Cre-Dependent Anterograde Transsynaptic Labeling and Functional Imaging in Zebrafish Using VSV With Reduced Cytotoxicity DOI Creative Commons

Stanislav Kler,

Manxiu Ma, Sujatha Narayan

et al.

Frontiers in Neuroanatomy, Journal Year: 2021, Volume and Issue: 15

Published: Oct. 6, 2021

The small size and translucency of larval zebrafish ( Danio rerio ) have made it a unique experimental system to investigate whole-brain neural circuit structure function. Still, the connectivity patterns between most neuronal types remain mostly unknown. This gap in knowledge underscores critical need for effective mapping tools, especially ones that can integrate structural functional analyses. To address this, we previously developed vesicular stomatitis virus (VSV) based approach called Tracer with Restricted Anterograde Spread (TRAS). TRAS utilizes lentivirus complement replication-incompetent VSV (VSVΔG) allow restricted (monosynaptic) anterograde labeling from projection neurons their target cells brain. Here, report second generation (TRAS-M51R), which mutant variant VSVΔG [VSV(M51R)ΔG] reduced cytotoxicity. Within primary visual pathway, found TRAS-M51R significantly improved long-term viability transsynaptic (compared TRAS) while maintaining spread activity. By using Cre-expressing VSV(M51R)ΔG, could selectively label excitatory vglut2a positive) inhibitory gad1b retinorecipient neurons. We further show these labeled retained excitability upon stimulation at 5–8 days post fertilization (2–5 post-infection). Together, findings is suitable studies connectivity, cell-type identity, neurophysiology.

Language: Английский

Automated synapse-level reconstruction of neural circuits in the larval zebrafish brain DOI Creative Commons

Fabian Svara,

Dominique Förster, Fumi Kubo

et al.

Nature Methods, Journal Year: 2022, Volume and Issue: 19(11), P. 1357 - 1366

Published: Oct. 24, 2022

Abstract Dense reconstruction of synaptic connectivity requires high-resolution electron microscopy images entire brains and tools to efficiently trace neuronal wires across the volume. To generate such a resource, we sectioned imaged larval zebrafish brain by serial block-face at voxel size 14 × 25 nm 3 . We segmented resulting dataset with flood-filling network algorithm, automated detection chemical synapses validated results comparisons transmission microscopic light-microscopic reconstructions. Neurons their connections are stored in form queryable expandable digital address book. reconstructed 208 neurons involved visual motion processing, most them located pretectum, which had been functionally characterized same specimen two-photon calcium imaging. Moreover, mapped all 407 presynaptic postsynaptic partners two superficial interneurons tectum. The resource developed here serves as foundation for synaptic-resolution circuit analyses nervous system.

Language: Английский

Citations

75
Visual recognition of social signals by a tectothalamic neural circuit DOI Creative Commons
Johannes M. Kappel, Dominique Förster, Katja Slangewal

et al.

Nature, Journal Year: 2022, Volume and Issue: 608(7921), P. 146 - 152

Published: July 13, 2022

Abstract Social affiliation emerges from individual-level behavioural rules that are driven by conspecific signals 1–5 . Long-distance attraction and short-distance repulsion, for example, jointly set a preferred interanimal distance in swarms 6–8 However, little is known about their perceptual mechanisms executive neural circuits 3 Here we trace the neuronal response to self-like biological motion 9,10 , visual trigger developing zebrafish 2,11 Unbiased activity mapping targeted volumetric two-photon calcium imaging revealed 21 hotspots distributed throughout brain as well clustered biological-motion-tuned neurons multimodal, socially activated nucleus of dorsal thalamus. Individual thalamus encode local acceleration stimuli mimicking typical fish kinetics but insensitive global or continuous motion. Electron microscopic reconstruction synaptic input optic tectum projections into hypothalamic areas with conserved social function 12–14 Ablation selectively disrupted without affecting repulsion. This tectothalamic pathway thus serves recognition conspecifics, dissociates control repulsion during affiliation, revealing circuit underpinning collective behaviour.

Language: Английский

Citations

59
Myelination-independent functions of oligodendrocyte precursor cells in health and disease DOI
Yan Xiao, Tim Czopka

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 26(10), P. 1663 - 1669

Published: Aug. 31, 2023

Language: Английский

Citations

34
The Visual Systems of Zebrafish DOI
Herwig Baier, Ethan K. Scott

Annual Review of Neuroscience, Journal Year: 2024, Volume and Issue: 47(1), P. 255 - 276

Published: April 25, 2024

The zebrafish visual system has become a paradigmatic preparation for behavioral and systems neuroscience. Around 40 types of retinal ganglion cells (RGCs) serve as matched filters stimulus features, including light, optic flow, prey, objects on collision course. RGCs distribute their signals via axon collaterals to 12 retinorecipient areas in forebrain midbrain. major visuomotor hub, the tectum, harbors nine RGC input layers that combine information multiple features. retinotopic map tectum is locally adapted scene statistics subfield–specific demands. Tectal projections premotor centers are topographically organized according commands. known connectivity more than 20 processing streams allows us dissect cellular basis elementary perceptual cognitive functions. Visually evoked responses, such prey capture or loom avoidance, controlled by dedicated multistation pathways that—at least larva—resemble labeled lines. This architecture serves neuronal code's purpose driving adaptive behavior.

