Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1732, P. 465222 - 465222
Published: Sept. 1, 2024
Language: Английский
Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(16)
Published: Aug. 1, 2024
This review provides an overview of recent works focusing on the determination amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection ultraviolet (UV) detection, which is most widespread in electromigration techniques, without pre-capillary derivatization. Available options for UV these analytes, such as indirect complexation transition metal ions, in-capillary derivatization are described. Developments field direct UV-absorbing AAs well progress chiral separation A separate section dedicated to on-line sample preconcentration methods combined electrophoresis-UV.
Language: Английский
Citations
2
Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 485 - 498
Published: Jan. 1, 2024
Language: Английский
Citations
1
Published: July 30, 2024
The extraction, separation, and purification of dietary proteins from a variety food sources are crucial for their targeted use in applications. To achieve this, should be effectively separated non-protein components such as cell wall structures, polysaccharides, lipids. Traditional protein methods can time-consuming, highlighting the need automated, cost-effective, sustainable alternatives. This comprehensive review critically assesses various instruments an analytical perspective, weighing advantages disadvantages. under evaluation include ultrafiltration, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), fast liquid chromatography (FPLC), high-performance (HPLC), ultra performance (UPLC), microfluidic chips. Among these, FPLC stands out affordable efficient technique that allows high recovery. However, HPLC UPLC provide faster results but may denature proteins, leading to lower recovery rates. Ultrafiltration is cost-effective straightforward method doesn’t require complex equipment. Microchip-based approaches emerging innovative techniques rapidly analyzing small samples. While SDS-PAGE user-friendly, it denatures particularly those linked other biomolecules. choice most appropriate instrument depends on factors cost, energy efficiency, processing time, characteristics target protein, desired outcomes, recovery, resource availability. By examining these purification, this aims assist researchers practitioners selecting suitable specific needs, ultimately promoting successful endeavors field science technology.
Language: Английский
Citations
1
Small Methods, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 29, 2024
Abstract Low‐input proteomics, also referred to as micro‐ or nanoproteomics, has become increasingly popular it allows one elucidate molecular processes in rare biological materials. A major prerequisite for the analytics of minute protein amounts, e.g., derived from low cell numbers, down single cells, is availability efficient sample preparation methods. Digital microfluidics (DMF), a technology allowing handling and manipulation liquid volumes, recently been shown be powerful versatile tool address challenges low‐input proteomics. Here, an overview provided on recent advances proteomics using DMF. In particular, capability DMF isolate proteomes cells small model organisms, perform all necessary chemical steps, such denaturation proteolytic digestion on‐chip, are highlighted. Additionally, prerequisites making these steps compatible with follow‐up analytical methods chromatography‐mass spectrometry will discussed.
Language: Английский
Citations
1
Electrophoresis, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 7, 2024
ABSTRACT This review provides an in‐depth exploration of capillary electrophoresis–mass spectrometry (CE–MS) in biomolecular research from 2020 to 2024. CE–MS emerges as a versatile and powerful tool due its numerous advantages, facilitating the analysis various biomolecules, including proteins, peptides, oligonucleotides, other metabolites, such lipids, carbohydrates, or amines, among others. The extends CE modes interfaces for coupling, offering comprehensive insights into their applications within research. Furthermore, it effectively summarizes conditions employed while also addressing critical aspects sample preparation requirements. Despite highlights gap between discovery practical implementation, underscoring need large‐scale validation method standardization fully realize potential
Language: Английский
Citations
1
Electrophoresis, Journal Year: 2024, Volume and Issue: unknown
Published: May 13, 2024
Abstract Therapeutic peptides have emerged as an innovative and promising class of therapeutic compounds in modern medicine. Synthetic peptide analogs triptorelin lanreotide are known for their pronounced clinical versatility potency. In this study, we present the development validation novel methods based on capillary zone electrophoresis performed hydrodynamically closed system (HCS) paired with ultraviolet detection repeated injection sample introduction. To best our knowledge, developed first electrophoresis‐based method determination lanreotide, concurrently, HCS triptorelin. Maximal separation efficiency signal intensity were achieved using background electrolytes composed 50 mM formic acid addition 0.05% (v/v) methyl‐hydroxyethyl cellulose. The proposed exhibit favorable performance characteristics, namely, calibration curve ( r 2 exceeding 0.99), low limits (0.25 µg/mL a water matrix 0.5 synthetic urine), acceptable precision (relative standard deviation ranging from 2.2% to 9.6% intraday repeatability between 5.2% 14.9% interday reproducibility), accuracy errors falling within 91.1%–107.8% range). was then used its quantification commercially available drug dosage form (powder injection) spiked urine samples. also evaluated according blue applicability grade index, revealing superior applicability. results collectively point out potential both quality control investigations.
