A capillary-based microfluidic chip with the merits of low cost and easy fabrication for the rapid detection of acute myocardial infarction

Talanta, Journal Year: 2023, Volume and Issue: 265, P. 124924 - 124924

Published: July 7, 2023

Language: Английский

---

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Electrophoresis, Journal Year: 2023, Volume and Issue: 45(1-2), P. 165 - 198

Published: Sept. 5, 2023

Abstract This review article brings a comprehensive survey of developments and applications high‐performance capillary microchip electromigration methods (zone electrophoresis in free solution or sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, electrochromatography) for analysis, micropreparation, physicochemical characterization peptides the period from 2021 up to ca. middle 2023. Progress study properties various aspects their such as sample preparation, adsorption suppression, electroosmotic flow regulation, detection, are presented. New particular demonstrated, several types reported. They cover qualitative quantitative analysis synthetic isolated determination complex biomatrices, peptide profiling biofluids tissues, monitoring chemical enzymatic reactions changes peptides. include also amino acid sequence peptides, mapping proteins, separation stereoisomers chiral analyses. In addition, micropreparative separations interactions with other (bio)molecules by above CE described.

Language: Английский

---

Recent advances in microextraction techniques using sustainable green solvents for mass spectrometry analysis

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 170, P. 117412 - 117412

Published: Nov. 7, 2023

Language: Английский

---

Chiral Analysis of Pesticides and Emerging Contaminants by Capillary Electrophoresis—Application to Toxicity Evaluation

Toxics, Journal Year: 2024, Volume and Issue: 12(3), P. 185 - 185

Published: Feb. 28, 2024

Chiral analysis is a very relevant topic in environmental chemistry. This due to the different properties of stereoisomers chiral compounds. In case agrochemicals, desired activity, degradation rate, or toxicity, among other characteristics, may differ between stereoisomers, and same true for emerging contaminants, such as pharmaceuticals cosmetics. Therefore, development analytical methodologies enabling their determination samples interest paramount. Although techniques have been widely employed carry out separations, HPLC, GC, SFC, capillary electrophoresis (CE) has attracted lot attention field its simplicity, flexibility, low cost. fact, chromatographic columns are not needed, consumption reagents small dimensions separation capillaries. article reviews characteristics developed by CE stereoselective pesticides contaminants (water soil), well food commercial agrochemical formulations. Applications stability toxicity studies these also reviewed.

Language: Английский

---

Phenolic compounds determination in stingless bee by dispersive liquid-liquid microextraction coupled with capillary electrophoresis − diode array detection

Food Chemistry, Journal Year: 2025, Volume and Issue: 481, P. 143720 - 143720

Published: March 5, 2025

Language: Английский

---

Advances in capillary electrophoresis for plant analysis

Talanta, Journal Year: 2025, Volume and Issue: 293, P. 128171 - 128171

Published: April 17, 2025

Language: Английский

---

Breaking Barriers: Exploring Neurotransmitters through In Vivo vs. In Vitro Rivalry

Sensors, Journal Year: 2024, Volume and Issue: 24(2), P. 647 - 647

Published: Jan. 19, 2024

Neurotransmitter analysis plays a pivotal role in diagnosing and managing neurodegenerative diseases, often characterized by disturbances neurotransmitter systems. However, prevailing methods for quantifying neurotransmitters involve invasive procedures or require bulky imaging equipment, therefore restricting accessibility posing potential risks to patients. The innovation of compact, vivo instruments neurotransmission holds the reshape disease management. This can facilitate non-invasive uninterrupted monitoring levels their activity. Recent strides microfabrication have led emergence diminutive that also find applicability vitro investigations. By harnessing synergistic microfluidics, micro-optics, microelectronics, this nascent realm research substantial promise. review offers an overarching view current sensing techniques, advances towards microsensors tailored neurotransmission, state-of-the-art fabrication techniques be used fabricate those microsensors.

Language: Английский

---

On-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry for the analysis of protein biomarkers in biological fluids and food: A tutorial

Advances in Sample Preparation, Journal Year: 2023, Volume and Issue: 7, P. 100082 - 100082

Published: July 4, 2023

The analysis by capillary electrophoresis (CE) of low abundant proteins in complex samples, such as biological fluids and food, is especially challenging, due to the poor concentration sensitivity microscale separation techniques sample matrix complexity. In order overcome these major drawbacks, microextraction preparation based on on-line solid-phase extraction (SPE-CE) are regarded an excellent alternative for clean-up analyte preconcentration with minimum handling. this study, we present, a tutorial, valve-free aptamer affinity electrophoresis-mass spectrometry (AA-SPE-CE-MS) method purification, preconcentration, separation, detection, characterization intact protein biomarkers food using cases study α‑synuclein (α‑syn), concanavalin A (Con A), β-lactoglobulin (β-LG), which related Parkinson's disease allergy, respectively. This tutorial not limited description analytical method, but it also provides ready-to-use procedures sorbent devices, introduces strategies undesired effects, allowing straightforward implementation optimum performance AA-SPE-CE-MS, platform develop further applications.

Language: Английский

---

Greenness of proteomic sample preparation and analysis techniques for biopharmaceuticals

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 171, P. 117490 - 117490

Published: Dec. 19, 2023

Proteins are efficient therapeutic agents and attractive potential new biomarkers due to their physiological pathological functions. Further, biopharmaceuticals feature complex protein architectures that must be assayed quality controlled. Capillary electrophoresis (CE) has become one of the most powerful tools for proteomic analysis. CE is an eco-friendlier alternative traditional liquid chromatography; however, its true greenness environmental impact not very often confirmed by green metrics. Here, we assessed bottom-up, middle-up, top-down sample preparation analytical approaches analysis proteins using AGREEprep AGREE metric systems, respectively. The combined use both can help identify weaknesses overall procedure point toward greener alternatives. results showed trends in workflows should focused mainly on miniaturization devices applying in-line on-line preparation.

Language: Английский

---

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods

International Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 4(2), P. 309 - 333

Published: May 31, 2024

Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage formation, these disorders effectively treated, leading to successful outcomes; curative therapies halt illnesses from advancing, thus improving quality life and long-term survival patient. Still, cutting-edge upgrades precision technologies necessary for early, reliable, affordable, rapid disease detection, but also vital well-being people future global public health. The emerging role utility non-invasive repeatable diagnostic test approaches detection health conditions have been exemplified by liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator signature (e.g., proteins) provide valuable information an individual’s wellness and/or Among bodily secretions used diagnostics is sputum, complex viscous gel-like biopolymeric network gained growing recognition as rich source biomarkers airway infections pulmonary diseases, serves determinant reveal other illnesses. per World Health Organization, burden respiratory exacerbated factors ranging considerable subjection air pollution occupational contaminants tobacco smoking second-hand smoke, addition poor socio-economic status. Due likely increase determinants, tract rise, affecting many individuals, putting stress healthcare facilities services worldwide. aim this study was perform narrative review sputum constituents with emphasis proteins glycoproteins assessed possible lung organ diseases. A search conducted using mucus, proteomics, biomarkers, point-of-care testing keywords employing Google, PubMed (MEDLINE), Web Science, selecting most referenced related papers last decade. We, therefore, highlight need use expectorated induced specimens routine sample protein diagnose chronic disorders, predict inflammation progression, well monitor effectiveness treatments. Further, we discuss urgent fast reliable methods detect quantify crucial specimens, limitations faced when dealing matrices.

Language: Английский

---