Engineering injectable, biocompatible, and highly elastic bioadhesive cryogels DOI Creative Commons
Devyesh Rana, Thibault Colombani,

Bahram Saleh

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 19, P. 100572 - 100572

Published: Feb. 1, 2023

The extracellular matrix (ECM), an integral component of all organs, is inherently tissue adhesive and plays a pivotal role in regeneration remodeling. However, man-made three-dimensional (3D) biomaterials that are designed to mimic ECMs do not intrinsically adhere moisture-rich environments often lack open macroporous architecture required for facilitating cellularization integration with the host post-implantation. Furthermore, most these constructs usually entail invasive surgeries potentially risk infection. To address challenges, we recently engineered biomimetic cryogel scaffolds syringe injectable while exhibiting unique physical properties, including strong bioadhesive properties tissues organs. These catechol-containing cryogels were prepared from naturally-derived polymers such as gelatin hyaluronic acid functionalized mussel-inspired dopamine (DOPA) impart properties. We found using glutathione antioxidant incorporating DOPA into via PEG spacer arm led highest adhesion improved overall, whereas DOPA-free weakly adhesive. As shown by qualitative quantitative tests, DOPA-containing able strongly several animal organs heart, small intestine, lung, kidney, skin. unoxidized (i.e., browning-free) showed negligible cytotoxicity toward murine fibroblasts prevented

Language: Английский

Progress in Gelatin as Biomaterial for Tissue Engineering DOI Creative Commons
Izeia Lukin,

Itsasne Erezuma,

Lidia Maeso

et al.

Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(6), P. 1177 - 1177

Published: May 31, 2022

Tissue engineering has become a medical alternative in this society with an ever-increasing lifespan. Advances the areas of technology and biomaterials have facilitated use engineered constructs for issues. This review discusses on-going concerns latest developments widely employed biomaterial field tissue engineering: gelatin. Emerging techniques including 3D bioprinting gelatin functionalization demonstrated better mimicking native by reinforcing gelatin-based systems, among others. breakthrough facilitates, on one hand, manufacturing process when it comes to practicality cost-effectiveness, which plays key role transition towards clinical application. On other can be concluded that could considered as promising future trends, focus might detection diagnosis diseases rather than treatment.

Language: Английский

Citations

152
Living and Injectable Porous Hydrogel Microsphere with Paracrine Activity for Cartilage Regeneration DOI

Xingchen Li,

Xiaoxiao Li, Jielai Yang

et al.

Small, Journal Year: 2023, Volume and Issue: 19(17)

Published: Jan. 18, 2023

Abstract Paracrine is an important mechanism in mesenchymal stem cells (MSCs) that promotes tissue regeneration. However, anoikis attributed to unsuitable adhesion microenvironment hindered this paracrine effect. In study, a living and injectable porous hydrogel microsphere with long‐term activity constructed via the freeze‐drying microfluidic technology incorporation of platelet‐derived growth factor‐BB (PDGF‐BB) exogenous MSCs. Benefiting from structure superior mechanical property methacrylate gelatin (GelMA) microspheres (GMs), are able adhere proliferate on GMs, thereby facilitating cell‐to‐extracellular matrix (ECM) cell‐to‐cell interactions enhancing Furthermore, sustained release PDGF‐BB can recruit endogenous MSCs prolong GMs. vitro vivo experiments validated GMs exhibit secretion properties anti‐inflammatory efficacy attenuate osteoarthritis (OA) progression by favoring adherent utilizing synergistic effect Overall, enhance fabricated anticipated hold potential future clinical translation OA other diseases.

Language: Английский

Citations

79
Bioinspired porous microspheres for sustained hypoxic exosomes release and vascularized bone regeneration DOI Creative Commons

Yike Gao,

Zuoying Yuan, Xiaojing Yuan

et al.

