3D cell culture models in research: applications to lung cancer pharmacology DOI Creative Commons
Nathan Vella, Anthony G. Fenech,

Vanessa Petroni Magri

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

Lung cancer remains one of the leading causes cancer-related mortality worldwide, necessitating innovative research methodologies to improve treatment outcomes and develop novel strategies. The advent three-dimensional (3D) cell cultures has marked a significant advancement in lung research, offering more physiologically relevant model compared traditional two-dimensional (2D) cultures. This review elucidates various types 3D culture models currently used pharmacology, including spheroids, organoids engineered tissue models, having pivotal roles enhancing our understanding biology, facilitating drug development, advancing precision medicine. systems mimic complex spatial architecture microenvironment tumours, providing critical insights into cellular molecular mechanisms tumour progression, metastasis responses. Spheroids, derived from commercialized lines, effectively (TME), formation hypoxic nutrient gradients, crucial for evaluating penetration efficacy anti-cancer therapeutics. Organoids tumouroids, primary tissues, recapitulate heterogeneity cancers are instrumental personalized medicine approaches, supporting simulation vivo pharmacological responses patient-specific context. Moreover, these have been co-cultured with biomimicry extracellular matrix (ECM) components further heterotypic cell-cell cell-ECM interactions present within TME. significantly contributing identification therapeutic targets resistance against conventional therapies. Therefore, this summarizes latest findings involving together common laboratory-based assays study effects. Additionally, integration development workflows is discussed. accelerating translation laboratory clinical applications, thereby landscape treatment. By closely mirroring human not only enhance disease but also pave way effective

Language: Английский

New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic Assay DOI Creative Commons
Zhengpeng Wan, Marie Floryan, Mark F. Coughlin

et al.

Advanced Healthcare Materials, Journal Year: 2022, Volume and Issue: 12(14)

Published: Nov. 5, 2022

Abstract Previous studies have developed vascularized tumor spheroid models to demonstrate the impact of intravascular flow on progression and treatment. However, these not been widely adopted so vascularization spheroids in vitro is generally lower than tissues vivo. To improve level, a new strategy introduced form by adding fibroblasts (FBs) sequentially pre‐formed this method with cell lines from kidney, lung, ovary cancer. Tumor made higher FB densities periphery spheroid, which tend enhance vascularization. The vessels close are more perfusable ones other methods. Finally, chimeric antigen receptor (CAR) T cells perfused under continuous into immunotherapy evaluation using tumor‐on‐a‐chip model. This for establishing leads increased vitro, allowing examination immune, endothelial, stromal, responses static or conditions.

Language: Английский

Citations

46
From Organ-on-a-Chip to Human-on-a-Chip: A Review of Research Progress and Latest Applications DOI

Yisha Huang,

Tong Liu,

Qi Huang

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(7), P. 3466 - 3488

Published: July 11, 2024

Organ-on-a-Chip (OOC) technology, which emulates the physiological environment and functionality of human organs on a microfluidic chip, is undergoing significant technological advancements. Despite its rapid evolution, this technology also facing notable challenges, such as lack vascularization, development multiorgan-on-a-chip systems, replication body single chip. The progress has played crucial role in steering OOC toward mimicking microenvironment, including microenvironment replication, multiorgan microphysiological systems. Additionally, advancements detection, analysis, organoid imaging technologies have enhanced efficiency Organs-on-Chips (OOCs). In particular, integration artificial intelligence revolutionized imaging, significantly enhancing high-throughput drug screening. Consequently, review covers research Human-on-a-chip, sensors OOCs, latest applications biomedical field.

Language: Английский

Citations

11
Cancer-on-chip models for metastasis: importance of the tumor microenvironment DOI Creative Commons
Mohammad Jouybar, Charlotte M. de Winde, Katarina Wolf

et al.

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 42(4), P. 431 - 448

Published: Oct. 31, 2023

Cancer-on-chip (CoC) models, based on microfluidic chips harboring chambers for 3D tumor-cell culture, enable us to create a controlled tumor microenvironment (TME). CoC models are therefore increasingly used systematically study effects of the TME various steps in cancer metastasis. Moreover, have great potential developing novel therapies and predicting patient-specific response treatments. We review recent developments focusing three main components: (i) anisotropic extracellular matrix (ECM) architectures, (ii) vasculature, (iii) immune system. aim provide guidance biologists choose best approach addressing questions about role metastasis, inspire engineers develop technologies.

Language: Английский

Citations

21
Vascularized organoid-on-a-chip: design, imaging, and analysis DOI
Tingting Yu,

Qihang Yang,

Bo Peng

et al.

Angiogenesis, Journal Year: 2024, Volume and Issue: 27(2), P. 147 - 172

Published: Feb. 26, 2024

Language: Английский

Citations

8
High‐Scale 3D‐Bioprinting Platform for the Automated Production of Vascularized Organs‐on‐a‐Chip DOI Creative Commons
Anna Fritschen, Nils Lindner, Sebastian Scholpp

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(17)

Published: March 21, 2024

3D bioprinting possesses the potential to revolutionize contemporary methodologies for fabricating tissue models employed in pharmaceutical research and experimental investigations. This is enhanced by combining with advanced organs-on-a-chip (OOCs), which includes a complex arrangement of multiple cell types representing organ-specific cells, connective tissue, vasculature. However, both OOCs so far demand high degree manual intervention, thereby impeding efficiency inhibiting scalability meet technological requirements. Through combination drop-on-demand robotic handling microfluidic chips, print procedure achieved that proficient managing three distinct on chip within only minute, as well capable consecutively processing numerous without intervention. process rests upon development post-printing sealable chip, compatible different 3D-bioprinters easily connected perfusion system. The capabilities automized bioprint are showcased through creation multicellular vascularized liver carcinoma model chip. achieves full vascularization stable microvascular network formation over 14 days culture time, pronounced spheroidal growth albumin secretion HepG2 serving representative model.

