Frontiers in Oncology,
Journal Year:
2022,
Volume and Issue:
12
Published: March 10, 2022
Background
Lymph
node
metastasis
(LNM)
commonly
occurs
in
gastric
cancer
(GC)
and
is
tightly
associated
with
poor
prognosis.
Exosome-mediated
lymphangiogenesis
has
been
considered
an
important
driver
of
LNM.
Whether
exosomes
directly
transmit
the
LNM
phenotype
between
GC
cells
its
mechanisms
remain
elusive.
Methods
A
highly
lymphatic
metastatic
cell
line
(HGC-27-L)
was
established
by
serial
passage
parental
HGC-27
BALB/c
nude
mice.
The
capacities
migration,
invasion
LNM;
fatty
acid
oxidation
(FAO)
levels;
role
exosome-transferred
were
compared
among
HGC-27-L,
primary
AGS.
Exosomes
derived
from
sera
separately
isolated
using
ultracentrifugation
ExoQuick
exosome
precipitation
solution,
characterized
transmission
electron
microscopy,
Nanosight
western
blotting.
Transwell
assay
models
conducted
to
evaluate
vitro
vivo
.
β-oxidation
rate
CPT1
activity
measured
assess
FAO.
CPT1A
inhibitor
etomoxir
used
determine
Label-free
LC-MS/MS
proteome
analysis
screened
differential
protein
profiling
HGC-27-exosomes
AGS-exosomes.
Small
interference
RNAs
YAP
verteporfin
elucidate
mechanism
exosomal
CD44.
TCGA
data
analysis,
immunochemistry
staining
ELISA
performed
analyze
expression
correlation
clinical
significance
CD44/YAP/CPT1A.
Results
FAO
increased
indispensable
for
sustaining
capacity.
Lymphatic
cell-exosomes
conferred
capacity
on
FAO-dependent
way.
Mechanistically,
CD44
identified
be
enriched
a
critical
cargo
regulating
exosome-mediated
transmission,
possibly
modulating
RhoA/YAP/Prox1/CPT1A
signaling
axis.
Abnormal
CD44/YAP/CPT1A
tissues
correlated
each
other
status,
stages,
survival.
Serum
concentration
positively
tumor
burden
lymph
nodes.
Conclusions
We
uncovered
novel
mechanism:
transmits
via
YAP-CPT1A-mediated
reprogramming
perspective
exosomes-transferred
phenotype.
This
provides
potential
therapeutic
targets
non-invasive
biomarker
patients
Journal of Clinical Laboratory Analysis,
Journal Year:
2020,
Volume and Issue:
34(7)
Published: May 17, 2020
Circular
RNAs
(circRNAs)
are
structural
ubiquitous
RNA
molecules.
Accumulating
evidences
have
elucidated
that
circRNAs
play
essential
roles
in
the
pathogenesis
of
diseases
including
cancers.
Exosomal
those
stably
existing
exosomes
and
having
high
clinical
values
as
novel
potential
diagnostic
biomarkers
many
diseases.
Gastrointestinal
(GI)
malignancies,
pancreatic
cancer,
colorectal
hepatocellular
carcinoma
(HCC),
gastric
leading
causes
mortality
worldwide
a
major
global
health
burden.
However,
no
ideal
tumor
screening
early
GI
cancers
currently
available.
Cancers,
Journal Year:
2020,
Volume and Issue:
13(1), P. 84 - 84
Published: Dec. 30, 2020
Exosomes
are
lipid
bilayer
particles
released
from
cells
into
their
surrounding
environment.
These
vesicles
mediators
of
near
and
long-distance
intercellular
communication
affect
various
aspects
cell
biology.
In
addition
to
biological
function,
they
play
an
increasingly
important
role
both
in
diagnosis
as
therapeutic
agents.
this
paper,
we
review
recent
literature
related
the
molecular
composition
exosomes,
paying
special
attention
pathogenesis,
along
with
application
biomarkers
tools.
context,
analyze
potential
use
exosomes
biomedicine,
well
limitations
that
preclude
wider
application.
Cell Communication and Signaling,
Journal Year:
2022,
Volume and Issue:
20(1)
Published: Jan. 3, 2022
Pancreatic
cancer
is
a
highly
malignant
tumor
and,
extremely
difficult
to
diagnose
and
treat.
