Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair DOI Creative Commons

Bing‐Hui Jin,

Yiling Yu,

Chenghao Lou

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 10(4)

Published: Dec. 9, 2022

Peripheral nerve injury is a serious medical problem with limited surgical and clinical treatment options. It of great significance to integrate multiple guidance cues in one platform conduits (NGCs) promote axonal elongation functional recovery. Here, multi-functional NGC constructed regeneration by combining ordered topological structure, density gradient biomacromolecular nanoparticles, controlled delivery biological effectors provide the topographical, haptotactic, cues, respectively. On surface aligned polycaprolactone nanofibers, bioactive nanoparticles capable delivering recombinant human acidic fibroblast growth factor deposited. graded scaffold, proliferation Schwann cells promoted, directional extension neurites from both PC12 dorsal root ganglions improved direction increasing particle density. After being implanted vivo for 6 12 weeks repair 10-mm rat sciatic defect, promotes remyelination, achieving not only anatomical structure but also Taken together, provides favorable microenvironment peripheral holds promise realizing an efficacy close autograft.

Language: Английский

Emerging polymeric electrospun fibers: From structural diversity to application in flexible bioelectronics and tissue engineering DOI Creative Commons

Xingyi Wan,

Yunchao Zhao, Zhou Li

et al.

Exploration, Journal Year: 2022, Volume and Issue: 2(1)

Published: Jan. 28, 2022

Electrospinning (e-spin) technique has emerged as a versatile and feasible pathway for constructing diverse polymeric fabric structures, which show potential applications in many biological biomedical fields. Owing to the advantages of adjustable mechanics, designable surface multi-functionalization, biomimetic capability natural tissue, remarkable progress been made flexible bioelectronics tissue engineering sensing therapeutic purposes. In this perspective, we review recent works on design hierarchically structured e-spin fibers, well as, fabrication strategies from one-dimensional individual fiber (1D) three-dimensional (3D) arrangements adaptive specific applications. Then, focus most cutting-edge their engineering. Finally, propose future challenges perspectives promoting electrospun fiber-based products toward industrialized, intelligent, multifunctional, safe

Language: Английский

Citations

147
Anisotropic scaffolds for peripheral nerve and spinal cord regeneration DOI Creative Commons
Wen Xue, Wen Shi, Yunfan Kong

et al.

Bioactive Materials, Journal Year: 2021, Volume and Issue: 6(11), P. 4141 - 4160

Published: April 23, 2021

The treatment of long-gap (>10 mm) peripheral nerve injury (PNI) and spinal cord (SCI) remains a continuous challenge due to limited native tissue regeneration capabilities. current clinical strategy using autografts for PNI suffers from source shortage, while the pharmacological SCI presents dissatisfactory results. Tissue engineering, as an alternative, is promising approach regenerating nerves cords. Through providing beneficial environment, scaffold primary element in engineering. In particular, scaffolds with anisotropic structures resembling extracellular matrix (ECM) can effectively guide neural outgrowth reconnection. this review, anatomy cords, well treatments SCI, first summarized. An overview critical components engineering status approaches are also discussed. Recent advances fabrication surface patterns, aligned fibrous substrates, 3D hydrogel scaffolds, their vitro vivo effects highlighted. Finally, we summarize potential mechanisms underlying architectures orienting axonal glial cell growth, along challenges prospects.

Language: Английский

Citations

117
Electrospun Fibers Control Drug Delivery for Tissue Regeneration and Cancer Therapy DOI Creative Commons
Longfei Li,

Ruinan Hao,

Junjie Qin

et al.

Advanced Fiber Materials, Journal Year: 2022, Volume and Issue: 4(6), P. 1375 - 1413

Published: Sept. 16, 2022

Abstract Versatile strategies have been developed to construct electrospun fiber-based drug delivery systems for tissue regeneration and cancer therapy. We first introduce the construction of fiber scaffolds their various structures, as well commonly used types drugs. Then, we discuss some representative controlling by fibers, with specific emphasis on design endogenous external stimuli-responsive systems. Afterwards, summarize recent progress engineering, including soft engineering (such skin, nerve, cardiac repair) hard bone, cartilage, musculoskeletal systems), Furthermore, provide future development directions challenges facing use fibers controlled delivery, aiming insights perspectives smart platforms improve clinical therapeutic effects in Graphical abstract

Language: Английский

Citations

100
Neural tissue engineering: From bioactive scaffolds and in situ monitoring to regeneration DOI Creative Commons

Bowen Gong,

Xindan Zhang, Ahmed Al Zahrani

et al.

Exploration, Journal Year: 2022, Volume and Issue: 2(3)

Published: April 16, 2022

Peripheral nerve injury is a large-scale problem that annually affects more than several millions of people all over the world. It remains great challenge to effectively repair defects. Tissue engineered guidance conduits (NGCs) provide promising platform for peripheral through integration bioactive scaffolds, biological effectors, and cellular components. Herein, we firstly describe pathogenesis injuries at different orders severity clarify their microenvironments discuss clinical treatment methods challenges. Then, recent progress on design construction NGCs in combination with effectors components repair. Afterward, give perspectives imaging and/or conduit allow situ monitoring regeneration process. We also cover applications postoperative intervention treatments, such as electric field, magnetic light, ultrasound, well-designed improving efficacy. Finally, explore prospects multifunctional platforms promote injury.

