Applications of plant‐derived extracellular vesicles in medicine DOI Creative Commons

Yawen Zhu,

Junqi Zhao, Haoran Ding

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 20, 2024

Plant-derived extracellular vesicles (EVs) are promising therapeutic agents owing to their natural abundance, accessibility, and unique biological properties. This review provides a comprehensive exploration of the potential plant-derived EVs emphasizes anti-inflammatory, antimicrobial, tumor-inhibitory effects. Here, we discussed advancements in isolation purification techniques, such as ultracentrifugation size-exclusion chromatography, which critical for maintaining functional integrity these nanovesicles. Next, investigated diverse administration routes carefully weighed respective advantages challenges related bioavailability patient compliance. Moreover, elucidated multifaceted mechanisms action EVs, including roles anti-inflammation, antioxidation, antitumor activity, modulation gut microbiota. We also impact on specific diseases cancer inflammatory bowel disease, highlighting importance addressing current production scalability, regulatory compliance, immunogenicity. Finally, proposed future research directions optimizing EV extraction developing targeted delivery systems. Through efforts, envision seamless integration into mainstream medicine, offering safe potent alternatives across various medical disciplines.

Language: Английский

Research status and challenges of plant-derived exosome-like nanoparticles DOI Open Access
Chunmei Bai,

J Ping Liu,

Xumin Zhang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 174, P. 116543 - 116543

Published: April 11, 2024

In recent years, there has been an increasing number of related studies on exosomes. Most have focused exosomes derived from mammals, confirming the important role that play in cell communication. Plants, as a natural ingredient, plant-derived confirmed to similar structures and functions mammalian-derived Plant-derived exosome-like nanoparticles (PELNs) are lipid bilayer membrane nanovesicles containing bioactive constituents such miRNA, mRNA, protein, lipids obtained plant cells, can participate intercellular communication mediate transboundary communication, high bioavailability low immunogenicity, relatively safe, shown maintaining homeostasis preventing, treating variety diseases. this review, we describe biogenesis, isolation purification methods, structural composition, stability, safety, function PELNs challenges. The anti-inflammatory, antioxidant, antitumor drug delivery mainly described, status research exosome Chinese herbal medicines is outlined. Overall, summarized importance latest results field provided theoretical basis for future clinical application PELNs.

Language: Английский

Citations

25
Isolation of plant-derived exosome-like nanoparticles (PDENs) from Solanum nigrum L. berries and Their Effect on interleukin-6 expression as a potential anti-inflammatory agent DOI Creative Commons

Natasya Emmanuela,

Daisy Ramadhani Muhammad, Iriawati Iriawati

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(1), P. e0296259 - e0296259

Published: Jan. 4, 2024

Inflammation is a temporary response of the immune system that can be treated using common anti-inflammatory drugs. However, prolonged use these drugs increases risk adverse side effects. Accordingly, there an increasing need for alternative treatments inflammation with fewer Exosomes are extracellular vesicles secreted by most eukaryotic cells and have been studied as candidate cell-free therapy inflammatory diseases due to their immunomodulatory properties. In recent years, focus exosome research has shifted from animal cell-derived exosomes plant-derived exosome-like nanoparticles (PDENs). Plant-derived (PDENs) easier obtain, minimal safety concerns, produced in higher quantities lower cost than derived cells. this study, isolation analysis potential PDENs black nightshade berries ( Solanum nigrum L.) were carried out. The results characterization showed had spherical morphology, measuring around 107 nm zeta -0.6 mV, protein concentration 275.38 μg/mL. also shown internalized RAW264.7 macrophage cell line after 2 hours incubation no cytotoxicity effect up 2.5 Furthermore, exposure several doses LPS-stimulated significantly decreased expression pro-inflammatory cytokine gene IL-6, well IL-6 97,28%. GC-MS presence neral, monoterpene compound known properties, which may contribute activity isolated L. berries. Taken together, present study was first isolate characterize demonstrated PDEN suppressing production

Language: Английский

Citations

23
Bioactive compounds and biological functions of medicinal plant-derived extracellular vesicles DOI Creative Commons
Siyu Hao,

