method, their applications as antibiofilm agents. Green biosynthesized Se-NPs were characterized using FTIR, XRD, SEM, EDAX, TEM. Characterization results revealed that highly crystalline, spherical, polydisperse, had sizes in range 16-95 nm. The evaluated activities against bacteria. Results illustrated exhibited potential activity Gram-positive (S. aureus ATCC 29213 biofilm-producing clinical isolates S. aureus) Gram-negative (Pseudomonas aeruginosa PAO1, MDR, biofilm, quorum-sensing producing MDR P. aeruginosa, E. coli, K. pneumonia). Moreover, most sensitive at 1000 µg/mL, where inhibition zone 35 mm MIC 25 µg/mL. Furthermore, 0.25 0.5 decreased biofilm significantly. largest noticed pneumonia, which 62% 92% MIC, respectively. conclusion, successfully OPW method promising bacteria, can be later fighting resistant

Язык: Английский

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Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Journal of Fungi, Год журнала: 2022, Номер 8(6), С. 612 - 612

Опубликована: Июнь 8, 2022

The purpose of this study is to create chitosan-stabilized silver nanoparticles (Chi/Ag-NPs) and determine whether they were cytotoxic also their characteristic antibacterial, antibiofilm, wound healing activities. Recently, the development an efficient environmentally friendly method for synthesizing metal based on polysaccharides has attracted a lot interest in field nanotechnology. Colloidal Chi/Ag-NPs are prepared by chemical reduction ions presence Chi, giving Chi/Ag-NPs. Physiochemical properties determined Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), scanning with energy-dispersive (SEM-EDX) analyses. TEM pictures indicate that generated nearly spherical shape thin chitosan covering around Ag core had sizes range 9-65 nm. In vitro antibacterial activity was evaluated against Staphylococcus aureus Pseudomonas aeruginosa resazurin-mediated microtiter plate assay. highest observed lowest concentration Chi/Ag-NPs, which 12.5 µg/mL both bacterial strains. Additionally, showed promising antifungal features Candida albicans, Aspergillus fumigatus, terreus, niger, where inhibition zones 22, 29, 20, 17 mm, respectively. Likewise, revealed potential antioxidant 92, 90, 75% at concentrations 4000, 2000, 1000 µg/mL, IC50 261 µg/mL. Wound results illustrated fibroblasts advanced toward opening close scratch roughly 50.5% after 24-h exposure greatly accelerating process. conclusion, nanocomposite AgNPs successfully exhibited antifungal, antioxidant, activities can be used medical field.

Язык: Английский

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Phytofabrication of zinc oxide nanoparticles with advanced characterization and its antioxidant, anticancer, and antimicrobial activity against pathogenic microorganisms

Biomass Conversion and Biorefinery, Год журнала: 2022, Номер 13(1), С. 417 - 430

Опубликована: Окт. 28, 2022

Язык: Английский

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Nanocomposite based on gold nanoparticles and carboxymethyl cellulose: Synthesis, characterization, antimicrobial, and anticancer activities

Journal of Drug Delivery Science and Technology, Год журнала: 2022, Номер 77, С. 103874 - 103874

Опубликована: Окт. 7, 2022

Язык: Английский

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Ecofriendly synthesis of silver nanoparticles using Kei-apple (Dovyalis caffra) fruit and their efficacy against cancer cells and clinical pathogenic microorganisms

Arabian Journal of Chemistry, Год журнала: 2022, Номер 15(7), С. 103927 - 103927

Опубликована: Апрель 21, 2022

Green fabrication has become a safe approach for producing nanoparticles. Plant-based biogenic synthesis of silver nanoparticles (AgNPs) emerged as possible alternative to traditional chemical production. In this paper, we provide low-cost, green AgNPs utilizing using Kei-apple (Dovyalis caffra) fruit extract. Ultraviolet–visible (UV–Vis) spectroscopy, Fourier Transform Infrared (FTIR), Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD), Scanning-Electron Microscope (SEM), and Dynamic Light Scattering (DLS) analyses were used characterize produced AgNPs. The formation was shown have surface resonance peak 415 nm in UV–visible spectra, FTIR spectra verified the participation biological molecules Synthesis TEM revealed that biosynthesized mostly spherical form, with size range 12–53 nm. XRD diffractogram demonstrate face cubic centre (fcc) character Excellent anticancer activity recorded where more than 80% Prostate Cancer (PC-3) cell lines inhibited by 100–150 µg/mL AgNPs, while 38% only AgNO3 55.62% D. caffra extract at 150 µg/mL. Destructions PC-3 observed result exposed followed extract, minor alterations AgNO3. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging three fold 100 indicating good antioxidant activity. inhibitory against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans Aspergillus fumigatus inhibition diameter zone 28.22 ± 0.25 mm, 23.21 0.35 27.25 0.03 28.40 0.15 29.23 0.44 9.52 0.5 respectively compared fruits considered promising source multi-biological functions.

Язык: Английский

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Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Lawsonia inermis Against Common Pathogens from Urinary Tract Infection

Applied Biochemistry and Biotechnology, Год журнала: 2023, Номер 196(1), С. 85 - 98

Опубликована: Апрель 26, 2023

Abstract New and creative methodologies for the fabrication of silver nanoparticles (Ag-NPs), which are exploited in a wide range consumer items, significant interest. Hence, this research emphasizes biological approach Ag-NPs through Egyptian henna leaves ( Lawsonia inermis Linn.) extracts analysis prepared Ag-NPs. Plant extract components were identified by gas chromatography mass spectrometry (GC-mass). The analyses carried out UV–visible (UV–Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning (SEM), Fourier transform infrared (FTIR) analysis. UV–Vis reveals that have maximum peak at 460 nm visible light. Structural characterization recorded peaks corresponded to Bragg’s diffractions nano-crystal, with average crystallite sizes varying from 28 60 nm. Antibacterial activities examined, it is observed all microorganisms very sensitive biologically synthesized

Язык: Английский

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Baker’s Yeast-Mediated Silver Nanoparticles: Characterisation and Antimicrobial Biogenic Tool for Suppressing Pathogenic Microbes

BioNanoScience, Год журнала: 2022, Номер 12(4), С. 1220 - 1229

Опубликована: Авг. 18, 2022

Язык: Английский

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