Cancers,
Год журнала:
2024,
Номер
16(11), С. 1967 - 1967
Опубликована: Май 22, 2024
Lung
cancer
represents
the
leading
cause
of
cancer-related
mortality
worldwide,
with
around
1.8
million
deaths
in
2020.
For
this
reason,
there
is
an
enormous
interest
finding
early
diagnostic
tools
and
novel
therapeutic
approaches,
one
which
extracellular
vesicles
(EVs).
EVs
are
nanoscale
membranous
particles
that
can
carry
proteins,
lipids,
nucleic
acids
(DNA
RNA),
mediating
various
biological
processes,
especially
cell–cell
communication.
As
such,
they
represent
interesting
biomarker
for
analysis
be
performed
easily
by
liquid
biopsy.
Moreover,
their
growing
dataset
shows
promising
results
as
drug
delivery
cargo.
The
aim
our
work
to
summarize
recent
advances
possible
implications
diagnosis
innovative
therapies
lung
cancer.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Янв. 28, 2024
Malignancy
is
a
major
public
health
problem
and
among
the
leading
lethal
diseases
worldwide.
Although
current
tumor
treatment
methods
have
therapeutic
effect
to
certain
extent,
they
still
some
shortcomings
such
as
poor
water
solubility,
short
half-life,
local
systemic
toxicity.
Therefore,
how
deliver
agent
so
realize
safe
effective
anti-tumor
therapy
become
urgently
be
solved
in
this
field.
As
medium
of
information
exchange
material
transport
between
cells,
exosomes
are
considered
promising
drug
delivery
carrier
due
their
nano-size,
good
biocompatibility,
natural
targeting,
easy
modification.
In
review,
we
summarize
recent
advances
isolation,
identification,
loading,
modification
carriers
for
alongside
application
therapy.
Basic
knowledge
exosomes,
biogenesis,
sources,
characterization
methods,
also
introduced
herein.
addition,
challenges
related
use
vehicles
discussed,
along
with
future
trends.
This
review
provides
scientific
basis
exosome
systems
oncological
ACS Nano,
Год журнала:
2024,
Номер
18(10), С. 7504 - 7520
Опубликована: Фев. 27, 2024
The
essential
role
of
the
neural
network
in
enhancing
bone
regeneration
has
often
been
overlooked
biomaterial
design,
leading
to
delayed
or
compromised
healing.
Engineered
mesenchymal
stem
cells
(MSCs)-derived
exosomes
are
becoming
increasingly
recognized
as
potent
cell-free
agents
for
manipulating
cellular
behavior
and
improving
therapeutic
effectiveness.
Herein,
MSCs
stimulated
with
nerve
growth
factor
(NGF)
regulate
exosomal
cargoes
improve
neuro-promotive
potential
facilitate
innervated
regeneration.
In
vitro
cell
experiments
showed
that
NGF-stimulated
MSCs-derived
(N-Exos)
obviously
improved
function
neurotrophic
effects
cells,
consequently,
osteogenic
osteo-reparative
cells.
Bioinformatic
analysis
by
miRNA
sequencing
pathway
enrichment
revealed
beneficial
N-Exos
may
partly
be
ascribed
NGF-elicited
multicomponent
miRNAs
subsequent
regulation
activation
MAPK
PI3K-Akt
signaling
pathways.
On
this
basis,
were
delivered
on
micropores
3D-printed
hierarchical
porous
scaffold
accomplish
sustained
release
profile
extended
bioavailability.
a
rat
model
distal
femoral
defect,
N-Exos-functionalized
significantly
induced
neurovascular
structure
formation
This
study
provided
feasible
strategy
modulate
functional
acquire
desirable
potential.
Furthermore,
developed
represent
promising
neurovascular-promotive
reparative
clinical
translation.
Frontiers in Cell and Developmental Biology,
Год журнала:
2022,
Номер
9
Опубликована: Янв. 14, 2022
Cellular
therapy
aims
to
replace
damaged
resident
cells
by
restoring
cellular
and
molecular
environments
suitable
for
tissue
repair
regeneration.
Among
several
candidates,
mesenchymal
stem/stromal
(MSCs)
represent
a
critical
component
of
stromal
niches
known
be
involved
in
homeostasis.
In
vitro
,
MSCs
appear
as
fibroblast-like
plastic
adherent
regardless
the
source.
The
therapeutic
value
is
being
explored
conditions,
including
immunological,
inflammatory
degenerative
diseases,
well
cancer.
An
improved
understanding
their
origin
function
would
facilitate
clinical
use.
stemness
still
debated
requires
further
study.
Several
terms
have
been
used
designate
MSCs,
although
consensual
nomenclature
has
yet
determined.
presence
distinct
markers
may
identification
isolation
specific
subpopulations
MSCs.
Regarding
properties,
mechanisms
underlying
immune
trophic
effects
imply
secretion
various
mediators
rather
than
direct
contact.
These
can
packaged
extracellular
vesicles,
thus
paving
way
exploit
cell-free
products
derived
from
Of
importance,
secretome
are
significantly
sensitive
environment.
features,
such
culture
delivery
method,
dose
immunobiology
influence
outcomes.
this
review,
we
will
summarize
recent
findings
related
MSC
properties.
We
also
discuss
main
preclinical
challenges
that
some
optimization
strategies.
Stem Cells International,
Год журнала:
2022,
Номер
2022, С. 1 - 24
Опубликована: Март 26, 2022
Hypoxia-mimetic
agents
are
new
potential
tools
in
MSC
priming
instead
of
hypoxia
incubators
or
chambers.
