Published: Jan. 1, 2025
Language: Английский
Small, Journal Year: 2023, Volume and Issue: 20(14)
Published: Nov. 20, 2023
Abstract Nanozymes, as innovative materials, have demonstrated remarkable potential in the field of electrochemical biosensors. This article provides an overview mechanisms and extensive practical applications nanozymes First, definition characteristics are introduced, emphasizing their significant role constructing efficient sensors. Subsequently, several common categories nanozyme materials delved into, including metal‐based, carbon‐based, metal‐organic framework, layered double hydroxide nanostructures, discussing Regarding mechanisms, two key roles particularly focused biosensors: selective enhancement signal amplification, which crucially support sensor performance. In terms applications, widespread use nanozyme‐based biosensors showcased various domains. From detecting biomolecules, pollutants, nucleic acids, proteins, to cells, providing robust means for high‐sensitivity detection. Furthermore, insights into future development is provided, encompassing improvements optimizations design integration, expansion application fields through interdisciplinary collaboration. conclusion, this systematically presents biosensors, offering valuable references prospects research field.
Language: Английский
Citations
63
ECS Sensors Plus, Journal Year: 2024, Volume and Issue: 3(1), P. 011601 - 011601
Published: March 1, 2024
There is a plethora of electrochemical biosensors developed for ultrasensitive detection clinically relevant biomarkers. However, many these systems lose their performance in heterogeneous clinical samples and are too complex to be operated by end users at the point-of-care (POC), prohibiting commercial success. Integration with sample processing technology addresses both challenges; however, it adds manufacturing complexity overall cost systems. Herein, we review different components biosensor avenues creating fully integrated In context integration, focus on discussing trade-offs between sensing performance, cost, scalable guide readers toward designing new commercialization potential.
Language: Английский
Citations
41
Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(15), P. 7960 - 7982
Published: Jan. 1, 2024
This review discusses the advancements, sensor design, and challenges in creating wearable implantable nucleic acid-based sensors for personalized healthcare through real-time biomarker analysis biofluids.
Language: Английский
Citations
28
Biosensors, Journal Year: 2023, Volume and Issue: 13(8), P. 823 - 823
Published: Aug. 15, 2023
Hydrogel-based wearable electrochemical biosensors (HWEBs) are emerging biomedical devices that have recently received immense interest. The exceptional properties of HWEBs include excellent biocompatibility with hydrophilic nature, high porosity, tailorable permeability, the capability reliable and accurate detection disease biomarkers, suitable device–human interface, facile adjustability, stimuli responsive to nanofiller materials. Although biomimetic three-dimensional hydrogels can immobilize bioreceptors, such as enzymes aptamers, without any loss in their activities. However, most suffer from low mechanical strength electrical conductivity. Many studies been performed on electroactive nanofillers, including biomacromolecules, carbon-based materials, inorganic organic nanomaterials, tackle these issues. Non-conductive even conductive may be modified by well redox species. All modifications led design development efficient nanocomposites biosensors. In this review, both conductive-based non-conductive-based derived natural synthetic polymers systematically reviewed. main synthesis methods characterization techniques addressed. behavior discussed detail. Finally, prospects potential applications biosensing, healthcare monitoring, clinical diagnostics highlighted.
Language: Английский
Citations
25
Nano-Micro Letters, Journal Year: 2023, Volume and Issue: 16(1)
Published: Dec. 12, 2023
In vivo monitoring of animal physiological information plays a crucial role in promptly alerting humans to potential diseases animals and aiding the exploration mechanisms underlying human diseases. Currently, implantable electrochemical microsensors have emerged as prominent area research. These not only fulfill technical requirements for but also offer an ideal platform integration. They been extensively studied their ability monitor minimally invasive manner, characterized by bloodless, painless features, exceptional performance. The development has witnessed significant scientific technological advancements through dedicated efforts. This review commenced with comprehensive discussion construction microsensors, including materials utilized methods employed fabrication. Following this, we proceeded explore various implantation technologies microsensors. addition, overview was provided applications specifically investigation disease mechanisms. Lastly, concise conclusion conducted on recent obstacles pertaining practical implementation
Language: Английский
Citations
25
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(18), P. 7172 - 7178
Published: April 22, 2024
Achieving sensitive detection and accurate identification of cancer cells is vital for diagnosing treating the disease. Here, we developed a logic signal amplification system using DNA tetrahedron-mediated three-dimensional (3D) nanonetworks electrochemiluminescence (ECL) subtype cells. Specially designed hairpins were integrated into tetrahedral nanostructures (DTNs) to perform catalytic hairpin assembly (CHA) reaction in presence target microRNA, forming hyperbranched 3D nanonetworks. Benefiting from "spatial confinement effect," (DTCHA) displayed significantly faster kinetics greater cycle conversion efficiency than traditional CHA. The resulting could load large amount Ru(phen)
