Chemically modified DNAzymes-based electrochemical sensor for binary and highly sensitive detection of reactive oxygen species

Chemical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

A chemically modified DNAzyme-based electrochemical sensor was developed for binary and highly sensitive detection of reactive oxygen species.

Language: Английский

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Selection of High-Affinity and Selectivity AFB₁ Circular Aptamer for Biosensor Application

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Detecting trace amounts of aflatoxin B1 (AFB1), one the most toxic food contaminants, is crucial for efficiently preventing potential health risks. Circular aptamers are promising candidates bioanalytical applications due to their enhanced biological and structural stability as well compatibility with rolling circle amplification (RCA). Herein, we employed a high-efficiency magnetic chain graphene oxide-based SELEX generate circular that bind AFB1 high affinity selectivity. Notably, selected aptamer, CAFB1-A-2, exhibited significant sequence similarity classical linear aptamer but demonstrated distinct thermodynamic mechanism binding. The binding CAFB1-A-2 was driven by both enthalpy entropy, whereas notable entropy loss required greater compensation. Utilizing this developed sensitive detection system featuring an RCA-assisted allosteric DNAzyme biosensor limit (LOD) 265 pM. Furthermore, constructed aptasensor successfully detected in spiked rice corn, achieving LODs 11 9 μg/kg, respectively, within approximately 4 h. This study provides reliable alternative development AFB1, holding great field safety detection.

Language: Английский

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A Fully Integrated ICP‐Enriched and Nanozyme‐Catalyzed Lateral Flow Assay for cfDNA Detection in Whole Blood

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Rapid and sensitive detection of Epstein−Barr virus cell‐free DNA (EBV cfDNA) is crucial for early diagnosis monitoring nasopharyngeal carcinoma (NPC), but accessibility to screening limited by complicated costly conventional isolation purification approaches. Here, a fully integrated ion concentration polarization (ICP)‐enriched nanozyme‐catalyzed lateral flow assay (ICP‐cLFA) developed, enabling total analysis EBV cfDNA in whole blood samples, with isolation, pre‐concentration, amplification performed on microfluidic chip, consequently providing the signal readout within 75 min. Specifically, ICP preconcentration steps, together target recognition catalyzed platinum‐decorated mesoporous gold nanosphere (MGNS@Pt) nanozyme, result an ultralow limit 4 aM standard samples 100 from NPC‐bearing rats. The high sensitivity specificity ICP‐cLFA suggest strong potential resource‐limited clinical field applications.

Language: Английский

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Reaction Pathway Differentiation Enabled Fingerprinting Signal for Single Nucleotide Variant Detection

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Abstract Accurate identification of single‐nucleotide variants (SNVs) is paramount for disease diagnosis. Despite the facile design DNA hybridization probes, their limited specificity poses challenges in clinical applications. Here, a differential reaction pathway probe (DRPP) based on dynamic network presented. DRPP leverages differences intermediate concentrations between SNV and WT groups, directing them into distinct pathways. This generates strong pulse‐like signal weak unidirectional increase wild‐type (WT). Through application machine learning to fluorescence kinetic data analysis, classification signals automated with an accuracy 99.6%, significantly exceeding 80.7% conventional methods. Additionally, sensitivity variant allele frequency (VAF) enhanced down 0.1%, representing ten‐fold improvement over approaches. accurately identified D614G N501Y SNVs S gene SARS‐CoV‐2 patient swab samples 99% (n = 82). It determined VAF ovarian cancer‐related mutations KRAS‐G12R , NRAS‐G12C BRAF‐V600E both tissue blood 77), discriminating cancer patients healthy individuals significant difference ( p < 0.001). The potential integration diagnostics, along rapid amplification techniques, holds promise early diagnostics personalized diagnostics.

