Nature Chemistry, Journal Year: 2024, Volume and Issue: 16(11), P. 1748 - 1750
Published: Oct. 15, 2024
Language: Английский
Nature Nanotechnology, Journal Year: 2024, Volume and Issue: unknown
Published: July 1, 2024
The clustering of death receptors (DRs) at the membrane leads to apoptosis. With goal treating tumours, multivalent molecular tools that initiate this mechanism have been developed. However, DRs are also ubiquitously expressed in healthy tissue. Here we present a stimuli-responsive robotic switch nanodevice can autonomously and selectively turn on display cytotoxic ligand patterns tumour microenvironments. We demonstrate switchable DNA origami normally hides six ligands but displays them as hexagonal pattern 10 nm diameter once under higher acidity. This effectively cluster trigger apoptosis human breast cancer cells pH 6.5 while remaining inert 7.4. When administered mice bearing xenografts, decreased growth by up 70%. data feasibility opportunities for developing switches path targeted treatment.
Language: Английский
Citations
17
Nano Letters, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 17, 2025
Watson-Crick and Hoogsteen hydrogen bonds aid the formation of highly ordered structures in genomic DNA that dynamically govern genetic modes such as gene regulation replication. Hence, measuring distinguishing these two types different contexts are essential for understanding architectures. However, due to their transient nature minimal structure differences at sub-nanometer scale, differentiating from is difficult. Relying on nanopore technology, we successfully discriminated multiple presence epigenetic modification, changes structures, proton strength environment. Our results indicate show susceptibilities physicochemical characteristics matter stabilizing bonds. This work provides insight into features nanoscale may benefit profiling complex architectures by subtle structural changes.
Language: Английский
Citations
2
Langmuir, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 6, 2025
In fabricating new colloid-based materials via bottom-up design, particle-particle interactions are engineered to encourage the formation of desired assemblies. One way do this is apply an external field, which orients magnetically polarized particles in field direction. External fields have advantage that they can be programmed change time (e.g., rotation or toggling), tunably shifting system away from equilibrium. Here, we a model for ferromagnetic colloidal rods simulates their phase behavior presence magnetic with constant strength and An annealing process slowly reduces temperature during molecular dynamics simulations estimate system's equilibrium configuration ground state when between dominate thermal forces. Numerous performed at various particle densities strengths. absence assemble into antiparallel configurations. When sufficiently strong, forced orient direction therefore form head-to-tail structures. The associated net moment results collective alignment all Furthermore, systems state, occupy more space than most assembled Phase diagrams predict properties rod-like switch nonmagnetic states by tuning not only but also density.
Language: Английский
Citations
2
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(9), P. 3687 - 3697
Published: Feb. 14, 2024
DNA nanotechnology is rapidly gaining traction in numerous applications, each bearing varying degrees of tolerance to the quality and quantity necessary for viable nanostructure function. Despite distinct objectives application, they are united their reliance on essential analytical techniques, such as purification characterization. This tutorial aims guide reader through current state chemistry, outlining important factors consider when designing, assembling, purifying, characterizing a downstream applications.
Language: Английский
Citations
13
Medical Review, Journal Year: 2024, Volume and Issue: 4(3), P. 207 - 224
Published: March 26, 2024
Abstract RNA-based therapeutics have emerged as a promising approach for the treatment of various diseases, including cancer, genetic disorders, and infectious diseases. However, delivery RNA molecules into target cells has been major challenge due to their susceptibility degradation inefficient cellular uptake. To overcome these hurdles, DNA-based nano technology offers an unprecedented opportunity potential platform therapeutics. Due its excellent characteristics such programmability biocompatibility, nanostructures, composed DNA assembled precise programmable structures, garnered significant attention ideal building materials protecting delivering payloads desired destinations. In this review, we highlight current progress in design application three nanostructures: origami, lipid-nanoparticle (LNP) related frame guided assembly (FGA), hydrogel molecules. Their biomedical applications are briefly discussed challenges future perspectives field also highlighted.
Language: Английский
Citations
5
Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(37), P. 25422 - 25425
Published: Sept. 5, 2024
Self-assembly of biomolecules provides a powerful tool for wide range applications in nanomedicine, biosensing and imaging, vaccines, computation, nanophotonics, etc. The key is to rationally design building blocks the intermolecule interactions. Along this line, structural DNA nanotechnology has rapidly developed by limiting secondary structures primarily well-established, B-form duplexes, which can be readily reliably predicted. As field evolves, more sophisticated elements must introduced. While increasing complexity, they bring challenges predicting nanostructures. In past, brutal tedious error-and-trial approach often been used solve problem. Here, we report case study applying AlphaFold 3 model facilitate nanostructure design. This protocol expected generally applicable greatly facilitates further development nanotechnology.
Language: Английский
Citations
5
Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(51)
Published: Oct. 31, 2023
Abstract Hierarchical DNA nanostructures offer programmable functions at scale, but making these structures dynamic, while keeping individual components intact, is challenging. Here we show that the A‐motif—protonated, self‐complementary poly(adenine) sequences—can propagate origami into one‐dimensional, micron‐length fibrils. When coupled to a small molecule pH regulator, visible light can activate hierarchical assembly of our dissipative This system recyclable and does not require modification. By employing modular waste‐free strategy assemble disassemble built from origami, facile accessible route developing well‐defined, large assemblies with temporal control. As general tool, envision coupling A‐motif cycles protonation will allow transient construction diverse nanostructures, finding broad applications in dynamic non‐equilibrium nanotechnology.
Language: Английский
Citations
11
Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(19), P. 13617 - 13628
Published: May 2, 2024
Here, we present a cross-linking approach to covalently functionalize and stabilize DNA origami structures in one-pot reaction. Our strategy involves adding nucleotide sequences adjacent staple strands, so that, upon assembly of the structure, extensions form short hairpin duplexes targetable by psoralen-labeled triplex-forming oligonucleotides bearing other functional groups (pso-TFOs). Subsequent irradiation with UVA light generates psoralen adducts one or both staples leading site-specific attachment pso-TFO (and attached group) ca. 80% efficiency. Bis-adduct formation between strands proximal hairpins further tethers TFO structure "superstaples" that improve structural integrity functionalized complex. We show directing regions outside core dramatically reduces sensitivity thermal denaturation disassembly T7 RNA polymerase. also underlying duplex are digested DNase I thus remain accessible read-out DNA-binding proteins. is scalable cost-effective, as it works existing structures, does not require scaffold redesign, can be achieved just psoralen-modified oligonucleotide.
Language: Английский
Citations
4
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159425 - 159425
Published: Jan. 1, 2025
Language: Английский
Citations
0
Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 28, 2025
Recently, much attention has been focused on oligonucleotide drugs that precisely control the gene expression. Among these, triplex-forming oligonucleotides (TFOs) represent common antigene strategies because they bind specifically to major groove position of genomic DNA form a triplex structure. Thus far, this promising formation technique represents successful strategy with strong application prospects for manipulation applications (e.g., cancer, Huntington's disease, inflammatory etc.), analytical detection nucleic acid, small molecules, and nanotechnology molecular machines, etc.). This review summarizes in detail full range potential described above, particularly various chemical modification have facilitated stepwise advancement TFO-based recent years improve effectiveness, specificity, applicability synergistically promote effectiveness DNA.
Language: Английский
Citations
0