Nanoarchitectonics beyond Self‐Assembly: Challenges to Create Bio‐Like Hierarchic Organization

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 59(36), P. 15424 - 15446

Published: March 14, 2020

Abstract Incorporation of non‐equilibrium actions in the sequence self‐assembly processes would be an effective means to establish bio‐like high functionality hierarchical assemblies. As a novel methodology beyond self‐assembly, nanoarchitectonics, which has as its aim fabrication functional materials systems from nanoscopic units through methodological fusion nanotechnology with other scientific disciplines including organic synthesis, supramolecular chemistry, microfabrication, and bio‐process, been applied this strategy. The application factors conventional is discussed on basis examples directed assembly, Langmuir–Blodgett layer‐by‐layer assembly. In particular, structures using bio‐active components such proteins or by combination bio‐components two‐dimensional nanomaterials, are described. Methodologies described review article highlight possible approaches nanoarchitectonics concept for creation higher functionalities structural organization.

Language: Английский

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Stepping Out of the Blue: From Visible to Near‐IR Triggered Photoswitches

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(31)

Published: May 7, 2022

Abstract Light offers unique opportunities for controlling the activity of materials and biosystems with high spatiotemporal resolution. Molecular photoswitches are chromophores that undergo reversible isomerization between different states upon irradiation light, allowing a convenient means to control their influence over system interest. However, significant limitation classical is requirement initiate switching in one or both directions using deleterious UV light poor tissue penetration. Red‐shifted hence demand have attracted keen recent research In this Review, we highlight progress towards development visible‐ NIR‐activated characterized by distinct photochromic reaction mechanisms. We hope inspire further endeavors field, full potential these tools biotechnology chemistry applications be realized.

Language: Английский

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General Principles for the Design of Visible‐Light‐Responsive Photoswitches: Tetra‐ortho‐Chloro‐Azobenzenes

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 59(48), P. 21663 - 21670

Published: Aug. 28, 2020

Molecular photoswitches enable reversible external control of biological systems, nanomachines, and smart materials. Their development is driven by the need for low energy (green-red-NIR) light switching, to allow non-invasive operation with deep tissue penetration. The lack clear design principles adaptation optimization such systems limits further applications. Here we provide a rulebook tetra-ortho-chloroazobenzenes, an emerging class visible-light-responsive photochromes, elucidating role that substituents play in defining their key characteristics: absorption spectra, band overlap, photoswitching efficiencies, half-lives unstable cis isomers. This achieved through joint photochemical theoretical analyses representative library molecules featuring varying electronic nature. A set guidelines presented enables tuning properties desired application informed photochrome engineering.

Language: Английский

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Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light‐Activated Guide RNA

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 59(23), P. 8998 - 9003

Published: March 11, 2020

We developed a new method for the conditional regulation of CRISPR/Cas9 activity in mammalian cells and zebrafish embryos using photochemically activated, caged guide RNAs (gRNAs). Caged gRNAs are generated by substituting four nucleobases evenly distributed throughout 5'-protospacer region with during synthesis. Caging confers complete suppression gRNA:dsDNA-target hybridization rapid restoration function upon optical activation. This tool offers simplicity programmability design, high spatiotemporal specificity embryos, excellent off-to-on switching, stability preserving ability to form Cas9:gRNA ribonucleoprotein complexes. novel tools control gene editing, thereby enabling investigation spatiotemporally complex physiological events obtaining better understanding dynamic regulation.

Language: Английский

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A Platinum(II) Complex of Heptamethine Cyanine for Photoenhanced Cytotoxicity and Cellular Imaging in Near‐IR Light

Angewandte Chemie International Edition, Journal Year: 2018, Volume and Issue: 57(32), P. 10263 - 10267

Published: June 25, 2018

Controlled generation of cytotoxic agents with near-IR light is a current focus photoactivated cancer therapy, including that involving platinum species. A heptamethine cyanine scaffolded Pt

Language: Английский

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Near‐Infrared Light‐Initiated Hybridization Chain Reaction for Spatially and Temporally Resolved Signal Amplification

Angewandte Chemie, Journal Year: 2019, Volume and Issue: 131(42), P. 15019 - 15023

Published: Aug. 2, 2019

Abstract Precise control over signal amplification provides unparalleled opportunities for diverse applications. However, spatiotemporally controlled has not been realized because of the lack a design methodology. The aim this study was thus to develop conceptual approach remote at chosen time and site in living cells. This system constructed by re‐engineering functional units hybridization chain reaction (HCR) combination with upconversion photochemistry, resulting an activatable HCR high spatial temporal precision near‐infrared (NIR) light. As proof concept, we demonstrate spatially temporally resolved amplified imaging messenger RNA (mRNA) ultrahigh sensitivity vitro vivo. Furthermore, using targeting subcellular sites have developed new technique NIR‐initiated mRNA exclusively within specific organelle.

Language: Английский

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Visible Light Photoredox Catalysis Using Ruthenium Complexes in Chemical Biology

Chemistry - A European Journal, Journal Year: 2019, Volume and Issue: 25(27), P. 6661 - 6672

Published: Jan. 28, 2019

Abstract The development of bioorthogonal reactions have had a transformative impact in chemical biology and the quest to expand this toolbox continues. Herein we review recent applications ruthenium‐catalyzed photoredox used biology.

Language: Английский

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Spatiotemporally Selective Molecular Imaging via Upconversion Luminescence‐Controlled, DNA‐Based Biosensor Technology

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(32)

Published: June 6, 2022

DNA-based biosensor technologies have shown great potential in chemical and biological detection. These biosensors been actively developed as probes for molecular imaging live cells animals, allowing situ detection of analytes complex systems, elucidation the roles key molecules processes, development non-invasive diagnosis image-guided surgery. Despite progress made, improving spatial-temporal precision remains a challenge this field. In Minireview, we describe concepts behind spatiotemporally selective via combination engineered, light-activatable upconversion nanotechnology. We then highlight application approach controlled various targets specific intracellular organelles, signal amplification, well regulation targeting activity to receptor proteins. finally discuss challenges perspectives possible future developments emerging

Language: Английский

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In the Search for Photocages Cleavable with Visible Light: An Overview of Recent Advances and Chemical Strategies

ChemPhotoChem, Journal Year: 2020, Volume and Issue: 5(4), P. 296 - 314

Published: Dec. 4, 2020

Abstract Photopharmacological tools enable the precise spatiotemporal control of small molecule drugs. Amongst them, caged compounds incorporate a photolabile moiety which is released under illumination, thus liberating active molecule. Caging groups have long been known and many chemical scaffolds already used in different applications. However, most initial examples are cleaved with UV light, suffers from low tissue permeability cell damage. Recently, caging that visible light reported, expand their utility. In this review, we outline strategies to increase absorption wavelengths; compare photophysical properties, discuss synthetic accessibility, exemplify some biological

Language: Английский

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Light‐Induced Dimerization Approaches to Control Cellular Processes

Chemistry - A European Journal, Journal Year: 2019, Volume and Issue: 25(54), P. 12452 - 12463

Published: July 15, 2019

Abstract Light‐inducible approaches provide a means to control biological systems with spatial and temporal resolution that is unmatched by traditional genetic perturbations. Recent developments of optogenetic chemo‐optogenetic for induced proximity in cells facilitate rapid reversible manipulation highly dynamic cellular processes have become valuable tools diverse applications. New expansions the toolbox signal transduction, genome editing, “painting” patterns active molecules onto membranes, light‐induced cell cycle control. A combination light‐ chemically dimerization also seen interesting progress. Herein, an overview emerging provided, recent applications tackling complex problems are discussed.

Language: Английский

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