Ultrasensitive Detection of FEN1 Activity for Cancer Diagnosis Using a CRISPR/Cas13a‐Based Triple Cascade Amplification System DOI

Xia Cheng,

Wenchen Zhao,

Daixi Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

Flap endonuclease 1 (FEN1) is closely associated with tumor progression and proliferation, making it a promising biomarker for cancer diagnosis. However, developing sensitive, reliable, user-friendly method quantitative FEN1 detection remains technically challenging. In this study, an ultrasensitive biosensor established using target-induced cleavage-ligation-transcription-activation cascade strategy (LTACas13a) to enhance the cleavage ability of CRISPR/Cas13a. The LTACas13a has shown excellent performance in screening inhibitors detecting endogenous activity living cells, as well clinical biological samples such human serum tissue samples. Additionally, universal dumbbell probe derived from FEN1, multiplex developed various DNA glycosylases, including formamidopyrimidine glycosylase, uracil alkyl adenine glycosylase. This straightforward approach provides reliable effective diagnostic tool early-stage offers significant opportunities biosensing related drug discovery.

Language: Английский

CRISPR-Based Homogeneous Electrochemical Strategy for Near-Zero Background Detection of Breast Cancer Extracellular Vesicles via Fluidity-Enhanced Magnetic Capture Nanoprobe DOI
Limin Yang, Jingang Zhang, Jing Zhang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Precise identification and analysis of multiple protein biomarkers on the surface breast cancer cell-derived extracellular vesicles (BC-EVs) are great significance for noninvasive diagnosis subtypes, but it remains a major challenge owing to their high heterogeneity low abundance. Herein, we established CRISPR-based homogeneous electrochemical strategy near-zero background ultrasensitive detection BC-EVs. To realize high-performance capture isolation BC-EVs, fluidity-enhanced magnetic nanoprobes were facilely prepared. After capturing AND logic gate-based catalytic hairpin assembly (CHA) trans-cleavage activity CRISPR-Cas12a against signal triggered successively, generating significant signal. Notably, as-developed metal-mediated could efficiently decrease by separation, endowing method with signal-to-noise ratio. Consequently, ingeniously integrating DNA CRISPR-CHA amplification dual in strategy, precise BC-EVs was successfully achieved through simultaneous specific recognition markers surface. More importantly, this approach effectively discriminate subgroups clinical serum samples, which may provide opportunities accurate prognosis evaluation manner.

Language: Английский

Citations

2
Functional Nucleic Acid Nanostructures for Mitochondrial Targeting: The Basis of Customized Treatment Strategies DOI Creative Commons
Wanchong He,

Siyu Dong,

Qinghua Zeng

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1025 - 1025

Published: Feb. 24, 2025

Mitochondria, as vital organelles, play a central role in subcellular research and biomedical innovation. Although functional nucleic acid (FNA) nanostructures have witnessed remarkable progress across numerous biological applications, strategies specifically tailored to target mitochondria for molecular imaging therapeutic interventions remain scarce. This review delves into the latest advancements leveraging FNA mitochondria-specific cancer therapy. Initially, we explore creation of FNA-based biosensors localized mitochondria, enabling real-time detection visualization critical molecules essential mitochondrial function. Subsequently, examine developments aimed at mitochondrial-targeted treatments, including modular nanodevices precise delivery agents programmable disrupting processes. Emphasis is placed on elucidating chemical principles underlying design mitochondrial-specific nanotechnology diverse uses. Lastly, address unresolved challenges outline prospective directions, with goal advancing field encouraging sophisticated tools both academic inquiry clinical applications centered mitochondria.

Language: Английский

Citations

0
Recent advances on DNAzyme for bioimaging, biosensing and cancer therapy DOI
Pei Sun,

Hongquan Gou,

Xinran Che

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(78), P. 10805 - 10821

Published: Jan. 1, 2024

DNAzymes, a class of single-stranded catalytic DNA with good stability, high activity, and easy synthesis, functionalization modification properties, have garnered significant interest in the realm biosensing bioimaging. Their integration fluorescent dyes or chemiluminescent moieties has led to remarkable bioimaging outcomes, while DNAzyme-based biosensors demonstrated robust sensitivity selectivity detecting metal ions, nucleic acids, proteins, enzyme activities, exosomes, bacteria microorganisms. In addition, by delivering DNAzymes into tumor cells, mRNA therein can be cleaved regulate expression corresponding which further propelled application cancer gene therapy synergistic therapy. This paper reviews strategies for screening attractive such as SELEX high-throughput sequencing, briefly describes amplification mainly include hairpin assembly (CHA), walker, hybridization chain reaction (HCR), origami, CRISPR-Cas12a, rolling circle (RCA), aptamers. applications bioimaging, biosensing, are also highlighted. Subsequently, possible challenges these practical pointed out, future research directions suggested.

