Lipids of Platelet-Rich Fibrin Reduce the Inflammatory Response in Mesenchymal Cells and Macrophages

Cells, Journal Year: 2023, Volume and Issue: 12(4), P. 634 - 634

Published: Feb. 16, 2023

Platelet-rich fibrin (PRF) has a potent anti-inflammatory activity but the components mediating this effect remain unknown. Blood lipids have properties. The question arises whether is also true for lipid fraction of PRF. To answer question, fractions solid and liquid PRF were tested their potential to lower inflammatory response ST2 bone marrow stromal cells primary macrophages exposed IL1β TNFα, LPS, respectively. Cytokine production underlying signalling pathway analysed by RT-PCR, immunoassays, Western blotting. We report here that from substantially lowered cytokine-induced expression IL6, CCL2 CCL5 in cells. Moreover, induced Pam3CSK4, agonist Toll-like receptor (TLR) TLR2, was partially reduced extracts further LPS-induced IL1β, IL6 at transcriptional level. This confirmed showing ability diminish protein level macrophages. Likewise, phosphorylation p38 JNK moderately decreased NFκB-p65 These findings suggest least responsible vitro.

Language: Английский

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Treatment of periodontal intrabony defects using bovine porous bone mineral and guided tissue regeneration with/without platelet‐rich fibrin: A randomized controlled clinical trial

Journal of Periodontology, Journal Year: 2021, Volume and Issue: 92(11), P. 1546 - 1553

Published: Feb. 11, 2021

To investigate the regenerative effect of adjunctive use guided tissue regeneration (GTR), bovine porous bone mineral (BPBM), and platelet-rich fibrin (PRF) in intrabony defects.Fourteen participants were enrolled, for each patient their left right two sides randomized to test group or control group. Only worst defect on side was analyzed. The received GTR, BPBM, PRF, whereas only GTR BPBM. PRF used trial fluid which combined with BPBM form a BPBM-PRF complex. patients followed up by clinical radiographic evaluation 24 months after surgery.Probing depth (PD) significantly less than that at 12 surgery, mean difference ≈ 0.5 0.7 mm. Clinical attachment level (CAL) gain 0.9 mm higher 6 reached 1.0 1.1 surgery. None other parameters differed between groups any time-point surgery.Compared combination complex is more effective clinically, results better outcomes.

Language: Английский

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Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(21), P. 11333 - 11333

Published: Oct. 20, 2021

Chronic inflammation is a pathological process where cells of the mesenchymal lineage become major source inflammatory mediators. Platelet-rich fibrin (PRF) has been shown to possess potent anti-inflammatory activity in macrophages, but its impact on not investigated. The aim this study was, therefore, expose cytokines together with lysates generated from liquid platelet-poor plasma (PPP), cell-rich buffy coat layer (BC; concentrated-PRF or C-PRF), and remaining red clot (RC), following centrifugation blood. Heating PPP generates an albumin gel (Alb-gel) that when mixed back C-PRF produces Alb-PRF. Membranes prepared solid PRF were also subjected lysis. We report here PPP, BC, decreased cytokine-induced expression interleukin 6 (IL6) nitric oxide synthase (iNOS) bone marrow-derived ST2 cells. Consistently, greatly phosphorylation nuclear translocation p65 response caused by Pam3CSK4 was reduced accordingly. Moreover, enhanced mediators IL6 iNOS 3T3-L1 pre-adipocyte cells, CCL5 murine calvarial Surprisingly, effective reducing human gingival fibroblasts HSC2 epithelial data present suggest both exert

Language: Английский

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Hydrogel‐Based Artificial Mucosa Restores Local Immune and Microbial Homeostasis for Treating Ulcerative Colitis

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(22)

Published: March 10, 2023

Abstract Ulcerative colitis (UC) is a common inflammatory bowel disease driven by the dyshomeostasis of intestinal immune system and microbiota, but its treatment options are limited due to impaired epithelial barrier integrity function. Herein, multifunctional biomimetic hydrogel developed as artificial colonic mucosa, which can readily establish bioadhesive at ulcer site restore local microbial homeostasis exert potent anti‐inflammatory effect. The framework generated through thrombin‐mediated activation polymerization fibrinogen that resembles natural blood coagulation mechanism. During this process, antibacterial agent Ag + used for crosslinking between fibrin units dopamine/thiol‐modified hyaluronic acid biopolymers via ‐thiol coordination convey enhanced bioadhesiveness, while interconnected porous network employed encapsulate basic fibroblast growth factor (bFGF) alanyl‐glutamine (ALG) mucosa regeneration. stably adheres protect repair damaged epithelium establishes an microenvironment inducing locoregional M1‐to‐M2 macrophage repolarization eliminating harmful gut flora, act in cooperative manner substantially alleviate UC symptoms. here presents promising approach effective therapy clinics.

Language: Английский

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Periodontal regeneration using platelet‐rich fibrin. Furcation defects: A systematic review with meta‐analysis