Metastasis
one
of
the
critical
steps
in
development
uses
cell
communication
mediate
changes
microenvironment.
Small
extracellular
vesicles
(sEVs)-carry
proteins,
nucleic
acids
other
bioactive
substances,
are
important
medium
for
between
cells.
There
two
primary
sVEs-mediated
metastasis:
pancreatic
cells
their
surrounding
microenvironment;
distant
organ
organs
that
promotes
angiogenesis,
reshaping
matrix,
forming
immunosuppressive
environment
ways
form
appropriate
pre-metastasis
niche.
Here,
we
explore
mechanism
localization
metastasis
use
sEVs
as
early
biomarkers
detection
treatment
cancer.
Video
Abstract.
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: Aug. 13, 2024
Glioblastoma-derived
exosomes
(GDEs),
containing
nucleic
acids,
proteins,
fatty
acids
and
other
substances,
perform
multiple
important
functions
in
glioblastoma
microenvironment.
Tumor-derived
serve
as
carriers
of
induce
a
shift
metabolism
towards
oxidative
phosphorylation,
thus
driving
immune
dysfunction
dendritic
cells
(DCs).
Lipid
peroxidation
is
an
characteristic
ferroptosis.
Nevertheless,
it
remains
unclear
whether
GDEs
can
lipid
accumulation
oxidation
to
trigger
ferroptosis
DCs.
In
our
study,
we
investigate
the
impact
on
DCs
by
inhibiting
secretion
through
knocking
down
expression
Rab27a
using
rat
orthotopic
model.
The
results
show
that
reduce
infiltrating
brain
decrease
mature
(mDCs)
levels,
thereby
suppressing
growth.
Mechanistically,
employed
vitro
treatments
bone
marrow-derived
(BMDCs)
with
GDEs.
indicate
viability
mDCs
compared
immature
(imDCs)
via
NRF2/GPX4
pathway.
Overall,
these
findings
provide
new
insights
into
development
immune-suppressive
microenvironment
interaction
Cancers,
Journal Year:
2019,
Volume and Issue:
11(8), P. 1147 - 1147
Published: Aug. 10, 2019
Peritoneal
dissemination
is
a
distinct
form
of
metastasis
in
ovarian
cancer
that
precedes
hematogenic
or
lymphatic
metastasis.
Exosomes
are
extracellular
vesicles
30–150
nm
diameter
secreted
by
different
cell
types
and
internalized
target
cells.
There
emerging
evidence
exosomes
facilitate
the
peritoneal
mediating
intercellular
communication
between
cells
tumor
microenvironment
through
transfer
nucleic
acids,
proteins,
lipids.
Furthermore,
therapeutic
applications
as
drug
cargo
delivery
attracting
research
interest
because
stabilized
circulation.
This
review
highlights
functions
each
process
discusses
their
potential
for
therapeutics.
International Journal of Molecular Sciences,
Journal Year:
2019,
Volume and Issue:
20(12), P. 2970 - 2970
Published: June 18, 2019
The
sharing
of
molecules
function
that
affects
both
tumor
growth
and
neoangiogenesis
with
cells
the
immune
system
creates
a
mutual
interplay
impairs
host's
response
against
progression.
Increasing
evidence
shows
tumors
are
able
to
create
an
immunosuppressive
microenvironment
by
recruiting
specific
cells.
Moreover,
produced
inflammatory
in
milieu
inhibit
development
efficient
cancer
thus
fostering
In
addition,
immunoediting
could
select
less
immunogenic
or
more
resistant
lysis.
this
review,
we
summarize
recent
findings
regarding
immunomodulatory
effects
progression
angiogenic
factor
namely
placental
(PlGF)
address
biological
complex
cytokine.
Different
pathways
innate
adaptive
which,
directly
indirectly,
PlGF
is
involved
promoting
escape
metastasis
will
be
described.
important
for
building
up
vascular
structures
functions.
Although
on
have
been
widely
summarized,
its
functions
modulating
intra-tumoral
highlighted.
agreement
functions,
different
antitumor
strategies
can
envisioned.
