Journal of Extracellular Vesicles,
Journal Year:
2023,
Volume and Issue:
12(2)
Published: Feb. 1, 2023
Extracellular
vesicles
(EVs)
are
cell-released,
heterogenous
nanoparticles
that
play
important
roles
in
(patho)physiological
processes
through
intercellular
communication.
EVs
often
depicted
as
having
a
single
lipid
bilayer,
but
many
studies
have
demonstrated
the
existence
of
multilayered
EVs.
There
has
been
minimal
inquiry
into
differences
between
unilamellar
and
multilamellar
terms
biogenesis
mechanisms
functional
effects.
This
commentary
speculates
on
potential
causes
serves
call
to
action
for
research
community
unravel
complex
layers
European Journal of Cell Biology,
Journal Year:
2022,
Volume and Issue:
101(3), P. 151226 - 151226
Published: April 15, 2022
Cells
release
membrane-delimited
particles
into
the
environment.
These
are
called
"extracellular
vesicles"
(EVs),
and
EVs
present
in
fluids
contacting
cells,
including
body
conditioned
culture
media.
Because
change
contribute
to
health
disease,
have
become
a
hot
topic.
From
thousands
of
papers
now
published
on
annually,
one
easily
gets
impression
that
provide
biomarkers
for
all
diseases,
carriers
relevant
biomolecules
omnipotent
therapeutics.
At
same
time,
heterogeneous,
elusive
difficult
study
due
their
physical
properties
complex
composition
This
overview
addresses
current
challenges
encountered
when
working
with
EVs,
how
we
envision
most
these
will
be
overcome
near
future.
Right
now,
an
infrastructure
is
being
developed
improve
reproducibility
EV
measurement
results.
comprises
expert
task
forces
International
Society
Extracellular
Vesicles
(ISEV)
developing
guidelines
recommendations,
instrument
calibration,
standardized
transparent
reporting,
education.
Altogether,
developments
support
credibility
research
by
introducing
robust
reproducibility,
which
prerequisite
understanding
biological
significance
biomarker
potential.
Acta Pharmaceutica Sinica B,
Journal Year:
2023,
Volume and Issue:
13(8), P. 3300 - 3320
Published: March 7, 2023
Extracellular
vesicles
(EVs)
are
phospholipid
bilayer
actively
secreted
by
cells,
that
contain
a
variety
of
functional
nucleic
acids,
proteins,
and
lipids,
important
mediums
intercellular
communication.
Based
on
their
natural
properties,
EVs
can
not
only
retain
the
pharmacological
effects
source
cells
but
also
serve
as
delivery
carriers.
Among
them,
plant-derived
nanovesicles
(PNVs)
characterized
disease
therapeutics
with
many
advantages
such
simplicity,
safety,
eco-friendliness,
low
cost,
toxicity
due
to
abundant
resources,
large
yield,
risk
immunogenicity
in
vivo.
This
review
systematically
introduces
biogenesis,
isolation
methods,
physical
characterization,
components
PNVs,
describes
administration
cellular
uptake
therapeutic
agents.
We
highlight
potential
PNVs
agents
drug
carriers,
including
anti-inflammatory,
anticancer,
wound
healing,
regeneration,
antiaging
properties
well
use
treatment
liver
COVID-19.
Finally,
immunogenicity,
current
clinical
application,
possible
challenges
future
development
were
analyzed.
expect
functions
be
further
explored
promote
translation,
thereby
facilitating
new
framework
for
human
diseases.
Frontiers in Immunology,
Journal Year:
2023,
Volume and Issue:
14
Published: Jan. 24, 2023
In
the
last
few
decades,
practical
use
of
stem
cells
(SCs)
in
clinic
has
attracted
significant
attention
regenerative
medicine
due
to
ability
these
proliferate
and
differentiate
into
other
cell
types.
However,
recent
findings
have
demonstrated
that
therapeutic
capacity
SCs
may
also
be
mediated
by
their
secrete
biologically
active
factors,
including
extracellular
vesicles
(EVs).
Such
submicron
circular
membrane-enveloped
released
from
surface
harbour
bioactive
cargo
form
proteins,
lipids,
mRNA,
miRNA,
regulatory
factors.
Notably,
growing
evidence
indicated
EVs
transfer
content
recipient
greatly
modulate
functional
fate.
Thus,
they
been
recently
envisioned
as
a
new
class
paracrine
factors
cell-to-cell
communication.
Importantly,
activity
immune
system,
playing
an
important
role
regulation
inflammation,
exhibiting
broad
spectrum
immunomodulatory
promotes
transition
pro-inflammatory
pro-regenerative
environment
site
tissue
injury.
Consequently,
interest
is
placed
on
attempts
utilize
clinical
applications
inflammatory-related
dysfunctions
potential
next-generation
alternative
cell-based
approaches.
this
review
we
will
discuss
current
knowledge
biological
properties
SC-derived
EVs,
with
special
focus
inflammatory
response.
We
address
several
disease
models,
vitro
vivo
preclinical,
well
studies.
Finally,
highlight
perspectives
future
challenges
emerging
EV-based
strategies
inflammation-related
diseases
treatment.
Journal of Extracellular Vesicles,
Journal Year:
2023,
Volume and Issue:
12(2)
Published: Feb. 1, 2023
Extracellular
vesicles
(EVs)
are
cell-released,
heterogenous
nanoparticles
that
play
important
roles
in
(patho)physiological
processes
through
intercellular
communication.
EVs
often
depicted
as
having
a
single
lipid
bilayer,
but
many
studies
have
demonstrated
the
existence
of
multilayered
EVs.
There
has
been
minimal
inquiry
into
differences
between
unilamellar
and
multilamellar
terms
biogenesis
mechanisms
functional
effects.
This
commentary
speculates
on
potential
causes
serves
call
to
action
for
research
community
unravel
complex
layers
