A novel approach for engineering DHCM/GelMA microgels: application in hepatocellular carcinoma cell encapsulation and chemoresistance research DOI Creative Commons
Dandan Zhou, Xiaoxiao Li, W F Liu

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: March 14, 2025

Liver cancer, a highly aggressive malignancy, continues to present significant challenges in therapeutic management due its pronounced chemoresistance. This resistance, which undermines the efficacy of conventional chemotherapy and targeted therapies, is driven by multifaceted mechanisms, with increasing emphasis placed on protective role tumor microenvironment (TME). The hepatocellular carcinoma extracellular matrix (ECM), primary non-cellular component TME, has emerged as critical regulator cancer progression drug particularly cell (HCC). In this study, hybrid biomimetic hydrogel was engineered integrating decellularized (DHCM) gelatin methacrylate (GelMA) precursors. composite DHCM/GelMA designed replicate physicochemical functional properties ECM, thereby offering platform explore interactions between HCCs their microenvironment. Leveraging custom-designed microfluidic 3D printing platform, we achieved high-throughput fabrication HCC-encapsulated microgels, characterized enhanced uniformity, biocompatibility, scalability. These microgels facilitated construction microtissues, were subsequently employed for chemoresistance studies. Our findings revealed that closely mimic microenvironment, effectively recapitulating key features ECM-mediated resistance. Mechanistic studies further demonstrated DHCM significantly upregulates expression Aquaporin 3 (AQP3) encapsulated HCCs. upregulation potentially activates mTOR signaling-associated autophagy pathways, enhancing models provide robust versatile studying underlying mechanisms resistance evaluating interventions. innovative approach highlights potential transformative tool cancer-associated tissue engineering anticancer screening. By enabling detailed investigations into ECM chemoresistance, study contributes advancing research offers promising strategies overcome ultimately improving clinical outcomes liver treatment.

Language: Английский

Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies DOI Creative Commons
Beatriz D. Cardoso, Elisabete M. S. Castanheira, S. Lanceros‐Méndez

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(18)

Published: March 11, 2023

Abstract The clinical translations of drugs and nanomedicines depend on coherent pharmaceutical research based biologically accurate screening approaches. Since establishing the 2D in vitro cell culture method, scientific community has improved cell‐based drug assays models. Those advances result more informative biochemical development 3D multicellular models to describe biological complexity better enhance simulation vivo microenvironment. Despite overall dominance conventional macroscopic methods, they present physicochemical operational challenges that impair scale‐up by not allowing a high parallelization, multidrug combination, high‐throughput screening. Their combination complementarity with microfluidic platforms enable microfluidics‐based unequivocal advantages therapies. Thus, this review presents an updated consolidated view miniaturization's physical, chemical, considerations scenario. It clarifies field using gradient‐based microfluidics, droplet‐based printed‐based digital‐based SlipChip, paper‐based microfluidics. Finally, it comparative analysis performance methods life achieve increased precision process.

Language: Английский

Citations

67
Bioactivity evaluation of Polygonum minus and identification of synergistic phytochemicals with 5-fluorouracil for colon cancer treatment DOI
Zhongming Yang, Zhongguo Yang, Md Zuki Abu Bakar

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: 210, P. 112917 - 112917

Published: Jan. 30, 2025

Language: Английский

Citations

2
Applications of Artificial Intelligence, Deep Learning, and Machine Learning to Support the Analysis of Microscopic Images of Cells and Tissues DOI Creative Commons
Muhammad Ali, Viviana Benfante,

Ghazal Basirinia

et al.

Journal of Imaging, Journal Year: 2025, Volume and Issue: 11(2), P. 59 - 59

Published: Feb. 15, 2025

Artificial intelligence (AI) transforms image data analysis across many biomedical fields, such as cell biology, radiology, pathology, cancer and immunology, with object detection, feature extraction, classification, segmentation applications. Advancements in deep learning (DL) research have been a critical factor advancing computer techniques for mining. A significant improvement the accuracy of detection algorithms has achieved result emergence open-source software innovative neural network architectures. Automated now enables extraction quantifiable cellular spatial features from microscope images cells tissues, providing insights into organization various diseases. This review aims to examine latest AI DL mining microscopy images, aid biologists who less background knowledge machine (ML), incorporate ML models focus images.

Language: Английский

Citations

2
Advances in screening hyperthermic nanomedicines in 3D tumor models DOI Creative Commons
Joana F. Soeiro, Filipa L. Sousa, Maria V. Monteiro

et al.

Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 9(3), P. 334 - 364

Published: Jan. 1, 2024

Integrating the advances of emerging hyperthermia techniques with 3D tumor models and non-invasive temperature control systems can contribute to identifying top-performing hyperthermic nanomedicines in preclinical evaluation stages.

