Cryobiology, Journal Year: 2022, Volume and Issue: 106, P. 39 - 47
Published: May 1, 2022
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(8), P. 2781 - 2781
Published: April 16, 2020
Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm in different ruminant species. However, significant amount still suffers considerable cryodamage, which may affect quality and fertility. Recently, use "omics" technologies cryobiology, especially proteomics studies, has led better understanding molecular modifications induced by cryopreservation, facilitating identification freezability biomarkers certain proteins that can be added before enhance cryosurvival. This review provides an updated overview mechanisms involved are part responsible structural, functional fertility changes observed frozen-thawed sperm. Moreover, basis those factors freezing resilience species is also discussed as well aspects novel strategies developed reduce including new cryoprotectants, antioxidants, proteins, nanoparticles vitrification.
Language: Английский
Citations
186
Animal Reproduction Science, Journal Year: 2021, Volume and Issue: 246, P. 106904 - 106904
Published: Dec. 3, 2021
Sperm cryopreservation is one of the most important procedures in development biotechnologies for assisted reproduction. In some farm animals, use cryopreserved sperm has so many benefits which relevance become more evident recent decades. Values post-thaw quality, however, are variable among species and within individuals same species. There no standardized methodology each stages procedure (andrological examination, semen collection, dilution, centrifugation, resuspension pellet with freezing medium, packaging, evaluation), also contributes to differences studies. Cryotolerance markers seminal plasma (SP) have been evaluated prediction ejaculate freezability. addition, previous research, there a focus on supplementing media different substances, such as enzymatic non-enzymatic antioxidants. studies, inclusion these substances led improved quality fertilizing capacity result minimizing adverse effects structure function. Another approach cryoprotectants. The aim this review article provide an update animals. main detrimental described, including negative repercussion reproductive performance. Furthermore, potential molecular biomarkers predict cryotolerance discussed, well addition that can mitigate harmful impact thawing sperm.
Language: Английский
Citations
112
Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 624 - 624
Published: May 21, 2024
Cryopreservation poses significant challenges to the preservation of sperm integrity and function, particularly in small ruminants where cryodamage is pronounced. This review explores molecular mechanisms underlying strategies for improving cryopreservation outcomes, with a focus on role antioxidants. Cryopreservation-induced alterations proteins RNA transcripts critical including motility, capacitation, fertilization, embryo development, are discussed. Proteomic, transcriptomic, epigenomic advancements have provided valuable insights into these mechanisms, offering potential biomarkers predicting freezability enhancing strategies. Combining technologies such as mass spectrometry flow cytometry allows comprehensive understanding cellular changes induced by freezing–thawing process. However, remain optimizing cryoprotectant formulations antioxidant supplementation improve post-thaw fertility. Further research needed explore wider range novel cryoprotectants, antioxidants, media, well validate their efficacy viability function. Additionally, investigations effects epigenetic factors ruminant species warranted advance our preservation. Overall, this highlights importance antioxidants mitigating underscores need continued refine protocols reproductive outcomes ruminants.
Language: Английский
Citations
11
Theriogenology, Journal Year: 2024, Volume and Issue: 219, P. 167 - 179
Published: Feb. 26, 2024
Porcine seminal plasma (SP) is loaded with a heterogeneous population of extracellular vesicles (sEVs) that modulate several reproductive-related processes. This study investigated the effect two sEV subsets, small (S-sEVs) and large (L-sEVs), on porcine in vitro fertilization (IVF). The sEVs were isolated from nine SP pools (five ejaculates/pool) using size-exclusion chromatography-based procedure characterized for quantity (total protein), morphology (cryogenic electron microscopy), size distribution (dynamic light scattering), purity EV-protein markers (flow cytometry; albumin, CD81, HSP90β). characterization confirmed existence subsets high (low albumin content) differed (S- L-sEVs). In was performed matured oocytes frozen-thawed spermatozoa IVF medium supplemented during gamete coincubation (1 h at 38.5 °C, 5 % CO2 humidified atmosphere) three different concentrations each subset: 0 (control, without sEVs), 0.1, 0.2 mg/mL. first experiment showed sEVs, regardless subset concentration, decreased penetration rates total efficiency (P < 0.0001). subsequent experiment, it shown impaired ability to bind zona pellucida following subset, bound sperm but not oocytes, indicating would affect functionality oocyte functionality. lack by incubating prior IVF, achieving sperm-zona binding results similar those control. last conducted under conditions, analyzed terms tyrosine phosphorylation, acrosome integrity metabolism. did phosphorylation or integrity, influence metabolism decreasing ATP production capacitating conditions. conclusion, this demonstrated presence impairs outcomes, most likely altering
Language: Английский
Citations
9
Andrologia, Journal Year: 2021, Volume and Issue: 53(8)
Published: June 18, 2021
Sperm cryopreservation is an important adjunct to assisted reproduction techniques (ART) for improving the reproductive efficiency of dairy cattle and buffaloes. Improved understanding mechanisms challenges bovine semen vital artificial insemination on a commercial basis. Although spermatozoa widely practiced advanced beyond that other species, there are still major gaps in knowledge technology. Upon cryopreservation, disruption spermatozoal plasma membrane configuration due alterations metabolic pathways, enzymes antioxidants activity add lower with loss sperm longevity fertilising ability. Therefore, effective amalgamation cryo-variables like ambient temperature, cooling thawing rates, nucleation type concentration cryoprotectant, seminal composition, free radicals antioxidant status required optimise cryopreservation. Novel strategies supplementation cholesterol-loaded cyclodextrins (CLC), nanovesicles, osteopontin, antioxidants, etc., extender recent nano-purification modified packaging have be optimised ameliorate cryodamage. This article intended describe basic facts about process associated biochemical, biophysical, ultra-structural, molecular functional alterations.