Hongyu Yang,

Jiaojiao Hu

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 200, P. 107062 - 107062

Published: Jan. 9, 2024

Extracellular vesicles (EVs) are tiny lipid bilayer-enclosed membrane particles released from a variety of cell types into the surrounding environment. These EVs have massive participated in cell-to-cell communication and interspecies communication. In recent years, plant-derived extracellular (PDEVs) "exosome-like" populations found distinct plants attracted widespread attention. Especially, research on medicinal (MPDEVs) increasing, which considered kind promising natural compound. This review summarizes current knowledge MPDEVs terms bioactive compounds, including small RNA, protein, lipid, metabolite, been surface and/or lumen MPDEVs. Moreover, both vitro vivo experiments shown that exert broad biomedical functions, such as anti-inflammatory, anticancer, antioxidant, modulate microbiota, etc. may be better substitute than animal-derived (ADEVs) because safety biocompatibility future.

Language: Английский

Citations

18
Plant and mammalian-derived extracellular vesicles: a new therapeutic approach for the future DOI Creative Commons

Ibrahima Mamadou Sall,

Tabaran Alexandru Flaviu

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Sept. 13, 2023

Background: In recent years, extracellular vesicles have been recognized as important mediators of intercellular communication through the transfer active biomolecules (proteins, lipids, and nucleic acids) across plant animal kingdoms considerable roles in several physiological pathological mechanisms, showing great promise new therapeutic strategies for a variety pathologies. Methods: this study, we carefully reviewed numerous articles published over last few decades on general knowledge vesicles, their application therapy various pathologies, prospects an approach future. Results: The discovery characterization (EVs) diverse origins biogenesis altered current paradigm communication, opening up diagnostic perspectives. Research into these EVs released by mammalian cells has revealed involvement number such embryonic development, immune response, tissue regeneration, cancer. They are also being studied potential biomarkers disease diagnosis vectors drug delivery. Conclusion: Nanovesicles represent powerful tools bioactive molecules. Their molecular composition functions can vary according to origin (plant mammalian), so formation, composition, biological open way applications which is arousing growing interest scientific community. Clinical Trial Registration: ClinicalTrials.gov identifier: NCT03608631.

Language: Английский

Citations

25
Isolation and characterization of plant-derived exosome-like nanoparticles from Carica papaya L. fruit and their potential as anti-inflammatory agent DOI Creative Commons
Iriawati Iriawati,

Safira Vitasasti,

Fatimah Nur Azmi Rahmadian

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0304335 - e0304335

Published: July 3, 2024

Inflammation is an immune system response that identifies and eliminates foreign material. However, excessive persistent inflammation could disrupt the healing process. Plant-derived exosome-like nanoparticles (PDENs) are a promising candidate for therapeutic application because they safe, biodegradable biocompatible. In this study, papaya PDENs were isolated by PEG6000-based method characterized dynamic light scattering (DLS), transmission Electron Microscopy (TEM), bicinchoninic acid (BCA) assay method, GC-MS analysis, total phenolic content (TPC) 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. For in vitro test, we conducted internalization toxicity assessment, determination of nitrite concentration, assessed expression inflammatory cytokine genes using qRT-PCR RAW 264.7 cells. vivo was induced caudal fin amputation followed analysis macrophage neutrophil migration zebrafish (Danio rerio) larvae. The result showed can be well optimized differential centrifugation with addition 30 ppm pectolyase, 15% PEG, 0.2 M NaCl, which exhibited cup-shaped spherical morphological structure average diameter 168.8±9.62 nm. storage stable aquabidest 25 mM trehalose solution at -20˚C until fourth week. TPC estimation all ages did not show significant change, while DPPH test change second major compounds contained Papaya 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP). internalized non-cytotoxic to Moreover, LPS-induced cells treated decrease NO production downregulation mRNA pro-inflammatory (IL-1B IL-6) upregulation anti-inflammatory gene (IL-10). addition, tests on inhibition cell migration. These findings suggest possesses properties.