Language: Английский

Citations

13
Open and closed microfluidics for biosensing DOI Creative Commons

Tianxin Ge,

Wenxu Hu,

Zilong Zhang

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101048 - 101048

Published: April 4, 2024

Biosensing is vital for many areas like disease diagnosis, infectious prevention, and point-of-care monitoring. Microfluidics has been evidenced to be a powerful tool biosensing via integrating biological detection processes into palm-size chip. Based on the chip structure, microfluidics two subdivision types: open closed microfluidics, whose operation methods would diverse. In this review, we summarize fundamentals, liquid control methods, applications of separately, point out bottlenecks, propose potential directions microfluidics-based biosensing.

Language: Английский

Citations

9
Introduction to Animal Cell Culture DOI
Carlos Ricardo Soccol, Vanete Thomaz‐Soccol,

Gabriela dos Santos Costa

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

9
Synthetic and natural polymer hydrogels: A review of 3D spheroids and drug delivery DOI
Paramjeet Yadav, Shiwani Singh, Sheetal Jaiswal

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 280, P. 136126 - 136126

Published: Sept. 28, 2024

Language: Английский

Citations

8
Advantages of Using 3D Spheroid Culture Systems in Toxicological and Pharmacological Assessment for Osteogenesis Research DOI Open Access
Chawon Yun, Sou Hyun Kim,

Kyung Mok Kim

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2512 - 2512

Published: Feb. 21, 2024

Bone differentiation is crucial for skeletal development and maintenance. Its dysfunction can cause various pathological conditions such as rickets, osteoporosis, osteogenesis imperfecta, or Paget's disease. Although traditional two-dimensional cell culture systems have contributed significantly to our understanding of bone biology, they fail replicate the intricate biotic environment tissue. Three-dimensional (3D) spheroid cultures gained widespread popularity addressing defects. This review highlights advantages employing 3D investigate differentiation. It their capacity mimic complex in vivo cellular interactions pivotal homeostasis. The exploration models research offers enhanced physiological relevance, improved predictive capabilities, reduced reliance on animal models, which advancement safer more effective strategies drug development. Studies highlighted transformative potential expanding biology developing targeted therapeutic interventions bone-related disorders. explores how demonstrated promise unraveling mechanisms governing homeostasis responses pharmacological agents.

Language: Английский

Citations

7
Three‐dimensional multicellular biomaterial platforms for biomedical application DOI Creative Commons

Jianxin Hao,

Qin Chen, Chengtie Wu

et al.

Interdisciplinary materials, Journal Year: 2023, Volume and Issue: 2(5), P. 714 - 734

Published: Sept. 1, 2023

Abstract The three‐dimensional (3D) multicellular platforms prepared by cells or biomaterials have been widely applied in biomedical fields for the regeneration of complex tissues, exploration cell crosstalk, and establishment tissue physiological pathological models. Compared with traditional 2D culture methods, 3D are easier to adjust components structures extracellular matrix (ECM) because synthesis ECM use biomaterials. Moreover, also can customize distribution precisely design micro macro systems. Based on these typical advantages their increasingly important position field, this review summarizes present platforms. Herein, current divided into two major types: scaffold‐free scaffold‐based specific characteristics applications different types thoroughly introduced help readers understand how models affect regulate behaviors inspire researchers select suitable according application scenarios.

Language: Английский

Citations

14
Transforming Toxicity Assessment through Microphysiology, Bioprinting, and Computational Modeling DOI Open Access

Tamer A. Addissouky

Advances in Clinical Toxicology, Journal Year: 2024, Volume and Issue: 9(1), P. 1 - 14

Published: Jan. 1, 2024

Background: Traditional toxicity testing emphasizes animal models with growing concerns regarding predictive capacity, throughput and ethics. Rapid innovation surrounding human cell platforms, bioengineered tissues, omics techniques computational tools offers more modern alternatives aligned expanding knowledge of chemical biological pathways. These disruptive approaches promise immense potential to transform next-generation safety assessment drug development pipelines. Purpose: This review provides clinical researchers an updated, comprehensive perspective across evolving areas focus in new methods analysis latest advances translational context. Main Body: We survey progress two- three-dimensional cultures recapitulating tissue/organ complexity impossible conventional assays. Complementing this, modeling integrates structure-activity relationships, physicochemical properties physiological interactions predict pharmacokinetics silico. Expanding model organisms add further dimensionality demographic relevance. High-throughput imaging technologies unravel mechanisms illuminate biomarkers undetectable by standard measures. Specialized show high addressing toxicodynamic intricacies within disease contexts like diabetes NAFLD. Evaluating traditional medicines phytochemicals likewise represents area growth well-suited for contemporary platforms. Future outlook weighs remarkable advantages reducing demands, enabling precision toxicology links medicine overhauling core risk frameworks. Conclusion: intends catalyze discourse on strategic optimization priorities roadmaps towards fully unlocking the yet still emerging public health these poising transformation sciences centered human-focused models.

Language: Английский

Citations

6