Viscerosensory signalling to the nucleus accumbens via the solitary tract nucleus DOI Creative Commons
Stuart J. McDougall, Zhi Yi Ong,

Rosa Heller

et al.

Journal of Neurochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: July 20, 2024

Abstract The nucleus of the solitary tract (NTS) receives direct viscerosensory vagal afferent input that drives autonomic reflexes, neuroendocrine function and modulates behaviour. A subpopulation NTS neurons project to accumbens (NAc); however, this NTS‐NAc pathway remains unknown. combination neuroanatomical tracing, slice electrophysiology fibre photometry was used in mice and/or rats determine how fit within network. projection are predominantly located medial caudal portions with 54 ± 7% (mice) 65 3% (rat) being TH‐positive, representing A2 cell group. In horizontal brainstem slices, (ST) stimulation evoked excitatory post‐synaptic currents (EPSCs) neurons. majority (75%) received low‐jitter, zero‐failure EPSCs characteristic monosynaptic ST identifies them as second order primary sensory We then examined whether respond cholecystokinin (CCK, 20 μg/kg ip) vivo both rats. Surprisingly, there no difference number activated cells between CCK saline‐treated mice. rats, just 6% were recruited by CCK. As TH source for NAc noradrenaline, we measured noradrenaline release showed levels declined response cue‐induced reward retrieval but not foot shock. Combined, these findings suggest high‐fidelity information from afferents reaches NAc. These signals likely unrelated CCK‐sensitive could interact other higher inputs modulate learned appetitive behaviours. image

Language: Английский

Vagotomy blunts cardiorespiratory responses to vagal afferent stimulation via pre‐ and postsynaptic effects in the nucleus tractus solitarii DOI
Gabrielle C. Hofmann, Procópio Cleber Gama de Barcellos Filho,

Fateme Khodadadi

et al.

The Journal of Physiology, Journal Year: 2024, Volume and Issue: 602(6), P. 1147 - 1174

Published: Feb. 20, 2024

Abstract Viscerosensory information travels to the brain via vagal afferents, where it is first integrated within brainstem nucleus tractus solitarii (nTS), a critical contributor cardiorespiratory function and site of neuroplasticity. We have shown that decreasing input nTS unilateral vagus nerve transection (vagotomy) induces morphological changes in glia reduces sighs during hypoxia. The mechanisms behind post‐vagotomy are not well understood. hypothesized chronic vagotomy alters responses afferent stimulation blunted neuronal activity. Male Sprague–Dawley rats (6 weeks old) underwent right cervical caudal nodose ganglion, or sham surgery. After 1 week, were anaesthetized, ventilated instrumented measure mean arterial pressure (MAP), heart rate (HR), splanchnic sympathetic phrenic activity (SSNA PhrNA, respectively). Vagal (2–50 Hz) decreased parameters increased Ca 2+ measured by vivo photometry vitro slice imaging GCaMP8m. Vagotomy attenuated both these reflex compared shams. also reduced presynaptic (Cal‐520 imaging) slice. decrease HR, SSNA PhrNA due nanoinjection exogenous glutamate was tempered following vagotomy. This effect restored blocking excitatory amino acid transporters. However, mimicked NMDA, AMPA, associated with NR1 subunits nTS. Altogether, results demonstrate multiple tripartite synapse influence stimulation. image Key points Multiple (nTS) contribute functional transection. nanoinjection. Blunted occur activation NMDA receptor expression function, leading reduction activation. These provide insight into control autonomic respiratory as plasticity can response damage disease.

Language: Английский

Citations

2
Differential transcriptional profiles of vagal sensory neurons in female and male mice DOI Creative Commons
Young‐Hwan Jo

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: May 14, 2024

Introduction Differences in metabolic homeostasis, diabetes, and obesity between males females are evident rodents humans. Vagal sensory neurons the vagus nerve ganglia innervate a variety of visceral organs use specialized endings to sense interoceptive signals. This organ-brain axis plays role relaying signals higher brain centers, as well regulating vago-vagal reflex. I hypothesized that molecularly distinct populations vagal would play causing differences homeostasis sexes. Methods SnRNA-Seq was conducted on dissociated cells from using 10X Genomics Chromium platform. Results Single-nucleus RNA sequencing analysis female male mice revealed transcriptional profiles ganglia. These linked expression sex-specific genes such Xist , Tsix Ddx3y . Among 13 neuronal clusters, one-fourth were located -enriched VN1 VN8 which displayed enrichment Trpv1 Piezo2 Htr3a Vip genes. In contrast, 70% found clusters VN4, 6, 7, 10, 11, 13, showed enriched Fgfr1 Lpar1 Cpe Esr1 Nrg1 Egfr Oprm1 Two satellite identified, one contained oligodendrocyte precursor mice. A small population expressed Ucp1 Plin1 indicating they epineural adipocytes. Discussion Understanding physiological implications transcriptomic energy balance help develop treatments for dysregulation.

