Biosystem‐Inspired Engineering of Nanozymes for Biomedical Applications

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(10)

Published: Jan. 9, 2023

Abstract Nanozymes with intrinsic enzyme‐mimicking activities have shown great potential to become surrogates of natural enzymes in many fields by virtue their advantages high catalytic stability, ease functionalization, and low cost. However, due the lack predictable descriptors, most nanozymes reported past been obtained mainly through trial‐and‐error strategies, efficacy, substrate specificity, as well practical application effect under physiological conditions, are far inferior that enzymes. To optimize efficacies functions biomedical settings, recent studies introduced biosystem‐inspired strategies into nanozyme design. In this review, advances engineering leveraging refined structure enzymes, simulating behavior changes process, mimicking specific biological processes or living organisms, introduced. Furthermore, currently involved applications summarized. More importantly, current opportunities challenges design discussed. It is hoped based on bioinspired will be beneficial for constructing new generation broadening applications.

Language: Английский

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A Cascade Nanozyme with Amplified Sonodynamic Therapeutic Effects through Comodulation of Hypoxia and Immunosuppression against Cancer

ACS Nano, Journal Year: 2021, Volume and Issue: 16(1), P. 485 - 501

Published: Dec. 28, 2021

The tumor microenvironment (TME) featured by immunosuppression and hypoxia is pivotal to cancer deterioration metastasis. Thus, regulating the TME improve cell ablation efficiency has received extensive interest in oncotherapy. However, reverse alleviate simultaneously are major challenges for effective therapy. Herein, a multifunctional platform based on Au nanoparticles carbon dots modified hollow black TiO2 nanosphere (HABT-C) with intrinsic cascade enzyme mimetic activities prepared reversing alleviating TME. HABT-C NPs possess triple-enzyme activity act as self-cascade nanozymes, which produce sufficient oxygen generate abundant ROS. theoretical analysis demonstrates that facilitates absorption of H2O O2, separation electron–holes, generation ROS, consequently amplifying sonodynamic therapy (SDT) efficiency. Specifically, exhibits favorable inhibition immunosuppressive mediator expression, along infiltrating immune effector cells into As result, can effectively kill via eliciting infiltration, hypoxia, improving SDT This nanozyme-based (HABT-C@HA) will provide strategy highly efficient against modulation

Language: Английский

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Multi-enzyme Co-expressed Dual-Atom Nanozymes Induce Cascade Immunogenic Ferroptosis via Activating Interferon-γ and Targeting Arachidonic Acid Metabolism

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(16), P. 8965 - 8978

Published: April 14, 2023

Immunotherapy is currently the most promising treatment strategy for long-term tumor regression. However, current cancer immunotherapy shows low response rates due to insufficient immunogenicity of cells. Herein, we report a keep cells highly immunogenic by triggering cascade ferroptosis. We developed six-enzyme co-expressed nanoplatform: lipoxygenase (LOX) and phospholipase A2 (PLA2)-co-loaded FeCo/Fe-Co dual-metal atom nanozyme (FeCo/Fe-Co DAzyme/PL), which can not only induce initial ferroptosis through its own multi-enzyme mimetic activities but also up-regulate arachidonic acid (AA) expression synergize with CD8+ T cell-derived IFN-γ ACSL4-mediated During this process, DAzyme/PL lipid peroxidation (LPO) efficiently generating reactive oxygen species (ROS) depleting GSH GPX4 at sites. Additionally, free AA released from PLA2 catalysis converted into arachidonyl-CoA under activation ACSL4 stimulated IFN-γ, further incorporated phospholipids on membranes peroxidized participation LOX. Consequently, promote irreversible multiple ROS storms, GSH/GPX4 depletion, LOX catalysis, IFN-γ-mediated activation, constructing an effective pathway overcome drawbacks immunotherapy.

Language: Английский

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Single‐Atom Nanozyme with Asymmetric Electron Distribution for Tumor Catalytic Therapy by Disrupting Tumor Redox and Energy Metabolism Homeostasis

Advanced Materials, Journal Year: 2022, Volume and Issue: 35(9)

Published: Nov. 14, 2022

Nanozyme catalytic therapy triggered by tumor-specific endogenous stimuli is an emerging tumor that attracts wide attention. However, the current therapeutic efficacy of nanozyme severely limited efficiency nanozymes and concentration reaction substrates. Herein, a novel efficient IrN5 single-atom (IrN5 SA) developed with multiple enzyme-like activities. Due to synergistic effect central Ir axial N coordination, SA exhibits better enzymatic performance than IrN4 SA. At sites, can generate large amount reactive oxygen species (ROS) through oxidase (OXD)-like peroxidase (POD)-like Moreover, also O2 hydrogen peroxide (H2 ) catalase (CAT)-like nicotinamide adenine dinucleotide (NADH) (NOX)-like activities, realizing in substrate-cycle manner. Additionally, effectively break intracellular NADH/NAD+ cycle balance mimicking NOX, then cooperate fatty acid synthase cerulenin (Cer) interfere energy metabolism homeostasis cells. Consequently, designed SA/Cer nanoagent disrupt redox metabolic region enzyme-mimicking cascade reaction, overcoming shortcomings therapy.

