Advances in the Model Structure of In Vitro Vascularized Organ-on-a-Chip DOI Creative Commons

Hongze Yin,

Yue Wang, Na Liu

et al.

Cyborg and Bionic Systems, Journal Year: 2024, Volume and Issue: 5

Published: Jan. 1, 2024

Microvasculature plays a crucial role in human physiology and is closely related to various diseases. Building vitro vascular networks essential for studying tissue behavior with repeatable morphology signaling conditions. Engineered 3D microvascular network models, developed through advanced microfluidic-based techniques, provide accurate reproducible platforms the microvasculature vitro, an component designing organ-on-chips achieve greater biological relevance. By optimizing microstructure of microfluidic devices mimic vivo microenvironment, organ-specific models healthy pathological tissues can be created. This review summarizes recent advancements strategies constructing devices. It discusses static vascularization chips’ classification, structural characteristics, techniques used build them: growing blood vessels on chips either or dynamic, grown microchannels, elastic membranes, hydrogels. Finally, paper application scenarios key technical issues existing chips. also explores potential novel organoid chip approach that combines organoids organ generate better

Language: Английский

Kidney Decellularized Extracellular Matrix Enhanced the Vascularization and Maturation of Human Kidney Organoids DOI
Jin Won Kim, Sun Ah Nam, Jawoon Yi

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(15)

Published: March 24, 2022

Kidney organoids derived from human pluripotent stem cells (hPSCs) have extensive potential for disease modelling and regenerative medicine. However, the limited vascularization immaturity of kidney been still remained to overcome. Extracellular matrix (ECM) can provide mechanical support a biochemical microenvironment cell growth differentiation. Here in vitro methods using decellularized extracellular (dECM) hydrogel culture hPSC-derived organoids, which vascular network their own endothelial cells, are reported. Single-cell transcriptomics reveal that vascularized cultured dECM more mature patterns glomerular development higher similarity than those without dECM. Differentiation α-galactosidase A (GLA)-knock-out hPSCs generated CRISPR/Cas9 into by method efficiently recapitulate Fabry nephropathy with vasculopathy. Transplantation mouse accelerates recruitment host maintains integrity organized slit diaphragm-like structures The methodology inducing maturation be applied studies development, modeling,

Language: Английский

Citations

117
Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions DOI Creative Commons

Peilin Lu,

Dongxue Ruan,

Meiqi Huang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: July 1, 2024

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.

Language: Английский

Citations

94
Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering DOI Creative Commons
Adam D. McInnes, Mike Moser, Daniel Chen

et al.

Journal of Functional Biomaterials, Journal Year: 2022, Volume and Issue: 13(4), P. 240 - 240

Published: Nov. 14, 2022

The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize practise through abilities repair, regenerate, or replace tissues organs with functional engineered constructs. To this end, combines scaffolding materials cells biologically active molecules into constructs appropriate structures properties for tissue/organ regeneration, where biomolecules are keys mimic native extracellular matrix (ECM). For this, one emerging way is decellularize ECM suitable for, directly in combination other materials, creating Over past decade, decellularized (or dECM) has greatly facilitated advance medicine, while being challenged many ways. This article reviews recent development dECM a focus on preparation along its influence cell culture, modification use as material, novel techniques trends processing We highlight success vitro, vivo, clinical applications further identify key issues challenges involved, discussion future research directions.

Language: Английский

Citations

74
3D bioprinted vascularized lung cancer organoid models with underlying disease capable of more precise drug evaluation DOI
Yoo‐mi Choi, Haram Lee,

Minjun Ann

et al.

Biofabrication, Journal Year: 2023, Volume and Issue: 15(3), P. 034104 - 034104

Published: May 26, 2023

Abstract Despite encouraging progress in the development of vitro cancer models, models that simultaneously recapitulate complexity tumor microenvironment and its diverse cellular components genetic properties remain lacking. Here, an advanced vascularized lung (LC) model is proposed, which includes patient-derived LC organoids (LCOs), fibroblasts, perfusable vessels using 3D bioprinting technology. To better biochemical composition native tissues, a porcine lung-derived decellularized extracellular matrix (LudECM) hydrogel was produced to offer physical cues cells microenvironment. In particular, idiopathic pulmonary fibrosis-derived fibroblasts were used implement fibrotic niches similar actual human fibrosis. It shown they increased cell proliferation expression drug resistance-related genes LCOs with addition, changes resistance sensitizing targeted anti-cancer drugs fibrosis significantly greater LudECM than Matrigel. Therefore, assessment responsiveness can help determine appropriate therapy for patients accompanied by Furthermore, it expected this approach could be utilized therapies or identification biomarkers

Language: Английский

Citations

44
3D Hydrogel Encapsulation Regulates Nephrogenesis in Kidney Organoids DOI
Bryan A. Nerger,

Sumit Sinha,

Nathan N. Lee

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(14)