Language: Английский

Citations

11
Modulation of alveolar macrophage and mitochondrial fitness by medicinal plant-derived nanovesicles to mitigate acute lung injury and viral pneumonia DOI Creative Commons

Lusha Ye,

Yanan Gao, Simon Wing Fai Mok

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 18, 2024

Acute lung injury (ALI) is generally caused by severe respiratory infection and characterized overexuberant inflammatory responses inefficient pathogens-containing, the two major processes wherein alveolar macrophages (AMs) play a central role. Dysfunctional mitochondria have been linked with distorted hence disorders, but few treatments are currently available to correct these defects. Plant-derive nanovesicles gained significant attention because of their therapeutic potential, targeting cells underlying mechanism remain elusive. We herein prepared from Artemisia annua, well-known medicinal plant multiple attributes involving anti-inflammatory, anti-infection, metabolism-regulating properties. By applying three mice models acute bacterial endotoxin, influenza A virus (IAV) SARS-CoV-2 pseudovirus respectively, we showed that Artemisia-derived (ADNVs) substantially alleviated immunopathology raised survival rate challenged mice. Macrophage depletion adoptive transfer studies confirmed requirement AMs for ADNVs effects. identified gamma-aminobutyric acid (GABA) enclosed in vesicles molecular effector mediating regulatory roles ADNVs. Specifically, GABA acts on through receptors, promoting mitochondrial gene programming bioenergy generation, reducing oxidative stress signals, thereby enhancing adaptability inflammation resolution. Collectively, this study identifies promising nanotherapeutics alleviating pathology, elucidates whereby canonical neurotransmitter modifies resume tissue homeostasis, which may broader implications treating critical pulmonary diseases such as COVID-19.

Language: Английский

Citations

8
Advances in the study of plant-derived extracellular vesicles in the skeletal muscle system DOI Creative Commons

Xinning Mao,

Tenghui Li, Weihui Qi

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 204, P. 107202 - 107202

Published: May 3, 2024

Plant-derived extracellular vesicles (PDEV) constitute nanoscale entities comprising lipids, proteins, nucleic acids and various components enveloped by the lipid bilayers of plant cells. These play a crucial role in facilitating substance information transfer not only between cells but also across different species. Owing to its safety, stability, abundance raw materials, this has found extensive utilization recent years within research endeavors aimed at treating diseases. This article provides an overview pathways biological characteristics PDEV, along with prevalent methods employed for isolation, purification, storage. Furthermore, we comprehensively outline therapeutic implications diverse sources PDEV musculoskeletal system disorders. Additionally, explore as platforms engineering drug carriers, aiming delve deeper into significance potential contributions realm system.

Language: Английский

Citations

6
Liver cirrhosis: molecular mechanisms and therapeutic interventions DOI Creative Commons
Zihe Dong, Yeying Wang, Weilin Jin

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 17, 2024

Liver cirrhosis is the end-stage of chronic liver disease, characterized by inflammation, necrosis, advanced fibrosis, and regenerative nodule formation. Long-term inflammation can cause continuous damage to tissues hepatocytes, along with increased vascular tone portal hypertension. Among them, fibrosis necessary stage essential feature cirrhosis, effective antifibrosis strategies are commonly considered key treating cirrhosis. Although different therapeutic aimed at reversing or preventing have been developed, effects not be more satisfactory. In this review, we discussed abnormal changes in microenvironment that contribute progression highlighted importance recent strategies, including lifestyle improvement, small molecular agents, traditional Chinese medicine, stem cells, extracellular vesicles, gut remediation, regulate Meanwhile, for nanoparticles discussed, as their possible underlying broad application prospects ameliorating Finally, also reviewed major challenges opportunities nanomedicine‒biological environment interactions. We hope review will provide insights into pathogenesis mechanisms thus facilitating new methods, drug discovery, better treatment

Language: Английский

Citations

6
Plant-Derived Extracellular Vesicles as a Promising Anti-Tumor Approach: A Comprehensive Assessment of Effectiveness, Safety, and Mechanisms DOI
Qiang Wei, Jing Li,

Rui Ruan

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 130, P. 155750 - 155750

Published: May 19, 2024

Language: Английский

Citations

5
Solanum lycopersicum derived exosome-like nanovesicles alleviate restenosis after vascular injury through the Keap1/Nrf2 pathway DOI Creative Commons
Hechen Shen, Meng Zhang, Dachang Liu

et al.

Food & Function, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Exosome-like nanovesicles derived from Solanum lycopersicum show potential to inhibit vascular restenosis. The underlying mechanism involves activation of the Keap1/Nrf2 signaling pathway by miRNA164a/b-5p.

Language: Английский

Citations

4