Application and prospect of microfluidic devices for rapid assay of cell activities in the tumor microenvironment DOI

Linjing Zhu,

Xueling Cui, Lingling Jiang

et al.

Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(3)

Published: May 1, 2024

The global impact of cancer on human health has raised significant concern. In this context, the tumor microenvironment (TME) plays a pivotal role in tumorigenesis and malignant progression. order to enhance accuracy efficacy therapeutic outcomes, there is an imminent requirement for vitro models that can accurately replicate intricate characteristics constituents TME. Microfluidic devices exhibit notable advantages investigating progression treatment tumors have potential become novel methodology evaluating immune cell activities TME assist clinicians assessing prognosis patients. addition, it shows great compared traditional experiments. Therefore, review first outlines applications microfluidic chips facilitating culture, constructing activities. Second, roles analysis circulating cells, prognosis, drug screening also been mentioned. Moreover, forward-looking perspective discussed, anticipating widespread clinical adoption future.

Language: Английский

Harmonized single-cell landscape, intercellular crosstalk and tumor architecture of glioblastoma DOI Creative Commons
Cristian Ruiz-Moreno, Sergio Marco Salas, Erik Samuelsson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Aug. 27, 2022

SUMMARY Glioblastoma, isocitrate dehydrogenase (IDH)-wildtype (hereafter, GB), is an aggressive brain malignancy associated with a dismal prognosis and poor quality of life. Single-cell RNA sequencing has helped to grasp the complexity cell states dynamic changes in GB. Large-scale data integration can help uncover unexplored tumor pathobiology. Here, we resolved composition milieu created cellular map GB (‘GBmap’), curated resource that harmonizes 26 datasets gathering 240 patients spanning over 1.1 million cells. We showcase applications our for reference mapping, transfer learning, biological discoveries. Our results sources pro-angiogenic signaling multifaceted role mesenchymal-like cancer Reconstructing architecture using spatially transcriptomics unveiled high level well-structured neoplastic niches. The GBmap represents framework allows streamlined interpretation new provides platform exploratory analysis, hypothesis generation testing.

Language: Английский

Citations

84
3D Biomimetic Models to Reconstitute Tumor Microenvironment In Vitro: Spheroids, Organoids, and Tumor‐on‐a‐Chip DOI Creative Commons
Wenxiu Li, Zhihang Zhou, Xiaoyu Zhou

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(18)

Published: March 14, 2023

Abstract Decades of efforts in engineering vitro cancer models have advanced drug discovery and the insight into biology. However, establishment preclinical that enable fully recapitulating tumor microenvironment remains challenging owing to its intrinsic complexity. Recent progress techniques has allowed development a new generation can recreate complex vivo microenvironments accurately predict responses, including spheroids, organoids, tumor‐on‐a‐chip. These biomimetic 3D are particular interest as they pave way for better understanding biology accelerating anticancer therapeutics with reducing animal use. Here, recent advances developing these platforms modeling screening, focusing on incorporating hydrogels reviewed reconstitute physiologically relevant microenvironments. The combination spheroids/organoids microfluidic technologies is also highlighted mimic tumors discuss challenges future directions clinical translation such screening personalized medicine.

Language: Английский

Citations

52
Investigating the Interactions of Glioma Stem Cells in the Perivascular Niche at Single‐Cell Resolution using a Microfluidic Tumor Microenvironment Model DOI
Emmanuela Adjei‐Sowah, Samantha O’Connor, Jaimeson Veldhuizen

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(21)

Published: May 26, 2022

The perivascular niche (PVN) is a glioblastoma tumor microenvironment (TME) that serves as safe haven for glioma stem cells (GSCs), and acts reservoir inevitably leads to recurrence. Understanding cellular interactions in the PVN drive GSC treatment resistance stemness crucial develop lasting therapies glioblastoma. limitations of vivo models vitro assays have led critical knowledge gaps regarding influence various cell types on GSCs behavior. This study developed an organotypic triculture microfluidic model means recapitulate its impact GSCs. platform, comprised endothelial (ECs), astrocytes, GSCs, used investigate invasion, proliferation stemness. Both ECs astrocytes significantly increased invasiveness futher identified 15 ligand-receptor pairs using single-cell RNAseq with putative chemotactic mechanisms where receptor up-regulated diffusible ligand expressed either or ECs. Notably, pair SAA1-FPR1 demonstrated be involved invasion toward PVN. novel platform presented herein can therapeutic development discovery molecular driving biology.

Language: Английский

Citations

29
Preclinical Models and Technologies in Glioblastoma Research: Evolution, Current State, and Future Avenues DOI Open Access
Hasan Slika, Ziya Karimov,

Paolo Alimonti

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16316 - 16316

Published: Nov. 14, 2023

Glioblastoma is the most common malignant primary central nervous system tumor and one of debilitating cancers. The prognosis patients with glioblastoma remains poor, management this tumor, both in its recurrent forms, suboptimal. Despite tremendous efforts that are being put forward by research community to discover novel efficacious therapeutic agents modalities, no major paradigm shifts have been established field last decade. However, does not mirror abundance relevant findings discoveries made preclinical research. Hence, developing utilizing appropriate models faithfully recapitulate characteristics behavior human utmost importance. Herein, we offer a holistic picture evolution glioblastoma. We further elaborate on commonly used vitro vivo models, delving into their development, favorable characteristics, shortcomings, areas potential improvement, which aids researchers designing future experiments suitable models. Additionally, review explores progress fields humanized immunotolerant mouse genetically engineered animal 3D microfluidics highlights promising avenues for

Language: Английский

Citations

20
Vascularized organoid-on-a-chip: design, imaging, and analysis DOI
Tingting Yu,

Qihang Yang,

Bo Peng

et al.

