Getah virus triggers ROS-mediated autophagy in mouse Leydig cells DOI Creative Commons
Fengqin Li,

Lishuang Deng,

Tong Xu

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 13, 2025

Introduction Getah virus (GETV) is a zoonotic transmitted via mosquito-vertebrate cycle. While previous studies have explored the epidemiology and pathogenicity of GETV in various species, its molecular mechanisms remain largely unexplored. Methods This study investigated impact infection associated on reactive oxygen species (ROS) autophagy levels mouse Leydig cells both vivo vitro . The male mice TM3 were treatment with N-acetylcysteine (NAC) to reduce cellular ROS levels. Rapamycin (Rapa) 3-Methyladenine (3- MA) used change infected uninfected cells. Results Discussion findings revealed that testes speciffcally targeted induced oxidative stress while enhancing testicular tissue. Using as an model, confirmed replication this cell line, triggering increased Treatment markedly reduced tissue GETV. Interestingly, use rapamycin led cells, no signiffcant alterations These results indicate elevates levels, subsequently inducing We also found plays important role replication. When using NAC 3-MA, corresponding decrease TCID 50 was observed. Conversely, upregulation Rapa resulted increase Therefore, we speculate may exploit pathway facilitate ffndings illuminate interplay between host providing valuable insights for therapeutic strategies targeting infections.

Language: Английский

Celastrol inhibits rheumatoid arthritis by inducing autophagy via inhibition of the PI3K/AKT/mTOR signaling pathway DOI Creative Commons
Junjie Yang, Jiayu Liu, Jing Li

et al.

International Immunopharmacology, Journal Year: 2022, Volume and Issue: 112, P. 109241 - 109241

Published: Sept. 15, 2022

Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disorder of the synovial joints. Celastrol (Cel) quinone-methylated triterpenoid extracted from Tripterygium wilfordii Hook F (TwHF) that has been proven to be effective in treating RA. However, underlying molecular mechanism celastrol treatment RA remains unknown. This study explored protective effect against and specific mechanisms vitro vivo.A chicken type II collagen (CII)-induced (CIA) mouse model was used explore anti-arthritic effects celastrol, paw swelling degree, poly-arthritis index score serum cytokine levels were determined. Pathological morphology observed using hematoxylin eosin (H&E) staining. The influences on proliferation tumor necrosis factor-α (TNF-α)-induced fibroblast-like synoviocytes (FLSs) tested by Cell Counting Kit-8 (CCK-8) assays and5-ethynyl-2'-deoxyuridine (EdU) staining assays. level autophagy detected transmission electron microscopy (TEM). Furthermore, PI3K/AKT/mTOR pathway status CIA FLSs also western blot immunofluorescence staining.The results showed decreased severity inhibited TNF-α-induced proliferation. Additionally, secretion pro-inflammatory cytokines. Moreover, increased autophagosome LC3B protein expression TNF-α-treated FLSs. LC3-II Beclin-1 phosphorylation degree mTOR AKT.In conclusion, our findings confirmed ameliorates via up-regulation inhibiting axis.

Language: Английский

Citations

51
The Impact of Oxidative Stress and AKT Pathway on Cancer Cell Functions and Its Application to Natural Products DOI Creative Commons
Jun‐Ping Shiau,

Ya-Ting Chuang,

Jen‐Yang Tang

et al.

Antioxidants, Journal Year: 2022, Volume and Issue: 11(9), P. 1845 - 1845

Published: Sept. 19, 2022

Oxidative stress and AKT serine-threonine kinase (AKT) are responsible for regulating several cell functions of cancer cells. Several natural products modulate both oxidative anticancer effects. However, the impact product-modulating on lacks systemic understanding. Notably, contribution by downstream effectors is not yet well integrated. This review explores role pathway (AKT/AKT effectors) ten functions, including apoptosis, autophagy, endoplasmic reticulum stress, mitochondrial morphogenesis, ferroptosis, necroptosis, DNA damage response, senescence, migration, cell-cycle progression. The connected to these through function mediators. Moreover, related Based this rationale, with modulating abilities exhibit potential regulate but some were rarely reported, particularly effectors. sheds light understanding roles in providing future directions treatment.

Language: Английский

Citations

42
Targeting the Interplay of Autophagy and ROS for Cancer Therapy: An Updated Overview on Phytochemicals DOI Creative Commons
Lixia Dong,

Jingqiu He,

Li Luo

et al.

