Polymer‐ and Lipid‐Based Nanostructures Serving Wound Healing Applications: A Review DOI
Fatma Nalan Cetin, Arn Mignon, Sandra Van Vlierberghe

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Management of hard‐to‐heal wounds often requires specialized care that surpasses the capabilities conventional treatments. Even most advanced commercial products lack functionality to meet needs wounds, especially those complicated by active infection, extreme bleeding, and chronic inflammation. The review explores how supramolecular nanovesicles nanoparticles—such as dendrimers, micelles, polymersomes, lipid‐based nanocarriers—can be key introducing wound healing monitoring properties address complex wounds. Their potential enable functions essential for next‐generation products—such hemostatic functions, transdermal penetration, macrophage polarization, targeted delivery, controlled release pharmaceutical ingredients (antibiotics, gaseous products, anti‐inflammatory drugs, growth factors)—is discussed via an extensive overview recent reports. These studies highlight integration systems in is crucial advancing toward a new generation addressing significant gaps current management practices. Current strategies improvements regarding personalized therapies, promising critically evaluated but underexplored polymer‐based nanovesicles, including polymersomes proteinosomes, healing.

Language: Английский

Functional Nucleic Acid-Stabilized Selenium Nanotechnology Enable Synergistic Healing of Diabetic Chronic Wounds DOI
Siwei Yang, Jing Wang, Yanni Tan

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Activity of silver-zinc nanozeolite-based antibiofilm wound dressings in an in vitro biofilm model and comparison with commercial dressings DOI Creative Commons

Sarah A. Alobaid,

Sweta Shrestha,

Morgan Tasseff

et al.

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 11, 2025

Abstract Background Infected wounds are a major health problem as infection can delay wound healing. Wound dressings play an important part in care by maintaining suitable environment that promotes Silver sulfadiazine have been used to prevent burn wounds. Presently, many commercial silver obtained FDA clearance. Results In this study, we report on novel dressing using microporous aluminosilicate zeolites, termed ABF-XenoMEM. and zinc ions encapsulated the zeolite supercages. We show silver-zinc (AM30) alone is effective at inhibiting biofilm formation. The encapsulation protects from rapidly precipitating biological fluids. exploit negatively charged surface associate positively quaternary ammonium (quat) with zeolite. combination of AM30 quat enhances antimicrobial activity. colloidal nature materials makes it possible make uniform deposits extracellular matrix membrane develop final (ABF-XenoMEM). optimum loading silver, zinc, was found be 30, 3.7, 221 µg/cm 2 . Using colony model, activity ABF-XenoMEM compared four well-studied silver-based towards mature biofilms Pseudomonas aeruginosa PAO1 (ATCC 4708) methicillin-resistant Staphylococcus aureus 33592). Cytotoxicity examined HepG2 cells MTT assay. Conclusion This study shows competitive extensively model. Nanozeolite-entrapped silver/zinc antimicrobials association potential for application biofilm-infected require animal clinical studies definitive proof. Graphical abstract

Language: Английский

Citations

0
Gentiopicroside targeting AKT1 activates HIF-1α/VEGF axis promoting diabetic ulcer wound healing DOI Creative Commons
Xinxia Wang, Mingyan Liu, Yao Wu

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Gentiopicroside (GSP) have been proven to accelerate the healing of diabetic ulcers (DU), but underlying molecular mechanisms remain unclear. This study aims explore mechanism by which GSP accelerates DU. The targets were firstly predicted using SuperPred, SwissTargetPrediction, and Pharmmapper databases; DU-related transcriptome data obtained from GEO database, including GSE147890, GSE68183, GSE199939; differential expression analysis was conducted Limma package, identified after summarization de-duplication. Then, Potential for treatment DU screened Venn analysis; core selected constructing a protein-protein interaction (PPI) network; GO KEGG enrichment analysis. Finally, target binding evaluated docking CETSA assay, in vitro experiments L929 cells validate findings. A total 538 10795 predicted; 215 potential wound healing; PPI network suggested that AKT1 may be DU; showed pathways such as HIF-1 VEGF are closely related with GSP, it also participates regulation various biological processes small molecule catabolism leukocyte migration exert its therapeutic effect on Molecular detection indicated can bind AKT1. experimental results confirmed significantly promote proliferation cells. Westen Blot via AKT1/HIF-1α/VEGF axis. HIF-1α/VEGF

Language: Английский

Citations

0
Carbon Dots’ Potential in Wound Healing: Inducing M2 Macrophage Polarization and Demonstrating Antibacterial Properties for Accelerated Recovery DOI

Rajesh Gujju,

Saikat Dewanjee,

Kamini Singh

et al.