Language: Английский

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A PEG-based strategy to improve detection of clinical microRNA 155 by bio-Field Effect Transistor in high ionic strength environment

Talanta, Journal Year: 2025, Volume and Issue: 292, P. 127881 - 127881

Published: March 11, 2025

microRNAs are small oligonucleotides involved in post-transcriptional gene regulation whose alteration is found several diseases, including cancer, and therefore their detection crucial for diagnosis, prognosis, treatment purposes. Field-Effect Transistor-based biosensors (bioFETs) represent a promising technology the clinical of microRNAs. However, one main challenges associated with this Debye screening, becoming significant at high ionic strengths required effective hybridization. We aimed detecting oncogenic microRNA-155 by using bioFET system as capture element complementary RNA probe (antimiR-155) combined introduction PEG molecules (20 kDa, PEG20), an strength 300 mM. optimized co-immobilization ratio between antimiR-155 PEG20 assessed its impact on interactions oligonucleotides. The kinetics can be well described Langmuir-Freundlich isotherm affinity constant within range typical nucleic acid interactions. significantly enhanced sensitivity miR-155 reaching level less than 200 pM, together excellent discrimination against other clinically relevant Our findings demonstrate that incorporation constitutes strategy to mitigate screening effects facilitates bioFET-based applications physiological strengths.

Language: Английский

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Engineering Gene-Specific DNAzymes for Accessible and Multiplexed Nucleic Acid Testing

JACS Au, Journal Year: 2024, Volume and Issue: 4(4), P. 1664 - 1672

Published: April 9, 2024

The accurate and timely detection of disease biomarkers at the point-of-care is essential to ensuring effective treatment epidemiological surveillance. Here, we report selection engineering RNA-cleaving DNAzymes that respond specific genetic markers amplify signals. Because target-specific activation gene-specific (gDz) like trans-cleavage activity clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated (Cas) machinery, further developed a CRISPR-like assay using DNAzyme coupled with isothermal sequence signal amplification (CLARISSA) for nucleic acid in clinical samples. Building on high specificity orthogonality gDzs, CLARISSA highly versatile expandable multiplex testing. Upon integration an recombinase polymerase amplification, enabled human papillomavirus (HPV) 16 189 cervical samples collected from cancer screening participants (n = 189) 100% sensitivity 97.4% specificity, respectively. A multiplexed allowed simultaneous analyses HPV16 HPV18 46 samples, which returned 96.3% 83.3% HPV18, No false positives were found throughout our tests. Besides fluorescence readout fluorogenic reporter probes, also demonstrated be fully compatible visual lateral flow readout. sensitivity, accessibility, multiplexity, believe ideal CRISPR-Dx alternative diagnosis field-based applications.

Language: Английский

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Advances in rapid point-of-care virus testing

The Analyst, Journal Year: 2024, Volume and Issue: 149(9), P. 2507 - 2525

Published: Jan. 1, 2024

The researchers detect viruses through various analyses based on three targets: nucleic acids, antigens, and antibodies.

Language: Английский

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A Triple Signal Amplification Strategy for Accurate and Ultrasensitive miRNA-21 Detection

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(36), P. 14464 - 14470

Published: Aug. 26, 2024

A triple signal amplification strategy was integrated with a built-in double electrode and external energy storage device to fabricate novel self-powered biosensor for ultrasensitive detection of miRNA-21. Specifically, DNA tetrahedra haripin2-glucose oxidase are modified on the surface biocathode bioanode by catalytic hairpin assembly (CHA) achieve dual amplification. Moreover, is realized including an capacitor. Consequently, as-constructed demonstrates low limit 0.06 fM toward miRNA-21 assay within range 0.1 10 pM. This study presents practical sensitive approach timely cancer detection.

Language: Английский

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Endogenous Enzyme-Driven Amplified DNA Nanocage Probe for Selective and Sensitive Imaging of Mature MicroRNAs in Living Cancer Cells

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(23), P. 9453 - 9459

Published: May 31, 2024

Selective and sensitive imaging of intracellular mature microRNAs (miRNAs) is great importance for biological process study medical diagnostics. However, this goal remains challenging because the interference precursor miRNAs (pre-miRNAs) low abundance miRNAs. Herein, we develop an endogenous enzyme-driven amplified DNA nanocage probe (Acage) selective in living cells. The Acage consists a microRNA-responsive probe, fuel strand, framework with inner cavity. Benefiting from size selectivity nanocage, smaller rather than larger pre-miRNAs are allowed to enter cavity molecular recognition; thus, can significantly reduce signal pre-miRNAs. Moreover, driving force enzyme apurinic/apyrimidinic endonuclease 1 (APE1) efficient amplification, enables miRNA without additional external intervention. With these features, was successfully applied during drug treatment. We believe that strategy provides promising pathway better understanding functions processes

Language: Английский

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Enhanced enzyme-like DNAzyme@MOFs for non-enzymatic uric acid detection in interstitial fluid

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 156837 - 156837

Published: Oct. 1, 2024

Language: Английский

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