Macro/Microgel‐Encapsulated, Biofilm‐Armored Living Probiotic Platform for Regenerating Bacteria‐Infected Diabetic Wounds DOI Open Access

Huilong Xin,

Zhe Cai, Jiahui Hao

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract Infectious diabetic wounds pose an arduous threat to contemporary healthcare. The combination of refractory biofilms, persistent inflammation, and retarded angiogenesis can procure non‐unions life‐threatening complications, calling for advanced therapeutics potent orchestrate anti‐infective effectiveness, benign biocompatibility, pro‐reparative immunomodulation, angiogenic regeneration. Herein, embracing the emergent “living bacterial therapy” paradigm, a designer probiotic‐in‐hydrogel wound dressing platform is demonstrated. constructed employing “macrogel/microgel/biofilm” hierarchical encapsulation strategy, with Lactobacillus casei as model probiotic. Alginate gels, in both macro micro forms, along self‐produced probiotic served encapsulating agents. Specifically, live probiotics are enclosed within alginate microspheres, embedded into bulk matrix, cultivated facilitate biofilm self‐encasing. This multiscale confinement protected averted their inadvertent escape, while enabling sustained secretion, proper reservation, localized delivery therapeutically active metabolites, such lactic acid. resulting biosystem, validated vitro/ovo/vivo, elicited well‐balanced antibacterial activities biological compatibility, alongside prominent pro‐healing, vasculogenic anti‐inflammatory potencies, thus accelerating regeneration infected full‐thickness excisional mice. Such multiple encapsulation‐engineered “all‐in‐one” tactic may shed new light on safe efficient adoption bacteria treating chronic infectious diseases.

Language: Английский

Recent advances of hydrogels as smart dressings for diabetic wounds DOI
Xu Wang, Yuhan Yang, Weifeng Zhao

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(5), P. 1126 - 1148

Published: Jan. 1, 2024

This review highlights the latest progress of smart hydrogel dressings for bandaging, monitoring and therapy diabetic wounds.

Language: Английский

Citations

19
A Photo‐induced Cross‐Linking Enhanced A and B Combined Multi‐Functional Spray Hydrogel Instantly Protects and Promotes of Irregular Dynamic Wound Healing DOI

Guofeng Zhong,

Pengkun Lei,

Peng Guo

et al.

Small, Journal Year: 2024, Volume and Issue: 20(23)

Published: March 10, 2024

Abstract Wounds in harsh environments can face long‐term inflammation and persistent infection, which slow healing. Wound spray is a product that be rapidly applied to large irregularly dynamic wounds, quickly form protective film situ inhibit external environmental infection. In this study, biodegradable A B combined multi‐functional hydrogel developed with methacrylate‐modified chitosan (CSMA 1st ) ferulic acid (FA) as type raw materials oxidized Bletilla striata polysaccharide (OBSP) materials. The precursor CSMA ‐FA/OBSP (CSOB‐FA formed by the self‐cross‐linking of Schiff base bonds, CSMA‐FA/OBSP (CSOB‐FA) after UV–vis light, so fits wound. Rapid spraying curing provide sufficient flexibility rapidity for wounds has good injectability, adhesive, mechanical strength. rats miniature pigs, shrink promote healing infected enrichment fibrocyte populations. Therefore, multifunctional protect irregular prevent wound infection secondary injury, used safe effective treatment, prospect development.

Language: Английский

Citations

19
Self-healing cellulose-based hydrogels: From molecular design to multifarious applications DOI
Liang Yang, Hong Wang,

Yanning Yang

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 347, P. 122738 - 122738

Published: Sept. 13, 2024

Language: Английский

Citations

19
M2 macrophage-polarized anti-inflammatory microneedle patch for accelerating biofilm-infected diabetic wound healing via modulating the insulin pathway DOI Creative Commons
Yushan Yang, Limin Fan,

Jingsi Jiang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 14, 2024

Macrophages play a pivotal role in the healing of diabetic ulcers. The sustained elevation glucose levels damages insulin signaling pathway macrophages, leading to dysfunctional macrophages that struggle transition from pro-inflammatory (M1) reparative (M2) states. Therefore, modulating macrophage inflammatory responses via holds promise for ulcer treatment. Additionally, presence biofilm impedes drug penetration, and resulting immunosuppressive microenvironment exacerbates persistent infiltration M1 macrophages. we designed an array dissolvable microneedle (denoted as NPF@MN) loaded with self-assembled nanoparticles could deliver NPF nanoparticles, acid-sensitive NPF-releasing Protocatechualdehyde (PA) hypoglycemic insulin-like effects, regulating polarization anti-inflammatory M2 phenotype. this study extensively examined mechanism by which NPF@MN accelerates ulcers through activation pathway. Through RNA-seq GSEA analysis, identified reduction expression pathway-related factors such IR, IRS-1, IRS-2, SHC. Our work presents innovative therapeutic approach targeting underscores its translational potential clinical management.

Language: Английский

Citations

18
Macro/Microgel‐Encapsulated, Biofilm‐Armored Living Probiotic Platform for Regenerating Bacteria‐Infected Diabetic Wounds DOI Open Access

Huilong Xin,

Zhe Cai, Jiahui Hao

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract Infectious diabetic wounds pose an arduous threat to contemporary healthcare. The combination of refractory biofilms, persistent inflammation, and retarded angiogenesis can procure non‐unions life‐threatening complications, calling for advanced therapeutics potent orchestrate anti‐infective effectiveness, benign biocompatibility, pro‐reparative immunomodulation, angiogenic regeneration. Herein, embracing the emergent “living bacterial therapy” paradigm, a designer probiotic‐in‐hydrogel wound dressing platform is demonstrated. constructed employing “macrogel/microgel/biofilm” hierarchical encapsulation strategy, with Lactobacillus casei as model probiotic. Alginate gels, in both macro micro forms, along self‐produced probiotic served encapsulating agents. Specifically, live probiotics are enclosed within alginate microspheres, embedded into bulk matrix, cultivated facilitate biofilm self‐encasing. This multiscale confinement protected averted their inadvertent escape, while enabling sustained secretion, proper reservation, localized delivery therapeutically active metabolites, such lactic acid. resulting biosystem, validated vitro/ovo/vivo, elicited well‐balanced antibacterial activities biological compatibility, alongside prominent pro‐healing, vasculogenic anti‐inflammatory potencies, thus accelerating regeneration infected full‐thickness excisional mice. Such multiple encapsulation‐engineered “all‐in‐one” tactic may shed new light on safe efficient adoption bacteria treating chronic infectious diseases.

Language: Английский

Citations

3