Several
pharmaceutical/chemical
hypoxia-mimetic
can
be
used
to
induce
the
tissues:
deferoxamine
(DFO),
dimethyloxaloylglycine
(DMOG),
2,4-dinitrophenol
(DNP),
cobalt
chloride
(CoCl2),
and
isoflurane
(ISO).
increase
cell
proliferation,
preserve
enhance
differentiation
potential,
migration
neovascularization
a
concentration-
stem
source-dependent
manner.
Moreover,
may
HIF-1α,
changing
metabolism
enhancing
glycolysis
like
hypoxia.
So,
there
is
clear
evidence
that
treatment
with
beneficial
regenerative
medicine,
preserving
capacities.
These
not
studied
so
wildly
as
but,
considering
low
cost
ease
use,
believed
find
application
pretreatment
many
diseases
such
ischemic
heart
disease
myocardial
fibrosis
promote
cardiac
cartilage
regeneration.
The
knowledge
critical
evaluating
safety
procedures
use
clinics.
In
this
review,
similarities
differences
between
terms
their
therapeutic
efficiency
considered
detail.
advantages,
challenges,
future
perspectives
mimetic
also
discussed.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(2), С. 1277 - 1277
Опубликована: Янв. 9, 2023
Mesenchymal
stem
cells
(MSCs)
with
self-renewing,
multilineage
differentiation
and
immunomodulatory
properties,
have
been
extensively
studied
in
the
field
of
regenerative
medicine
proved
to
significant
therapeutic
potential
many
different
pathological
conditions.
The
role
MSCs
mainly
depends
on
their
paracrine
components,
namely
secretome.
However,
components
MSC-derived
secretome
are
not
constant
affected
by
stimulation
exposed
to.
Therefore,
content
composition
can
be
regulated
pretreatment
MSCs.
We
summarize
effects
pretreatments
secretome,
focusing
order
provide
new
insights
for
application
inflammatory
immune
diseases.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(4), С. 3376 - 3376
Опубликована: Фев. 8, 2023
Lung
macrophages
(Mφs)
are
essential
for
pulmonary
innate
immunity
and
host
defense
due
to
their
dynamic
polarization
phenotype
shifts.
Mesenchymal
stromal
cells
(MSCs)
have
secretory,
immunomodulatory,
tissue-reparative
properties
shown
promise
in
acute
chronic
inflammatory
lung
diseases
COVID-19.
Many
beneficial
effects
of
MSCs
mediated
through
interaction
with
resident
alveolar
interstitial
Mφs.
Bidirectional
MSC-Mφ
communication
is
achieved
direct
contact,
soluble
factor
secretion/activation,
organelle
transfer.
The
microenvironment
facilitates
MSC
secretion
factors
that
result
Mφ
towards
an
immunosuppressive
M2-like
the
restoration
tissue
homeostasis.
turn
can
affect
immune
regulatory
function
engraftment
reparatory
effects.
This
review
article
highlights
mechanisms
crosstalk
between
Mφs
potential
role
repair
diseases.
Frontiers in Medicine,
Год журнала:
2024,
Номер
11
Опубликована: Фев. 29, 2024
Bone
defects
resulting
from
severe
trauma,
tumors,
inflammation,
and
other
factors
are
increasingly
prevalent.
Stem
cell-based
therapies
have
emerged
as
a
promising
alternative.
Dental
pulp
stem
cells
(DPSCs),
sourced
dental
pulp,
garnered
significant
attention
owing
to
their
ready
accessibility
minimal
collection-associated
risks.
Ongoing
investigations
into
DPSCs
revealed
potential
undergo
osteogenic
differentiation
capacity
secrete
diverse
array
of
ontogenetic
components,
such
extracellular
vesicles
cell
lysates.
This
comprehensive
review
article
aims
provide
an
in-depth
analysis
secretory
emphasizing
extraction
techniques
utilization
while
elucidating
the
intricate
mechanisms
governing
bone
regeneration.
Furthermore,
we
explore
merits
demerits
cell-free
therapeutic
modalities,
well
discuss
prospects,
opportunities,
inherent
challenges
associated
with
DPSC
therapy
in
context
Osteoarthritis
(OA)
presents
a
significant
therapeutic
challenge,
with
few
options
for
preserving
joint
cartilage
and
repairing
associated
tissue
damage.
Inflammation
is
pivotal
factor
in
OA-induced
deterioration
synovial
inflammation.
Recently,
exosomes
derived
from
human
umbilical
cord
mesenchymal
stem
cells
(HucMSCs)
have
gained
recognition
as
promising
noncellular
modality,
but
their
use
hindered
by
the
challenge
of
harvesting
sufficient
number
effective
efficacy.
Given
that
HucMSCs
are
highly
sensitive
to
microenvironmental
signals,
we
hypothesized
priming
within
proinflammatory
environment
would
increase
secreted
enhanced
anti-inflammatory
properties.
Subsequent
miRNA
profiling
pathway
analysis
confirmed
interleukin-1
beta
(IL-1β)-induced
(C-Exos)
exert
positive
effects
through
regulation
signaling
modulation.
In
vitro
experiments
revealed
C-Exos
enhance
chondrocyte
functionality
matrix
production,
well
macrophage
polarization,
thereby
enhancing
repair.
were
encapsulated
hyaluronic
acid
hydrogel
microspheres
(HMs)
ensure
sustained
release,
leading
substantial
improvements
inflammatory
microenvironment
regeneration
rat
OA
model.
This
study
outlines
strategy
tailor
exosome
cargo
regenerative
purposes,
functionalized
HMs
demonstrating
potential
treatment.