ACS Applied Bio Materials, Journal Year: 2023, Volume and Issue: 6(11), P. 4814 - 4827

Published: Oct. 27, 2023

Bacterial infections and persistent inflammation can impede the intrinsic healing process of wounds. To combat this issue, researchers have delved into potential use carbon dots (CDs) in regulation counteract infections. These CDs were synthesized using a microwave-assisted hydrothermal demonstrated outstanding antibacterial antibiofilm properties against Gram-positive Gram-negative bacteria. Additionally, displayed biocompatibility at therapeutic concentrations ability to specifically target mitochondria. CD treatment effectively nullified lipopolysaccharide-triggered reactive oxygen species production by macrophages, while simultaneously promoting macrophage polarization toward an anti-inflammatory phenotype (M2), leading reduction acceleration wound healing. In vitro scratch assays also revealed that facilitated tissue-repairing stimulating epithelial cell migration during reepithelialization. vivo studies topically applied lipopolysaccharide (LPS)-stimulated wounds C57/BL6 mice significant improvements due enhanced fibroblast proliferation, angiogenesis, collagen deposition. Crucially, histological investigations showed no indications systemic toxicity vital organs. Collectively, application has shown immense speeding up wound-healing regulating inflammation, preventing bacterial infections, tissue repair. results suggest further clinical translation should be considered.

Language: Английский

Citations

7
Pulsed Radiofrequency Electromagnetic Fields as Modulators of Inflammation and Wound Healing in Primary Dermal Fibroblasts of Ulcers DOI Creative Commons
Erica Costantini, Lisa Aielli, Giulio Gualdi

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(4), P. 357 - 357

Published: April 5, 2024

Venous leg ulcers are one of the most common nonhealing conditions and represent an important clinical problem. The application pulsed radiofrequency electromagnetic fields (PRF-EMFs), already applied for pain, inflammation, new tissue formation, can a promising approach venous ulcer amelioration. This study aims to evaluate effect PRF-EMF exposure on inflammatory, antioxidant, cell proliferation, wound healing characteristics human primary dermal fibroblasts collected from patients. cells’ proliferative migratory abilities were evaluated by means BrdU assay scratch assay, respectively. inflammatory response was investigated through TNFα, TGFβ, COX2, IL6, IL1β gene expression analysis PGE2 production, while antioxidant activity tested measuring GSH, GSSG, tGSH, GR levels. emphasizes ability PRF-EMFs modulate IL1β, TNFα in exposed ulcers. Moreover, it confirms improvement index presented PRF-EMFs. In conclusion, strategy help repair, regulating mediators involved process.

Language: Английский

Citations

2
Application of pulsed electric field technology to skin engineering DOI Creative Commons

Caitlin Berry-Kilgour,

Lisa Wise, Jessie King

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: April 16, 2024

Tissue engineering encompasses a range of techniques that direct the growth cells into living tissue construct for regenerative medicine applications, disease models, drug discovery, and safety testing. These have been implemented to alleviate clinical burdens impaired healing skin, bone, other tissues. Construct development requires integration tissue-specific and/or an extracellular matrix-mimicking biomaterial structural support. Production such constructs is generally expensive environmentally costly, thus eco-sustainable approaches should be explored. Pulsed electric field (PEF) technology nonthermal physical processing method commonly used in food production biomedical applications. In this review, key principles PEF application skin will discussed, with emphasis on how can applied modify their behaviour, biomaterials assist isolation or sterilisation, properties. The findings indicate success reliant systematic evaluation parameters, as strength, impact different cell types. Linking tangible input parameters biological responses critical sustainable tool repair

Language: Английский

Citations

2
Porous silicon-based sensing and delivery platforms for wound management applications DOI
Wei Duan,

Jingwen Zhao,

Yue Gao

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 371, P. 530 - 554

Published: June 14, 2024

Language: Английский

Citations

2
Trends in Protein Derived Materials for Wound Care Applications DOI
Muhammad Zubair,

Saadat Hussain,

Mujeeb-ur- Rehman

et al.

