3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration

Advanced Science, Journal Year: 2023, Volume and Issue: 10(12)

Published: Feb. 17, 2023

Nerve guidance conduits (NGCs) have become a promising alternative for peripheral nerve regeneration; however, the outcome of regeneration and functional recovery is greatly affected by physical, chemical, electrical properties NGCs. In this study, conductive multiscale filled NGC (MF-NGC) consisting electrospun poly(lactide-co-caprolactone) (PCL)/collagen nanofibers as sheath, reduced graphene oxide /PCL microfibers backbone, PCL internal structure developed. The printed MF-NGCs presented good permeability, mechanical stability, conductivity, which further promoted elongation growth Schwann cells neurite outgrowth PC12 neuronal cells. Animal studies using rat sciatic injury model reveal that promote neovascularization M2 transition through rapid recruitment vascular macrophages. Histological assessments regenerated nerves confirm significantly enhance regeneration, indicated improved axon myelination, muscle weight increase, function index. This study demonstrates feasibility 3D-printed with hierarchically oriented fibers can regeneration.

Language: Английский

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Sea Cucumber-Inspired Microneedle Nerve Guidance Conduit for Synergistically Inhibiting Muscle Atrophy and Promoting Nerve Regeneration

ACS Nano, Journal Year: 2024, Volume and Issue: 18(22), P. 14427 - 14440

Published: May 22, 2024

Muscle atrophy resulting from peripheral nerve injury (PNI) poses a threat to patient's mobility and sensitivity. However, an effective method inhibit muscle following PNI remains elusive. Drawing inspiration the sea cucumber, we have integrated microneedles (MNs) microchannel technology into guidance conduits (NGCs) develop bionic microneedle NGCs (MNGCs) that emulate structure piezoelectric function of cucumbers. Morphologically, MNGCs feature outer surface with outward-pointing needle tips capable applying electrical stimulation denervated muscles. Simultaneously, interior contains microchannels designed guide migration Schwann cells (SCs). Physiologically, incorporation conductive reduced graphene oxide zinc nanoparticles polycaprolactone scaffold enhances conductivity properties, facilitating SCs' migration, myelin regeneration, axon growth, restoration neuromuscular function. These combined effects ultimately lead inhibition Consequently, concept synergistic effect inhibiting promoting regeneration has capacity transform traditional approach repair find broad applications in repair.

Language: Английский

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Advancing Peripheral Nerve Regeneration: 3D Bioprinting of GelMA-Based Cell-Laden Electroactive Bioinks for Nerve Conduits

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(3), P. 1620 - 1645

Published: Feb. 12, 2024

Peripheral nerve injuries often result in substantial impairment of the neurostimulatory organs. While autograft is still largely used as "gold standard" clinical treatment option, guidance conduits (NGCs) are currently considered a promising approach for promoting peripheral regeneration. several attempts have been made to construct NGCs using various biomaterial combinations, comprehensive exploration process science associated with three-dimensional (3D) extrusion printing clinically relevant sizes (length: 20 mm; diameter: 2–8 mm), while focusing on tunable buildability electroactive inks, remains unexplored. In addressing this gap, we present here results viscoelastic properties range multifunctional gelatin methacrylate (GelMA)/poly(ethylene glycol) diacrylate (PEGDA)/carbon nanofiber (CNF)/gellan gum (GG) hydrogel bioink formulations and printability assessment experiments quantitative models. Our clearly established positive impact gellan enhancement rheological properties. Interestingly, strategic incorporation PEGDA secondary cross-linker led remarkable strength modulus by 3 8-fold, respectively. Moreover, conductive CNF addition resulted 4-fold improvement measured electrical conductivity. The use four-component ink allowed us obtain high neural cell viability 3D bioprinted constructs. conventionally cast scaffolds can support differentiation neuro-2a cells, most important has excellent cells encapsulated structures. Taken together, our findings demonstrate potential bioprinting multimodal biophysical cues developing functional yet critical-sized tissue

Language: Английский

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3D Bioprinting Approaches for Enhancing Stem Cell-Based Neural Tissue Regeneration

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Filamented Hydrogels as Tunable Conduits for Guiding Neurite Outgrowth

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101471 - 101471

Published: Jan. 11, 2025

Anisotropic scaffolds with unidirectionally aligned fibers present an optimal solution for nerve tissue engineering and graft repair. This study investigates the application of filamented light (FLight) biofabrication to create hydrogel matrices featuring highly microfilaments, facilitating neurite guidance outgrowth from encapsulated chicken dorsal root ganglion (DRG) cells. FLight employs optical modulation instability (OMI) rapidly safely (<5 s) fabricate constructs precise microfilament alignment. The tunability was demonstrated by adjusting four key parameters: stiffness, porosity, growth factor release, incorporation biological cues. Matrix stiffness fine-tuned varying projection dose, yielding ranging 0.6 5.7 kPa. Optimal occurred at a kPa, achieving 2.5 mm over 4 days. porosity modified using diffraction gratings in setup. While significant differences alignment were observed between bulk gels, further increases 40 % 70 enhanced cell migration axon bundling without significantly affecting maximal outgrowth. protein microcrystals containing (NGF) into photoresin enabled sustained need additional NGF media. Finally, laminin added resin enhance bioactivity biomaterial, resulting increase maximum 3.5 after days culture softer matrices. Overall, varied matrix properties achieved through outgrowth, highlighting importance adaptable scaffold characteristics guiding development. demonstrates potential as versatile platform creating ideal clinical applications repair engineering.