Language: Английский

Citations

9
From perception to behavior: The neural circuits underlying prey hunting in larval zebrafish DOI Creative Commons
Shuyu Zhu, Geoffrey J. Goodhill

Frontiers in Neural Circuits, Journal Year: 2023, Volume and Issue: 17

Published: Feb. 1, 2023

A key challenge for neural systems is to extract relevant information from the environment and make appropriate behavioral responses. The larval zebrafish offers an exciting opportunity studying these sensing processes sensory-motor transformations. Prey hunting instinctual behavior of that requires brain combine different attributes sensory input form motor outputs. Due its small size transparency allows optical recording whole-brain activity reveal mechanisms involved in prey capture. In this review we discuss how visual identify locate prey, circuits governing generation commands response can be modulated by internal states experience, some outstanding questions field.

Language: Английский

Citations

18
Real-time evaluation of glioblastoma growth in patient-specific zebrafish xenografts DOI Creative Commons
Elin Almstedt, Emil Rosén, Marleen Gloger

et al.

Neuro-Oncology, Journal Year: 2021, Volume and Issue: 24(5), P. 726 - 738

Published: Nov. 17, 2021

Abstract Background Patient-derived xenograft (PDX) models of glioblastoma (GBM) are a central tool for neuro-oncology research and drug development, enabling the detection patient-specific differences in growth, vivo response. However, existing PDX not well suited large-scale or automated studies. Thus, here, we investigate if fast zebrafish-based model, supported by longitudinal, AI-driven image analysis, can recapitulate key aspects growth enable case-comparative testing. Methods We engrafted 11 GFP-tagged patient-derived GBM IDH wild-type cell cultures (PDCs) into 1-day-old zebrafish embryos, monitored fish with 96-well live microscopy convolutional neural network analysis. Using light-sheet imaging whole analyzed further invasive tumor cells. Results Our pipeline enables automatic robust longitudinal observation survival individual fish. The PDCs expressed invasion heterogeneity, initiation correlated strongly matched mouse counterparts (Spearman R = 0.89, p < 0.001). Three showed high degree association between grafted cells host blood vessels, suggesting perivascular phenotype. In evaluation marizomib, currently clinical trials GBM, an effect on corresponding to PDC vitro marizomib sensitivity. Conclusions Zebrafish xenografts AI methods process, present scalable alternative study initiation, invasion, applicable evaluation.

Language: Английский

Citations

31
Neural Circuit Mechanisms Involved in Animals’ Detection of and Response to Visual Threats DOI
Qiwen Wu, Yifeng Zhang

Neuroscience Bulletin, Journal Year: 2023, Volume and Issue: 39(6), P. 994 - 1008

Published: Jan. 25, 2023

Language: Английский

Citations

11
Pannexin-2 deficiency disrupts visual pathways and leads to ocular defects in zebrafish DOI
Riya Shanbhag, Georg Zoidl,

Fatema Nakhuda

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2025, Volume and Issue: unknown, P. 167807 - 167807

Published: March 1, 2025

Language: Английский

Citations

0
Object identity representation occurs early in the archerfish visual system DOI Creative Commons
Svetlana Volotsky, Ronen Segev

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 3, 2025

Language: Английский

Citations

0
Cardiac interoception impacts behavior and brain-wide neuronal dynamics DOI Creative Commons
Kristian J. Herrera,

Arman Zarghani-Shiraz,

Misha B. Ahrens

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

SUMMARY We sought to explore the question as whether an animal’s behavior can be modified by internal physiological changes. focused on optomotor response, in which animal moves response visual gratings, because it is quantitative, robust, and evolutionarily conserved. Using larval zebrafish, we demonstrate that engagement inversely related heart rate. modulate rate external threat, activation or blockade of sympathetic nervous system, pharmacological cardiac pacemaker channel, direct optogenetic pacing heart, find correlation persists through all perturbations. neurons primary sensory ganglia several regions brain whose activity reflects changes rate, some more active during bradycardia tachycardia. Specifically, show area postrema, known a center cardiovascular integration, shows particularly strong encoding both following threat pacing. suggest there may neural mechanisms assess over time, this interoceptive measurement used regulate other circuits behavioral output.

Language: Английский

Citations

0