Biomaterials Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Natural resource based polymers, especially those derived from proteins, have attracted significant attention for their potential utilization in advanced wound care applications. Protein materials provide superior biocompatibility, biodegradability, and other functionalities compared to conventional dressings. The effectiveness of various fabrication techniques, such as electrospinning, phase separation, self-assembly, ball milling, is examined the context developing protein-based healing. These methods produce a wide range forms, including hydrogels, scaffolds, sponges, films, bioinspired nanomaterials, each designed specific types wounds different stages This review presents comprehensive analysis recent research that investigates transformation proteins into healing Our focus on essential keratin, collagen, gelatin, silk, zein, albumin, we emphasize distinct traits roles management. Protein-based show promising biomedical engineering, offering improved capabilities reduced risks infection. It crucial explore use these clinical settings while also addressing challenges may arise commercialization future.

Language: Английский

Citations

2
Critical Analysis of Cytoplasmic Progression of Inflammatory Signaling Suggests Potential Pharmacologic Targets for Wound Healing and Fibrotic Disorders DOI Creative Commons
Michael L. Samulevich, Liam E. Carman, Brian J. Aneskievich

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2723 - 2723

Published: Nov. 28, 2024

Successful skin wound healing is dependent on an interplay between epidermal keratinocytes and dermal fibroblasts as they react to local extracellular factors (DAMPs, PAMPs, cytokines, etc.) surveyed from that environment by numerous membrane receptors (e.g., TLRs, cytokine receptors, etc.). In turn, those are the start of a cytoplasmic signaling pathway where balance key effective and, needed, cell matrix regeneration. When directed through NF-κB, these routes lead transient responses benefit initiating immune recruitment, replication, chemokine production, protein synthesis. The converse can also occur, ongoing canonical NF-κB activation leads chronic, hyper-responsive states. Here, we assess three players, TAK1, TNFAIP3, TNIP1, in regulation activation, which, because their distinctive yet inter-related functions, either promote or limit activation. Their balanced function integral successful healing, given significant control over expression inflammation-, fibrosis-, remodeling-associated genes. Intriguingly, proteins have been emphasized dysregulated central systemic sclerosis (SSc). Notably, diffuse SSc shares some tissue features similar excessive inflammatory/fibrotic response without eventual resolution. Taking cue certain instances aberrant having shared aspects, e.g., chronic inflammation fibrosis, this review looks for first time, our knowledge, at what pathologies might common regarding progression NF-κB-mediated signaling. Additionally, while TNIP1 often investigated reported individually, propose them here whose consequences very highly interconnected focus NF-κB. We thus highlight emerging promise clinical derived improved understanding signal modulators. Depending protein, its indirect direct pharmacological has reported. Current findings support further intensive studies points both basic healthy cells well with goal targeting translational multiple cutaneous situations, whether stemming acute injury response.

Language: Английский

Citations

2
Ceramic Dressings—A New Non-Pharmacological Therapeutic Option in the Management of Chronic Wounds? DOI Open Access
Andrzej Hecker, Nikolaus Watzinger, Anna-Lisa Pignet

et al.

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(5), P. 498 - 498

Published: May 8, 2024

A new ceramic dressing, free from active antimicrobial or pharmaceutical agents, uses physical binding mechanisms for its absorption capacities and bacterial-binding properties. The purpose of this study was to evaluate wound healing, bacterial-related retention, diagnostic properties dressings in patients with stagnated chronic wounds. Methods: In monocentric, intra-individually controlled, prospective study, conservatively treated refractory wounds were enrolled. One week before the start application it ensured during a screening phase that showed less than 10% reduction size. During 4-week dressing treatment size measurements, scoring, measurement exudate amount, swabs, sonication (low-intensity ultrasound) carried out. fluid removed used analysis bacterial retention compared swabs. Results: total 20 mean age 64.6 years (±26.2) 21 included study. After treatment, significant median 1178 mm2 (range 104–6300) 751.5 16–4819) better scores observed (p < 0.001). sensitivity bacteria detection 90.7% dressings, while only 76.9% conventional Conclusion: seems have positive impact on healing Bacteria-binding characteristics investigated combination debridement, absorption, detoxification properties, could contribute abilities. Based those results, be promising option without use any pharmacological agents. Moreover, can also considered microbiological purposes.

Language: Английский

Citations

1