Language: Английский

Citations

73
Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury DOI
Weidong Wu, Shuaijun Jia, Hailiang Xu

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(4), P. 3818 - 3837

Published: Feb. 14, 2023

Neural stem cells (NSCs) are considered to be prospective replacements for neuronal cell loss as a result of spinal cord injury (SCI). However, the survival and differentiation NSCs strongly affected by unfavorable microenvironment induced SCI, which critically impairs their therapeutic ability treat SCI. Herein, strategy fabricate PDGF-MP hydrogel (PDGF-MPH) microspheres (PDGF-MPHM) instead bulk hydrogels is proposed dramatically enhance efficiency platelet-derived growth factor mimetic peptide (PDGF-MP) in activating its receptor. PDGF-MPHM were fabricated piezoelectric ceramic-driven thermal electrospray device, had an average size 9 μm, also activate PDGFRβ more effectively than PDGF-MPH. In vitro, exerted strong neuroprotective effects maintaining proliferation inhibiting apoptosis presence myelin extracts. vivo, inhibited M1 macrophage infiltration extrinsic or intrinsic on seventh day after Eight weeks T10 SCI treatment results showed that + significantly promoted differentiation, reduced lesion size, considerably improved motor function recovery rats stimulating axonal regeneration, synapse formation, angiogenesis comparison with graft group. Therefore, our findings provide insights into promising agent repair.

Language: Английский

Citations

51
Bionic microenvironment-inspired synergistic effect of anisotropic micro-nanocomposite topology and biology cues on peripheral nerve regeneration DOI Creative Commons
Guicai Li, Tiantian Zheng, Linliang Wu

et al.

Science Advances, Journal Year: 2021, Volume and Issue: 7(28)

Published: July 7, 2021

Anisotropic micro-nanocomposite topographies and biological cues can synergistically promote peripheral nerve regeneration.

Language: Английский

Citations

81
Electrospinning nanofibers to 1D, 2D, and 3D scaffolds and their biomedical applications DOI

Huiling Zhong,

Jun Huang, Jun Wu

et al.

Nano Research, Journal Year: 2021, Volume and Issue: 15(2), P. 787 - 804

Published: Aug. 7, 2021

Language: Английский

Citations

77
Piezoelectric conduit combined with multi-channel conductive scaffold for peripheral nerve regeneration DOI
Ying Ma, Hao Wang, Qiqi Wang

et al.

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 452, P. 139424 - 139424

Published: Sept. 25, 2022

Language: Английский

Citations

61
Aligned fibrin/functionalized self-assembling peptide interpenetrating nanofiber hydrogel presenting multi-cues promotes peripheral nerve functional recovery DOI Creative Commons
Shuhui Yang, Jinjin Zhu,

Changfeng Lu

et al.

Bioactive Materials, Journal Year: 2021, Volume and Issue: 8, P. 529 - 544

Published: June 12, 2021

Nerve guidance conduits with hollow lumen fail to regenerate critical-sized peripheral nerve defects (15 mm in rats and 25 humans), which can be improved by a beneficial intraluminal microenvironment. However, individual cues provided filling materials are inadequate eliminate the functional gap between regenerated nerves normal nerves. Herein, an aligned fibrin/functionalized self-assembling peptide (AFG/fSAP) interpenetrating nanofiber hydrogel that exerting synergistic topographical biochemical for regeneration is constructed via electrospinning molecular self-assembly. The possesses structure, high water content, appropriate mechanical properties suitable biodegradation capabilities repair, enhances alignment neurotrophin secretion of primary Schwann cells (SCs) vitro, successfully bridges 15-mm sciatic vivo. transplanted AFG/fSAP exhibit satisfactory morphological recovery myelinated fibers innervated muscles. motor function facilitated comparable autografts. Moreover, upregulates regeneration-associated gene expression activates PI3K/Akt MAPK signaling pathways nerve. Altogether, represents promising approach repair through integration structural stimulation.

Language: Английский

Citations

59
Biomechanical microenvironment in peripheral nerve regeneration: from pathophysiological understanding to tissue engineering development DOI Creative Commons
Lingchi Kong, Xin Gao, Yun Qian

et al.

Theranostics, Journal Year: 2022, Volume and Issue: 12(11), P. 4993 - 5014

Published: Jan. 1, 2022

Peripheral nerve injury (PNI) caused by trauma, chronic disease and other factors may lead to partial or complete loss of sensory, motor autonomic functions, as well neuropathic pain.Biological activities are always accompanied mechanical stimulation, biomechanical microenvironmental homeostasis plays a complicated role in tissue repair regeneration.Recent studies have focused on the effects microenvironment peripheral nervous system development function maintenance, neural regrowth following PNI.For example, factors-induced cluster gene expression changes contribute formation structure maintenance physiological function.In addition, extracellular matrix cell responses alterations after PNI directly trigger series cascades for well-organized regeneration (PNR) process, where adhesion molecules, cytoskeletons mechanically gated ion channels serve mechanosensitive units, effector including focal kinase (FAK) yes-associated protein (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ) mechanotransduction elements.With rapid engineering techniques, substantial number PNR strategies such aligned guidance conduits, three-dimensional topological designs piezoelectric scaffolds emerge expected improve case PNI.These grafts display optimized properties outstanding mechanomodulatory effects, but few bottlenecks restrict their application scenes.In this review, current understanding associated is integrated, we proposed importance balances elements, cytoskeletal structures, cascades, components; wide variety promising based modulation introduced some suggestions prospects future directions.

Language: Английский

Citations

49