Language: Английский

Citations

8
Advances in the Model Structure of In Vitro Vascularized Organ-on-a-Chip DOI Creative Commons

Hongze Yin,

Yue Wang, Na Liu

et al.

Cyborg and Bionic Systems, Journal Year: 2024, Volume and Issue: 5

Published: Jan. 1, 2024

Microvasculature plays a crucial role in human physiology and is closely related to various diseases. Building vitro vascular networks essential for studying tissue behavior with repeatable morphology signaling conditions. Engineered 3D microvascular network models, developed through advanced microfluidic-based techniques, provide accurate reproducible platforms the microvasculature vitro, an component designing organ-on-chips achieve greater biological relevance. By optimizing microstructure of microfluidic devices mimic vivo microenvironment, organ-specific models healthy pathological tissues can be created. This review summarizes recent advancements strategies constructing devices. It discusses static vascularization chips’ classification, structural characteristics, techniques used build them: growing blood vessels on chips either or dynamic, grown microchannels, elastic membranes, hydrogels. Finally, paper application scenarios key technical issues existing chips. also explores potential novel organoid chip approach that combines organoids organ generate better

Language: Английский

Citations

7
Microphysiological Systems as Organ-Specific In Vitro Vascular Models for Disease Modeling DOI Creative Commons
Ungsig Nam, Seokhun Lee, Ashfaq Ahmad

et al.

BioChip Journal, Journal Year: 2024, Volume and Issue: 18(3), P. 345 - 356

Published: May 14, 2024

Abstract The vascular system, essential for human physiology, is vital transporting nutrients, oxygen, and waste. Since structures are involved in various disease pathogeneses exhibit different morphologies depending on the organ, researchers have endeavored to develop organ-specific models. While animal models possess sophisticated morphologies, they significant discrepancies from tissues due species differences, which limits their applicability. To overcome limitations arising these oversimplification of 2D dish cultures, microphysiological systems (MPS) emerged as a promising alternative. These more accurately mimic microenvironment by incorporating cell interactions, physical stimuli, extracellular matrix components, thus facilitating enhanced tissue differentiation functionality. Importantly, MPS often utilize human-derived cells, greatly reducing disparities between model patient responses. This review focuses recent advancements MPS, particularly modeling discusses potential biological adaptation.

Language: Английский

Citations

7
Engineering next generation vascularized organoids DOI Creative Commons
Nicolas Werschler, Clément Quintard, Stéphanie Nguyen

et al.

Atherosclerosis, Journal Year: 2024, Volume and Issue: 398, P. 118529 - 118529

Published: July 1, 2024

Language: Английский

Citations

7
Vascularized tumor models for the evaluation of drug delivery systems: a paradigm shift DOI Creative Commons
Elliot Lopez, Claire Wilhelm, Teresa Simón‐Yarza

et al.

Drug Delivery and Translational Research, Journal Year: 2024, Volume and Issue: 14(8), P. 2216 - 2241

Published: April 15, 2024

Abstract As the conversion rate of preclinical studies for cancer treatment is low, user-friendly models that mimic pathological microenvironment and drug intake with high throughput are scarce. Animal key, but an alternative to reduce their use would be valuable. Vascularized tumor-on-chip combine great versatility scalable easy use. Several strategies integrate both tumor vascular compartments have been developed, few used assess delivery. Permeability, intra/extravasation, free circulation often evaluated, imperfectly recapitulate processes at stake. Indeed, targeting chemoresistance bypass must investigated design promising therapeutics. In vitro help development delivery systems (DDS) thus needed. They allow selecting good candidates before animal based on rational criteria such as accumulation, diffusion in tumor, potency, well absence side damage. this review, we focus vascularized models. First, detail fabrication, especially materials, cell types, coculture used. Then, different vascularization described along classical applications intra/extravasation or assessment. Finally, current trends DDS discussed overview efforts domain. Graphical

Language: Английский

Citations

6
Xenohormetic Phytochemicals Inhibit Neovascularization in Microphysiological Models of Vasculogenesis and Tumor Angiogenesis DOI Creative Commons

G. Wills Kpeli,

Kevin Conrad,

William Bralower

et al.

Advanced Biology, Journal Year: 2024, Volume and Issue: 8(7)

Published: June 4, 2024

Abstract Xenohormesis proposes that phytochemicals produced to combat stressors in the host plant exert biochemical effects animal cells lacking cognate receptors. Xenohormetic such as flavonoids and phytoalexins modulate a range of human cell signaling mechanisms but functional correlations with pathophysiology are lacking. Here, potent inhibitory grapefruit‐derived Naringenin (Nar) soybean‐derived Glyceollins (Gly) microphysiological models bulk tissue vasculogenesis tumor angiogenesis reported. Despite this interference vascular morphogenesis, Nar Gly not cytotoxic endothelial do prevent cycle entry. The anti‐vasculogenic Glyceollin significantly more sex‐matched female (XX) models. decrease viability or expression proangiogenic genes triple negative breast cancer (TNBC) spheroids, suggesting inhibition sprouting by MPS model microenvironment mediated via direct cells. study supports further research health‐promoting agents special attention action role biological sex, which can improve understanding dietary nutrition pharmacology phytochemical preparations.

Language: Английский

Citations

6