CuCo₂O₄ Nanoflowers with Multiple Enzyme Activities for Treating Bacterium-Infected Wounds via Cuproptosis-like Death

ACS Nano, Journal Year: 2024, Volume and Issue: 18(24), P. 15845 - 15863

Published: June 4, 2024

Nanozyme-driven catalytic therapy provides a promising treatment strategy for bacterial biofilm-infected wounds. However, the single functionality and limited efficiency of nanozyme-based materials often restrict effectiveness wound infection treatment. In this study, CuCo2O4 nanoflowers with multiple enzymatic activities were prepared antibacterial/antibiofilm by cuproptosis-like death. exhibited peroxidase-like (POD-like) oxidase-like (OXD-like) dual enzyme that generated large amounts •OH O2•–. Moreover, glutathione (GSH-Px-like) activity was able to reduce overexpression GSH in microenvironment, enhancing therapeutic effects reactive oxygen species (ROS). The morphology modified using hydrothermal method PEG4000 as solvent, resulting exposure more active center sites significant improvement activity. vitro results demonstrated pronounced disruption effect on biofilms formed bacteria. vivo, significantly promoted angiogenesis, collagen deposition, cell proliferation. Transcriptome sequencing revealed elevated ROS levels bacteria led membrane damage metabolic disruption. addition, Cu2+ overload induces lipid peroxidation accumulation disrupts respiratory chain tricarboxylic acid (TCA) cycle, ultimately leading This strategy, which combines synergistic enzyme-like death, an approach treating biofilm infections.

Language: Английский

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Natural Enzyme-Inspired Design of the Single-Atom Cu Nanozyme as Dual-Enzyme Mimics for Distinguishing Total Antioxidant Capacity and the Ascorbic Acid Level

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 14, 2024

Although various oxidase mimetic or peroxidase (POD) nanozymes have been extensively studied, their poor substrate selectivity significantly inhibits practical applications. Nanozymes with specific biomolecules as substrates, especially ascorbic acid (AAO) (AA) a substrate, scarcely studied. Herein, inspired by the multi-Cu atom sites and redox electron transfer pathway of Cu2+/Cu+ in natural AAO, atomically dispersed Cu immobilized on N-doped porous carbon (Cu-N/C) are artificially designed to simulate function AAO. Compared counterparts, Cu-N/C catalysts exhibited higher catalytic efficiency superior stability. Combined theoretical calculation experimental characterizations reveal that could catalyze AA oxidation through 2e– oxygen reduction H2O2 product. Moreover, also possess high POD activity. As proof-of-concept application, can simultaneously realize detection fluorescent mode based its AAO activity total antioxidant capacity colorimetric utilizing

Language: Английский

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A novel three-stage continuous sensing platform for H2O2 and cholesterol based on CuFeS2 nanozyme: Theoretical calculation and experimental verification

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 482, P. 148589 - 148589

Published: Jan. 21, 2024

Language: Английский

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Optimizing single-atom cerium nanozyme activity to function in a sequential catalytic system for colorimetric biosensing

Nano Today, Journal Year: 2024, Volume and Issue: 56, P. 102236 - 102236

Published: April 1, 2024

Language: Английский

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Enhancing radiation-resistance and peroxidase-like activity of single-atom copper nanozyme via local coordination manipulation

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 22, 2024

Abstract The inactivation of natural enzymes by radiation poses a great challenge to their applications for radiotherapy. Single-atom nanozymes (SAzymes) with high structural stability under such extreme conditions become promising candidate replacing shrink tumors. Here, we report CuN 3 -centered SAzyme (CuN -SAzyme) that exhibits higher peroxidase-like catalytic activity than 4 counterpart, locally regulating the coordination environment single copper sites. Density functional theory calculations reveal active moiety confers optimal H 2 O adsorption and dissociation properties, thus contributing enzymatic -SAzyme. introduction X-ray can improve kinetics decomposition Moreover, -SAzyme is very stable after total dose 500 Gy, without significant changes in its geometrical structure or environment, simultaneously still retains comparable relative enzymes. Finally, this developed remarkable radioresistance be used as an external field-improved therapeutics enhancing radio-enzymatic therapy vitro vivo. Overall, study provides paradigm developing SAzymes improved through local manipulation over enzymes, example, sensitizers cancer therapy.

Language: Английский

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Nanozyme engineering in structurally explicit framework: Design mechanisms and biosensing applications

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 500, P. 215517 - 215517

Published: Nov. 6, 2023

Language: Английский

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Piezoelectric BaTiO3 nanoparticles as oxidase mimics breaking pH limitation for colorimetric detection of glutathione reductase

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 481, P. 148609 - 148609

Published: Jan. 6, 2024

Language: Английский

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Lattice expansion in ruthenium nanozymes improves catalytic activity and electro-responsiveness for boosting cancer therapy

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 16, 2024

Nanozymes have been attracting widespread interest for the past decade, especially in field of cancer therapy, due to their intrinsic catalytic activities, strong stability, and ease synthesis. However, enhancing activity tumor microenvironment (TME) remains a major challenge. Herein, we manipulate activities Ru nanozymes via modulating lattice spacing nanocrystals supported on nitrogen-doped carbon support, achieve improvement multiple enzyme-like that can form cascade reactions boost cell killing. In addition, expansion improve responsiveness self-powered electric field, achieving maximized therapeutic outcome. Under electrical stimulation provided by human self-propelled triboelectric device, Ru-based nanozyme (Ru1000) with 5.99% realizes optimal performance outcome breast female tumor-bearing mice. Through theoretical calculations, uncover promote reaction, simultaneously, reducing electron density shifting d-band center active sites. This work provides opportunities improving development nanozymes.

Language: Английский

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Enhancing cancer treatment via “Zn2+ interference” with Zn-based nanomaterials

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 500, P. 215535 - 215535

Published: Nov. 3, 2023

Language: Английский

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Designing Bimetallic Metal‐Organic Framework‐Based Heterojunction Radiosensitizer for Enhanced Radiodynamic Therapy and Immunotherapy

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(11)

Published: Dec. 3, 2023

Abstract Radiotherapy, as one of the most common strategies for clinical cancer treatment, suffers from failure due to hypoxia, inflammation, and radiosensitivity difference malignant tumors, side effects, radioresistance. Heterojunction‐based radiosensitizers attract great attention lower excitation energy remarkable catalytic activity compared typical transition metal radiosensitizers. However, bio‐application heterojunction is in its infancy challenges structure engineering, rational band alignment, ideal photogenerated carrier migration. Herein, a bimetallic metal‐organic framework (MOF)‐based radiosensitizer reasonably designed enhance radiodynamic therapeutic efficiency Ru complex, activate natural killer (NK) cell‐mediated innate immune responses promoted tumor immunotherapy. This study not only designs novel MOF‐based but also elucidates underlying action mechanisms inhibiting proliferation, which sheds light on practicable design combination treatment.

Language: Английский

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