Published: Jan. 5, 2024

Stem cell-derived kidney organoids contain nephron segments that recapitulate morphological and functional aspects of the human kidney. However, directed differentiation protocols for are largely conducted using biochemical signals to control differentiation. Here, hypothesis mechanical regulate nephrogenesis is investigated in 3D culture by encapsulating within viscoelastic alginate hydrogels with varying rates stress relaxation. Tubular significantly more convoluted differentiated when compared those suspension culture. Hydrogel viscoelasticity regulates spatial distribution differentiating organoids. Consistent these observations, a particle-based computational model predicts extent deformation hydrogel-organoid interface morphology segments. Elevated extracellular calcium levels medium, which can be impacted hydrogels, decrease glomerulus-to-tubule ratio These findings reveal hydrogel encapsulation patterning suggest microenvironment an important design variable regenerative medicine.

Language: Английский

Citations

29
Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status DOI
Soumitra Das, Jeyapriya Thimukonda Jegadeesan, Bikramjit Basu

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(4), P. 2156 - 2221

Published: March 20, 2024

Tissue engineering for injured tissue replacement and regeneration has been a subject of investigation over the last 30 years, there considerable interest in using additive manufacturing to achieve these goals. Despite such efforts, many key questions remain unanswered, particularly area biomaterial selection applications as well quantitative understanding process science. The strategic utilization biological macromolecules provides versatile approach meet diverse requirements 3D printing, printability, buildability, biocompatibility. These molecules play pivotal role both physical chemical cross-linking processes throughout biofabrication, contributing significantly overall success printing process. Among several bioprintable materials, gelatin methacryloyl (GelMA) widely utilized applications, with some degree success. In this context, review will discuss bioengineering approaches identify gelation strategies that are appropriate control rheology, buildability inks. This focus on GelMA structural (scaffold) different tissues potential carrier vehicle transport living cells their maintenance viability physiological system. Recognizing importance printability toward shape fidelity biophysical properties, major qualitative impact factors, including microrheological, viscoelastic, gelation, shear thinning properties inks, parameters, particular, reference extrusion GelMA-based Specifically, we emphasize possibilities regulate mechanical, swelling, biodegradation, cellular functionalities bio(material) by hybridization techniques, synthetic natural biopolymers, inorganic nanofillers, microcarriers. At close, possibility integration experimental data sets artificial intelligence/machine learning is emphasized predict fidelity, or inks clinically relevant tissues.

Language: Английский

Citations

26
Advancements and Prospects of Machine Learning in Medical Diagnostics: Unveiling the Future of Diagnostic Precision DOI

Sohaib Asif,

Wenhui Yi, Saif Ur-Rehman

et al.

Archives of Computational Methods in Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: June 26, 2024

Language: Английский

Citations

25
3D organ-on-a-chip: The convergence of microphysiological systems and organoids DOI Creative Commons
Leandra Santos Baptista, Constance Porrini, Gabriela S. Kronemberger

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: Nov. 21, 2022

Medicine today faces the combined challenge of an increasing number untreatable diseases and fewer drugs reaching clinic. While pharmaceutical companies have increased in early development entering phase I clinical trials, actually successfully pass III launch into market. In fact, only 1 out every 9 will launch.

Language: Английский

Citations

50
Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering DOI Creative Commons
Radman Mazloomnejad, Amirhesam Babajani, Mohammadreza Kasravi

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 16, 2023

Decellularization of tissues and organs has recently become a promising approach in tissue engineering regenerative medicine to circumvent the challenges organ donation complications transplantations. However, one main obstacle reaching this goal is acellular vasculature angiogenesis endothelialization. Achieving an intact functional vascular structure as vital pathway for supplying oxygen nutrients remains decisive challenge decellularization/re-endothelialization procedure. In order better understand overcome issue, complete appropriate knowledge endothelialization its determining variables required. methods their effectiveness, biological mechanical characteristics scaffolds, artificial bioreactors, possible applications, extracellular matrix surface modification, different types utilized cells are factors affecting consequences. This review focuses on how optimize them, well discussing recent developments process re-endothelialization.

Language: Английский

Citations

27
Vascularized Tissue Organoids DOI Creative Commons
Hannah A. Strobel, Sarah M. Moss, James B. Hoying

et al.

Bioengineering, Journal Year: 2023, Volume and Issue: 10(2), P. 124 - 124

Published: Jan. 17, 2023

Tissue organoids hold enormous potential as tools for a variety of applications, including disease modeling and drug screening. To effectively mimic the native tissue environment, it is critical to integrate microvasculature with parenchyma stroma. In addition providing means physiologically perfuse organoids, also contributes cellular dynamics model via cells perivascular niche, thereby further modulating function. this review, we discuss current developing strategies vascularizing consider tissue-specific vascularization approaches, importance perfusion, provide perspectives on state field.

Language: Английский

Citations

25