Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: July 30, 2024
Language: Английский
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: Aug. 21, 2024
Bone defects pose significant challenges in healthcare, with over 2 million bone repair surgeries performed globally each year. As a burgeoning force the field of tissue engineering, 3D printing offers novel solutions to traditional transplantation procedures. However, current 3D-printed scaffolds still face three critical material selection, methods, cellular self-organization and co-culture, significantly impeding their clinical application. In this comprehensive review, we delve into performance criteria that ideal should possess, particular focus on core faced by technology during translation. We summarize latest advancements non-traditional materials advanced techniques, emphasizing importance integrating organ-like technologies bioprinting. This combined approach enables more precise simulation natural structure function. Our aim writing review is propose effective strategies address these promote translation for defect treatment.
Language: Английский
Citations
21
Small, Journal Year: 2024, Volume and Issue: unknown
Published: May 28, 2024
Abstract Infected bone defects are one of the most challenging problems in treatment due to high antibiotic failure rate and lack ideal grafts. In this paper, inspired by clinical cement filling treatment, α ‐c phosphate ( ‐TCP) with self‐curing properties is composited β ‐tricalcium constructed a bionic cancellous scaffolding system α/β‐tricalcium / low‐temperature 3D printing, gelatin preserved inside scaffolds as an organic phase, later loaded metal–polyphenol network structure tea polyphenol‐magnesium (TP‐Mg) nanoparticles. The mimic components mechanical strength (>100 MPa) based on ‐TCP through printing. Meanwhile, TP‐Mg exhibit significant inhibition Staphylococcus aureus S.aureus ) promote transition macrophages from M1 pro‐inflammatory M2 anti‐inflammatory phenotype. addition, composite scaffold also exhibits excellent bone‐enhancing effects synergistic effect Mg 2+ Ca . study, multifunctional ceramic ‐TCP@TP‐Mg) that integrates anti‐inflammatory, antibacterial, osteoinduction constructed, which promotes late regenerative healing while modulating early microenvironment infected defects, has promising application defects.
Language: Английский
Citations
19
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 23, 2025
Rheumatoid arthritis (RA), a form of autoimmune inflammation, is marked by enduring synovial inflammation and the subsequent impairment joint function. Despite availability conventional treatments, they are often marred significant side effects associated high costs. Plant-derived extracellular vesicles (PEVs) offer compelling alternative, owing to their abundant availability, affordability, low immunogenicity, biocompatibility, feasibility for large-scale production. These enhance intercellular communication transferring intrinsic bioactive molecules. In our research, we delve into capacity PEVs treat RA, highlighting role ginger-derived (GDEVs). By conjugating GDEVs with folic acid (FA), have developed FA-GDEVs that maintain inherent immunomodulatory properties. designed selectively target M1 macrophages in inflamed joints via folate receptors (FRs). Our vitro findings indicate promote polarization towards reparative M2 macrophage phenotype modulating PI3K-AKT pathway. Further corroboration comes from vivo studies, which demonstrate not only concentrate efficiently affected but also markedly reduce manifestations RA. Synthesizing these findings, it evident emerge as hopeful candidate RA treatment, offering benefits such safety, therapeutic efficacy.
Language: Английский
Citations
6
Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101502 - 101502
Published: Jan. 19, 2025
DNA-based hydrogels stand out for bone regeneration due to their exceptional biocompatibility and programmability. These facilitate the formation of spatial structures through bulk hydrogel fabricating, microsphere formatting, 3D printing. Furthermore, microenvironment can be finely tuned by leveraging degradation products, nanostructure, targeting, delivery capabilities inherent materials. In this review, we underscore advantages hydrogels, detailing composition, gelation techniques, structure optimization. We then delineate three critical elements in promotion using hydrogels: (i) osteogenesis driven phosphate ions, plasmids, oligodeoxynucleotides (ODNs) that enhance mineralization promote gene protein expression; (ii) vascularization facilitated tetrahedral DNA nanostructures (TDNs) aptamers, which boosts expression targeted release; (iii) immunomodulation achieved loaded factors, TDNs, bound ions stimulate macrophage polarization exhibit antibacterial properties. With these properties, used construct organoids, providing an innovative tool disease modeling therapeutic applications tissue engineering. Finally, discuss current challenges future prospects, emphasizing potential impacts regenerative medicine.
Language: Английский
Citations
3
Advanced Materials, Journal Year: 2025, Volume and Issue: unknown
Published: March 23, 2025
Abstract Bone organoids, in vitro models mimicking native bone structure and function, rely on 3D stem cell culture for self‐organization, differentiation, ECM secretion, biomineralization, ultimately forming mineralized collagen hierarchies. However, their development is often limited by the lack of suitable matrices with optimal mechanical properties sustained growth differentiation. To address this, a dynamic DNA/Gelatin methacryloyl (GelMA) hydrogel (CGDE) developed to recapitulate key biochemical features ECM, providing supportive microenvironment organoid formation. This dual‐network engineered through hydrogen bonding between DNA GelMA, combined GelMA network crosslinking, resulting appropriate strength enhanced viscoelasticity. During 21‐day culture, CGDE facilitates cellular migration promoting woven (WBO) formation via intramembranous ossification. These WBOs exhibit spatiotemporal architectures supporting mineralization tissue remodeling. In vivo studies demonstrate that CGDE‐derived self‐adaptive properties, enabling rapid osseointegration within 4 weeks. work highlights as robust scalable platform development, offering new insights into biology innovative strategies regeneration.
