Determination of amino acids and peptides without their pre‐column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(16)

Published: Aug. 1, 2024

This review provides an overview of recent works focusing on the determination amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection ultraviolet (UV) detection, which is most widespread in electromigration techniques, without pre-capillary derivatization. Available options for UV these analytes, such as indirect complexation transition metal ions, in-capillary derivatization are described. Developments field direct UV-absorbing AAs well progress chiral separation A separate section dedicated to on-line sample preconcentration methods combined electrophoresis-UV.

Language: Английский

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Chiral analysis of β‐alanyl‐d,l‐tyrosine and its derivatives and estimation of binding constants of their complexes with 2‐hydroxypropyl‐β‐cyclodextrin by capillary electrophoresis

Journal of Separation Science, Journal Year: 2022, Volume and Issue: 45(17), P. 3328 - 3338

Published: April 24, 2022

Chiral CE methods were developed for the elucidation of l- or d-configuration tyrosine residue in antimicrobial dipeptide β-alanyl-tyrosine (β-Ala-Tyr) isolated from hemolymph larvae fleshfly Neobellieria bullata and evaluation enantiopurity its synthetic isomers (β-Ala-d-Tyr β-Ala-l-Tyr), enantiomers their amidated acetylated derivatives, β-Ala-d,l-Tyr-NH2 N-Ac-β-Ala-d,l-Tyr, respectively. Baseline separations achieved all three pairs enantiomers: (i) β-Ala-d,l-Tyr acidic background electrolyte composed 32/50 mM tris(hydroxymethyl)aminomethane/H3 PO4 , pH 2.5, 20 mg/mL 2-hydroxypropyl-β-cyclodextrin as chiral selector; (ii) consisting 48/50 3.5, 30 2-hydroxypropyl-β-cyclodextrin; (iii) N-Ac-β-Ala-d,l-Tyr alkaline 50/49 Na2 B4 O7 /NaOH, 10.5, 60 2-hydroxypropyl-β-cyclodextrin. From analyses mixed samples β-Ala-Tyr standards β-Ala-l-Tyr β-Ala-d-Tyr, it turned out that was pure l-enantiomer. In addition, average apparent binding constants, Kb actual ionic mobilities complexes above derivatives with determined. These weak, values ranging 11.2 to 79.1 L/mol. Their cationic equal (5.6-9.2) × 10-9 m2 /V/s, anionic (-1.3-1.6) /V/s.

Language: Английский

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Developments in on-line, post separation sample manipulation in the last 22 years: Pharmaceutical and biomedical applications

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2023, Volume and Issue: 235, P. 115654 - 115654

Published: Aug. 16, 2023

Language: Английский

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Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period March 2021 to March 2023

Electrophoresis, Journal Year: 2023, Volume and Issue: 45(1-2), P. 8 - 34

Published: Aug. 21, 2023

This work presents a revision of the main applications capillary electromigration (CE) methods in food analysis and Foodomics. Papers that were published during period March 2021 to 2023 are included. The shows multiple CE have been developed applied analyze different types molecules foods beverages. Namely, amino acids, biogenic amines, heterocyclic peptides, proteins, phenols, polyphenols, pigments, lipids, carbohydrates, vitamins, DNAs, contaminants, toxins, pesticides, additives, residues, small organic inorganic compounds, other minor compounds. In addition, new procedures perform chiral separation for evaluating effects processing as well last developments microchip Foodomics will be also discussed. investigate quality safety, nutritional value, storage, bioactivity included present review work.

Language: Английский

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Portable Analytical Platforms Associated with Chemometrics for Rapid Screening of Whisky Adulteration

Food Analytical Methods, Journal Year: 2022, Volume and Issue: 15(9), P. 2451 - 2461

Published: April 23, 2022

Language: Английский

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