Cotranslational molecular condensation of cochaperones and assembly factors facilitates axonemal dynein biogenesis DOI Creative Commons
Yuanyuan Li, Wenyan Xu, Yubao Cheng

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(47)

Published: Nov. 14, 2024

Axonemal dynein, the macromolecular machine that powers ciliary motility, assembles in cytosol with help of dynein axonemal assembly factors (DNAAFs). These DNAAFs localize cytosolic foci thought to form via liquid-liquid phase separation. However, functional significance DNAAF formation and how production multiple components are so efficiently coordinated, at such enormous scale, remain unclear. Here, we unveil an hub enriched translating heavy chains (HCs) DNAAFs. We show mRNAs encoding interacting HCs outer arms colocalize foci, along nascent HCs. The these mRNA their colocalization relies on HC translation. observe a previously identified assembly, containing Lrrc6 cochaperones Ruvbl1 Ruvbl2, colocalizes is also dependent additionally required for recruitment into both proteins function cotranslationally. propose anchored by stable interactions between HCs, ribosomes, mRNAs, followed cotranslational molecular condensation factors, providing potential mechanism coordinates translation, folding, scale.

Language: Английский

Roles of the CCR4‐Not complex in translation and dynamics of co‐translation events DOI Creative Commons
Martine A. Collart, Léna Audebert, Martin Bushell

et al.

Wiley Interdisciplinary Reviews - RNA, Journal Year: 2023, Volume and Issue: 15(1)

Published: Nov. 27, 2023

Abstract The Ccr4‐Not complex is a global regulator of mRNA metabolism in eukaryotic cells that most well‐known to repress gene expression. Delivery the mRNAs through multitude distinct mechanisms accelerates their decay, yet also plays an important role co‐translational processes, such as association proteins and delivery translating organelles. recent structure Not5 interacting with translated ribosome has brought light embedded information within codon sequence can be monitored by recruitment elongating ribosomes. Thereby, empowered regulatory decisions determining fate being synthesized encoding mRNAs. This review will focus on roles translation dynamics co‐translation events. article categorized under: Translation > Mechanisms Regulation

Language: Английский

Citations

11
Puzzling out nuclear pore complex assembly DOI Creative Commons
Arianna Penzo, Benoı̂t Palancade

FEBS Letters, Journal Year: 2023, Volume and Issue: 597(22), P. 2705 - 2727

Published: Aug. 7, 2023

Nuclear pore complexes (NPCs) are sophisticated multiprotein assemblies embedded within the nuclear envelope and controlling exchanges of molecules between cytoplasm nucleus. In this review, we summarize mechanisms by which these elaborate built from their subunits, nucleoporins, based on our ever-growing knowledge NPC structural organization recent identification additional features process. We present constraints faced during production gathering into oligomeric complexes, formation NPCs envelopes, review cellular strategies at play, co-translational assembly to enrolment a panel cofactors. Remarkably, study can inform perception biogenesis in general - vice versa.

Language: Английский

Citations

8
Orchestrated centers for the production of proteins or “translation factories” DOI Creative Commons
Robert A. Crawford, Matthew Eastham, Martin Pool

et al.

Wiley Interdisciplinary Reviews - RNA, Journal Year: 2024, Volume and Issue: 15(4)

Published: July 1, 2024

The mechanics of how proteins are generated from mRNA is increasingly well understood. However, much less known about protein production coordinated and orchestrated within the crowded intracellular environment, especially in eukaryotic cells. Recent studies suggest that localized sites exist for specific proteins. These have been termed "translation factories" roles complex formation, localization, inheritance, translation regulation postulated. In this article, we review evidence supporting at these sites, details their mechanism likely functional significance. Finally, consider key uncertainties regarding elusive structures This article categorized under: Translation > Mechanisms RNA Export Localization Regulation.

Language: Английский

Citations

1
Hallmarks and evolutionary drivers of cotranslational protein complex assembly DOI Creative Commons
Mihaly Badonyi, Joseph A. Marsh

FEBS Journal, Journal Year: 2023, Volume and Issue: 291(16), P. 3557 - 3567

Published: May 19, 2023

Recent discoveries have highlighted the prevalence of cotranslational assembly in proteomes, revealing a range mechanisms that enables protein complex subunits on ribosome. Structural analyses uncovered emergent properties may inherently control whether subunit undergoes assembly. However, evolutionary paths yielded such complexes over an extended timescale remain largely unclear. In this review, we reflect historical experiments contributed to field, including breakthroughs made possible proteome-wide detection assembly, and technical challenges yet be overcome. We introduce simple framework encapsulates hallmarks discuss how results from new are shaping our view mechanistic, structural factors driving phenomenon.

Language: Английский

Citations

3
Co-translational Assembly Pathways of Nuclear Multiprotein Complexes Involved in the Regulation of Gene Transcription DOI Creative Commons
Andrea Bernardini, Làszlò Tora

Journal of Molecular Biology, Journal Year: 2023, Volume and Issue: 436(4), P. 168382 - 168382

Published: Dec. 5, 2023

Most factors that regulate gene transcription in eukaryotic cells are multimeric, often large, protein complexes. The understanding of the biogenesis pathways such large and heterogeneous assemblies, as well dimerization partner choice among factors, is crucial to interpret control expression programs consequent cell fate decisions. Co-translational assembly (Co-TA) thought play key roles complexes by directing complex formation during synthesis. In this review we discuss principles Co-TA with a special focus for regulatory We outline expected molecular advantages establishing co-translational interactions, pointing at available, or missing, evidence each them. hypothesize different mechanisms based on explain allocation "dilemma" paralog proteins subunits shared By taking paradigm employed three related (TFIID, SAGA ATAC), alternative strategies nuclear multiprotein widespread – yet specific use involved transcription. Ultimately, outlined series open questions which demand well-defined lines research investigate regulation rely coordinated

Language: Английский

Citations

2
Cotranslational molecular condensation of cochaperones and assembly factors facilitates axonemal dynein biogenesis DOI Creative Commons
Yuanyuan Li, Wenyan Xu, Yubao Cheng

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(47)

Published: Nov. 14, 2024

Axonemal dynein, the macromolecular machine that powers ciliary motility, assembles in cytosol with help of dynein axonemal assembly factors (DNAAFs). These DNAAFs localize cytosolic foci thought to form via liquid-liquid phase separation. However, functional significance DNAAF formation and how production multiple components are so efficiently coordinated, at such enormous scale, remain unclear. Here, we unveil an hub enriched translating heavy chains (HCs) DNAAFs. We show mRNAs encoding interacting HCs outer arms colocalize foci, along nascent HCs. The these mRNA their colocalization relies on HC translation. observe a previously identified assembly, containing Lrrc6 cochaperones Ruvbl1 Ruvbl2, colocalizes is also dependent additionally required for recruitment into both proteins function cotranslationally. propose anchored by stable interactions between HCs, ribosomes, mRNAs, followed cotranslational molecular condensation factors, providing potential mechanism coordinates translation, folding, scale.

Language: Английский

Citations

0