Science Advances,
Journal Year:
2022,
Volume and Issue:
8(26)
Published: June 29, 2022
DNA
aptamers
have
been
widely
used
as
biosensors
for
detecting
a
variety
of
targets.
Despite
decades
success,
they
not
applied
to
monitor
any
targets
in
plants,
even
though
plants
are
major
platform
providing
oxygen,
food,
and
sustainable
products
ranging
from
energy
fuels
chemicals,
high-value
such
pharmaceuticals.
A
barrier
progress
is
lack
efficient
methods
deliver
into
plant
cells.
We
herein
report
thiol-mediated
uptake
method
that
more
efficiently
delivers
Arabidopsis
tobacco
leaf
cells
than
another
state-of-the-art
method,
nanostructures.
Such
allowed
delivery
glucose
aptamer
sensor
sensing
glucose.
This
demonstration
opens
new
avenue
apply
sensors
functional
studies
various
targets,
including
metabolites,
hormones,
metal
ions,
proteins
better
understanding
the
biodistribution
regulation
these
species
their
functions.
Chemical Society Reviews,
Journal Year:
2021,
Volume and Issue:
50(16), P. 8954 - 8994
Published: Jan. 1, 2021
This
article
provides
a
comprehensive
review
of
biosensing
with
DNAzymes,
providing
an
overview
different
sensing
applications
while
highlighting
major
progress
and
seminal
contributions
to
the
field
portable
biosensor
devices
point-of-care
diagnostics.
Specifically,
functional
nucleic
acids
is
introduced,
specific
focus
on
DNAzymes.
The
incorporation
DNAzymes
into
bioassays
then
described,
followed
by
detailed
recent
advances
in
development
vivo
platforms
sensors
incorporating
for
molecular
recognition.
Finally,
critical
perspective
field,
summary
where
DNAzyme-based
may
make
biggest
impact
are
provided.
Nature Reviews Bioengineering,
Journal Year:
2023,
Volume and Issue:
1(8), P. 560 - 575
Published: May 11, 2023
Body-based
biomolecular
sensing
systems,
including
wearable,
implantable
and
consumable
sensors
allow
comprehensive
health-related
monitoring.
Glucose
have
long
dominated
wearable
bioanalysis
applications
owing
to
their
robust
continuous
detection
of
glucose,
which
has
not
yet
been
achieved
for
other
biomarkers.
However,
access
diverse
biological
fluids
the
development
reagentless
approaches
may
enable
design
body-based
systems
various
analytes.
Importantly,
enhancing
selectivity
sensitivity
is
essential
biomarker
in
complex
physiological
conditions.
In
this
Review,
we
discuss
signal
amplification
sensors,
techniques
overcome
Debye
mass
transport
limitations,
improvement,
such
as
integration
artificial
affinity
recognition
elements.
We
highlight
that
can
sequential
real-time
measurements,
example,
implementation
thin-film
transistors
devices.
addition
sensor
construction,
careful
consideration
physical,
psychological
security
concerns
related
required
ensure
transition
from
laboratory
human
body
seamless
possible.
Continuous
monitoring
signatures
potential
transform
our
understanding
personalized
preventative
medicine.
This
Review
Article
discusses
emerging
trends
pertinent
considerations
a
new
generation
vivo
measurement.
Molecular Cancer,
Journal Year:
2023,
Volume and Issue:
22(1)
Published: Feb. 16, 2023
Abstract
Current
clinical
tools
for
breast
cancer
(BC)
diagnosis
are
insufficient
but
liquid
biopsy
of
different
bodily
fluids
has
recently
emerged
as
a
minimally
invasive
strategy
that
provides
real-time
snapshot
tumour
biomarkers
early
diagnosis,
active
surveillance
progression,
and
post-treatment
recurrence.
Extracellular
vesicles
(EVs)
nano-sized
membranous
structures
50–1000
nm
in
diameter
released
by
cells
into
biological
fluids.
EVs
contain
proteins,
nucleic
acids,
lipids
which
play
pivotal
roles
tumourigenesis
metastasis
through
cell-to-cell
communication.
Proteins
miRNAs
from
small
(sEV),
range
size
50–150
nm,
being
investigated
potential
source
novel
BC
using
mass
spectrometry-based
proteomics
next-generation
sequencing.