Language: Английский
Citations
1
Chromatography, Journal Year: 2023, Volume and Issue: 44(3), P. 145 - 149
Published: Oct. 17, 2023
To improve the concentration sensitivity of cationic analytes in microchip electrophoresis, on-line sample preconcentration by a large-volume stacking with an electroosmotic flow pump (LVSEP) was performed using non-aqueous background solutions (BGSs) polymer-coated microchannels. In LVSEP analyses dimethyl sulfoxide-based BGS, however, poly(vinyl alcohol)/poly(allylamine) mixture-coated microchannel gave insufficient enrichments and broader peaks for histamine. By employing methanolic BGS containing (hydroxypropyl)methyl cellulose, on other hand, good histamine were achieved sensitive enhancement factor 67. our knowledge, this is first time that successfully realized media.
Language: Английский
Citations
3
Electrophoresis, Journal Year: 2023, Volume and Issue: 45(7-8), P. 735 - 742
Published: Dec. 12, 2023
Abstract Ion mobility–mass spectrometry (IM–MS) is an ever‐evolving tool to separate ions in the gas phase according electrophoretic mobility with subsequent mass determination. CE rarely coupled IM–MS, possibly due similar separation mechanisms based on mobility. Here, we investigate orthogonality of and ion (IM) by analyzing a complex peptide mixture (tryptic digest HeLa proteins) trapped (TIMS–MS). Using nanoCEasy interface, excellent sensitivity was achieved identifying thousands peptides achieving peak capacity 7500 (CE: 203–323 150 cm long capillary, IM: 27–31). Plotting versus (inverse) mobility, clear grouping curved striped patterns observed charge‐to‐size mass‐to‐charge ratios. The charge acidic background electrolyte can be estimated from number basic amino acids, few exceptions where neighboring effects reduce positive charge. A surprisingly high IM observed, which obviously caused solvation leading different charges sizes liquid compared phase. expected for other samples as well substance classes, making CE–IM–MS promising various applications.
Language: Английский
Citations
3
Green Analytical Chemistry, Journal Year: 2024, Volume and Issue: 10, P. 100141 - 100141
Published: Aug. 23, 2024
Language: Английский
Citations
0
Chromatography, Journal Year: 2024, Volume and Issue: 45(3), P. 107 - 112
Published: Aug. 29, 2024
In our previous study, we found that the combination of large-volume sample stacking with an electroosmotic flow pump (LVSEP) and transient isotachophoresis (tITP), which named large volume dual preconcentration by (LDIS), was successfully applied to microchip electrophoresis (MCE). MCE-LDIS, highly viscous background electrolytes (BGEs) containing sieving polymers such as hydroxypropyl(methyl cellulose) (HPMC) could be employed, should effective for concentrating separating especially bio-polymeric compounds proteins glycans. To adjust both enrichment efficiencies resolutions in this effects methanol HPMC additives BGE were investigated. no HPMC, peak height a standard dye increased upon increasing contents from 0 20%, while 20 60% gradually became smaller. Since viscosity water addition 20% 1.0 1.6 cP, band broadening focused analytes would suppressed, improving sensitivity enhancement factor (SEF) 1550 8250. Furthermore, use 10% 1.5% LDIS gave best SEF 18300 dye. Addition also separation oligosaccharides, 0.85~1.25 1.56~1.73. It revealed that, therefore, polymer quite important adjusting SEFs MCE-LDIS.
Language: Английский
Citations
0