Language: Английский

Citations

4
Fusing Allosteric Ribozymes with CRISPR‐Cas12a for Efficient Diagnostics of Small Molecule Targets DOI Open Access
Lichuan Guo, Shu Zhang,

Xinyu Du

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract The CRISPR‐Cas systems are adopted as powerful molecular tools for not only genetic manipulation but also point‐of‐care diagnostics. However, methods to enable diagnostics of non‐nucleic‐acid targets with these still limited. Herein, by fusing ligand‐dependent allosteric ribozymes CRISPR‐Cas12a, a derived system is created efficient quantitative analysis in 1–2 h. On two different small molecules, the system's generality, reliability and accuracy demonstrated, show that well operability this can high‐throughput detection molecule blood samples. be further converted rely on deoxyribozyme instead ribozyme recognize transduce signal CRISPR‐Cas12a amplification, likely making it easier storage more consistent data generation DNA possess stability advantage over RNA. This (deoxy)ribozyme‐assisted anticipates facilitate bioanalysis various scientific clinical settings drive development translation.

Language: Английский

Citations

4
Frontiers in Fluorescence imaging: Tools for the In-Situ Sensing of Disease Biomarkers DOI
Lei Yang, Hongwei Hou, Jinghong Li

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

A comprehensive overview of recent advancements in fluorescence imaging techniques for situ sensing various biomarkers, emphasizing the transformative potential artificial intelligence shaping future bioimaging.

Language: Английский

Citations

4
Biomineralized and metallized small extracellular vesicles encapsulated in hydrogels for mitochondrial-targeted synergistic tumor therapy DOI
Qi Zhang,

Ruofei Ma,

Siwen Chen

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Functional Nucleic Acid-based Fluorescence Imaging for Tumor Microenvironment Monitoring: A Review DOI
Zuoxiang Liang, Fengyu Tian

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: 1350, P. 343794 - 343794

Published: Feb. 13, 2025

Language: Английский

Citations

0
Subcellular Compartment‐Specific Amplified Imaging of Metal Ions via Ribosomal RNA‐Regulated DNAzyme Sensors DOI

Deyu Yi,

Lele Li, Mengyuan Li

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

Abstract Although DNAzyme sensors have been widely developed for imaging metal ions, their application in specific subcellular compartments remains challenging due to low spatial controllability. Here we present a locally activatable, DNAzyme‐based sensing technology that enables compartment‐specific of ions through ribosomal RNA (rRNA) regulated signal amplification. The system leverages subcellularly encoded rRNA activate sensors, and further drives amplification via multiple turnover cleavage molecular beacons, significantly enhance sensitivity precision metal‐ion organelles (e.g. mitochondria) or membraneless cytosol). Furthermore, demonstrate the allows situ monitoring dynamics mitochondrial Zn 2+ during ischemia drug intervention. This study expands toolbox investigating role disease processes.

Language: Английский

Citations

3
Visualizing DNA/RNA, Proteins, and Small Molecule Metabolites within Live Cells DOI
Dongling Jia,

Minhui Cui,

Xianting Ding

et al.

Small, Journal Year: 2024, Volume and Issue: 20(46)

Published: Aug. 3, 2024

Live cell imaging is essential for obtaining spatial and temporal insights into dynamic molecular events within heterogeneous individual cells, in situ intracellular networks, vivo organisms. Molecular tracking live cells also a critical general requirement studying physiological processes biology, cancer, developmental neuroscience. Alongside this context, review provides comprehensive overview of recent research progress live-cell RNAs, DNAs, proteins, small-molecule metabolites, as well their applications diagnosis, immunodiagnosis, biochemical diagnosis. A series advanced techniques have been introduced summarized, including high-precision imaging, high-resolution low-abundance multidimensional multipath rapid computationally driven methods, all which offer valuable disease prevention, treatment. This article addresses the current challenges, potential solutions, future development prospects field.

Language: Английский

Citations

2
Subcellular Compartment‐Specific Amplified Imaging of Metal Ions via Ribosomal RNA‐Regulated DNAzyme Sensors DOI Open Access

Deyu Yi,

Lele Li, Mengyuan Li

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

Although DNAzyme sensors have been widely developed for imaging metal ions, their application in specific subcellular compartments remains challenging due to low spatial controllability. Here we present a locally activatable, DNAzyme-based sensing technology that enables compartment-specific of ions through ribosomal RNA (rRNA) regulated signal amplification. The system leverages subcellularly encoded rRNA activate sensors, and further drives amplification via multiple turnover cleavage molecular beacons, significantly enhance sensitivity precision metal-ion organelles (e.g. mitochondria) or membraneless cytosol). Furthermore, demonstrate the allows situ monitoring dynamics mitochondrial Zn

Language: Английский

Citations

2