Language: Английский

Citations

2
Gut–vagus–NTS neural pathway in controlling feeding behaviors DOI Creative Commons
Jing Chen, Cheng Zhan

Stress and Brain, Journal Year: 2023, Volume and Issue: 3(1), P. 19 - 34

Published: March 1, 2023

Obesity has become a worldwide disease, posing rapidly increasing challenge to the global healthcare system. The primary reason for obesity is that food intake exceeds body's needs. central nervous system monitors energy status by continuously receiving peripheral gut-derived signals and functions as master regulator in controlling feeding behaviors. Vagal afferents transmit consumption from periphery hindbrain (e.g., nucleus of solitary tract (NTS)). In contrast, vagal efferent nerves send commands regulate organ activities. However, precise role gut–vagus–NTS pathway gut–brain axis regulating not yet fully understood. This review highlights key roles NTS, sensory neurons, gastrointestinal

Language: Английский

Citations

4
Central amygdala is related to the reduction of aggressive behavior by monosodium glutamate ingestion during the period of development in an ADHD model rat DOI Creative Commons

Dewi Mustika,

Yu Nishimura,

Shinya Ueno

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: May 3, 2024

Introduction Monosodium glutamate (MSG), an umami substance, stimulates the gut-brain axis communication via gut receptors and subsequent vagus nerves. However, brain mechanism underlying effect of MSG ingestion during developmental period on aggression has not yet been clarified. We first tried to establish new experimental conditions be more appropriate for detailed analysis brain, then investigated effects aggressive behavior stage ADHD rat model. Methods Long-Evans, WKY/Izm, SHR/Izm, SHR-SP/Ezo were individually housed from postnatal day 25 5 weeks. Post-weaning social isolation (PWSI) was given escalate behavior. The resident-intruder test, that is conducted subjective night, used a aggression, including frequency, duration, latency anogenital sniffing, grooming, attack Immunohistochemistry c-Fos expression in all strains predict potential aggression-related areas. Finally, most strain, known model attention-deficit hyperactivity disorder (ADHD), investigate (60 mM solution) followed by immunostaining Bilateral subdiaphragmatic vagotomy performed verify importance interactions MSG. Results resident intruder test revealed SHR/Izm rats among four parameters tested. also showed highest number + cells areas, central amygdala (CeA). significantly decreased frequency duration grooming increased Furthermore, administration successfully positive cell intermediate nucleus solitary tract (iNTS), terminal gastrointestinal sensory afferent fiber nerve, modulated CeA. Interestingly, diminished iNTS Conclusion PWSI-induced which mediated nerve related stimulation modulation CeA activity.

Language: Английский

Citations

1
Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice DOI Creative Commons

Md Sams Sazzad Ali,

Ghazaal Parastooei,

Swarnalakshmi Raman

et al.

Brain stimulation, Journal Year: 2024, Volume and Issue: 17(5), P. 987 - 1000

Published: Aug. 23, 2024

Vagus nerve stimulation (VNS) is clinically useful for treating epilepsy, depression, and chronic pain. Currently, cervical VNS (cVNS) treatment well-established, while auricular (aVNS) under development. Vagal regulates functions in diverse brain regions; therefore, it critical to better understand how electrically-evoked vagal inputs following cVNS aVNS engage with different regions.

Language: Английский

Citations

1
Molecular Ontology of the Nucleus of Solitary Tract DOI Creative Commons
Silvia Gasparini, Gislaine Almeida‐Pereira,

Ana Sofia Peraza Munuzuri

et al.