Bioactive Materials, Journal Year: 2022, Volume and Issue: 14, P. 377 - 388

Published: Feb. 1, 2022

Exosomes derived from mesenchymal stem cells (MSCs) have demonstrated regenerative potential for cell-free bone tissue engineering, nevertheless, certain challenges, including the confined therapeutic potency of exosomes and ineffective delivery method, are still persisted. Here, we confirmed that hypoxic precondition could induce enhanced secretion human exfoliated deciduous teeth (SHEDs) via comprehensive proteomics analysis, corresponding (H-Exo) exhibited superior in promoting cellular angiogenesis osteogenesis significant up-regulation focal adhesion, VEGF signaling pathway, thyroid hormone synthesis. Then, developed a platform technology enabling effective with sustained release kinetics to irregular-shaped defects injection. This is based on simple adsorbing technique, where adsorbed onto surface injectable porous poly(lactide-co-glycolide) (PLGA) microspheres bioinspired polydopamine (PDA) coating (PMS-PDA microspheres). The PMS-PDA effectively adsorb exosomes, show H-Exo 21 days high bioactivity, vascularized regeneration 5-mm rat calvarial defect. These findings indicate microsphere-based exosome efficient inducing regeneration, hence facilitating clinical translation exosome-based therapy.

Language: Английский

Citations

76
Young Exosome Bio‐Nanoparticles Restore Aging‐Impaired Tendon Stem/Progenitor Cell Function and Reparative Capacity DOI
Shanshan Jin, Yu Wang,

Xiaolan Wu

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(18)

Published: Feb. 13, 2023

Aging impairs tendon stem/progenitor cell function and homeostasis, however, effective treatments for aging-induced diseases are lacking. Exosomes naturally derived nanoparticles that contain bioactive molecules, therefore, have attracted great interest in tissue engineering regenerative medicine. In this study, it is shown young exosomes secreted by stem cells from human exfoliated deciduous teeth (SHED-Exos) possess abundant anti-aging signals. These bio-nanoparticles can alleviate the aging phenotypes of aged (AT-SCs) maintain their tenogenic capacity. Mechanistically, SHED-Exos modulate histone methylation inhibit nuclear factor-κB to reverse AT-SC aging. a mouse model, systemic administration SHED-Exo retards degeneration. Interestingly, local delivery SHED-Exos-loaded microspheres confers phenotypes, including reduced senescent decreased ectopic bone formation, thereby functionally structurally rescuing endogenous regeneration repair capacity rats. Overall, SHED-Exos, as natural nanoparticles, promising translational therapeutic potential aging-related diseases.

Language: Английский

Citations

75
Smart Hydrogels for Bone Reconstruction via Modulating the Microenvironment DOI Creative Commons

Weikai Chen,

Hao Zhang,

Qirong Zhou

et al.

Research, Journal Year: 2023, Volume and Issue: 6

Published: Jan. 1, 2023

Rapid and effective repair of injured or diseased bone defects remains a major challenge due to shortages implants. Smart hydrogels that respond internal external stimuli achieve therapeutic actions in spatially temporally controlled manner have recently attracted much attention for therapy regeneration. These can be modified by introducing responsive moieties embedding nanoparticles increase their capacity repair. Under specific stimuli, smart variable, programmable, controllable changes on demand modulate the microenvironment promoting healing. In this review, we highlight advantages summarize materials, gelation methods, properties. Then, overview recent advances developing biochemical signals, electromagnetic energy, physical including single, dual, multiple types enable physiological pathological modulating microenvironment. discuss current challenges future perspectives regarding clinical translation hydrogels.

Language: Английский

Citations

58
Advanced strategies of scaffolds design for bone regeneration DOI Creative Commons
Jian Song, Longfei Li, Lei Fang

et al.

BMEMat, Journal Year: 2023, Volume and Issue: 1(4)

Published: Sept. 2, 2023

Abstract Bone defects are encountered substantially in clinical practice, and bionic scaffolds represent a promising solution for repairing bone defects. However, it is difficult to fabricate with structures reconstruct the microenvironment fulfill satisfying repair effects. In this review article, we first discuss various strategies design construction of promote defect repair, especially including structural scaffold integration bioactive substances together application external stimuli. We then roles artificial intelligence medical imaging aiding treatment. Finally, point out challenges future outlooks developing multifunctional scaffolds, aiming provide insights improving regeneration efficacy accelerating translation.

Language: Английский

Citations

51
Leveraging the Recent Advancements in GelMA Scaffolds for Bone Tissue Engineering: An Assessment of Challenges and Opportunities DOI
Narsimha Mamidi,

Fatemeh Ijadi,

Mohammad Hadi Norahan

et al.