Language: Английский

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Developing conductive hydrogels for biomedical applications

Smart Medicine, Journal Year: 2023, Volume and Issue: 3(1)

Published: Sept. 15, 2023

Conductive hydrogels have attracted copious attention owing to their grateful performances, such as similarity biological tissues, compliance, conductivity and biocompatibility. A diversity of conductive been developed showed versatile potentials in biomedical applications. In this review, we highlight the recent advances hydrogels, involving various types functionalities well applications fields. Furthermore, current challenges reasonable outlook are also given. It is expected that review will provide potential guidance for advancement next-generation hydrogels.

Language: Английский

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3D nanofiber scaffolds from 2D electrospun membranes boost cell penetration and positive host response for regenerative medicine

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 8, 2024

Abstract The ideal tissue engineering scaffold should facilitate rapid cell infiltration and provide an optimal immune microenvironment during interactions with the host. Electrospinning can produce two-dimensional (2D) membranes mimicking extracellular matrix. However, their dense structure hinders penetration, thin form restricts utility. In this study, latticed hydrogels were three-dimensional (3D) printed onto electrospun membranes. This technique allowed for layer-by-layer assembly of into 3D scaffolds, which maintained resilience impressively under both dry wet conditions. We assessed cellular host responses these nanofiber scaffolds by comparing random mesh-like three different mesh sizes (250, 500, 750 μm). It was found that a size 500 μm superior M2 macrophage phenotype polarization, vascularization, matrix deposition. Furthermore, it confirmed subsequent experiments such as RNA sequencing topology may promote polarization to affecting PI3K/AKT pathway. conclusion, our work offers novel method transforming 2D scaffolds. boasts flexibility, allowing use varied in terms composition. has vast potential repair regeneration.

Language: Английский

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Permeable Bioelectronics toward Biointegrated Systems

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(10), P. 6543 - 6591

Published: May 10, 2024

Bioelectronics integrates electronics with biological organs, sustaining the natural functions of organs. Organs dynamically interact external environment, managing internal equilibrium and responding to stimuli. These interactions are crucial for maintaining homeostasis. Additionally, organs possess a soft stretchable nature; encountering objects differing properties can disrupt their function. Therefore, when electronic devices come into contact objects, permeability these devices, enabling substance exchanges mechanical compliance inherent functionality This review discusses recent advancements in permeable bioelectronics, emphasizing materials, structures, wide range applications. The also addresses current challenges potential solutions, providing insights integration

Language: Английский

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4D‐Printed MXene‐Based Artificial Nerve Guidance Conduit for Enhanced Regeneration of Peripheral Nerve Injuries

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: May 28, 2024

Abstract Repairing larger defects (>5 mm) in peripheral nerve injuries (PNIs) remains a significant challenge when using traditional artificial guidance conduits (NGCs). A novel approach that combines 4D printing technology with poly(L‐lactide‐co‐trimethylene carbonate) (PLATMC) and Ti 3 C 2 T x MXene nanosheets is proposed, thereby imparting shape memory properties to the NGCs. Upon body temperature activation, printed sheet‐like structure can quickly self‐roll into conduit‐like structure, enabling optimal wrapping around stumps. This design enhances fixation simplifies surgical procedures. Moreover, integration of microchannel expertly crafted through printing, along incorporation nanosheets, introduces electrical conductivity. feature facilitates guided directional migration cells, rapidly accelerating healing PNI. By leveraging these advanced technologies, developed NGCs demonstrate remarkable potential promoting regeneration, leading substantial improvements muscle morphology restored sciatic function, comparable outcomes achieved autogenous transplantation.

Language: Английский

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The role of graphene quantum dots in cutting‐edge medical therapies

Polymers for Advanced Technologies, Journal Year: 2024, Volume and Issue: 35(9)

Published: Sept. 1, 2024

Abstract Graphene quantum dots (GQDs), owing to their unique optical, electrical, and chemical properties, have emerged as promising nanomaterials for various biomedical applications. This review provides a comprehensive overview of the latest advancements in utilization GQDs tissue engineering, wound healing, drug delivery systems, other therapies. The inherent properties GQDs, including high biocompatibility, tunable photoluminescence, significant surface area, make them ideal candidates enhancing medical treatments diagnostics. In improve mechanical biological performance scaffolds, promoting cell proliferation differentiation. For enhance antimicrobial activity facilitate faster regeneration. Their potential DDS is highlighted by ability deliver therapeutic agents efficiently, ensuring targeted controlled release. Additionally, play crucial role therapies, particularly cancer treatment, efficacy reducing side effects. While offer diagnostics, challenges such understanding long‐term cytotoxicity at higher concentrations, need standardized synthesis methods remain critical areas further research. also discusses future directions opportunities emphasizing transformative advancing modern healthcare solutions. insights presented here contribute expanding field GQD research, highlighting significantly patient outcomes drive innovations.

Language: Английский

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