Language: Английский
Citations
3
Aggregate, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 9, 2025
ABSTRACT The repair and functional reconstruction of bone defects resulting from trauma, surgical resection, degenerative diseases, congenital malformations are major clinical challenges. Bone tissue engineering has significant advantages in the treatment severe defects. Vascularized scaffolds gradually attracting attention development because their excellent biomimetic properties efficient efficiency. Three‐dimensional (3D) printing technology, which can be used to fabricate structures at different scales using a wide range materials, been production vascularized scaffolds. This review discusses research progress 3D for Angiogenesis‐osteogenesis coupling regeneration process is first introduced, followed by summary technologies, inks, bioactive factors Notably, this focuses on structural design strategies Finally, application medicine, as well challenges outlooks future development, described.
Language: Английский
Citations
2
Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: March 26, 2025
An ideal guided bone regeneration (GBR) membrane for periodontitis treatment should incorporate biocompatibility, biodegradability, mechanical strength, antibacterial properties, and osteoconductivity. However, no commercially available GBR meets all these criteria simultaneously. In this study, a novel biodegradable piezoelectric double-layered is developed, with non-piezoelectric Poly-L-lactic acid (PLLA) side facing the gingiva PLLA-ZnO alveolar bone. This asymmetric membrane, distinct fiber orientations charge distribution, combines synergizes degradability, barrier function, activity osteogenic potential to enhance efficacy. The can effectively prevent fibroblast migration, inhibits bacterial infection, promotes both in vitro vivo. testing shows good rate against Porphyromonas gingivalis (P. gingivalis) Staphylococcus aureus (S. aureus) after 10 min of ultrasound stimulation. Expression levels genes Bone morphogenetic Protein 2 (BMP2), Runt-related transcription factor (RUNX2), Osteopontin (OPN) Osteocalcin (OCN) are over twice that control. mouse P. gingivalis-mediated model, our composite demonstrates effective antimicrobial effects promote 2- 4-weeks implantation, facilitated by mechanisms such as physical isolation, zinc ion release, effects, enhanced expression through activation osteogenesis-related signaling pathways, underscoring its strong applications.
Language: Английский
Citations
2
Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e36258 - e36258
Published: Aug. 1, 2024
Natural bone tissue has the certain function of self-regeneration and repair, but it is difficult to repair large damage. Recently, although autologous grafting "gold standard" for improving high cost, few donor sources. Besides, allogeneic causes greater immune reactions, which hardly meet clinical needs. The engineering (BTE) been developed promote repair. Gelatin, due its biocompatibility, receives a great deal attention in BTE research field. However, disadvantages natural gelatin are poor mechanical properties single structural property. With development BTE, often used combination with range natural, synthetic polymers, inorganic materials achieve synergistic effects complex physiological process review delves into fundamental structure unique gelatin, as well excellent necessary scaffold materials. Then this explores application modified three-dimensional (3D) scaffolds various structures including 3D fiber scaffolds, hydrogels, nanoparticles. In addition, focuses on efficacy composite consisting or polymeric materials, bioactive ceramics metallic/non-metallic defects. these gelatin-based provides new ideas design good biosafety.
Language: Английский
Citations
15
Small, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 8, 2024
Abstract The repair and reconstruction of large‐scale bone defects face enormous challenges because the failure to reconstruct osteo‐vascularization network. Herein, a near‐infrared (NIR) light‐responsive hydrogel system is reported achieve programmed tissue regeneration through synergetic effects on‐demand drug delivery mild heat stimulation. spatiotemporal (HG/MPa) composed polydopamine‐coated Ti 3 C 2 T x MXene (MP) nanosheets decorated with acidic fibroblast growth factor (aFGF, potent angiogenic drug) hydroxypropyl chitosan/gelatin (HG) developed orchestrate network boost regeneration. Upon exposure NIR light irradiation, engineered HG/MPa can initial complete release aFGF induce rapid angiogenesis provide sufficient blood supply, maximizing its biofunction in defect area. This integrated demonstrated good therapeutic efficacy promoting cell adhesion, proliferation, migration, angiogenesis, osteogenic differentiation periodic irradiation. In vivo, animal experiments further revealed that spatiotemporalized platform synergized photothermal treatment significantly accelerated critical‐sized healing by increasing density, recruiting endogenous stem cells, facilitating production osteogenesis/angiogenesis‐related factors. Overall, smart‐responsive could enhance
Language: Английский
Citations
15
Bone Research, Journal Year: 2024, Volume and Issue: 12(1)
Published: Nov. 20, 2024
Abstract The concept and development of bone/cartilage organoids are rapidly gaining momentum, providing opportunities for both fundamental translational research in bone biology. Bone/cartilage organoids, essentially miniature tissues grown vitro, enable the study complex cellular interactions, biological processes, disease pathology a representative controlled environment. This review provides comprehensive up-to-date overview field, focusing on strategies organoid construction strategies, progresses research, potential applications. We delve into significance selecting appropriate cells, matrix gels, cytokines/inducers, techniques. Moreover, we explore role advancing our understanding reconstruction, modeling, drug screening, prevention, treatment strategies. While acknowledging these discuss inherent challenges limitations field propose solutions, including use bioprinting induction, AI improved screening exploration assembloids more complex, multicellular models. believe that with continuous refinement standardization, can profoundly impact patient-specific therapeutic interventions lead way regenerative medicine.
Language: Английский
Citations
15