Language: Английский
Citations
13
Critical Reviews in Clinical Laboratory Sciences, Journal Year: 2024, Volume and Issue: 61(6), P. 473 - 495
Published: March 7, 2024
Nucleic acids, like DNA and RNA, serve as versatile recognition elements in electrochemical biosensors, demonstrating notable efficacy detecting various cancer biomarkers with high sensitivity selectivity. These biosensors offer advantages such cost-effectiveness, rapid response, ease of operation, minimal sample preparation. This review provides a comprehensive overview recent developments nucleic acid-based for diagnosis, comparing them antibody-based counterparts. Specific examples targeting key biomarkers, including prostate-specific antigen, microRNA-21, carcinoembryonic are highlighted. The discussion delves into challenges limitations, encompassing stability, reproducibility, interference, standardization issues. suggests future research directions, exploring new acid elements, innovative transducer materials designs, novel signal amplification strategies, integration microfluidic devices or portable instruments. Evaluating these clinical settings using actual samples from patients healthy donors is emphasized. sensors sensitive specific at non-communicable communicable disease biomarkers. RNA's self-assembly, programmability, catalytic activity, dynamic behavior enable adaptable sensing platforms. They can increase biosensor biocompatibility, transduction, nanomaterials. In conclusion, acids-based hold significant potential to enhance detection treatment through early accurate diagnosis.
Language: Английский
Citations
10
ACS Nano, Journal Year: 2024, Volume and Issue: 18(46), P. 31713 - 31736
Published: Nov. 7, 2024
Sensitive and quantitative detection of chemical biological molecules for screening, diagnosis monitoring diseases is essential to treatment planning response monitoring. Electrochemical biosensors are fast, sensitive, easy miniaturize, which has led rapid development in clinical diagnosis. Benefiting from their excellent molecular recognition ability high programmability, DNA nanomaterials could overcome the Debye length electrochemical by simple design well suited as elements biosensors. Therefore, enhance sensitivity specificity biosensors, significant progress been made recent years optimizing design. Here, establishment sensing strategies based on reviewed detail. First, structural nanomaterial examined improving overcoming length. In addition, electrical signal transduction amplification reviewed, applications nanomaterial-based integrated devices further summarized. Finally, main opportunities challenges detecting disease biomarkers presented an aim guide with specificity.
Language: Английский
Citations
10
ACS Measurement Science Au, Journal Year: 2023, Volume and Issue: 3(6), P. 404 - 433
Published: Oct. 27, 2023
The critical performance factors such as selectivity, sensitivity, operational and storage stability, response time of electrochemical biosensors are governed mainly by the function their key component, bioelectrode. Suitable design fabrication strategies bioelectrode interface essential for realizing requisite practical utility. A multifaceted attempt to achieve this goal is visible from vast literature exploring effective preparing, immobilizing, stabilizing biorecognition elements on electrode surface efficient transduction biochemical signals into electrical ones (i.e., current, voltage, impedance) through with aid advanced materials techniques. commercial success in modern society also increasingly influenced size (and hence portability), multiplexing capability, coupling wireless communication technology, which facilitates quick data transfer linked decision-making processes real-time different areas healthcare, agriculture, food, environmental applications. Therefore, involves careful selection control several parameters, including elements, materials, shape electrode, detection principles, various strategies, microscale printing technologies. This review discusses recent trends designs fabrications developing biosensors. discussions have been delineated types immobilization signal approaches, commonly used techniques fabricating bioelectrodes, device integration electronic technology interest.
Language: Английский
Citations
20
Advanced Science, Journal Year: 2023, Volume and Issue: 10(19)
Published: April 23, 2023
Abstract There is a need for point‐of‐care bacterial sensing and identification technologies that are rapid simple to operate. Technologies do not rely on growth cultures, nucleic acid amplification, step‐wise reagent addition, complex sample processing the key meeting this need. Herein, multiple materials integrated overcoming obstacles in creating one‐pot platforms. Liquid‐infused nanoelectrodes developed reducing nonspecific binding transducer surface; bacterium‐specific RNA‐cleaving DNAzymes used identification; redox DNA barcodes embedded into binding‐induced electrochemical signal transduction. The resultant single‐step assay demonstrates limit‐of‐detection of 10 2 CFU mL −1 , with high specificity identifying Escherichia coli amongst other Gram positive negative bacteria including Klebsiella pneumoniae Staphylococcus aureus Bacillus subtilis . Additionally, evaluated analyzing 31 clinically obtained urine samples, demonstrating clinical sensitivity 100% specify 100%. When challenging nine blood E. ‐positive ‐negative samples can be distinguished probability p < 0.001.
Language: Английский
Citations
19