Liver Tissue Engineering: Challenges and Opportunities DOI
Tarun Agarwal, Bhuvaneshwaran Subramanian, Tapas K. Maiti

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2019, Volume and Issue: 5(9), P. 4167 - 4182

Published: July 29, 2019

Liver tissue engineering aims at the possibility of reproducing a fully functional organ for treatment acute and chronic liver disorders. Approaches in this field endeavor to replace transplantation (gold standard diseases clinical setting) with vitro developed constructs. However, complexity microarchitecture functionality along limited supply cellular components pose numerous challenges. This review provides comprehensive outlook onto how physicochemical, mechanobiological, spatiotemporal aspects substrates could be tuned address current challenges field. We also highlight strategic advancements made so far development artificial tissue. further showcase currently available prototypes research trials, which shows hope future engineering.

Language: Английский

Induced Osteogenesis in Plants Decellularized Scaffolds DOI Creative Commons
Jennifer Lee, Hyerin Jung, Narae Park

et al.

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: Dec. 27, 2019

Abstract A three-dimensional (3D) culture system that closely replicates the in vivo microenvironment of calcifying osteoid is essential for vitro cultivation bone-like material. In this regard, 3D cellulose constructs plants may well serve as scaffolds to promote growth and differentiation osteoblasts culture. Our aim study was generate tissue by seeding pluripotent stem cells (hiPSCs), stimulated differentiate culture, onto decellularised various plants. We then assessed expression levels pertinent cellular markers degrees calcium-specific staining gauge technical success. Apple scaffolding bearing regular pores 300 μm seemed provide best construct. The thus generated implantable a rat calvarial defect model where if helped form calcified tissue. Depending on regularity sizing scaffold pores, approach readily facilitates production mineralized bone.

Language: Английский

Citations

107
Tissue Engineering in Liver Regenerative Medicine: Insights into Novel Translational Technologies DOI Creative Commons
Zahra Heydari, Mustapha Najimi, Hamed Mirzaei

et al.

Cells, Journal Year: 2020, Volume and Issue: 9(2), P. 304 - 304

Published: Jan. 27, 2020

Organ and tissue shortage are known as a crucially important public health problem unfortunately small percentage of patients receive transplants. In the context emerging regenerative medicine, researchers trying to regenerate replace different organs tissues such liver, heart, skin, kidney. Liver engineering (TE) enables us reproduce restore liver functions, fully or partially, which could be used in treatment acute chronic disorders and/or generate an appropriate functional organ can transplanted employed extracorporeal device. this regard, variety techniques (e.g., fabrication technologies, cell-based microfluidic systems and, devices) applied medicine. Common TE based on allocating stem cell-derived hepatocyte-like cells primary hepatocytes within three-dimensional structure leads improvement their survival rate phenotype. Taken together, new findings indicated that developing engineering-based pave way for better liver-related disorders. Herein, we summarized novel technologies medicine future applications clinical settings.

Language: Английский

Citations

93
Liver organoid as a 3D in vitro model for drug validation and toxicity assessment DOI
Anastasia Brooks, Xiaowen Liang, Yonglong Zhang

et al.

Pharmacological Research, Journal Year: 2021, Volume and Issue: 169, P. 105608 - 105608

Published: April 20, 2021

Language: Английский

Citations

72
Hydrogels derived from decellularized liver tissue support the growth and differentiation of cholangiocyte organoids DOI Creative Commons
Jorke Willemse, Gilles van Tienderen, Eline V. A. van Hengel

et al.

Biomaterials, Journal Year: 2022, Volume and Issue: 284, P. 121473 - 121473

Published: March 24, 2022

Human cholangiocyte organoids are promising for regenerative medicine applications, such as repair of damaged bile ducts. However, typically cultured in mouse tumor-derived basement membrane extracts (BME), which is poorly defined, highly variable and limits the direct clinical applications patients. Extracellular matrix (ECM)-derived hydrogels prepared from decellularized human or porcine livers attractive alternative culture substrates. Here, expansion liver ECM(LECM)-derived described. These support proliferation maintain cholangiocyte-like phenotype. The use LECM does not significantly alter expression selected genes proteins, marker cytokeratin-7, no species-specific effect found between hydrogels. Proliferation rates lower, but differentiation capacity towards hepatocyte-like cells altered by presence tissue-specific ECM components. Moreover, ICO a dynamic set up without need laborious static hydrogel domes. Liver can successfully replace BME potentially unlock full potential organoids.

Language: Английский

Citations

69
Decellularized ECM hydrogels: prior use considerations, applications, and opportunities in tissue engineering and biofabrication DOI
Jacqueline Kort-Mascort, Salvador Flores-Torres, Omar Peza-Chavez

et al.

Biomaterials Science, Journal Year: 2022, Volume and Issue: 11(2), P. 400 - 431

Published: Dec. 2, 2022

Decellularized extracellular matrix hydrogels are tissue-derived materials that with proper processing can be used for tissue engineering applications and to build microenvironments in vitro . Figure was partly created Servier Medical Art.

Language: Английский

Citations

45
Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids DOI
Beatriz S. Moura, Maria V. Monteiro, Luís P. Ferreira

et al.

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(29)

Published: May 22, 2022

Abstract The extracellular matrix plays a critical role in bioinstructing cellular self‐assembly and spatial (re)configuration processes that culminate human organoids vitro generation maturation. Considering the importance of supporting matrix, herein is showcased most recent advances bioengineering decellularized tissue hydrogels for generating assembloids. Key design blueprints, characterization methodologies, processing toolboxes are comprehensively discussed light current advances. Such enabling approaches provide grounds engineering next‐generation tissue‐specific with close‐to‐native biomolecular signatures user‐tailored biophysical properties may potentiate physiomimetic potential. In forward looking perspective, combination increasingly complex multicellular assemblies bottom‐up cell technologies unravel unprecedented tissue‐like physiological responses further advance exploitation assembloids as disease surrogates or patient‐tailored living therapeutics.