The Journal of Comparative Neurology, Journal Year: 2024, Volume and Issue: 532(12)

Published: Dec. 1, 2024

ABSTRACT The nucleus of the solitary tract (NTS) receives visceral information and regulates appetitive, digestive, cardiorespiratory systems. Within NTS, diverse processes operate in parallel to sustain life, but our understanding their cellular composition is incomplete. Here, we integrate histologic transcriptomic analysis identify compare molecular features that distinguish neurons this brain region. Most glutamatergic NTS area postrema co‐express transcription factors Lmx1b Phox2b , except for a ventral band far‐caudal which include Gcg ‐expressing produce glucagon‐like peptide 1 (GLP‐1). GABAergic interneurons intermingle through + macropopulation, dense clusters surround NTS. macropopulation includes subpopulations with distinct distributions expressing Grp Hsd11b2, Npff Pdyn Pou3f1 Sctr Th other markers. These findings highlight – co‐expression as common feature improve organization distribution critical

Language: Английский

Citations

1
Selective transduction and photoinhibition of pre-Bötzinger complex neurons that project to the facial nucleus in rats affects nasofacial activity DOI Creative Commons
Mariana Melo, Alexander D. Wykes, Angela A. Connelly

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Sept. 29, 2023

The pre-Bötzinger complex (preBötC), a key primary generator of the inspiratory breathing rhythm, contains neurons that project directly to facial nucleus (7n) motoneurons coordinate orofacial and nasofacial activity. To further understand identity 7n-projecting preBötC neurons, we used combination optogenetic viral transgenic approaches demonstrate selective photoinhibition these affects mystacial pad activity, with minimal effects on breathing. These are altered by type anesthetic employed also between anesthetized conscious states. population transduced consisted both excitatory inhibitory send collaterals multiple brainstem nuclei involved regulation autonomic We show modulation subgroups based their axonal projections, is useful strategy improve our understanding mechanisms integrate different motor physiological behaviors. This fundamental importance, given abnormal respiratory activity behaviors have been associated development progression diseases.

Language: Английский

Citations

3
Comparative localization of colorectal sensory afferent central projections in the mouse spinal cord dorsal horn and caudal medulla dorsal vagal complex DOI Creative Commons
Qingqing Wang, Sonia Garcia‐Caraballo, Grigori Y. Rychkov

et al.

The Journal of Comparative Neurology, Journal Year: 2023, Volume and Issue: 532(2)

Published: Oct. 14, 2023

Abstract The distal colon and rectum (colorectum) are innervated by spinal vagal afferent pathways. central circuits into which afferents relay colorectal nociceptive information remain to be comparatively assessed. To address this, regional retrograde tracing distension (CRD)‐evoked neuronal activation were used compare the within dorsal complex (DVC) horn (thoracolumbar [TL] lumbosacral [LS] levels) project. Vagal projections observed in nucleus tractus solitarius (NTS), area postrema (AP), motor of vagus (DMV), labeled from rostral colorectum. In NTS, opposed catecholamine pontine parabrachial nuclei (PbN)‐projecting neurons. Spinal through caudal aspects horn, number neurons activated CRD was linked pressure intensity, unlike DVC. 13% ± 0.6% CRD‐activated projected PbN. at TL level, input associated with PbN‐projecting lamina I (LI), 63% 3.15% LI projecting On other hand, LS only 18% collective data identify differences neuroanatomy that support disparate roles signaling facilitation modulation responses.

Language: Английский

Citations

3
Control of sodium appetite by hindbrain aldosterone-sensitive neurons DOI

Ahmet Kuralay,

Miriam C. McDonough, Jon M. Resch

et al.

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 592, P. 112323 - 112323

Published: June 26, 2024

Language: Английский

Citations

0
A Simple and Fast Optical Clearing Method for Whole‐Mount Fluorescence In Situ Hybridization (FISH) Imaging DOI Creative Commons
Junwoo Suh, Yehe Liu,

Jordan Smith

et al.

Journal of Biophotonics, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

We report a single-step optical clearing method that is compatible with RNA fluorescence in situ hybridization (FISH) imaging. previously demonstrated microscopy imaging immunohistochemistry and genetic reporters using technique called lipid-preserving refractive index matching for prolonged depth (LIMPID). Our protocol reliably produces high-resolution three-dimensional (3D) images minimal aberrations high magnification objectives, captures large field-of-view of whole-mount tissues, supports co-labeling antibody FISH probes. also custom-designed probes quail embryos, demonstrating the ease fabricating use less common animal models. Furthermore, we show high-quality 3D conventional microscope, without more advanced sectioning instruments such as confocal or light-sheet microscopy. For broader adoption, simplified optimized 3D-LIMPID-FISH to minimize barrier entry, provide detailed aid users navigating thick thin

Language: Английский

Citations

0