Angiogenesis, Journal Year: 2024, Volume and Issue: 27(2), P. 147 - 172

Published: Feb. 26, 2024

Language: Английский

Citations

8
Engineered Tumor–Immune Microenvironment On A Chip to Study T Cell–Macrophage Interaction in Breast Cancer Progression DOI
Twinkle Jina Minette Manoharan, Kalpana Ravi, Abhirami P. Suresh

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(14)

Published: Feb. 15, 2024

Evolving knowledge about the tumor-immune microenvironment (TIME) is driving innovation in designing novel therapies against hard-to-treat breast cancer. Targeting immune components of TIME has emerged as a promising approach for cancer therapy. While recent immunotherapies aim at restoring antitumor immunity, counteracting tumor escape remains challenging. Hence there pressing need to better understand complex crosstalk within TIME. Considering this imperative, study aims investigating between two abundant cell populations TIME-macrophages and T cells, progression using an organotypic 3D vitro tumor-on-a-chip (TOC) model. The TOC features distinct yet interconnected stromal entities. This triculture platform mimics TIME, embedding suitable matrix. Analysis invasion, morphometric measurements, flow cytometry results underscores substantial contribution macrophages progression, while presence cells associated with deceleration migratory behavior both macrophages. Furthermore, cytokine analyses reveal significant upregulation leptin RANTES (regulated on activation, normal Cell expressed secreted) triculture. Overall, highlights complexity critical role progression.

Language: Английский

Citations

7
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier DOI

James G. Shamul,

Zhiyuan Wang,

Hyeyeon Gong

et al.

Nature Biomedical Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 20, 2024

Language: Английский

Citations

7
Bridging the gap between tumor-on-chip and clinics: a systematic review of 15 years of studies DOI Creative Commons
Charlotte Bouquerel,

Anastasiia Dubrova,

Isabella Hofer

et al.

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(18), P. 3906 - 3935

Published: Jan. 1, 2023

Over the past 15 years, field of oncology research has witnessed significant progress in development new cell culture models, such as tumor-on-chip (ToC) systems. In this comprehensive overview, we present a multidisciplinary perspective by bringing together physicists, biologists, clinicians, and experts from pharmaceutical companies to highlight current state ToC research, its unique features, challenges it faces. To offer readers clear quantitative understanding field, conducted an extensive systematic analysis more than 300 publications related 2005 2022. key advantages over other vitro models enabling precise control various parameters. These parameters include properties extracellular matrix, mechanical forces exerted on cells, physico-chemical environment, composition, architecture tumor microenvironment. Such fine allows closely replicate complex microenvironment interactions within tumors, facilitating study cancer progression therapeutic responses highly representative manner. Importantly, incorporating patient-derived cells or xenografts, have demonstrated promising results terms clinical validation. We also examined potential for industries which adoption is expected occur gradually. Looking ahead, given high failure rate trials increasing emphasis 3Rs principles (replacement, reduction, refinement animal experimentation), hold immense research. next decade, data generated could potentially be employed discovering targets, contributing regulatory purposes, refining preclinical drug testing reducing reliance models.

Language: Английский

Citations

15
Engineered 3D ex vivo models to recapitulate the complex stromal and immune interactions within the tumor microenvironment DOI Creative Commons
Kalpana Ravi, Twinkle Jina Minette Manoharan,

Kuei-Chun Wang

et al.

Biomaterials, Journal Year: 2023, Volume and Issue: 305, P. 122428 - 122428

Published: Dec. 19, 2023

Language: Английский

Citations

13
Tumor‐On‐A‐Chip Model Incorporating Human‐Based Hydrogels for Easy Assessment of Metastatic Tumor Inter‐Heterogeneity DOI Creative Commons
Cátia F. Monteiro, Inês A. Deus, Inês Silva

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(30)

Published: April 5, 2024

Abstract The coordinated migration of invasive tumor cells is a complex and dynamic mechanism driven by diverse cellular molecular events. Unfortunately, the inherent heterogeneity within tumors raises multiple challenges in deciphering key biomarkers novel therapeutic approaches to prevent metastasis. Here, microengineered tumor‐on‐a‐chip system incorporating human platelet lysate hydrogels proposed recreate early metastatic process invasion drug response. By co‐culturing bone marrow mesenchymal stem with two cell lines distinct capability, developed model can emulate 3D microarchitecture inter‐heterogeneity regarding its intrinsic ability. recreated microenvironment supports stromal movement, evidencing synergistic tumor‐stromal cell‐extracellular matrix interactions an invading tumor. Through gene protein expression analysis exometabolomic profiling, this platform provides evidence for role environment as regulator Additionally, effect doxorubicin treatment on invasiveness biomarker profile highlights suitability established models therapy assessment. Overall, study presents useful pursue mechanistic studies events fully human‐derived microenvironment, while contributing fundamental insights into biomolecular profiling.

Language: Английский

Citations

4