Language: Английский

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POD Nanozyme optimized by charge separation engineering for light/pH activated bacteria catalytic/photodynamic therapy

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: March 28, 2022

Abstract The current feasibility of nanocatalysts in clinical anti-infection therapy, especially for drug-resistant bacteria infection is extremely restrained because the insufficient reactive oxygen generation. Herein, a novel Ag/Bi 2 MoO 6 (Ag/BMO) nanozyme optimized by charge separation engineering with photoactivated sustainable peroxidase-mimicking activities and NIR-II photodynamic performance was synthesized solvothermal reaction photoreduction. Ag/BMO held satisfactory bactericidal against methicillin-resistant Staphylococcus aureus (MRSA) (~99.9%). excellent antibacterial NPs ascribed to corporation peroxidase-like activity, behavior, acidity-enhanced release Ag + . As revealed theoretical calculations, introduction BMO made it easier separate photo-triggered electron-hole pairs ROS production. And conduction valence band potentials were favorable reduction O ·O − Under 1064 nm laser irradiation, electron transfer beneficial reversible change Mo 5+ /Mo 6+ , further improving catalytic activity based on Russell mechanism. In vivo, exhibited promising therapeutic effects towards MRSA-infected wounds. This study enriches research proves that nanozymes can be rationally strategy.

Language: Английский

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Bioactive Iridium Nanoclusters with Glutathione Depletion Ability for Enhanced Sonodynamic‐Triggered Ferroptosis‐Like Cancer Cell Death

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(45)

Published: Sept. 22, 2022

Ferroptosis is a regulated form of necrotic cell death that involves the accumulation lipid peroxide (LPO) species in an iron- and reactive oxygen (ROS)-dependent manner. Previous investigations have reported ferroptosis-based cancer therapy can overcome limitations traditional therapeutics targeting apoptosis pathway. However, it still challenging to enhance antitumor efficacy ferroptosis due intrinsic cellular regulation. In this study, ferroptosis-inducing agent, i.e., chlorin e6 (Ce6)-conjugated human serum albumin-iridium oxide (HSA-Ce6-IrO2 , HCIr) nanoclusters, developed achieve sonodynamic (SDT)-triggered ferroptosis-like death. The sonosensitizing role both Ce6 IrO2 within HCIr nanoclusters exhibits highly efficient 1 O2 generation capacity upon ultrasound stimulation, which promotes LPO subsequently induces ferroptosis. Meanwhile, deplete glutathione (GSH) by accelerating Ir (IV)-Ir (III) transition, further suppresses activity peroxidase 4 (GPX4) efficacy. Through vitro vivo experiments, demonstrated possesses tremendous reduce intracellular GSH content, enhances SDT-triggered Thus, iridium-nanoclusters-based agent developed, providing promising strategy for inducing

Language: Английский

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Redox dyshomeostasis strategy for tumor therapy based on nanomaterials chemistry

Chemical Science, Journal Year: 2022, Volume and Issue: 13(8), P. 2202 - 2217

Published: Jan. 1, 2022

This review summarizes the current progress of redox dyshomeostasis (RDH) strategy for tumor therapy. makes cells more sensitive to therapy patterns through using nanomaterials disrupt homeostasis.

Language: Английский

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Breaking the pH Limitation of Nanozymes: Mechanisms, Methods, and Applications

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(31)

Published: April 14, 2024

Although nanozymes have drawn great attention over the past decade, activities of peroxidase-like, oxidase-like, and catalase-like are often pH dependent with elusive mechanism, which largely restricts their application. Therefore, a systematical discussion on pH-related catalytic mechanisms together methods to overcome this limitation is in need. In review, various exhibiting pH-dependent collected root causes for dependence comprehensively analyzed. Subsequently, regulatory concepts including environment reconstruction direct activity improvement break restriction summarized. Moreover, applications pH-independent sensing, disease therapy, pollutant degradation overviewed. Finally, current challenges future opportunities development suggested. It anticipated that review will promote further design broaden application range higher efficiency.

Language: Английский

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Designing nanozymes for in vivo applications

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(10), P. 849 - 868

Published: July 18, 2024

Language: Английский

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Antioxidant and Prooxidant Nanozymes: From Cellular Redox Regulation to Next‐Generation Therapeutics

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(33)

Published: April 21, 2023

Nanozymes, nanomaterials with enzyme-mimicking activity, have attracted tremendous interest in recent years owing to their ability replace natural enzymes various biomedical applications, such as biosensing, therapeutics, drug delivery, and bioimaging. In particular, the nanozymes capable of regulating cellular redox status by mimicking antioxidant mammalian cells are great therapeutic significance oxidative-stress-mediated disorders. As distinction physiological oxidative stress (oxidative eustress) pathological distress) occurs at a fine borderline, it is challenge design that can differentially sense two extremes cells, tissues organs mediate appropriate chemical reactions. this Review, we summarize advances development redox-active applications. We primarily highlight prooxidant disease model systems, cancer, neurodegeneration, cardiovascular diseases. The future perspectives emerging area research challenges associated applications described.

Language: Английский

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