Language: Английский
Citations
42
Journal of Proteomics, Journal Year: 2020, Volume and Issue: 221, P. 103765 - 103765
Published: April 2, 2020
Language: Английский
Citations
43
Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)
Published: Sept. 5, 2019
Sperm cryopreservation is an important tool for storing genetic traits and assisted reproduction techniques. Several studies have developed semen protocols. However, the sperm proteome different between ejaculated epididymal spermatozoa little known about effects on spermatozoa. Therefore, our study aimed to (i) investigate differences of parameters based freezing tolerance (ii) identify potential markers predict freezability bull Our preliminary demonstrated that from individual bulls differ in freezability. We categorized into high freezing-tolerant low group motility after freezing/thawing. evaluated several functional parameters, including motility/motion kinematics, speed viability, mitochondrial activity, capacitation status. results motility, membrane had significant two groups but motion kinematics status did not. In addition, concentration three proteins - glutathione s-transferase mu 5, voltage-dependent anion-selective channel protein 2, ATP synthase subunit beta, differed both groups. Thus, research highlighted upon these might be useful select
Language: Английский
Citations
42
Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9
Published: July 16, 2021
Sperm cells are of unique elongated structure and function, the development which is tightly regulated by existing proteins posttranslational modifications (PTM) these proteins. Based on phylogenetic relationships various swine breeds, Yorkshire boar believed to be distinctly different from Duroc boar. The comprehensive differential proteomics phosphoproteomics profilings were performed spermatozoa both boars. By peptide PTM quantification followed statistical analyses, 167 differentially expressed identified 1,745 proteins, 283 phosphopeptides corresponding 102 phosphorylated measured 1,140 derived 363 representative results validated Western blots. Pathway enrichment analyses revealed that majority expression phosphorylation primarily concerned with spermatogenesis, male gamete generation, sperm motility, energy metabolism, cilium morphogenesis, axonemal dynein complex assembly, sperm–egg recognition, capacitation. Remarkably, assembly related such as SMCP, SUN5, ODF1, AKAP3, AKAP4 play a key regulatory role in physiological functions, significantly higher than Yorkshire. Furthermore, sperm-specific CABYR, ROPN1, CALM1, PRKAR2A, PRKAR1A, participates regulation motility mainly through cAMP/PKA signal pathway demonstrating protein may an important mechanism underlying diversity. Protein–protein interaction analysis 14 overlapped between potentially played flagellum, including AKAP4, FSIP2, SUN5. Taken together, physiologically functionally (DEPs) (DPPs) constitute proteomic backgrounds two breeds. validation will delineate roles modulators spermatozoa.
Language: Английский
Citations
31
Animals, Journal Year: 2023, Volume and Issue: 13(14), P. 2300 - 2300
Published: July 13, 2023
The present review aims to provide an overview of the assay methods for quantification ROS and principal enzymatic antioxidants as biomarkers oxidative stress in erythrocytes spermatozoa small domestic ruminants. A complete literature search was carried out PubMed, Scopus World Wide Web using relevant keywords focusing on last five years (2018-2023). Among spectrophotometry, fluorometry chemiluminescence, most widely used method is fluorometry, probably because it allows simultaneously several ROS, different probes, with greater economic advantages. Regarding intracellular antioxidant enzymes, recent reports only spectrophotometric methods, many which use commercial kits. a less sensitive but cheapest suitable both samples are highly concentrated ruminant species. All considered this have been found be appropriate; general, differences related their costs sensitivity. Quantification activity may find application study welfare health status ruminants monitoring livestock production.
Language: Английский
Citations
11
Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 263, P. 107441 - 107441
Published: Feb. 21, 2024
Language: Английский
Citations
4