This
review
covers
recent
developments
sEV
isolation
single
analysis
technologies
summarises
the
protein
miRNA
identified
prognosis,
chemoresistance.
The
limitations
current
biomarker
research
discussed
along
with
future
perspective
applications.
Journal of the American Chemical Society,
Journal Year:
2023,
Volume and Issue:
145(13), P. 7540 - 7547
Published: March 22, 2023
The
classical
DNA
aptamer
for
adenosine
and
ATP
has
been
the
most
used
small
molecule
binding
biosensing,
imaging,
nanotechnology.
This
sequence
recurred
multiple
times
in
previous
selections,
all
selections
a
high
concentration
of
as
target.
Herein,
two
separate
were
performed
using
targets.
By
pushing
target
concentrations
down
to
low
micromolar
range,
new
aptamers
with
Kd
230
nM
obtained,
showing
around
30-fold
higher
affinity
compared
aptamer.
still
dominated
library
early
rounds
but
it
was
suppressed
later
rounds.
bind
one
instead
two.
Mutation
studies
confirmed
their
secondary
structures
specific
binding.
Using
deep
sequencing
data
from
long-standing
questions
such
existence
one-site
mutation
distribution
addressed.
Comparisons
made
previously
reported
ATP.
Finally,
strand-displacement
biosensor
tested
selectivity
its
nucleotides.
Science,
Journal Year:
2023,
Volume and Issue:
380(6648), P. 942 - 948
Published: June 1, 2023
Aptameric
receptors
are
important
biosensor
components,
yet
our
ability
to
identify
them
depends
on
the
target
structures.
We
analyzed
contributions
of
individual
functional
groups
small
molecules
binding
within
27
target-aptamer
pairs,
identifying
potential
hindrances
receptor
isolation-for
example,
negative
cooperativity
between
sterically
hindered
groups.
To
increase
probability
aptamer
isolation
for
targets,
such
as
leucine
and
voriconazole,
which
multiple
previous
selection
attempts
failed,
we
designed
tailored
strategies
focused
overcoming
structural
barriers
successful
selections.
This
approach
enables
us
move
beyond
standardized
protocols
into
group-guided
searches,
relying
sequences
common
targets
their
analogs
serve
anchors
in
regions
vast
oligonucleotide
spaces
wherein
useful
reagents
likely
be
found.
Chemical Reviews,
Journal Year:
2023,
Volume and Issue:
123(22), P. 12471 - 12506
Published: Nov. 6, 2023
Serving
as
the
basis
of
cell
life,
interactions
between
nucleic
acids
and
proteins
play
essential
roles
in
fundamental
cellular
processes.
Aptamers
are
unique
single-stranded
oligonucleotides
generated
by
vitro
evolution
methods,
possessing
ability
to
interact
with
specifically.
Altering
structure
aptamers
will
largely
modulate
their
further
affect
related
behaviors.
Recently,
in-depth
research
aptamer–protein
interactions,
analytical
assays
based
on
have
been
widely
developed
become
a
powerful
tool
for
biomolecular
detection.
There
some
insightful
reviews
applied
protein
detection,
while
few
systematic
discussions
from
perspective
regulating
interactions.
Herein,
we
comprehensively
introduce
methods
elaborate
detection
techniques
analyzing
Additionally,
this
review
provides
broad
summary
regulation
detecting
biomolecules.
Finally,
present
our
perspectives
regarding
opportunities
challenges
biological
analysis,
aiming
provide
guidance
disease
mechanism
drug
discovery.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(16)
Published: Jan. 23, 2024
Abstract
Systematic
evolution
of
ligands
by
exponential
enrichment
(SELEX)
has
been
used
to
discover
thousands
aptamers
since
its
development
in
1990.
Aptamers
are
short
single‐stranded
oligonucleotides
capable
binding
targets
with
high
specificity
and
selectivity
through
structural
recognition.
While
offer
advantages
over
other
molecular
recognition
elements
such
as
their
ease
production,
smaller
size,
extended
shelf‐life,
lower
immunogenicity,
they
have
yet
show
significant
success
real‐world
applications.
By
analyzing
the
importance
structured
library
designs,
reviewing
different
SELEX
methodologies,
effects
chemical
modifications,
we
provide
a
comprehensive
overview
on
production
for
applications
drug
delivery
systems,
therapeutics,
diagnostics,
imaging.