Periodontology 2000, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Abstract The objective of the study was to compare treatment outcomes periodontal furcation defects by using platelet‐rich fibrin (PRF) with other commonly utilized modalities. eligibility criteria comprised randomized controlled trials (RCTs) comparing clinical PRF those modalities for defects. Studies were classified into 11 categories in 3 different groups as follows: Group I (addition PRF): (1) open flap debridement (OFD) alone versus OFD/PRF, (2) OFD/bone graft (OFD/BG) OFD/BG/PRF; II (comparative studies (3) OFD/BG (4) OFD/collagen membrane (5) OFD/PRP (6) OFD/rhBMP2 OFD/PRF; and III biomaterial/biomolecule OFD/PRF … (7) OFD/PRF/BG, (8) OFD/PRF/amniotic (AM), (9) OFD/PRF/metformin, (10) OFD/PRF/bisphosphonates, (11) OFD/PRF/statins. Weighted means forest plots calculated reduction probing pocket depth (PPD), gain vertical horizontal attachment levels (VCAL HCAL), bone (VBL, HBL), radiographic fill (RBF). From 45 articles identified, 21 RCTs reporting on class furcations included. use OFD/BG/PRF statistically significantly reduced PPD improved VCAL HCAL when compared OFD or OFD/BG, respectively. comparison between OFD/CM, OFD/PRP, led similar all investigated parameters, including a PPD, VCAL/HCAL gain, RBF. additional incorporation BG only mildly outcomes, whereas addition AM outcomes. small biomolecules such metformin, bisphosphonates, statins significant improvements VCAL, alone. Noteworthy, very high heterogeneity found studies. alone, being observed and/or OFD/rhBMP2. Future research geared toward better understanding potential ways enhance regenerative properties various may prove valuable future applications. histological investigating human is largely needed. conjunction values, yielding comparable used biomaterials. combination grafts offer benefits, thus warranting investigation.

Language: Английский

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Immunoregulatory role of platelet derivatives in the macrophage-mediated immune response

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 25, 2024

Background Macrophages are innate immune cells that display remarkable phenotypic heterogeneity and functional plasticity. Due to their involvement in the pathogenesis of several human conditions, macrophages considered be an attractive therapeutic target. In line with this, platelet derivatives have been successfully applied many medical fields as active participants immunity, cooperation between platelets is essential. this context, aim review compile current evidence regarding effects on phenotype functions identify advantages shortcomings for feasible future clinical applications. Methods A total 669 articles were identified during systematic literature search performed PubMed Web Science databases. Results 27 met inclusion criteria. Based published findings, may play important role inducing a dynamic M1/M2 balance promoting timely M1-M2 shift. However, differences procedures polarization occasional lack information, makes reproducibility comparison results extremely challenging. Furthermore, understanding those derived from animal models, taking into account peculiarities tissue resident ontogeny seem essential design new strategies. Conclusion Research combination provides relevant information function mechanisms response.

Language: Английский

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Autologous platelet concentrates after third molar extraction: A systematic review

Periodontology 2000, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Surgical removal of impacted mandibular third molars is often followed by postoperative sequelae like pain, swelling, trismus, etc. This systematic review explored the benefits applying different autologous platelet concentrates (APCs) in extraction socket molars. For this review, PubMed, EMBASE, Web Science, and Scopus have been utilized, initially yielding 544 papers. The search was narrowed to randomized controlled trials (RCTs, n = 59) published before 2024, all comparing outcome APCs surgically removed with unassisted healing (blood clot). Most RCTs primarily assessed impact on sequelae. Some looked at soft‐ hard‐tissue healing. Eleven studies used PRP, three PRGF, 45 L‐PRF. A detailed analysis revealed a large heterogeneity between rendering meta‐analysis impossible. Moreover, risk bias considered high. In majority RCTs, application an APC resulted statistically significant reductions (lower pain intensity, lower consumption analgesics, less edema, incidence trismus alveolar osteitis), as well faster soft tissue healing, qualitatively quantitatively better bone minority reported differences periodontal parameters distally from second molar.

Language: Английский

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Liquid PRF Reduces the Inflammatory Response and Osteoclastogenesis in Murine Macrophages

Frontiers in Immunology, Journal Year: 2021, Volume and Issue: 12

Published: April 9, 2021

Macrophage activation and osteoclastogenesis are hallmarks of inflammatory osteolysis may be targeted by the local application liquid platelet-rich fibrin (PRF). Liquid PRF is produced a hard spin blood in absence clot activators anticoagulants, thereby generating an upper platelet-poor plasma (PPP) layer, cell-rich buffy coat layer (BC; termed concentrated-PRF or C-PRF), remaining red (RC) layer. Heating PPP has been shown to generate albumin gel (Alb-gel) that when mixed back with C-PRF generates Alb-PRF having extended working properties implanted vivo . Evidence demonstrated traditional solid holds potent anti-inflammatory capacity reduces osteoclastogenesis. Whether capable also suppressing response formation osteoclasts remains open. In present study, RAW 264.7 primary macrophages were exposed lipopolysaccharides (LPS), lactoferrin, agonists Toll-like receptors (TLR3 TLR7) presence lysates prepared freeze-thawing PPP, BC, Alb-gel, RC. For osteoclastogenesis, receptor activator nuclear factor kappa B ligand (RANKL), macrophage colony-stimulating (M-CSF), human transforming growth factor-β1 (TGF-β1) RC hemoglobin. We show here it mainly from BC consistently reduced agonist-induced expression interleukin 6 (IL6) cyclooxygenase-2 (COX2) macrophages, as determined RT-PCR immunoassay. With respect but RC, similar hemoglobin, osteoclast marker genes tartrate-resistant acid phosphatase (TRAP) cathepsin K, well TRAP histochemical staining. These findings suggest vitro heat-sensitive activity goes along inhibition

Language: Английский

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Does the Use of Injectable Platelet-Rich Fibrin After Arthrocentesis for Disc Displacement Without Reduction Improve Clinical Outcomes?

Journal of Oral and Maxillofacial Surgery, Journal Year: 2023, Volume and Issue: 81(6), P. 689 - 697

Published: March 13, 2023

Language: Английский

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Do blood concentrates influence inflammatory signs and symptoms after mandibular third molar surgery? A systematic review and network meta-analysis of randomized clinical trials

Clinical Oral Investigations, Journal Year: 2023, Volume and Issue: 27(12), P. 7045 - 7078

Published: Oct. 26, 2023

Language: Английский

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