Monitoring of biologically active substances in clinical samples by capillary and microchip electrophoresis with contactless conductivity detection: A review

Analytica Chimica Acta, Journal Year: 2022, Volume and Issue: 1225, P. 340161 - 340161

Published: July 18, 2022

Language: Английский

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Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

Electrophoresis, Journal Year: 2023, Volume and Issue: 44(17-18), P. 1279 - 1341

Published: Aug. 3, 2023

Abstract This review is in support of the development selective, precise, fast, and validated capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H, Degenhardt M, Kunkel A. “Strategies for electrophoresis: method validation pharmaceutical biological applications,” pointing out which fundamentals are still valid at same time showing enormous achievements last 25 years. The structures both reviews widely similar, order to facilitate their simultaneous use. Focusing on applications, successful use CE now demonstrated by more than 600 carefully selected references. Many those recent reviews; therefore, significant overview about field provided. There extra sections sample pretreatment related microchip CE, completely revised section protein analytes biomolecules general. general strategies summed with regard selectivity, efficiency, precision, analysis time, limit detection, requirements, validation.

Language: Английский

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Progress in on-line, at-line, and in-line coupling of sample treatment with capillary and microchip electrophoresis over the past 10 years: A review

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1261, P. 341249 - 341249

Published: April 22, 2023

Language: Английский

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Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Journal of Separation Science, Journal Year: 2023, Volume and Issue: 46(15)

Published: May 26, 2023

Capillary electrophoresis is recognized as a valued separation technique for its high efficiency, low sample consumption, good economic and ecological aspects, reproducibility, complementarity to traditional liquid chromatography techniques. experiments are generally performed utilizing optical detection, such ultraviolet or fluorescence detectors. However, in order provide structural information, capillary hyphenated highly sensitive selective mass spectrometry has been developed overcome the limitations of detections. electrophoresis‐mass increasingly popular protein analysis, including biopharmaceutical biomedical research. It frequently applied determination physicochemical biochemical parameters proteins, offers excellent performance in‐depth characterizations biopharmaceuticals at various levels also already proven promising tool biomarker discovery. In this review, we focus on possibilities analysis their intact level. Various modes interfaces, well approaches prevent adsorption enhance loading capacity, discussed recent (2018–March 2023) developments applications field summarized.

Language: Английский

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Performance of capillary electrophoresis instruments – State of the art and outlook

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 163, P. 117056 - 117056

Published: April 13, 2023

Language: Английский

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Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (mid‐2018–2022)

Journal of Separation Science, Journal Year: 2023, Volume and Issue: 46(12)

Published: Feb. 26, 2023

This review gives a wide overview of recent advances and applications capillary electrophoresis microchip methods in the fields proteomics peptidomics period from mid‐2018 up to end 2022. The methodological topics covering sample preparation concentration techniques, hyphenation with mass spectrometry, multidimensional separations by on‐line or off‐line coupled different liquid chromatography techniques are described new developments both bottom‐up top‐down approaches presented. In addition, various proteomic peptidomic studies demonstrated. They include monitoring protein posttranslational modifications biological biochemical research, clinical proteomics, food analysis.

Language: Английский

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Molecular Separation by Using Active and Passive Microfluidic chip Designs: A Comprehensive Review

Advanced Materials Interfaces, Journal Year: 2023, Volume and Issue: 11(2)

Published: Oct. 27, 2023

Abstract Separation and identification of molecules biomolecules such as nucleic acids, proteins, polysaccharides from complex fluids are known to be important due unmet needs in various applications. Generally, many different separation techniques, including chromatography, electrophoresis, magnetophoresis, have been developed identify the target precisely. However, these techniques expensive time consuming. “Lab‐on‐a‐chip” systems with low cost per device, quick analysis capabilities, minimal sample consumption seem ideal candidates for separating particles, cells, blood samples, molecules. From this perspective, microfluidic‐based extensively past two decades separate samples origins. In review, “lab‐on‐a‐chip” methods by passive, active, hybrid approaches decade comprehensively discussed. Due wide variety field, it will impossible cover every facet subject. Therefore, review paper covers passive active generally used biomolecule separation. Then, an investigation combined sophisticated is highlighted. The spotlight also shined on elegance successes recent years, remainder article explores how permit development novel techniques.

Language: Английский

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Food Peptides for the Nutricosmetic Industry

Antioxidants, Journal Year: 2023, Volume and Issue: 12(4), P. 788 - 788

Published: March 23, 2023

In recent years, numerous reports have described bioactive peptides (biopeptides)/hydrolysates produced from various food sources. Biopeptides are considered interesting for industrial application since they show functional properties (e.g., anti-aging, antioxidant, anti-inflammatory, and antimicrobial properties) technological solubility, emulsifying, foaming). Moreover, fewer side effects than synthetic drugs. Nevertheless, some challenges must be overcome before their administration via the oral route. The gastric, pancreatic, small intestinal enzymes acidic stomach conditions can affect bioavailability levels that reach site of action. Some delivery systems been studied to avoid these problems microemulsions, liposomes, solid lipid particles). This paper summarizes results studies conducted on biopeptides isolated plants, marine organisms, animals, biowaste by-products, discusses potential in nutricosmetic industry, considers could maintain bioactivity. Our environmentally sustainable products used as antimicrobial, anti-inflammatory agents formulations. Biopeptide production requires expertise analytical procedures good manufacturing practice. It is hoped new developed simplify large-scale authorities adopt regulate use appropriate testing standards guarantee population’s safety.

Language: Английский

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Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Electrophoresis, Journal Year: 2023, Volume and Issue: 45(1-2), P. 165 - 198

Published: Sept. 5, 2023

Abstract This review article brings a comprehensive survey of developments and applications high‐performance capillary microchip electromigration methods (zone electrophoresis in free solution or sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, electrochromatography) for analysis, micropreparation, physicochemical characterization peptides the period from 2021 up to ca. middle 2023. Progress study properties various aspects their such as sample preparation, adsorption suppression, electroosmotic flow regulation, detection, are presented. New particular demonstrated, several types reported. They cover qualitative quantitative analysis synthetic isolated determination complex biomatrices, peptide profiling biofluids tissues, monitoring chemical enzymatic reactions changes peptides. include also amino acid sequence peptides, mapping proteins, separation stereoisomers chiral analyses. In addition, micropreparative separations interactions with other (bio)molecules by above CE described.

Language: Английский

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Peptide mapping of proteins by capillary electromigration methods

Journal of Separation Science, Journal Year: 2022, Volume and Issue: 45(23), P. 4245 - 4279

Published: Oct. 6, 2022

This review article provides a wide overview of important developments and applications capillary electromigration methods in the area peptide mapping proteins period 1997-mid-2022, including articles on this topic. It deals with all major aspects by methods: i) precleavage sample preparation involving purification, preconcentration, denaturation, reduction alkylation protein(s) to be analyzed, ii) generation fragments off-line or on-line enzymatic and/or chemical cleavage protein(s), iii) postcleavage generated mixture for separation, iv) separation complex mixtures one-, two- multidimensional coupled mass spectrometry detection, v) large application variable purposes, such as qualitative analysis monoclonal antibodies other protein biopharmaceuticals, monitoring posttranslational modifications, determination primary structure investigation function biochemical clinical research, characterization origin well identification proteomic peptidomic studies.

Language: Английский

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