Language: Английский

Citations

43
Revolutionizing Disease Modeling: The Emergence of Organoids in Cellular Systems DOI Creative Commons

Rita Silva-Pedrosa,

António J. Salgado, Pedro Eduardo Ferreira

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(6), P. 930 - 930

Published: March 18, 2023

Cellular models have created opportunities to explore the characteristics of human diseases through well-established protocols, while avoiding ethical restrictions associated with post-mortem studies and costs researching animal models. The capability cell reprogramming, such as induced pluripotent stem cells (iPSCs) technology, solved complications embryonic (hESC) usage. Moreover, iPSCs made significant contributions for medicine, in diagnosis, therapeutic regenerative medicine. two-dimensional (2D) allowed monolayer cellular culture vitro; however, they were surpassed by three-dimensional (3D) system. 3D provides higher cell–cell contact a multi-layered culture, which more closely respects morphology polarity. It is tightly able resemble conditions vivo closer approach architecture tissues, organoids. Organoids are structures that mimic function native tissues. They generated vitro from or differentiated cells, epithelial neural used study organ development, disease modeling, drug discovery. become powerful tool understanding molecular mechanisms underlying physiology, providing new insights into pathogenesis cancer, metabolic diseases, brain disorders. Although organoid technology up-and-coming, it also has some limitations require improvements.

Language: Английский

Citations

37
Exploring the evolution of tissue engineering strategies over the past decade: From cell-based strategies to gene-activated matrix DOI Creative Commons
Yasaman Esmaeili, Elham Bidram, Ashkan Bigham

et al.

Alexandria Engineering Journal, Journal Year: 2023, Volume and Issue: 81, P. 137 - 169

Published: Sept. 14, 2023

The advancement of tissue engineering for regenerating injured tissues and organs has progressed significantly in recent years. Various techniques have been used to modify the cells' microenvironments targeted via their extracellular environment achieving these aims. 3D structured scaffolds alone or combined with bioactive molecules genes cells hold great promise development functional engineered tissues. As an emerging state-of-the-art technology this field, integrating gene therapy, known as gene-activated matrix (GAM), gained immense attention a promising approach restoring damaged dysfunctional tissues' function structure. Nonetheless, fabricating GAMs low cytotoxicity, high transfection efficiency, long-term delivery efficiency is still challenging. Here we provide complete overview different approaches ongoing preclinical research trials. Moreover, GAM strategy focus on development, faithful application, future prospects repair regeneration replacement assayed. challenges regenerative medicine are also presented. Eventually, propose that offer basic mechanistic infrastructure "tissue engineering" pave way clinical translation achieve personalized medicine.

Language: Английский

Citations

34
Progress in biomaterials inspired by the extracellular matrix DOI Creative Commons

Zhuolin Chen,

Chengcheng Du, Senrui Liu

et al.

Giant, Journal Year: 2024, Volume and Issue: 19, P. 100323 - 100323

Published: July 10, 2024

Inspired by the extracellular matrix (ECM), biomaterials have emerged as promising strategies in biomedical research and engineering domain, offering unique characteristics for tissue regeneration, drug delivery, therapeutic interventions, cellular investigations. The ECM, a dynamic network structure secreted various cells, primarily comprises diverse proteins capable of facilitating tissue-ECM signaling regulatory functions through its rich array bioactive substances multi-level structural properties. Drawing inspiration from intricate biochemical composition natural researchers developed to encapsulate these features create biomimetic microenvironments, such electrospinning, hydrogels/hydrogel microspheres, decellularized ECM(dECM), ECM-mimicking peptides. Furthermore, mimicking ECM components, ECM-inspired exhibit varying degrees functionalization, including providing support, cell adhesion, signal transduction, mitigating immune responses, remodeling. In summary, advancements offer significant promise addressing key challenges fields engineering, regenerative medicine, delivery.

Language: Английский

Citations

15
Cervical extracellular matrix hydrogel optimizes tumor heterogeneity of cervical squamous cell carcinoma organoids DOI Creative Commons
Haonan Song, Hai-Tang Jiang, Weichu Hu

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(20)

Published: May 15, 2024

Cervical cancer, primarily squamous cell carcinoma, is the most prevalent gynecologic malignancy. Organoids can mimic tumor development in vitro, but current Matrigel inaccurately replicates tissue-specific microenvironment. This limitation compromises accurate representation of heterogeneity. We collected para-cancerous cervical tissues from patients diagnosed with carcinoma (CSCC) and prepared uterine cervix extracellular matrix (UCEM) hydrogels. Proteomic analysis UCEM identified several signaling pathways including human papillomavirus, phosphatidylinositol 3-kinase-AKT, receptor. Secreted proteins like FLNA, MYH9, HSPA8, EEF1A1 were present, indicating successfully maintained proteins. provided a tailored microenvironment for CSCC organoids, enabling formation growth while preserving tumorigenic potential. RNA sequencing showed UCEM-organoids exhibited greater similarity to native reflected heterogeneity by exhibiting CSCC-associated virus protein-cytokine, nuclear factor κB, necrosis factor, oncogenes EGR1, FPR1, IFI6. Moreover, developed chemotherapy resistance. Our research provides insights into advanced organoid technology through

Language: Английский

Citations

12