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(1), P. 92 - 92

Published: Jan. 8, 2023

Autophagy is an evolutionarily conserved self-degradation system that recycles cellular components and damaged organelles, which critical for the maintenance of homeostasis. Intracellular reactive oxygen species (ROS) are short-lived molecules containing unpaired electrons formed by partial reduction molecular oxygen. It widely known autophagy ROS can regulate each other to influence progression cancer. Recently, due wide potent anti-cancer effects with minimal side effects, phytochemicals, especially those modulate autophagy, have attracted great interest researchers. In this review, we afford overview complex regulatory relationship between in cancer, emphasis on phytochemicals cancer therapy. We also discuss ROS/autophagy inhibitors challenges associated harnessing regulation potential

Language: Английский

Citations

39
Application of 3D- printed hydrogels in wound healing and regenerative medicine DOI Creative Commons
Milena Deptuła, Małgorzata Zawrzykraj, Justyna Sawicka

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 167, P. 115416 - 115416

Published: Sept. 6, 2023

Hydrogels are three-dimensional polymer networks with hydrophilic properties. The modifiable properties of hydrogels and the structure resembling living tissue allow their versatile application. Therefore, increasing attention is focused on use as bioinks for (3D) printing in engineering. Bioprinting involves fabrication complex structures from several types materials, cells, bioactive compounds. Stem cells (SC), such mesenchymal stromal (MSCs) frequently employed 3D constructs. SCs have desirable biological ability to differentiate into various high proliferative capacity. Encapsulating hydrogel constructs enhances reparative abilities improves likelihood reaching target tissues. In addition, created can simulate environment mimic signals. Importantly, immunogenicity scaffolds minimized through patient-specific biocompatibility biodegradability biopolymers. Regenerative medicine taking advantage aforementioned capabilities regenerating tissues- muscle, bones, nerves, heart, skin, cartilage.

Language: Английский

Citations

36
The Effect of Omega-3 Fatty Acids on Insulin Resistance DOI Creative Commons
Susmita Sinha, Mainul Haque, Halyna Lugova

et al.

Life, Journal Year: 2023, Volume and Issue: 13(6), P. 1322 - 1322

Published: June 5, 2023

Insulin resistance is a critical pathophysiological process in the onset and advancement of type 2 diabetes mellitus. It well-recognized that alterations metabolism lipids aberrant fat buildup effectively trigger development to insulin. Adjusting one’s eating habits managing weight appropriately are crucial for treating, controlling, reducing risk T2DM because obesity lack physical exercise primary factors responsible worldwide rise T2DM. Omega-3 fatty acid one polyunsaturated acids (PUFA) include long-chain omega-3 such as eicosapentaenoic docosahexaenoic acid, commonly found fish oils. omega-6 (PUFAs; 3 6 PUFAs) essential human health they serve metabolic precursors eicosanoids, class signaling molecules controlling body’s inflammation. Since humans unable produce any or PUFAs, both constitute imperative nutritional ingredients. Long-standing concerns about acids’ impact on management have been supported by experimental investigations significant increases fasting glucose following supplementation foods rich PUFA acid. Cellular explanations explain connection between inflammation IR mitochondrial dysfunction, endoplasmic reticulum (ER) stress, oxidative stress. Modifications lipid composition membranes and/or receptor-mediated may be part mechanism behind activation fusion oil/omega-3 PUFA. The exact molecular processes which PUFAs control activity defend against still unknown.

Language: Английский

Citations

30
Advances in Enantiomer-Dependent Nanotherapeutics DOI
Yuwen Wang, Andy Tay

ACS Nano, Journal Year: 2023, Volume and Issue: 17(11), P. 9850 - 9869

Published: June 2, 2023

The nanoscale properties of nanomaterials, especially nanoparticles, including size, shape, and surface charge, have been extensively studied for their impact on nanomedicine. Given the inherent chiral nature biological systems high enantiomeric selectivity, there is rising interest to manipulate chirality nanomaterials enhance biomolecular interactions improve nanotherapeutics. Chiral nanostructures are currently more prevalently used in biosensing diagnostic applications owing distinctive physical optical properties, but they hold great promise use In this Review, we first discuss stereospecific between biomolecules before comparing synthesis characterization methods nanoparticles nanoassemblies. Finally, examine nanotherapeutics cancer, immunomodulation, neurodegenerative diseases propose plausible mechanisms which interact with cells manipulation. This Review a timely reminder arsenal modifications boost research