Biomacromolecules, Journal Year: 2023, Volume and Issue: 25(4), P. 2075 - 2113

Published: July 5, 2023

The field of bone tissue engineering has seen significant advancements in recent years. Each year, over two million transplants are performed globally, and conventional treatments, such as grafts metallic implants, have their limitations. Tissue offers a new level treatment, allowing for the creation living within biomaterial framework. Recent advances biomaterials provided innovative approaches to rebuilding function after damage. Among them, gelatin methacryloyl (GelMA) hydrogel is emerging promising supporting cell proliferation regeneration, GelMA exhibited exceptional physicochemical biological properties, making it viable option clinical translation. Various methods classes additives been used application with incorporation nanofillers or other polymers enhancing its resilience functional performance. Despite results, fabrication complex structures that mimic architecture provision balanced physical properties both vasculature growth proper stiffness load bearing remain challenges. In terms utilizing osteogenic additives, priority should be on versatile components promote angiogenesis osteogenesis while reinforcing structure applications. This review focuses efforts advantages GelMA-based composite engineering, covering literature from last five

Language: Английский

Citations

48
Tough, self-healing, and injectable dynamic nanocomposite hydrogel based on gelatin and sodium alginate DOI
Li Gao, Yuan He,

Yasir Aziz

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: unknown, P. 121812 - 121812

Published: Jan. 1, 2024

Language: Английский

Citations

47
Structure and Properties of Gelatin Methacryloyl (GelMA) Synthesized in Different Reaction Systems DOI
Shangsi Chen, Yue Wang,

Jiahui Lai

et al.

Biomacromolecules, Journal Year: 2023, Volume and Issue: 24(6), P. 2928 - 2941

Published: May 22, 2023

Gelatin methacryloyl (GelMA) hydrogels have been extensively used for drug delivery and tissue engineering applications due to their good biocompatibility, biodegradability, controllable photocurable efficiency. Phosphate buffer solution (PBS) is the most widely reaction system GelMA synthesis. However, carbonate–bicarbonate (CBS) has tried recently synthesizing its high there a lack of systematic investigation into possible differences in structure properties synthesized PBS CBS, respectively. Therefore, current study, molecules with two degrees methacryloylation (∼20 ∼80%) were under CBS systems, respectively, comparable conditions. The results showed that because functionalization methacrylate groups gelatin chains, which could interfere intrachain interchain interactions, such as hydrogen bonding, had distinct physical structures exhibited different comparison those produced CBS. higher gel–sol transition temperatures better efficiencies, mechanical strength, biological properties. In contrast, advantages swelling performance microstructures, pore sizes porosities. addition, possessing degree (the "GelMA-PH" polymer) great potential three-dimensional (3D) bioprinting. This focused study gained helpful new insights can provide guidance on application 3D printing engineering.

Language: Английский

Citations

45
Injectable, High Specific Surface Area Cryogel Microscaffolds Integrated with Osteoinductive Bioceramic Fibers for Enhanced Bone Regeneration DOI
Yue Wang, Zuoying Yuan,

Yanyun Pang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(17), P. 20661 - 20676

Published: April 21, 2023

Organic-inorganic composites with high specific surface area and osteoinductivity provide a suitable microenvironment for cell ingrowth effective ossification, which could greatly promote bone regeneration. Here, we report gelatin methacryloyl (GelMA) cryogel microspheres that are reinforced hydroxyapatite (HA) nanowires calcium silicate (CS) nanofibers to achieve the goal. The prepared composite open porous structure rough facilitate anchoring, simultaneously exhibiting excellent injectability. Compared only HA- or CS-containing counterparts, GelMA composited HA:CS (termed as GMHC) sustained release of bioactive Ca, P, Si elements, conducive osteogenic differentiation marrow mesenchymal stromal cells (BMSCs). These can prevent from forming peralkalic conditions, is beneficial growth. After injection into rat calvarial defects, neo-bone tissue grows their pores, showing tight integration. embedded bioceramic components significantly regeneration, GMHC achieving best regenerative outcomes. Promisingly, organic-inorganic microspheres, area, biodegradability, osteoinductivity, act injectable microscaffolds repair defects enhanced efficiency, may widen scaffold strategy engineering.

Language: Английский

Citations

43