Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown
Published: Jan. 1, 2023
Language: Английский
Toxins, Journal Year: 2022, Volume and Issue: 14(11), P. 742 - 742
Published: Oct. 29, 2022
Mycotoxins can occur naturally in a variety of agriculture products, including cereals, feeds, and Chinese herbal medicines (TCMs), via pre- post-harvest contamination are regulated worldwide. However, risk mitigation by monitoring for multiple mycotoxins remains challenge using existing methods due to their complex matrices. A multi-toxin method 22 (aflatoxin B1, B2, G1, G2, M1, M2; ochratoxin A, B, C; Fumonisin B3; 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, diace-toxyscirpenol, HT-2, T-2, deepoxy-deoxynivalenol, deoxynivalenol, neosolaniol, zearalenone, sterigmatocystin) centrifugation-assisted solid-phase extraction (SPE) clean-up prior ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis Arecae Semen its processed products was developed validated. Several experimental parameters affecting the efficiency were systematically optimized. The results indicated good linearity range 0.1–1000 μg/kg (r2 > 0.99), low limits detection (ranging from 0.04 1.5 μg/kg), acceptable precisions, satisfactory recoveries selected mycotoxins. validated then applied investigate mycotoxin levels Areca catechu products. frequently contaminating aflatoxins (AFs), average level number co-occurring slices (Binlangpian) higher than those commercially whole Tostum (Jiaobinlang). Sterigmatocystin detected 5 out 30 slices. None investigated pericarpium (Dafupi). demonstrated that SPE coupled with UHPLC-MS/MS be useful tool
Language: Английский
Citations
6
Heliyon, Journal Year: 2023, Volume and Issue: 9(6), P. e17027 - e17027
Published: June 1, 2023
A novel PANI@CS solid-phase dispersive extractant combined with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for the first time, which used high-throughput, multi-component, real-time online rapid pretreatment and quantitative classification of 16 mycotoxins from five different medicinal parts 13 genuine traditional Chinese medicines (TCMs). Ultra performance triple quadrupole separation ESI detection. An internal standard isotope matching calibration quantification purposes to compensate matrix effects. The limits detection (LOD) ranged 0.1 6.0 μg/kg. linear coefficients (R2) were ≥0.996 in range 10.0 200 μg/L. recoveries 90.1% 105.8%, relative deviations (RSDs) 1.3% 4.1%. Thirteen TCMs representative selected tested under best sample preparation procedure chromatographic analysis conditions. results showed that method could improve sensitivity accuracy analysis, selectivity reproducibility decolorization purification TCMs, is suitable practical application mycotoxin trace analysis. This can also provide a new idea accurate, efficient, multi-component quality safety control TCMs.
Language: Английский
Citations
2
International Journal of Environmental & Analytical Chemistry, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 14
Published: Oct. 1, 2023
ABSTRACTAn efficient, novel and green Graphene Oxide-Poly-L-Lysine (GO-PLL) adsorbent was successfully synthesised used for solid-phase extraction of aflatoxin M1 (AFM1) in liquid milk samples followed by high performance chromatography (HPLC) equipped with fluorescence detector (FLD). The characterised Fourier Transform Infrared Spectrophotometry (FT-IR), Field Emission Scanning Electron Microscopy (FESEM), Energy-dispersive X-ray spectroscopy (EDX) X-Ray diffraction (XRD) analysis. Different parameters affecting the recovery percentage AFM1 including pH sample solution, amounts adsorbent, contact time desorbent solution (types volume) were completely investigated optimised. It found that, an enrichment factor 15.2, calibration curve is linear range 0.05–4.00 ng ml−1 limit detection (LOD) quantification (LOQ) values 0.019 0.063 mL−1, respectively. relative standard deviations (RSDs) ranged from 4.5% to 5.7% 4.2% 6.7% intra-day inter-day variations, Finally, proposed method applied determination trace levels different samples.KEYWORDS: Aflatoxin M1Graphene adsorbentHPLCmilk AcknowledgmentsThe authors wish thank University Neyshabur financial support this project (grant number: 14703).Disclosure statementNo potential conflict interest reported author(s).Additional informationFundingThis work supported Medical Sciences [grant number:14703].
Language: Английский
Citations
2
Food Additives & Contaminants Part A, Journal Year: 2023, Volume and Issue: 40(11), P. 1470 - 1481
Published: Oct. 20, 2023
Contamination of ochratoxin A (OTA) is a common concern for the quality and safety licorice its derivatives, while their complex sample matrices always restrict monitoring regulation OTA. Taking much more concentrated complicated extract as representative, modified analysis method was established OTA by HPLC. Parameters were comprehensively investigated based on liquid-liquid extraction immunoaffinity column clean-up. In comparison to other methods, developed achieved effective clean-up efficiency selectivity without tedious procedures specialized instrumentation. Good linearity (R2 ≥0.9995), low LOD/LOQ (0.10 μg/kg/0.33 μg/kg), satisfactory recovery (90.0%-96.4%, RSDs <7.0%) indicated sensitivity reliability method. addition, applicability robustness demonstrated large numbers samples. It noteworthy that 66.5% 176 samples contaminated with OTA, concentrations 9.1% exceeded maximum limit (ML, 80 μg/kg) defined EU. On account high contamination frequency broad concentration range daily intake preliminarily determined be 123.18-123.93 g/day (chronic exposure) 24.24 (acute exposure), indicating potential acute risk through exposure. This calls improved supervision in study suggests prospective option efficient determination routine simultaneously providing valuable data base health assessment.
Language: Английский
Citations
2
Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown
Published: Jan. 1, 2023
Language: Английский
Citations
1