Language: Английский

Citations

28
Salidroside alleviates oxidative stress in dry eye disease by activating autophagy through AMPK-Sirt1 pathway DOI Creative Commons

Qi Liang,

Rongjie Guo,

Jia-Ruei Tsao

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 121, P. 110397 - 110397

Published: June 10, 2023

Dry eye disease (DED) is a multifactorial disease, and oxidative stress plays crucial role in its pathogenesis. Recently, multiple studies have shown that upregulation of autophagy can protect the cornea from damage. The present study investigated therapeutic effects salidroside, main component Rhodiola crenulata, both vivo vitro dry models. results showed topical drop treatment with salidroside restored corneal epithelium damage, increased tear secretion, reduced inflammation DED mice. Salidroside activated through AMP-activated protein kinase (AMPK)-sirtuin-1 (Sirt1) signaling pathway, which promoted nuclear translocation factor erythroid-2-related 2 (Nrf2) expression downstream antioxidant factors heme oxygenase-1 (HO-1) NAD(P)H quinone dehydrogenase 1 (NQO1). This process enzyme activity, reactive oxygen species (ROS) accumulation, alleviated stress. application inhibitor chloroquine AMPK Compound C reversed efficacy validating above findings. In conclusion, our data suggest promising candidate for treatment.

Language: Английский

Citations

28
Understanding the molecular mechanism responsible for developing therapeutic radiation-induced radioresistance of rectal cancer and improving the clinical outcomes of radiotherapy - A review DOI Creative Commons

Samatha M. Jain,

Shruthi Nagainallur Ravichandran,

Makalakshmi Murali Kumar

et al.

Cancer Biology & Therapy, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 6, 2024

Rectal cancer accounts for the second highest cancer-related mortality, which is predominant in Western civilizations. The treatment rectal cancers includes surgery, radiotherapy, chemotherapy, and immunotherapy. Radiotherapy, specifically external beam radiation therapy, most common way to treat because not only limits progression but also significantly reduces risk of local recurrence. However, therapeutic radiation-induced radioresistance cells toxicity normal tissues are major drawbacks. Therefore, understanding mechanistic basis developing during after therapy would provide crucial insight improve clinical outcomes patients. Studies by various groups have shown that radiotherapy-mediated changes tumor microenvironment play a role radioresistance. Therapeutic hypoxia functional alterations stromal cells, tumor-associated macrophage (TAM) cancer-associated fibroblasts (CAF), In addition, signaling pathways, such as – PI3K/AKT pathway, Wnt/β-catenin signaling, hippo modulate responsiveness cells. Different radiosensitizers, small molecules, microRNA, nanomaterials, natural chemical sensitizers, being used increase effectiveness radiotherapy. This review highlights mechanism responsible following radiotherapy potential strategies enhance better management cancer.

Language: Английский

Citations

13
Role of oxidative stress‐induced ferroptosis in cancer therapy DOI Creative Commons
Keqing Li, Chengjiang Fan, Jianing Chen

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(10)

Published: May 1, 2024

Abstract Ferroptosis is a distinct mode of cell death, distinguishing itself from typical apoptosis by its reliance on the accumulation iron ions and lipid peroxides. Cells manifest an imbalance between oxidative stress antioxidant equilibrium during certain pathological contexts, such as tumours, resulting in stress. Notably, recent investigations propose that heightened intracellular reactive oxygen species (ROS) due to can heighten cellular susceptibility ferroptosis inducers or expedite onset ferroptosis. Consequently, comprehending role ROS initiation has significance elucidating disorders related Moreover, exhaustive exploration into mechanism control might offer novel targets for addressing specific tumour types. Within this context, our review delves fundamental pathways molecular foundation Four classical ferroptotic are well characterized, namely, glutathione peroxidase 4‐centred pathway, nuclear factor erythroid 2‐related 2 mitochondrial mTOR‐dependent autophagy pathway. Furthermore, we seek elucidate regulatory contributions enacted ROS. Additionally, provide overview targeted medications targeting four implicated their potential clinical applications. Here, ferroptosis, discuss opportunities use new strategy cancer therapy point out current challenges persisting within domain ROS‐regulated anticancer drug research development.

Language: Английский

Citations

12
Fibroblast growth factor 21 alleviates idiopathic pulmonary fibrosis by inhibiting PI3K-AKT-mTOR signaling and stimulating autophagy DOI
Jianying Qi, Yuanyuan Wu, Zhimou Guo

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 273, P. 132896 - 132896

Published: June 7, 2024

Language: Английский

Citations

11